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OfflineNattyBoh
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Registered: 08/17/16
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Last seen: 5 years, 30 days
Re: Cultivation General Discussion [Re: Schemenhaft]
    #24097322 - 02/17/17 09:26 AM (6 years, 11 months ago)

I have been experimenting with LC, knocking up popcorn. I've been very successful thus far. Cambodians, in pint jars 1week fully colonized. I'm always scared the LC might be contaminated. I'm considering, doing cardboard cloning tek. Inoculating with my LC. Would I be able to tell if it is viable. Maybe just transfer cardboard to my grain? Of course in sterile conditions. I'm not sure if this makes sense, or a waste of time?


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InvisibleComebackKid
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Re: Cultivation General Discussion [Re: NattyBoh]
    #24097503 - 02/17/17 10:45 AM (6 years, 11 months ago)

You can check your LC by putting a drop to agar. If bacteria shows up then you know it's contaminated

Not sure what this cardboard cloning tek is but it doesn't sound very good


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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InvisibleMr Piggy
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Re: Cultivation General Discussion [Re: NattyBoh]
    #24097597 - 02/17/17 11:21 AM (6 years, 11 months ago)

Quote:

NattyBoh said:
I have been experimenting with LC, knocking up popcorn. I've been very successful thus far. Cambodians, in pint jars 1week fully colonized. I'm always scared the LC might be contaminated. I'm considering, doing cardboard cloning tek. Inoculating with my LC. Would I be able to tell if it is viable. Maybe just transfer cardboard to my grain? Of course in sterile conditions. I'm not sure if this makes sense, or a waste of time?





Wait, hold up.  Are you doing stembutt transfers to cardboard then trying to use that in LC?

If you're not cleaning up on agar you're going to have dirty LC.


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🅃🄴🄰🄼 🄵🄾🄸🄻


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OfflineNattyBoh
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Re: Cultivation General Discussion [Re: Mr Piggy]
    #24097628 - 02/17/17 11:33 AM (6 years, 11 months ago)

I was considering sterilizing a small piece of corrugated cardboard in a small 1/4pint jar with self sealing ports. Introduce a cc of my LC, let it colonize, then do a transfer to my grain jars. U figured if mycelium grew it was contam free? I am definitely going to research agar. Just my ignorance on agar intimidates me.


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: NattyBoh]
    #24097633 - 02/17/17 11:35 AM (6 years, 11 months ago)

LC without agar is near impossible.


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InvisibleMr Piggy
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Re: Cultivation General Discussion [Re: NattyBoh] * 1
    #24097642 - 02/17/17 11:38 AM (6 years, 11 months ago)

Quote:

NattyBoh said:
I was considering sterilizing a small piece of corrugated cardboard in a small 1/4pint jar with self sealing ports. Introduce a cc of my LC, let it colonize, then do a transfer to my grain jars. U figured if mycelium grew it was contam free? I am definitely going to research agar. Just my ignorance on agar intimidates me.




If you already have a clean LC it should go directly to grain, but you do not have a clean LC if it's from spores.  Don't add a step where one isn't needed.

You're going to have to get into agar if you want to do this hobby.  It's not difficult at all.  If you can make brownies or mac n cheese by following the directions on a box, you can do agar.


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🅃🄴🄰🄼 🄵🄾🄸🄻


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OfflineNattyBoh
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Re: Cultivation General Discussion [Re: Mr Piggy]
    #24097665 - 02/17/17 11:45 AM (6 years, 11 months ago)

Lol thanks , thank god I'm only 3/4's stupid


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InvisibleMr Piggy
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Re: Cultivation General Discussion [Re: NattyBoh]
    #24097670 - 02/17/17 11:47 AM (6 years, 11 months ago)

Got me beat, I'm half smart :frown:


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Offlinetombosley8
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Re: Cultivation General Discussion [Re: tombosley8]
    #24097872 - 02/17/17 01:16 PM (6 years, 11 months ago)

nice thanks noob I think I'm going with the 3'x4' filter http://www.globalindustrial.com/p/hvac/filters/airex-filters/airex-174-36-x48-x6-hepa-filter-micro-air-99-97-efficiency-wood-frame-high-quality

as long as this blower https://www.grainger.com/product/DAYTON-3-4HP-115V-Double-Inlet-Forward-1XJY2?s_pp=false&picUrl=//static.grainger.com/rp/s/is/image/Grainger/1XJY2_AS01?$smthumb$ is sufficient.

Having trouble figuring out how much power the blower needs for this large of a filter and if the filter will deliver laminar flow when around 1.2" of pressure.

Do I need the specs for the filter to be sure they will work together or with the info I have provided would they be a good match?


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OfflineTiamo
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Re: Cultivation General Discussion [Re: tombosley8]
    #24097882 - 02/17/17 01:20 PM (6 years, 11 months ago)

I've read multiple times on these forums that spores are supposed to taste like shit. I got real curious and saw that fresh mushrooms I just picked had left a nice big spore print on my table. I put my finger to the spores and gathered a generous amount of them and put them in my mouth... they taste like nothing. Absolutely nothing. I can't discern any taste, whatsoever. Why do people continue to spread this "fact"?


--------------------


If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:


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InvisiblemagicMerlin
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Re: Cultivation General Discussion [Re: NattyBoh]
    #24097944 - 02/17/17 01:45 PM (6 years, 11 months ago)

Quote:

NattyBoh said: I am definitely going to research agar. Just my ignorance on agar intimidates me.




Give this a read. If the materials intimidate you, there are simpler ways of making agar plates.


--------------------
My favorite threads



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OfflineNattyBoh
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Re: Cultivation General Discussion [Re: magicMerlin]
    #24098140 - 02/17/17 03:13 PM (6 years, 11 months ago)

I did start my LC with a spore syringe, I saved 1cc from a Cambodian. I used it for brf cakes 2 successful results. I then tried popcorn did a small bucket grow spawned to coir verm, and was rewarded. I'm presently putting my 1st monotub out to fruit now. Did I just get lucky with my spore syringe to LC? Or my tub doomed to fail? Gosh now I'm worried :-0


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OfflineAlCapone2k
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Re: Cultivation General Discussion [Re: NattyBoh]
    #24098272 - 02/17/17 04:27 PM (6 years, 11 months ago)

Pf-Tek uses BRF which is more contamn resistant than grains. That is the reason why PF-Tek works with contaminated spore syringes while bulk will fail.

Which does not mean, that your bulk will fail, you can be very lucky to have got a very clean spore syringe which allows you to get some shrooms with your bulk tek :smile:


Regards


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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InvisibleComebackKid
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Re: Cultivation General Discussion [Re: AlCapone2k]
    #24098428 - 02/17/17 05:38 PM (6 years, 11 months ago)

LC with from spores is asking for trouble. It can work. If you're lucky


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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InvisibleMoabfighter
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Posts: 2,710
Re: Cultivation General Discussion [Re: ComebackKid]
    #24098519 - 02/17/17 06:25 PM (6 years, 11 months ago)

Just did 4 PE clone transfers, 1 Texas Yellow Cap clone transfer, and a 7th gen KSSS transfer to keep it alive...

Should the TYC be good to inoculate with this plate, it's first transfer off an agar pin.


--------------------
KSSS And PE WBS.
 


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InvisibleMunchauzen
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Re: Cultivation General Discussion [Re: Moabfighter]
    #24098523 - 02/17/17 06:29 PM (6 years, 11 months ago)

texan strains have some of the very best MS genetics


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InvisibleJosex
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Re: Cultivation General Discussion [Re: Munchauzen]
    #24098536 - 02/17/17 06:38 PM (6 years, 11 months ago)

Good to know! I have 2 tubs and some shoeboxes that look like they'll pin soon but it is Texas Orange Cap that I got from Greg. I'd like to know the history behind that strain, anyone?


Edited by Josex (02/17/17 06:44 PM)


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OfflineAlCapone2k
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Re: Cultivation General Discussion [Re: Munchauzen]
    #24098545 - 02/17/17 06:44 PM (6 years, 11 months ago)

Hey guys,

some question to the genetic pros around here :smile:

Is it possible to make a selection of a mushroom/strain which produces spores that germinate and build mycelium, but this mycelium does not fruit?

The reason for my question: I have made several spore prints some months ago of a B+-Mushroom I got from a Kit bought in the Netherlands.

As you may know, the netherlands are the only country in europe where shrooms are legal so they have a quite big industry with grow kits, spores, et cetera.

Eventhough I did about 10 plates with this spore prints (at least three different ones) and all of them germinate to myc, non of the plate has every gave me fruits (plate -> rye -> bulk).

The last bulk I tried with one of these plates took 6 weeks and not even formed any knots.


Since all my other bulks worked fine and gave me a lot of fruits, the only reason I can imaginge for that non-fruiting myc is that the dutch guys just selected a B+-strain which does produce spores but the spores don't germinate to fruiting myc. The reason could be to force guys to buy spores from them instead of making own spore prints out of the kit shrooms, you know what I mean?


Could that be possible?


(Just so say: It also could be that all my spore prints and over 10 ms plates I made of them had all bad non-fruiting genetics, but it seems very unprobable).


Would like to hear your opinions.


Regards


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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InvisibleLoveshroomz777


Registered: 11/19/16
Posts: 616
Re: Cultivation General Discussion [Re: AlCapone2k]
    #24098565 - 02/17/17 06:50 PM (6 years, 11 months ago)

I am also growing Texas orange cap just waiting for the jars to finish colonizing can't wait to see how they turn out as well as try them:chesire:


--------------------
PEACE, LOVE AND PSYCHEDELICS



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OfflineKenetic
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Re: Cultivation General Discussion [Re: AlCapone2k]
    #24098570 - 02/17/17 06:52 PM (6 years, 11 months ago)

More likely the conditions of the plate.  I've never seen a pin on my plates, I still have a few that are almost 6 months old


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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