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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 21 days
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Re: Cultivation General Discussion [Re: A.k.a]
#27289983 - 05/02/21 06:46 AM (2 years, 8 months ago) |
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I use old retired kithen towels in the bottom and they are orange pokadot speckled now.
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Cultivation General Discussion [Re: Sockadin]
#27289984 - 05/02/21 06:48 AM (2 years, 8 months ago) |
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You what's errbodies tapwater pH? Mines fuckin 10.0
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 4 hours, 17 minutes
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Re: Cultivation General Discussion [Re: sandman420]
#27289989 - 05/02/21 06:56 AM (2 years, 8 months ago) |
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Mines around 9.
Never bothered to check til I started some weed and they turned lime green/yellow.
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LAGM2020     
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 21 days
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Re: Cultivation General Discussion [Re: A.k.a]
#27289994 - 05/02/21 06:58 AM (2 years, 8 months ago) |
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I would hate to find out. Im on well water and it is terrible. 1600 PPM disolved solids.
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Cultivation General Discussion [Re: Sockadin]
#27289997 - 05/02/21 07:03 AM (2 years, 8 months ago) |
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wow thats a lotta minerals!
yea same for me about the weed. my water sucks so hard.
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starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 18 days
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Re: Cultivation General Discussion [Re: sandman420]
#27290103 - 05/02/21 08:26 AM (2 years, 8 months ago) |
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pH 7.7,
Low salts, very low turbidity, high chlorine but that's off-gassed readily in everything we do in this hobby due to heat. Tasty as fuck.
-------------------- Listen, I'm steel fisted with the iron lung Heavy metal ballads out the guitar where lions run.
 
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Cultivation General Discussion [Re: starbones]
#27290114 - 05/02/21 08:31 AM (2 years, 8 months ago) |
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Damn that sounds heavenly. I gotta fuckin move.
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gabbk
Metta cultivator


Registered: 03/06/20
Posts: 453
Last seen: 2 days, 5 hours
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Re: Cultivation General Discussion [Re: sandman420]
#27290295 - 05/02/21 11:35 AM (2 years, 8 months ago) |
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Definitely gonna make the inversion on some undersink RO system or something like that... Thanks for the info
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Re: Cultivation General Discussion [Re: gabbk]
#27290613 - 05/02/21 03:29 PM (2 years, 8 months ago) |
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About to get super loaded and spawn like 15 shoeboxes and a tub of PE. Wish me luck
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smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,862
Last seen: 13 days, 9 hours
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27290962 - 05/02/21 07:37 PM (2 years, 8 months ago) |
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Quote:
p9hu7 said:
Quote:
sandman420 said: any experienced grower wouldn't attempt to clean that culture unless that was the last culture on earth. We got better shit to do.
That plate would have been thrown out long ago. Make more than one plate if these are the possible results. This looks like a germ plate, I would suggest making more than one next time around.
You don't want a culture that has mold meshed into the mycelium, its a pain in the twat. Best scenario for this plate, if you absolutely must clean it is to use josex's puck method.
Edit* Here you go:
https://www.shroomery.org/forums/showflat.php/Number/24806569
Sorry, I must admit that I just looked at the picture and the part about saving it
Quote:
smalltalk_canceled said:
 this is my best growth from the single Ape swab I have ever had.
Can it be saved?
Best of luck.
I can't let go of the dream that saving this plate in ways contain the crux of what germination agar work is. What this hobby is about. Getting clean growth
When you are bad, new, you are tempted to try to save shit like this for multitude of reasonable reasons one being when you lack clean myc. Or have a bad syringe or swab. Happens all the time.
It's just somewhere during growing up with your agar work, you know transfers from sporulating mold wil almost always fail
Hence why posters rip it, saying it will mesh too
I got ten plates ready here, just took a shower. Regular SAB prep.
I'm going to use a technique I call the double transfer in hopes of getting one visually clean plate,
Time to rock
-------------------- Willpower is the one true virtue
  
Edited by smalltalk_canceled (05/02/21 07:40 PM)
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Its not about ripping on your culture or you, its about being reasonable. Mold absolutely will mesh into your culture, I've experienced it first hand.
Taking normal transfers from plates laden with mold spores is going to more than likely cause you many downstream headaches. We post responses to problems to help solve problems.
Good luck, hopefully it works. I would highly recommend cleaning this culture properly though, it is seriously fucked. I don't have a horse in the race so it ultimately doesn't matter what you do but I recommend that you use that method posted by josex, it will get you there with a greater chance of success.
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27290989 - 05/02/21 07:54 PM (2 years, 8 months ago) |
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I had originally misread your question smalltalk, you had asked if an experienced grower COULD save it. And they may or may not. It would need to be worked on in a SAB instead of a hood because like p9hu7 said it can cause downstream contams and you can't grab clean gorowth if you blow spores all over it. It's certainly possible that it could be done. Just wouldn't want to unless it was hellllllla special. It would probably tak e alot of work and you may never be able to get it clean after all that work. Intertwined contams become big big problem. Trich can become a mycoparasite.
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smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,862
Last seen: 13 days, 9 hours
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Back from the SAB, made 9 left one control
Will report result in a few days and I'm waiting
Yeah it's COULD not should. I have a long experience with it being difficult
-------------------- Willpower is the one true virtue
  
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smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,862
Last seen: 13 days, 9 hours
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I mean if people made good swabs you wouldn't need this but hey they don't
Never take swabs
-------------------- Willpower is the one true virtue
  
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Swabs are nearly always a little dirty to a lot dirty IME. They can really benefit from antibacterial agar.
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
Last seen: 1 year, 5 months
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Re: Cultivation General Discussion [Re: sandman420]
#27291029 - 05/02/21 08:12 PM (2 years, 8 months ago) |
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Quote:
sandman420 said: Trich can become a mycoparasite.
Is pretty much. It just hitchhikes and waits for its moment, then it fucks you over.
That's why all the advice saying "don't try and save that growth" because all around what you want is growing what you don't want.
Sure you MIGHT be able to get it looking clean, but will it REALLY be clean after all that work? The plate needs to be backed up to BEFORE the contams took hold, and then you just take it, in a SAB, from the cleanest spot possible. Unfortunately there isn't a rewind button so start again and grab the first myc that shows up. Much easier to start clean and stay clean then clean up afterwards.
Germinating cultures will always have plenty of genetic strains in it by the time you can actually see dikaryons on the plate...
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if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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Irishmule169
Hunter



Registered: 10/21/19
Posts: 212
Loc: McKenna’s house
Last seen: 1 year, 2 days
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Re: Cultivation General Discussion [Re: PrimalSoup]
#27291039 - 05/02/21 08:20 PM (2 years, 8 months ago) |
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What’s the best tek to cleaning up syringes for LC to grain ?? I’m doing boiling water drawing it up in the syringes multiple times not back emptying in the pot . Torching the syringes before drawing up the LC to inoculate the spawn ... feed back welcomed ..
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Wall.E
Bacteria's Bitch



Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
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Re: Cultivation General Discussion [Re: Irishmule169]
#27291057 - 05/02/21 08:29 PM (2 years, 8 months ago) |
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Just pc them. Wrap em with the needles on and bring to pressure for like 5 minutes, or just toss em in with your agar run. Still need to flame the needle, but beats the boiling water
-------------------- Life’s shit, but I’m loving it
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The Fresh Prints
Smell ya later



Registered: 05/19/12
Posts: 1,377
Loc: Bel-Air
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Quote:
smalltalk_canceled said: I mean if people made good swabs you wouldn't need this but hey they don't
Never take swabs
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: sandman420]
#27291086 - 05/02/21 08:44 PM (2 years, 8 months ago) |
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I give swabs a dunk in sterile water, swish it around for like ten seconds. If I suspect the swab is dirty I give it another dunk in a second container with sterile water and then do this:
Quote:
- First of all, prepare a shit ton of BRF pucks. A single swab can inoculate a ridiculous amount of pucks using this method.
- Use a little filtered container to sterilize enough water to cover the bottom half an inch.
- In the SAB, take the swab and dip it in the sterile water to soften up the cotton fibers and hopefully wash off any contamination. Don't over do it, 10 seconds is enough.
- Take your scalpel and use the tip of the blade to remove a tiny, spore-laden piece of cotton from the swab. Here's where you'll understand the main reason to wet the swab, taking pieces off of will be much easier.
- Bury the piece of cotton in the BRF puck.
You can leave the swab on a clean surface between inoculations, like a clean piece of folded foil.
I promise it only takes doing this once to know why I go through the trouble of even fucking around with PFtek substrate just to make these pucks. Beautiful, clean and organized growth from the get-go. I routinely make LC from germ plates, that's how much I trust this method.
These things go slower than agar, if any contamination should occur it will make itself seen early on, usually in the form of mold. But I very rarely have any contamination at all from this method.
The quote was taken from my tek (at the end): https://www.shroomery.org/forums/showflat.php/Number/24806569#24806569
Germ plates:

I wouldn't be surprised some of you could think I'm absolutely full of shit when I tell you almost every grow I've done from the '20 summer onwards has been from germ plates like these. I very rarely run clones, it's mostly MS grows from rad genetics, from fucking germ plates. That's how insultingly easy I make this hobby to be and it's weird that I'm posting this today because honest to god I keep a lot of this shit to myself because I'm such a competitive ass and it feels good to have my little secrets
Edited by Josex (05/02/21 09:17 PM)
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