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OfflineShroomterd
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Re: Cultivation General Discussion [Re: fahtster]
    #27301511 - 05/09/21 11:18 AM (2 years, 8 months ago)

thanks I'm in your tek now..


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OfflineInthepit
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Re: Cultivation General Discussion [Re: Josex]
    #27301524 - 05/09/21 11:33 AM (2 years, 8 months ago)

Quote:

Josex said:
Yea I also rinse the swab in sterile water like Faht does. This does 2 things, cleans it up a bit and softens the fibers so I can take tiny pieces off the swab with the tip of the scalpel blade. Then I bury each piece I take in separate BRF/verm pucks. .



That sounds very straight forward, fewest steps to a fruit.
Of course for LAGM we had to use agar. But did you notice how many of us
had trouble with swabs? I never made it with mine.
This looks really cool!  :awedance:


--------------------
:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:


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InvisibleJosex
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Re: Cultivation General Discussion [Re: Inthepit]
    #27301533 - 05/09/21 11:41 AM (2 years, 8 months ago)

Quote:

Inthepit said:
Quote:

Josex said:
Yea I also rinse the swab in sterile water like Faht does. This does 2 things, cleans it up a bit and softens the fibers so I can take tiny pieces off the swab with the tip of the scalpel blade. Then I bury each piece I take in separate BRF/verm pucks. .



That sounds very straight forward, fewest steps to a fruit.
Of course for LAGM we had to use agar. But did you notice how many of us
had trouble with swabs? I never made it with mine.
This looks really cool!  :awedance:




Well then I cheated for my LAGM lol
I used pucks, no agar. That was my way to kill the cat, or skin it, whatever...

As I understand it, the rule about putting the spores to agar first is there to avoid getting people shooting them directly into grain, right?
I used an agar substitute, which serves the same purpose, which is germinating spores to obtain clean myc to work with. Hope that ain't cheating :lol:


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InvisiblefahtsterM
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Re: Cultivation General Discussion [Re: Josex]
    #27301548 - 05/09/21 11:57 AM (2 years, 8 months ago)

I think the idea is to get new ppl into agar because it can be intimidating to go at alone, so I think you good.  No one probably even noticed :lol:


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Offlinelookintolearn
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Re: Cultivation General Discussion [Re: fahtster]
    #27301584 - 05/09/21 12:32 PM (2 years, 8 months ago)

So with my bunch of shittily cased PE I was wondering if it would be an idea (not sure if a good one lol) to just scrape the casing layer off and see what happens? Most of them are pretty much fucked anyways. Fuck using jiffy straight from the bag anymore :facepalm3:

With my two tubs that I hadn't cased yet I'm trying a thick CV layer and see how it goes. Next test with be a thick CV layer from spawning. Then going to try pasteruzing jiffy. See what works best.


--------------------
Don't be afraid of feeling the feelings

Lookin to LAGM 2021



Looking to start growing? Read through Bod's Introduction to Everything
Looking to start agar? Start with Alien's Holy Grail
Looking to perfect your transfers? Start with D3monic's Perfect Transfers
Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek
Looking to start LC? Try LI first! Munch's super easy Blenderless LI


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InvisiblefahtsterM
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Re: Cultivation General Discussion [Re: lookintolearn]
    #27301602 - 05/09/21 12:42 PM (2 years, 8 months ago)

I’ve vacuumed off shitty jiffy casing before.. it went bad the day after I applied it and just took my vacuum hose and sucked it right off :lol:

If you’re going to keep using a peat based casing (if the coir doesn’t work out) I’d suggest making your own.. it’s really easy and much cheaper.  If you haven’t seen it https://www.shroomery.org/forums/showflat.php/Number/27177370#27177370

Faht


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OfflineInthepit
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Re: Cultivation General Discussion [Re: lookintolearn]
    #27301643 - 05/09/21 01:28 PM (2 years, 8 months ago)

Quote:

lookintolearn said:
So with my bunch of shittily cased PE I was wondering if it would be an idea (not sure if a good one lol) to just scrape the casing layer off and see what happens? Most of them are pretty much fucked anyways. Fuck using jiffy straight from the bag anymore :facepalm3:

With my two tubs that I hadn't cased yet I'm trying a thick CV layer and see how it goes. Next test with be a thick CV layer from spawning. Then going to try pasteurizing jiffy. See what works best.




Sounds similar, here's my APEs after I scraped the casing layer off...
         
                      and before 

That's a second flush and they're still avoiding the center.
So I flipped two and we'll see...there's my casing Rx in LAGM21, probably how I screwed it up!


--------------------
:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:


Edited by Inthepit (05/09/21 01:47 PM)


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Offlinelookintolearn
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Re: Cultivation General Discussion [Re: fahtster]
    #27301648 - 05/09/21 01:36 PM (2 years, 8 months ago)

Quote:

fahtster said:
I’ve vacuumed off shitty jiffy casing before.. it went bad the day after I applied it and just took my vacuum hose and sucked it right off :lol:

If you’re going to keep using a peat based casing (if the coir doesn’t work out) I’d suggest making your own.. it’s really easy and much cheaper.  If you haven’t seen it https://www.shroomery.org/forums/showflat.php/Number/27177370#27177370

Faht





Hahah honestly that's a good idea. Less damage probably to the mycelium underneath. After the "first flush" gets done I'll get rid of that and see what happens. I've had 5 tubs going for a second flush trich out and another one get some sort of fluffy white mold. Big ole oooof.

And yessss that link is what I needed gonna bookmark that for later thanks homie.

And put I'm hoping I can get some second flush out of my tubs they are all 66qt monos putting out like 5-15 fruits for the whole tub first flush
Big waste of time and effort all because I was being lazy lol.


--------------------
Don't be afraid of feeling the feelings

Lookin to LAGM 2021



Looking to start growing? Read through Bod's Introduction to Everything
Looking to start agar? Start with Alien's Holy Grail
Looking to perfect your transfers? Start with D3monic's Perfect Transfers
Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek
Looking to start LC? Try LI first! Munch's super easy Blenderless LI


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Offlinesmalltalk_canceled
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Re: Cultivation General Discussion [Re: lookintolearn]
    #27301894 - 05/09/21 05:39 PM (2 years, 8 months ago)

I will try the swab teks with the ape swab, after reading these enthusiastic defense of the pious swab. Thanks for loving care as always mycelium brothers

I mean if they are so good, they are saving time since you'll have to less cleaning up on plates

A similar thing I do with dirty stuff is the double transfer: do a normal transfer, wait for air to settle, then transfer the same piece or a piece of the piece to. New dish. I've seen this cut down on transfers to escape contams, and it's simple to do and requires no additional prep or items.



--------------------
Willpower is the one true virtue



Edited by smalltalk_canceled (05/09/21 10:13 PM)


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InvisibleQM33
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Re: Cultivation General Discussion [Re: fahtster]
    #27301906 - 05/09/21 05:49 PM (2 years, 8 months ago)

Quote:

fahtster said:
No... I go into detail in that link I posted but I’ll give ya the quick run through.  I sterilize two small glasses of water.. one is for rinsing the swabs, the other is for the spore solution (the second one has about 10-15ml of water only).  I take two swabs that have been dried for at least a week.. dip them a few times in the first jar of water to rinse them (I’ll usually rub them together once lightly where the spores are) then I take them to the other jar of sterile water and rub them together where the spores are vigorously to dislodge the spores into the water... then I suck up that water with a sterile syringe and use that to inoculate cakes or small grain jars. 

I think josex just drips some into his puck jars, which is something I’m going to start doing for my small grain jars.. cuts out the need to inoculate through the SHIP which is an added vector

Faht




I was thinking of doing something like this ha. I should have listened to some advice got the other day, I tried to cut the swab with a razor blade and should not have ha, it was fine but not what I wanted.
I've also read Josexs swab approach,and I should have done that over what I did.

But then I was thinking I could have sterilized the water, and either got a blender attachkent(probably to much for me), or what if I just sterilized water, and put the tip, or whole, tip in the jar, let it dissolve, then come back and put it in a syringe?

I see you tho faht!


--------------------
OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum...
There are known knowns, there are known unknowns,
          there are also unknown unknowns.
With great privilege comes great responsibility.

Quantom Qups PROOF AND Soft Drops
Turn your Swab to a Syringe and Syringe to Multiple Syringes!
No Pours (QuantomStyal)
Magic Fruit Leather
DMT for IandI


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OfflineSpaceBaby
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Re: Cultivation General Discussion [Re: QM33]
    #27302095 - 05/09/21 08:35 PM (2 years, 8 months ago)

dafuq? birth em or toss em. Noob eyes aren't much help yet.

B  Jar1

[image]http://www.shroomery.org/forums/thumbs/21-18/058364644-thumb_B_jar1_pic-2.jpg[/image]





GT Jar 3 This sure doesn't look right to me. Dafuq I know..









Before I finnd more challenges, I think the agar is looking good:

Malabar 1st generation agar from MSS


:rockon:

Back to DAFUQ is going on

GT Jar2 I think bacterial based on stalling this way. The red and green lines I drew to gauge growth











I got a tip that I might get some answers in this thread.

TYIA


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



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Offlinetiptrippy
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Re: Cultivation General Discussion [Re: SpaceBaby]
    #27302121 - 05/09/21 08:59 PM (2 years, 8 months ago)

@SpaceBaby

I would birth all 3 and cut out the uncolonized portion on GT jar 2. Nice agar dish! Send that one to grain!


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: tiptrippy]
    #27302181 - 05/09/21 09:52 PM (2 years, 8 months ago)

Bacterium


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OfflineFailboat
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #27302198 - 05/09/21 10:12 PM (2 years, 8 months ago)

I make a couple dollar signs when I steak my swab then snap off the tip deep in the last one. I get good germ.


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Offlinesmalltalk_canceled
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Re: Cultivation General Discussion [Re: Failboat]
    #27302285 - 05/10/21 12:08 AM (2 years, 8 months ago)

Paid up by the q tip companies.

One thing is for sure natedog, I support  brf as a "agar" like you call it with ya pucks n shit. Brf is the classic,
Can't mess with classics.

Flex that brf bro




--------------------
Willpower is the one true virtue



Edited by smalltalk_canceled (05/10/21 01:07 AM)


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InvisiblefahtsterM
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Re: Cultivation General Discussion [Re: lookintolearn]
    #27302335 - 05/10/21 02:01 AM (2 years, 8 months ago)

Quote:

lookintolearn said:
Quote:

fahtster said:
I’ve vacuumed off shitty jiffy casing before.. it went bad the day after I applied it and just took my vacuum hose and sucked it right off :lol:

If you’re going to keep using a peat based casing (if the coir doesn’t work out) I’d suggest making your own.. it’s really easy and much cheaper.  If you haven’t seen it https://www.shroomery.org/forums/showflat.php/Number/27177370#27177370

Faht





Hahah honestly that's a good idea. Less damage probably to the mycelium underneath. After the "first flush" gets done I'll get rid of that and see what happens. I've had 5 tubs going for a second flush trich out and another one get some sort of fluffy white mold. Big ole oooof.

And yessss that link is what I needed gonna bookmark that for later thanks homie.

And put I'm hoping I can get some second flush out of my tubs they are all 66qt monos putting out like 5-15 fruits for the whole tub first flush
Big waste of time and effort all because I was being lazy lol.




Of course, I took a picture of the vacuumed sub.. it was a second PE casing layer.. you can see where there’s bits of dark black casing left.. in the bottom right hand corner you can see where I got a little too close to the sub with the vacuum hose and silicones out a divet of both casing layers.  It’s only gonna work with loose casing mix though.. otherwise it’s gonna suck some sub up with it lol


Faht


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OfflineSpaceBaby
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Re: Cultivation General Discussion [Re: fahtster]
    #27302498 - 05/10/21 06:00 AM (2 years, 8 months ago)

@tiptrippy

Thanks.

Even that one with the fuzzy growth on the in vitro pins? Fingers crossed.


I have great agar growth on at least a coupe of plates of:
Malabar
B
GT
I have good plates of:
Huatla
McKennai
Treasure Coast

Put GT wedges into 7 jars of oats yesterday. Gonna nocc another 7 with Malabar now.

From orbit...


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: SpaceBaby]
    #27302522 - 05/10/21 06:32 AM (2 years, 8 months ago)

You guys ever heard of these anti trich bacteria products?


Supposedly you can add it to the sub at spawn and then spray bottle it over the walls and it creates a film of beneficial bacteria that locks mold out.

I mean it sounds like they took the surviving microbes from pasteurized manure and bred them so you could add them to coir or masters mix. Gotta admit I’m a little curious.

I thought for sure it was some bullshit til I read about it a little more to see how it works.


--------------------
LAGM2020


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InvisibleQM33
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Re: Cultivation General Discussion [Re: A.k.a]
    #27302525 - 05/10/21 06:36 AM (2 years, 8 months ago)

Like the dr myc shit?

https://www.drmyc.com/

I've been wanting to ask. I think other people have with little answering.
Sounds good. Sounds too good. And you would think this concept would have been picked up years ago?
And no feedback from any direction?

I'm saying snake oil furnoww

Isawreddit talking about it, willy myco I think. And even freshfarmfungi got some time try and never, as I could tell, said another word about it.


--------------------
OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum...
There are known knowns, there are known unknowns,
          there are also unknown unknowns.
With great privilege comes great responsibility.

Quantom Qups PROOF AND Soft Drops
Turn your Swab to a Syringe and Syringe to Multiple Syringes!
No Pours (QuantomStyal)
Magic Fruit Leather
DMT for IandI


Edited by QM33 (05/10/21 06:37 AM)


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: QM33]
    #27302535 - 05/10/21 06:47 AM (2 years, 8 months ago)

Beneficial bacteria has been used in the commercial mushroom industry in Asia for a good while, I even started a thread about it that didn't receive a very warm welcome, lol.

There have been some studies regarding the efficacy of bacteria as a prophylactic against Trichoderma in grain spawn.

This is the thread, if you're interested:

https://www.shroomery.org/forums/showflat.php/Number/26872959


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