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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #27215084 - 02/19/21 03:22 PM (2 years, 11 months ago)

Quote:

ModularMind said:
The official unpopular opinion thread..
https://www.shroomery.org/forums/showflat.php/Number/27121993#27121993
:cookiemonster:



Lol ahh man that made me laugh. I needed that, Thnx
Faht - Indeed, motions towards keeping innocent life out of harms way do seem good.

Alright, well ive always wanted to try syringe to grain without a pc.
I wanna steam sterilize 2 or 3 qts of mine special mix grain in my deep soup pot for 3 hrs at a time like i did with 32 cakes on me first run.
Any suggestions on taking that route effectively? Like, are injection ports the shit for this method?

Quote:

p9hu7 said:
I love canna coir, it's smoother than an angels labia.



Jesus...you make it sound so enticing


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OfflineSankhara
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Registered: 02/11/18
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #27216013 - 02/20/21 04:11 AM (2 years, 11 months ago)

Guys, does any of you have a video for tiger dropping a whole petri dish (not pastiplate)?

I cant seem to get the idea for a proper technique, i couldnt find a video either


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Invisiblestareatclouds
star eat clouds?
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Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: Sankhara]
    #27216016 - 02/20/21 04:14 AM (2 years, 11 months ago)

I don't see how it's possible to do that with a regular Petri dish.


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Offlineroarkell
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Re: Cultivation General Discussion [Re: stareatclouds]
    #27216048 - 02/20/21 04:37 AM (2 years, 11 months ago)

Its possible... I do it all the time. You can't just squeeze the sides like a no pour but once you get the hang of it, I can drop a whole plate in 2 seconds or less...


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OfflineSankhara
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Re: Cultivation General Discussion [Re: roarkell]
    #27216057 - 02/20/21 04:47 AM (2 years, 11 months ago)

So... Whats your technique roar?  Do you tik tac toe the petri or slice the petri in 4 and then what?


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InvisibleLenz
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Registered: 08/17/20
Posts: 692
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Re: Cultivation General Discussion [Re: Sankhara]
    #27216061 - 02/20/21 04:50 AM (2 years, 11 months ago)

Would like to know this as well. When I do LI I have to cut the plate in 2 and then carefully carry both pieces one at a time to drop them into the sterile water but I've been trying to think of a better way where I can just do it in one motion. Not sure how to do it without a 3rd hand.


--------------------
:chems:


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Invisiblestareatclouds
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Re: Cultivation General Discussion [Re: Lenz]
    #27216067 - 02/20/21 04:55 AM (2 years, 11 months ago)

If you're cutting the wedge, it's not really a Tiger Drop, IMO. Definitely curious how you do that, roarkell. Are y'all referring to Pasty's 2.0 version where he makes slices first? Original TD is entire puck without using a blade, which was the appeal.


Quote:

Lenz said:
Would like to know this as well. When I do LI I have to cut the plate in 2 and then carefully carry both pieces one at a time to drop them into the sterile water but I've been trying to think of a better way where I can just do it in one motion. Not sure how to do it without a 3rd hand.




This is how I've done it and never had an issue. If both wedges are going to the same place, I just stab them both with the blade and do it in 1 trip.


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Offlineroarkell
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Re: Cultivation General Discussion [Re: Sankhara]
    #27216083 - 02/20/21 05:14 AM (2 years, 11 months ago)

Quote:

Sankhara said:
So... Whats your technique roar?  Do you tik tac toe the petri or slice the petri in 4 and then what?




I might try to figure out a way to make a video or maybe a step by step with pictures... im supposed to be getting up and starting grain anyway... lol.

Basically, I tic tax toe the plate but then I'll use the back side of the blade to ride along the bottom of the plate and make a quick "s" motion and just kinda scoop em all out... only thing that will fuck you up is if you let your plates get over grown (like I often do)... then you gotta walk a fine line along the edge pieces or the mycelium thats reached the petri wall will hold on and Tarzan swing outta that plate and zing right past the jar to the floor... very frustrating... lol


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OfflineSankhara
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Re: Cultivation General Discussion [Re: roarkell]
    #27216110 - 02/20/21 06:02 AM (2 years, 11 months ago)

Quote:

stareatclouds said:
If you're cutting the wedge, it's not really a Tiger Drop, IMO.




Yeah i totally agree, I may have used the wrong terminology i was just referring to techniques that use and entire plate to inoculate gran.


Quote:

stareatclouds said:


This is how I've done it and never had an issue. If both wedges are going to the same place, I just stab them both with the blade and do it in 1 trip.



Yeah, thats what i was kinda picturing i just thought  the wedges could easily break and fall during transfers.


Quote:

roarkell said:
I might try to figure out a way to make a video or maybe a step by step with pictures... im supposed to be getting up and starting grain anyway... lol.

Basically, I tic tax toe the plate but then I'll use the back side of the blade to ride along the bottom of the plate and make a quick "s" motion and just kinda scoop em all out... only thing that will fuck you up is if you let your plates get over grown (like I often do)... then you gotta walk a fine line along the edge pieces or the mycelium thats reached the petri wall will hold on and Tarzan swing outta that plate and zing right past the jar to the floor... very frustrating... lol



A video would be nice!

So basicale tic tac toe, run the blade to free them from the petri and then dump right into the jar?

That dumping is what worries me the most, I would have to put my entire hand and plate above the opening of the jar... That would make me really unconfortable. Do you use a SAB or hood?


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Invisiblestareatclouds
star eat clouds?
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Re: Cultivation General Discussion [Re: Sankhara]
    #27216157 - 02/20/21 06:59 AM (2 years, 11 months ago)

The wedges can break, but once you get a good feel for it, it's easy to get it done. You can also stab through multiple tic-tac-toe wedges with the blade, too. Making your agar harder and thicker (pause) will help with this. Also, 1/4 of a regular-sized Petri is more than enough for a 100ml LI. Perhaps you know that, though.

Is there any reason you don't want to use PP's for this? It's so much easier.


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OfflinePhony Phone

Registered: 09/19/20
Posts: 612
Last seen: 6 days, 18 hours
Re: Cultivation General Discussion [Re: stareatclouds]
    #27216206 - 02/20/21 07:43 AM (2 years, 11 months ago)

Guys, help me out here please.
There was a tek where you employ small amounts of spawn to germinate spores suspended in liquid syringe. I can't seem to find it.
If anyone knows what I am talking about, please link it here
thanks


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OfflineTweeq
Tweeq of Nature
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Registered: 06/07/18
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Re: Cultivation General Discussion [Re: Sankhara]
    #27216250 - 02/20/21 08:14 AM (2 years, 11 months ago)

Quote:

Sankhara said:
Quote:

stareatclouds said:
If you're cutting the wedge, it's not really a Tiger Drop, IMO.




Yeah i totally agree, I may have used the wrong terminology i was just referring to techniques that use and entire plate to inoculate gran.


Quote:

stareatclouds said:


This is how I've done it and never had an issue. If both wedges are going to the same place, I just stab them both with the blade and do it in 1 trip.



Yeah, thats what i was kinda picturing i just thought  the wedges could easily break and fall during transfers.


Quote:

roarkell said:
I might try to figure out a way to make a video or maybe a step by step with pictures... im supposed to be getting up and starting grain anyway... lol.

Basically, I tic tax toe the plate but then I'll use the back side of the blade to ride along the bottom of the plate and make a quick "s" motion and just kinda scoop em all out... only thing that will fuck you up is if you let your plates get over grown (like I often do)... then you gotta walk a fine line along the edge pieces or the mycelium thats reached the petri wall will hold on and Tarzan swing outta that plate and zing right past the jar to the floor... very frustrating... lol



A video would be nice!

So basicale tic tac toe, run the blade to free them from the petri and then dump right into the jar?

That dumping is what worries me the most, I would have to put my entire hand and plate above the opening of the jar... That would make me really unconfortable. Do you use a SAB or hood?





I do it ecactly like this. Cut the wedge, keep it above jar and scoop out the pieces real quick


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OfflineSankhara
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Re: Cultivation General Discussion [Re: stareatclouds] * 1
    #27216253 - 02/20/21 08:16 AM (2 years, 11 months ago)

Quote:

stareatclouds said:
Is there any reason you don't want to use PP's for this? It's so much easier.




Yeah... Argentina doesnt have those glad mini rounds

All I have are shitty pp deli cups which suck and normal petris.

I'll give a try to that technique you suggested! Its sounds like i could get it to work


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: Phony Phone]
    #27216443 - 02/20/21 10:22 AM (2 years, 11 months ago)

Quote:

Phony Phone said:
Guys, help me out here please.
There was a tek where you employ small amounts of spawn to germinate spores suspended in liquid syringe. I can't seem to find it.
If anyone knows what I am talking about, please link it here
thanks





You mean like a quarter pint jar of grain? People will do that and then take a colonized grain to agar to clean it up for species or spores that are stubborn.


Brf plates are good for syringes too since the bacteria can’t spread.


--------------------
LAGM2020


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OfflinePhony Phone

Registered: 09/19/20
Posts: 612
Last seen: 6 days, 18 hours
Re: Cultivation General Discussion [Re: A.k.a]
    #27216529 - 02/20/21 11:23 AM (2 years, 11 months ago)

Quote:

A.k.a said:



You mean like a quarter pint jar of grain? People will do that and then take a colonized grain to agar to clean it up for species or spores that are stubborn.


Brf plates are good for syringes too since the bacteria can’t spread.




Yup that's what I am talking about. I am thinking to do it for pans since I have some LC that doesnt germinate on agar and no prints. Was thinking to do it with manure/grain mix but will rye by itself work for pans? Grains alone without manure would be far easier to pluck from and then clear on agar


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: Phony Phone]
    #27216550 - 02/20/21 11:34 AM (2 years, 11 months ago)

Yeah just grain is fine, that’s pretty common with pans to get them to germinate.

Lc is living mycelium in a liquid though, spore syringe is what we’re talking about.


LC



--------------------
LAGM2020


Edited by A.k.a (02/20/21 11:34 AM)


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Offlineclockworkshroom
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Re: Cultivation General Discussion [Re: A.k.a]
    #27216731 - 02/20/21 01:15 PM (2 years, 11 months ago)

Is this foam ball thang in the centre bad news? Its not good spawn so am expecting it to fuck up sooner or later



--------------------
p9hu7's clean spawn thread


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InvisibleLenz
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Registered: 08/17/20
Posts: 692
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Re: Cultivation General Discussion [Re: stareatclouds]
    #27216832 - 02/20/21 02:17 PM (2 years, 11 months ago)

Quote:

stareatclouds said:
The wedges can break, but once you get a good feel for it, it's easy to get it done. You can also stab through multiple tic-tac-toe wedges with the blade, too. Making your agar harder and thicker (pause) will help with this. Also, 1/4 of a regular-sized Petri is more than enough for a 100ml LI. Perhaps you know that, though.

Is there any reason you don't want to use PP's for this? It's so much easier.




Somehow never thought to stab more than one piece at a time lol


How fast do your jars generally colonize with only a quarter of a plate in the LI? I usually do one plate to 250mL of water to ten jars and they colonize in 10-14 days but if I could just do like half a plate per LI that'd be sick.


--------------------
:chems:


Edited by Lenz (02/20/21 02:17 PM)


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Offlinetedoro
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Registered: 02/06/15
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Re: Cultivation General Discussion [Re: Lenz]
    #27216868 - 02/20/21 02:37 PM (2 years, 11 months ago)

I've been having good luck buying taller petri dishes and pouring them very thick. I wait for the plate to colonize thoroughly and use food coloring to show its progression. A quarter slice is like a whole plate.


--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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Invisiblestareatclouds
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Re: Cultivation General Discussion [Re: Lenz]
    #27216982 - 02/20/21 03:31 PM (2 years, 11 months ago)

Quote:

Lenz said:
Somehow never thought to stab more than one piece at a time lol


How fast do your jars generally colonize with only a quarter of a plate in the LI? I usually do one plate to 250mL of water to ten jars and they colonize in 10-14 days but if I could just do like half a plate per LI that'd be sick.




I'm not sure on 1/4 wedge. In general, I'd say my LI batches hit 100% between 12-18 days, but I'd often spread a 100-120ml jar pretty thin. Like often to 15-18 quarts at once. And I wouldn't always use 1/4 (a 1/2 is just as easy to maneuver). I liked to maximize my spawn since I didn't love sterile work. And with enough stuff going on, I didn't care if hitting 100% took an extra couple of days.


Edited by stareatclouds (02/20/21 03:40 PM)


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