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OfflineGypsyBastard
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Re: Cultivation General Discussion [Re: smalltalk_canceled]
    #27241936 - 03/07/21 01:30 PM (2 years, 10 months ago)

Smalltalk, I would focus on your sterile technique as a vector. Contam seems to start at the top and move down suggesting it's being introduced when adding the agar, but not from the agar.
:2cents:

Watch your movements, and air currents (if using SAB). Flame all tools often. All that jazz. Maybe try flaming higher up the handle of your scalpel or tongs. I know having to tap the handle to get the wedge off the blade caused problems for me early on and flaming higher up and more frequent iso wipes helped with that.

Also, control jar. If the control contams without every being opened then the issue is with the seal\filter on the jar.


Edited by GypsyBastard (03/07/21 01:37 PM)


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Offlinesmalltalk_canceled
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Re: Cultivation General Discussion [Re: GypsyBastard]
    #27241940 - 03/07/21 01:35 PM (2 years, 10 months ago)

Thing is, I make squeaky clean agar myc,I'm only experiencing this with *some* grain jars batches.

I'm trying to isolate where this is going wrong. I can't assume it's the inoculate before I rule out pc cycle, can I?

I have 4 different pressure cookers, so any of them could be faulty



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Willpower is the one true virtue



Edited by smalltalk_canceled (03/07/21 01:36 PM)


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OfflineGypsyBastard
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Re: Cultivation General Discussion [Re: smalltalk_canceled]
    #27241944 - 03/07/21 01:38 PM (2 years, 10 months ago)

I personally would suspect the jars before the PC unless every jar in a run is developing the same contam at the same time.


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Offlinesmalltalk_canceled
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Re: Cultivation General Discussion [Re: GypsyBastard]
    #27241952 - 03/07/21 01:47 PM (2 years, 10 months ago)

Quote:

GypsyBastard said:
I personally would suspect the jars before the PC unless every jar in a run is developing the same contam at the same time.




That's exactly what I'm experiencing tho

That jar was part of 9 jars all going green the same way, all inoculated 1st of march. Same PC, same inoculate.

But it could still be the inoculate spawn, and you are right in my book for mentioning it.

If the control jars mold, I'll have one answer but I figure the collective experience of Shroomery have more methods than the control for indicating incomplete pc cycle.

Rules of thumb etc


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Willpower is the one true virtue



Edited by smalltalk_canceled (03/07/21 01:48 PM)


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OfflineGypsyBastard
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Re: Cultivation General Discussion [Re: smalltalk_canceled]
    #27241961 - 03/07/21 01:52 PM (2 years, 10 months ago)

I really don't think it's your inoculant, rather your process, if you say the contams don't appear to be coming from the agar.
My question about the jars in the same cycle that all went bad did the contams in the jar start evenly throughout the jar, or did they start at the top and work their way down, or at the bottom and work their way up?

Just to be clear though, I am entirely a novice and not an expert or authority on this. I just enjoy troubleshooting so I'll lend you my brain if it'll help.

if the contam seem to be starting throughout the jar fairly evenly, that would point towards the PC cycle not being sufficient if the contams seem to be starting at one end or other of the jar and then moving across (or up\down), that would suggest can Tam's getting into the jar after sterilization.


Edited by GypsyBastard (03/07/21 01:55 PM)


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InvisibleFeasoghorm

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Re: Cultivation General Discussion [Re: smalltalk_canceled]
    #27241967 - 03/07/21 01:54 PM (2 years, 10 months ago)

I think that pressure cookers don't normally come with thermometers cuz it aint necessary. 200° hot air or 200° steam...still 200°.

Why the fuck do people keep using strait corn? There is a reason experienced members of the OMC advise against it.

And im not proud of this by any means but feel i must mention that i had mega contam issues when transferring tissue to grain cuz i wasnt wearing gloves. We shed skin all the time and them skin cells carry shit. May be an issue for others as haphazard as i was.


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Offlinesmalltalk_canceled
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Re: Cultivation General Discussion [Re: GypsyBastard]
    #27241970 - 03/07/21 01:56 PM (2 years, 10 months ago)

Kinda looks like the bottoms are the cleanest thing on all of them, definitely a good suggestion. I'll post a SAB video if I keep having problems


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OfflineGypsyBastard
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #27241971 - 03/07/21 01:57 PM (2 years, 10 months ago)

I was super lazy on my last sab session and totally did not wear gloves I was aware it was a stupid move so I was even more cautious than normal, even though I am surprised that none of the jars showed contams. doesn't mean that they're not, just means there's probably some hidden bacterial in there

Quote:

smalltalk_canceled said:
Kinda looks like the bottoms are the cleanest thing on all of them, definitely a good suggestion. I'll post a SAB video if I keep having problems




If it's every jar, and contams appear to start at the top my first step would be checking the seals on the filters in the jarl did. I had some lids that I had silicone sfds onto, and a few of the silicone layers had holes in them allowing some tams to get in around the filter. If using poly, it may not be tight enough. If using MP tape, may just need more layers. if using no filters and just cracking the lid, it may be that the jars do not have enough threads to create an obstructed pathway, allowing contams to flow in. 


Edited by GypsyBastard (03/07/21 02:02 PM)


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Offlinetedoro
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #27241981 - 03/07/21 02:07 PM (2 years, 10 months ago)

Quote:

Feasoghorm said:
I think that pressure cookers don't normally come with thermometers cuz it aint necessary. 200° hot air or 200° steam...still 200°.





15psi with all steam is 250f. 15psi with air is like 220f or something. You are wrong.


--------------------
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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


Edited by tedoro (03/07/21 02:08 PM)


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OfflineGypsyBastard
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Re: Cultivation General Discussion [Re: tedoro]
    #27241984 - 03/07/21 02:13 PM (2 years, 10 months ago)

Well, he's right and wrong.
200° of steam can't exist because steam doesn't exist until 212. However 250 of hot air and 250 of steam are both 250. The difference is, the psi.
That's where the thermometer comes in. if the PSI is 15 and a thermometer is less than 250, you have air in it. it's kind of like a weird fire triangle kind of thing. You need two points of reference to assure the third.
with all that being said, I do not have a thermometer on my pc. I vented for 10 minutes, and I trust the gauge and the rocker. I know the sound of it when the gauge reads 16-17psi and have no reason at this point to doubt the accuracy of my gauge. You are not wrong though that using a thermometer in combination with a gauge does give you more of a confirmation that what you think is going on inside is actually going on inside


Edited by GypsyBastard (03/07/21 02:19 PM)


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Offlinetedoro
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Re: Cultivation General Discussion [Re: GypsyBastard]
    #27241993 - 03/07/21 02:16 PM (2 years, 10 months ago)

Tellin you.. adding a thermometer is the shit. It means I can set an alarm for any pressure I want, with a 7.99 amazon meter. No more hearing the bobble go wild and running up to the stove with your face turned away as turn frantically turned the stove down. We've all been there. Mine beeps as it approaches 14psi.


--------------------
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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: tedoro]
    #27241999 - 03/07/21 02:20 PM (2 years, 10 months ago)

I prefer a dialed in stove and counter weight.

My stoves lowest burner setting + 60cents taped to rocker = silent operating all day with damn near zero water loss.


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Offlinetedoro
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Re: Cultivation General Discussion [Re: mushboy]
    #27242005 - 03/07/21 02:23 PM (2 years, 10 months ago)

so Mush, are you one of those folks that only has the stove on one setting, and just let it take forever to get up to pressure? I heard of that...


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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OfflineGypsyBastard
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Re: Cultivation General Discussion [Re: mushboy]
    #27242011 - 03/07/21 02:28 PM (2 years, 10 months ago)

I can definitely see it's application and I dig the ingenuity in your solution. Most of my pc runs are done outside on a propane burner though and honestly I wish I had started doing that sooner. I can control the pressure so much more precisely than I ever could on my stove top, come up is faster, PSI more constant, and it doesn't turn my kitchen into a sauna.
But without some sort of fancy Bluetooth connection or a very loud alarm, there's no way a thermometer on a PC in my detached garage is going to alert me of anything if I'm in the house. The biggest problem I've run into is me forgetting it was venting and it running out of water 11 minutes before the 120min cycle was done but that actually didn't cause any issues and could just as easily be solved with a 10 minute timer on my phone or me not being dumb. Timer is more likely.

I also just wanted to add, I know this discussion came up because somebody was asking how to double check themselves to make sure things are going as they think they are. In that discussion, you are 100% correct. Adding another critical control point is never going to not give you more accurate understandings of the process. I don't want this to come off as bashing or dismissing your idea


Edited by GypsyBastard (03/07/21 02:31 PM)


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Offlinetedoro
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Re: Cultivation General Discussion [Re: GypsyBastard]
    #27242014 - 03/07/21 02:31 PM (2 years, 10 months ago)

They have some cool ass remote thermometers for BBQ masters who want to wander. Swap one of those for the simple one I use. They have an app.


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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OfflineGypsyBastard
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Re: Cultivation General Discussion [Re: tedoro]
    #27242017 - 03/07/21 02:35 PM (2 years, 10 months ago)

I despise apps and anything interconnected. The Internet of things is a terrible thing. if I really wanted to, I could just set one of my hardwired security cameras looking at the thermometer display and watch it from inside the house but I just don't see the need in my case. It's just overly complicated. I can set a 10 minute timer on my phone walk inside and walk back out when it's done. I have my regulator marked so I know exactly where to turn it and it will hold the PSI right where it should. when I step outside to smoke I might double check it and make sure nothing's changed, but for the most part I can just set up two hour timer on my phone and forget about it until the timer goes off.
I just don't need it. It's just extra steps and extra expenses.

With that said, if I ever do find myself questioning the quality and adequacy of my PC runs, this little tidbit is stored in the back of my head and will be my first go to to double check that at 15 psi it is at the temp it should be. Just not for a regular use kind of thing


Edited by GypsyBastard (03/07/21 02:41 PM)


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Offlinetedoro
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Re: Cultivation General Discussion [Re: GypsyBastard]
    #27242024 - 03/07/21 02:42 PM (2 years, 10 months ago)

Dude, I get it. KISS.

It did once start beeping at me when I forgot to add water. That was nice. Bet I'm not the only one that has done that either.


--------------------
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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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OfflineEugene Gesuale
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Re: Cultivation General Discussion [Re: tedoro]
    #27242038 - 03/07/21 02:57 PM (2 years, 10 months ago)

What happens if I decide to leave bottom pins there without harvesting. I just don’t want to bother


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: tedoro]
    #27242051 - 03/07/21 03:08 PM (2 years, 10 months ago)

Quote:

tedoro said:
so Mush, are you one of those folks that only has the stove on one setting, and just let it take forever to get up to pressure? I heard of that...




Nah I crank it up to full to vent and after cleaning up grain prep, smoking a bowl and rearranging my balls(roughly 15-20min) I back it off to low and set the microwave alarm for 120min.


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: Eugene Gesuale]
    #27242052 - 03/07/21 03:09 PM (2 years, 10 months ago)

Quote:

Adrenochrome said:
What happens if I decide to leave bottom pins there without harvesting. I just don’t want to bother





Sometimes they can get contamination and rott or theyll fuzz over and revert to myc.

Oops ment to edit not dbl post sry


Edited by mushboy (03/07/21 03:10 PM)


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