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Wall.E
Bacteria's Bitch



Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
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Re: Cultivation General Discussion [Re: BrownBear]
#27245253 - 03/09/21 03:25 PM (2 years, 10 months ago) |
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Maybe my link has something that will help piece it together brownbear. It's me like fungal fucking 101, but still.
From my understanding a mushroom in itself is a plethora of mycelium but the spores are created from the dikaryon and this is what I'm confused about. Is 1 mushroom 1 dikaryon or is it a multitude of them?
You can take tissue to clone it but if you took transfers from each quadrant and put those to grain, would they all have the initial mushroom's traits or would there be variance? I'm pretty sure there's variance (idk haven't gotten that far in my grows yet) and you have to keep narrowing it down. So I'm imagining you're right about transfers but idk how many
-------------------- Life’s shit, but I’m loving it
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BondVillain
Stranger



Registered: 08/14/18
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Re: Cultivation General Discussion [Re: LtLurker]
#27245274 - 03/09/21 03:38 PM (2 years, 10 months ago) |
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Quote:
LtLurker said: I do pf tek all the time and use tall jars. I don't have a problem with a compacted bottom unless i did something like make it too wet or compress too much for the dry verm layer.
Turning upside down is worse. If enough condensation runs down, it can ruin the dry verm. Plus the disruption in the layer when you actually flip it.
I'm trying to trouble-shoot my jars 'pinning' prematurely. Does this happen to you? How do you prevent?
I was thinking keeping the jars in a dark closet with no light exposure. Last time I think I was fussing too much and moved the jars from one location to another location that had too much light.
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LtLurker
Lost Sailor



Registered: 01/03/18
Posts: 7,535
Loc: Borderlands
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Re: Cultivation General Discussion [Re: BondVillain] 1
#27245289 - 03/09/21 03:45 PM (2 years, 10 months ago) |
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occasionally. Usually if it's pinning before 100% it's usually linked to some bacteria or other contam happening as far as i can tell. I use "double printing" like in bod's spore printing tek to get as clean a print as possible and don't see much premature pinning from my own prints.
No light will not help you. Light is beneficial at all stages and mushrooms will still attempt growing in the dark if they have to.
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Lenz
Misunderestimated


Registered: 08/17/20
Posts: 692
Loc: Fungistan
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Re: Cultivation General Discussion [Re: BondVillain]
#27245295 - 03/09/21 03:53 PM (2 years, 10 months ago) |
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I've been wondering the same thing brownbear. Didn't see you were talking about this here before I commented in the photo thread but yeah I've basically been wondering how stable a clone culture exactly is.
I guess it depends on the clone and how much it sectors as well but do you basically have to take a clone to an isolate to be able to run it a bunch of times with minimal variance? Cause I'd think that after a decent amount of transfers the genetic pool will be smaller and potentially fruit differently.
I found these 2 threads helpful: https://www.shroomery.org/forums/showflat.php?Cat=&Board=2&Number=23645444&fpart=&PHPSESSID= https://www.shroomery.org/forums/showflat.php?Cat=&Board=4&Number=22807226&fpart=&PHPSESSID=
There's also this in the 2nd thread:
Quote:
Inocuole said: The fact that you can isolate further past the point of a clone says everything about their consistency. They are more consistent than from sopres by a landslide, but an isolate is yet even more consistent since it only has one set of genetics. Unlike cannabis clones, there is more than one set of genetics at play in the average mushroom clone.
In the same vein, a clone could offer more differing reactions to environmental conditions than an isolate might, since it could plausibly have a set of genetics within, where each respond to different conditions separately.
Sometimes a clone is better, sometimes an isolate is better, but one is significantly easier to get a hold of, so most work with clones.
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TedsDead



Registered: 01/03/17
Posts: 4,998
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Re: Cultivation General Discussion [Re: BrownBear]
#27245330 - 03/09/21 04:20 PM (2 years, 10 months ago) |
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Quote:
Iambrownbear said: So you have had better results with ms than you have had with clones?
I guess the real motivation behind my question is I am not sure how much credit I can actually give myself for the canopies that I grow. I was given clones that were from various transfers. I have made various transfers myself before growing them out. Do I credit my results to the one who did the work with the clones or have I created the perfect fruiting environment for these cultures to grow? The only ms culture that I have grown to date so far are toc but they are known to be prolific fruiters from ms.
Don't get me wrong. I appreciate this person and what they have done for me and I have mad respect for them. However, my goal is to become the best cultivator that I can be. Good news is I have a few ms cultures on grain right now so I should know soon enough.
Its a combination of the 2. You cant push a culture past what the genetics give you. You want strong genetics coupled with the best conditions for said genetics. Even tho you have a clone from someone else you know how to provide it with what it wants so that it really shines. Ive gotten better results from good clones than from ms any day but there are also shitty clones. Good ms genes and bad ms genes
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
Edited by TedsDead (03/09/21 04:21 PM)
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BrownBear
Warrior-Traveler



Registered: 06/05/20
Posts: 1,539
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Re: Cultivation General Discussion [Re: Lenz] 1
#27245332 - 03/09/21 04:23 PM (2 years, 10 months ago) |
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Thanks. Those links provide some good reading. Going to have to dive into them when I have a few minutes.
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BrownBear
Warrior-Traveler



Registered: 06/05/20
Posts: 1,539
Last seen: 8 months, 7 days
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Re: Cultivation General Discussion [Re: BrownBear]
#27245336 - 03/09/21 04:29 PM (2 years, 10 months ago) |
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Thanks, ted. Honestly I feel like I am not doing anything special. I definitely don't want to take credit for something that someone else put the work in for. I know my line of questioning may seem like I am trying to do the opposite but I just want to use deductive reasoning to come to a conclusion. You and Aka make alot of sense though in that it is a combination of both genetics and environmental parameters.
Edited by BrownBear (03/09/21 04:30 PM)
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TedsDead



Registered: 01/03/17
Posts: 4,998
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Re: Cultivation General Discussion [Re: BrownBear]
#27245359 - 03/09/21 04:47 PM (2 years, 10 months ago) |
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Learning how to regognize what the culture wants and give it to it is a big part of the game. #Observations. This last run of pbr that I did I cut my fans in hopes of larger fruits. I usually favor max FAE and misting/evaporation but this approach was giving me stubbies. It worked. Pinsets werent as impressive but fruit size increased quite a bit. It evened out in the end yield wise. Other strains that I have like the other approach tho. Just gotta decide what gives you what you want
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,820
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Re: Cultivation General Discussion [Re: TedsDead]
#27245539 - 03/09/21 07:04 PM (2 years, 10 months ago) |
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do any of you pick caps that have opened but leave the stipes attached to the sub to let the rest of a flush finish? i figured it's fine to do but was just curious. i have half a tub starting to drop spores & the other half as pin clusters just waiting to burst up. didn't wanna disturb the sub yet as some i'd have to harvest are from side pins that made it up the side of the tub
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
  🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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A.k.a
Stranger



Registered: 10/27/19
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Loc: Gaming the system
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Re: Cultivation General Discussion [Re: hazyhorse]
#27245579 - 03/09/21 07:37 PM (2 years, 10 months ago) |
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Yep. Mostly with clusters where one finishes way before the others stuck to it.
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LAGM2020     
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TedsDead



Registered: 01/03/17
Posts: 4,998
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Re: Cultivation General Discussion [Re: A.k.a]
#27245664 - 03/09/21 08:36 PM (2 years, 10 months ago) |
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in that case I either pluck out the finished fruits or just do an early harvest on the whole tub. you'll be fine regardless
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,820
Last seen: 1 day, 17 hours
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Re: Cultivation General Discussion [Re: TedsDead]
#27245832 - 03/09/21 10:54 PM (2 years, 10 months ago) |
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thx guys!
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
  🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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Mr. Mushie

Registered: 01/16/20
Posts: 667
Last seen: 2 months, 25 days
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Re: Cultivation General Discussion [Re: hazyhorse]
#27246248 - 03/10/21 08:04 AM (2 years, 10 months ago) |
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I had some mushrooms in the fridge overnight waiting for the dehydrator to free up.
I guess I had the fridge temp a little too cold because a few of them felt almost frozen while others were totally fine.
Should I toss those? They're in the dehydrator now and look fine but was curious what you all think.
Not sure if them partially freezing has any real negative effects.
Probably not because some people freeze dry them? It's only mycelium on agar and grains that we try to keep from freezing because that can kill the culture, yeah?
Edited by Mr. Mushie (03/10/21 08:05 AM)
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Camera93
We got dicks like Jesus



Registered: 08/15/18
Posts: 3,224
Last seen: 9 hours, 27 minutes
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Re: Cultivation General Discussion [Re: Mr. Mushie]
#27246251 - 03/10/21 08:08 AM (2 years, 10 months ago) |
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I believe the idea is that freezing fresh fruit will freeze the water in them. And once they thaw, they turn to goo
-------------------- All I need are some tasty waves, a cool buzz, and I’m fine. Whatever you decide won’t really impact our survival Close your eyes, and do the best that you can
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
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Re: Cultivation General Discussion [Re: Camera93]
#27246254 - 03/10/21 08:10 AM (2 years, 10 months ago) |
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Yeah the water expands when it freezes which ruptures the cells and they turn to goo when they thaw. I’d just thaw them out and see what happens.
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LAGM2020     
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Dabadava
Za-gor-te-nay
Registered: 02/22/21
Posts: 20
Loc: Croatia
Last seen: 1 year, 4 days
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Re: Cultivation General Discussion [Re: Wall.E]
#27246381 - 03/10/21 09:40 AM (2 years, 10 months ago) |
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At my place I can't find a cheaper hepa filter than 250 EUR, so I thought to buy two of this: https://www.ebay.com/itm/HOT-Formaldehyde-Bacterial-PM0-3-HEPA-Filter-For-Xiaomi-Mijia-Air-Purifier-MAX/193899455244?hash=item2d254ee30c:g:B~AAAOSw3Y1gLgtK and combine them to make 480*660*56cm flow hood. Any suggestions?
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: Feasoghorm]
#27246650 - 03/10/21 12:20 PM (2 years, 10 months ago) |
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Quote:
Feasoghorm said: BrownBear - That's why i dnt fuck with clones too much, or why i prefer to take the least amount of transfers possible from spore. Genetic ocean is where it's at. Every clone ive run was mega fucked in many ways.
MS is super reliable for yield, but I've had potency complaints with MS that I didn't have with clones. That's why I prefer finding a good clone. Obviously YRMV and you should do whatever works for you.
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Vinci
I'm You



Registered: 08/26/19
Posts: 629
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Bear my sig shoutout was for general discussion, not to answer you
-------------------- Vinci loves you
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clockworkshroom
Stranger


Registered: 02/20/20
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Re: Cultivation General Discussion [Re: Vinci]
#27246797 - 03/10/21 02:08 PM (2 years, 10 months ago) |
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On the topic of canopies check out my latest...

I'll get there one day
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 10 hours, 13 minutes
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Did you mist that tub a lot?
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