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Offlinehazyhorse
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Re: Cultivation General Discussion [Re: fahtster]
    #27208521 - 02/15/21 10:15 PM (2 years, 11 months ago)

any of you ever have an agar wedge just not work? lol i shook these jars a few days ago & one of them had no colonization of any grains. i knew i hadn't accidentally put 2 wedges in the same jar, so i just assumed maybe the wedge had just been dead center or something. when i shook it i made sure it was on the glass so i could watch it develop. seems to be colonizing fine now? i'm assuming it just got stuck on the lid & i didn't notice or something but just thought it was funny



for reference here are the what the other jars i shook look like



--------------------
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i love glass petris & you can too!!
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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Cultivation General Discussion [Re: hazyhorse]
    #27208639 - 02/16/21 12:06 AM (2 years, 11 months ago)

Yall casing everything now adays or jst PE varieties?


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Offlinehazyhorse
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Re: Cultivation General Discussion [Re: Feasoghorm]
    #27208659 - 02/16/21 12:35 AM (2 years, 11 months ago)

i case/pseudocase at spawn with leftover coir & verm just because my climate is pretty dry. if you mean more traditional casing, i donโ€™t prefer it. havenโ€™t grown PE yet but it does seem to be almost necessary (or at the very least, incredibly beneficial) for proper pin sets


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ


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InvisibleTedsDead
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Re: Cultivation General Discussion [Re: hazyhorse]
    #27208725 - 02/16/21 02:24 AM (2 years, 11 months ago)

On ky standard tubs I use 4 qts CV for a toplayer. Ends up being an inch+. Makes marvest easier too.

Including toplayer spawn ratio is about 1:3-4


--------------------
weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396


Edited by TedsDead (02/16/21 02:26 AM)


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InvisibleMadHatter333
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Re: Cultivation General Discussion [Re: clockworkshroom]
    #27208741 - 02/16/21 02:54 AM (2 years, 11 months ago)

Quote:

clockworkshroom said:
thanks Roarkell, Madhatter, do you dry and use the caps after printing?



Yes, the caps are still good after printing.


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TEKs I Like


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OfflineFietchen
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Re: Cultivation General Discussion [Re: MadHatter333]
    #27208753 - 02/16/21 03:14 AM (2 years, 11 months ago)

I made a mistake and open the lid 4 day's ago

This is how my Tube has looked after i open the lid

I close the lid again and watching today because i thought it's ready but nothing has change from the Mycelium Growth its litte hard to see because the foil is on. What i need to do know?



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OfflineSchemenhaft
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Re: Cultivation General Discussion [Re: Fietchen]
    #27208764 - 02/16/21 03:28 AM (2 years, 11 months ago)

Quote:

Fietchen said:
I made a mistake and open the lid 4 day's ago

This is how my Tube has looked after i open the lid

I close the lid again and watching today because i thought it's ready but nothing has change from the Mycelium Growth its litte hard to see because the foil is on. What i need to do know?






Maybe thats another way of growing and I havent heard about it yet but why should it bea big deal to open the lid ?
Could you give more details what u are doing?

I mean, even if you open the lid and don't close it again, you just need to deal with the drying cake, especially the top-layer.
But if you keep an eye on it and you spray water on it(not directly) to keep it moist, it everything should be fine.
Means the lid is just for more easy handling and more condition-control but you could also grow open-air, needs just more work.


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InvisibleMadHatter333
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Re: Cultivation General Discussion [Re: Schemenhaft]
    #27208790 - 02/16/21 04:08 AM (2 years, 11 months ago)

That doesnโ€™t look like foil, looks like plastic wrap. Usually when you get your substrate to proper field capacity you donโ€™t have to take the lid off until you see mushrooms pinning. Increase FAE when you see a good pin set. You want small beads of moisture on the surface and that happens by itself if you have good field capacity substrate. You also want to let it evaporate because thatโ€™s what causes pinning. How long has it been in your tub?


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OfflineFietchen
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Re: Cultivation General Discussion [Re: Schemenhaft]
    #27208806 - 02/16/21 04:29 AM (2 years, 11 months ago)

Quote:

Schemenhaft said:
Quote:

Fietchen said:
I made a mistake and open the lid 4 day's ago

This is how my Tube has looked after i open the lid

I close the lid again and watching today because i thought it's ready but nothing has change from the Mycelium Growth its litte hard to see because the foil is on. What i need to do know?






Maybe thats another way of growing and I havent heard about it yet but why should it bea big deal to open the lid ?
Could you give more details what u are doing?

I mean, even if you open the lid and don't close it again, you just need to deal with the drying cake, especially the top-layer.
But if you keep an eye on it and you spray water on it(not directly) to keep it moist, it everything should be fine.
Means the lid is just for more easy handling and more condition-control but you could also grow open-air, needs just more work.





Then I make another mistake it looks litte dry so i have spray direct a good amount of water direct on the surface, shit... But how i can spray indirectly? Have I lost know?

The tube is know 1 week old!

I grow KSSS strain. My plan was just grow some Shrooms hehe.. Yeah its a plastic wrap but the effect is the same then a close lid.. The lid is not the best don't close good that's why i make the plastic stuff. I hope i can finish my Grow. What i need to do know just wait? I scared to mist to much but the water is Destilled.


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OfflineSankhara
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Re: Cultivation General Discussion [Re: Fietchen]
    #27208827 - 02/16/21 05:11 AM (2 years, 11 months ago)

Its been 15 days since i spawned cubes to 1:4 they colonized in about 7 days, but still no signs of fruiting or even nots.

Is it normal?


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OfflineGastronomicus
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Re: Cultivation General Discussion [Re: Sankhara]
    #27208834 - 02/16/21 05:24 AM (2 years, 11 months ago)

Yes but pics would help to see if there's anything shady


--------------------
Make my Funk the P Funk, I wants to get Funked up

LAGM2024


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InvisibleMadHatter333
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Registered: 09/20/17
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Re: Cultivation General Discussion [Re: Fietchen]
    #27208857 - 02/16/21 05:56 AM (2 years, 11 months ago)

You mist indirectly by doing a fine mist over the top and letting it fall onto your surface. If it becomes to moist it can cause your surface to mat up and the only shrooms you will get is side shrooms. If itโ€™s only been a week Iโ€™d be patient and let it fully colonize before taking the wrap off. The good thing about using a lid though is itโ€™s not fully sealed so thereโ€™s still a little air exchange going on under the lid. Plastic wrap works too though, Iโ€™ve done some novelty grows with plastic wrap over the tops of my containers.


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OfflineSchemenhaft
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Re: Cultivation General Discussion [Re: Schemenhaft]
    #27208876 - 02/16/21 06:18 AM (2 years, 11 months ago)

Quote:

Schemenhaft said:
Could someone just take a look on those Agar-plates ?
Five out of 10 Transfer-plates/Plates opened in this SAB-session show germination like this.
Means I have germination on the old plates and also on the new ones - Before everything was looking clean.



Could it be just also Cubensis-Mycelium - Idk, because a bit of water took tiny pieces of myc to other spots?
It looks same like my Plates with Multispore inoculation when I had water flowing a bit around spreading all the spores around - but most of these plates are clone-plates - But I made clones from the caps - could it be just spores which were in the tissue of the cap I used to clone?

I hope its not mold - If its mold I think I have a problem in my Apartment then, that would be a crazy increase of fails in my SAB-Work since I have restarted shrooming.
Had a MonoTub getting Moldy after third flush around one week ago (with trich) - But I have tossed everything instantly and the Tub is still outside the house - And the trich was still white, so it shouldnt have sporulated.

But if the scenario is correct, all these plates are contaminated by mold-spores because of increased amount of spores in my house (because of a failed grow) - What could I do to fix this as fast as possible?
Wanted to do around 35 jars in the next days when my masters are ready.
But under this circumstance I would just ruin my spawn and waste all my masters+rye+maybe cvg (and time)





Any information how to fix this?
I am really scared to do my g2g tomorrow, don't want to ruin 30 jars of rye and many masters.
The stuff on the pics is 100% mold, today in the morning it was clear!


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OfflineGastronomicus
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Re: Cultivation General Discussion [Re: Schemenhaft]
    #27208882 - 02/16/21 06:31 AM (2 years, 11 months ago)

Everyone's house is full of mold spores. As long as you're working in a SAB and you're not messing up your sterile technique it should be ok. You're sure your master is clean?


--------------------
Make my Funk the P Funk, I wants to get Funked up

LAGM2024


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OfflineSchemenhaft
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Re: Cultivation General Discussion [Re: Gastronomicus]
    #27208893 - 02/16/21 06:45 AM (2 years, 11 months ago)

@Gastronomicus

I made around 80 Agar-Plates in the last 2 months, 90 of those which should be clean were clean, so my sterile technique should be not that bad.
Also 12 this year, 2 ended up moldy, Rest was fine or is fine till now.

Maybe I just failed at setting up my SAB when I made these posted agar-plates and thatโ€™s why I got mold on all of these.
Or the moldy tub spored my house - But the trich-contamination hasnโ€™t turned green and it was a tiny spot.
But thatโ€™s why I am scared that I got a increased amount of Trich-spores in my house.
I tossed the tub instantly outside but I opened the lid before inside before I have seen the tiny spot of mold.

My masters should be fine, made them before the tub showed trich.
They grow as usual, no sign of contamination, Iโ€™ll add some pics in 5 mins


Edited by Schemenhaft (02/16/21 06:47 AM)


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Offlineclockworkshroom
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Re: Cultivation General Discussion [Re: Schemenhaft]
    #27208902 - 02/16/21 06:53 AM (2 years, 11 months ago)

I'm not very experienced and the photos aren't great but I wanted to directly answer the question you are asking. I don't see clear mold on those plates, it could just be myc. Is it pure white? it's difficult to tell with the lighting in the photo but like you said it could just be spores that didn't land with the transfer that are germinating separately (looks like an ms syringe plate). Just transfer away from the good stuff in the centre.

Also, why is your agar so bubbly? do you shake it loads before pouring? you shouldn't need to.


--------------------
p9hu7's clean spawn thread


Edited by clockworkshroom (02/16/21 06:55 AM)


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Offlineclockworkshroom
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Re: Cultivation General Discussion [Re: Fietchen]
    #27208909 - 02/16/21 07:01 AM (2 years, 11 months ago)

Quote:

Fietchen said:
Quote:

Schemenhaft said:
Quote:

Fietchen said:
I made a mistake and open the lid 4 day's ago

This is how my Tube has looked after i open the lid

I close the lid again and watching today because i thought it's ready but nothing has change from the Mycelium Growth its litte hard to see because the foil is on. What i need to do know?






Maybe thats another way of growing and I havent heard about it yet but why should it bea big deal to open the lid ?
Could you give more details what u are doing?

I mean, even if you open the lid and don't close it again, you just need to deal with the drying cake, especially the top-layer.
But if you keep an eye on it and you spray water on it(not directly) to keep it moist, it everything should be fine.
Means the lid is just for more easy handling and more condition-control but you could also grow open-air, needs just more work.





Then I make another mistake it looks litte dry so i have spray direct a good amount of water direct on the surface, shit... But how i can spray indirectly? Have I lost know?

The tube is know 1 week old!

I grow KSSS strain. My plan was just grow some Shrooms hehe.. Yeah its a plastic wrap but the effect is the same then a close lid.. The lid is not the best don't close good that's why i make the plastic stuff. I hope i can finish my Grow. What i need to do know just wait? I scared to mist to much but the water is Destilled.




The lid doesn't need to be air tight at any point, I would revert back to the standard lid is it see thru? Can you buy a better tub? a plastic shoebox is like ยฃ2 in the UK.

Spraying directly unless you are blasting it isn't going to completely ruin your grow so no you haven't lost but you should just mist above. You may get less ideal fruiting on the top than you would have but you won't have terminated the grow purely by spraying it. 

I'd just make some tiny holes in the cling wrap near the edges and wait.


--------------------
p9hu7's clean spawn thread


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OfflineSchemenhaft
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Re: Cultivation General Discussion [Re: clockworkshroom]
    #27208914 - 02/16/21 07:05 AM (2 years, 11 months ago)

Quote:

clockworkshroom said:
I'm not very experienced and the photos aren't great but I wanted to directly answer the question you are asking. I don't see clear mold on those plates, it could just be myc. Is it pure white? it's difficult to tell with the lighting in the photo but like you said it could just be spores that didn't land with the transfer that are germinating separately (looks like an ms syringe plate). Just transfer away from the good stuff in the centre.

Also, why is your agar so bubbly? do you shake it loads before pouring? you shouldn't need to.



Quote:

clockworkshroom said:
I'm not very experienced and the photos aren't great but I wanted to directly answer the question you are asking. I don't see clear mold on those plates, it could just be myc. Is it pure white? it's difficult to tell with the lighting in the photo but like you said it could just be spores that didn't land with the transfer that are germinating separately (looks like an ms syringe plate). Just transfer away from the good stuff in the centre.

Also, why is your agar so bubbly? do you shake it loads before pouring? you shouldn't need to.





Yesterday I was also hoping it is myc, but today I am 100% sure its mold - The different "circles" are clearly visible now - greyish from inside and getting more and more white to the outer circle an its growing way to fast for myc.
So like 50% of this SAB-run are moldy - Thats almost the same amount of failed plates I got all in all since I have restarted this hobby in december.
These plates are from around mid of last week.


Poured new plates saturday night and also did some transfers - If these plates also show mold-germination everywhere I'll gonna have a big problem for sure.
If these are all good it was maybe just a big mistake by myself while setting up my SAB at this day - or something similar.

Idk why this charge of agar is so blobby usually its clear, i think there is a relation to the red foodcolor, the other plates at the same time with different color are all fine.
Usually I don't shake it because I pour hot - But not 100% sure, maybe I did, idk.


@Gastronomicus

Here the pics of my masters I'll wanna use in the next days.


--------------------


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Offlineclockworkshroom
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Re: Cultivation General Discussion [Re: Schemenhaft]
    #27209041 - 02/16/21 09:15 AM (2 years, 11 months ago)

First ever print just completed, folded and stored. I love this stuff so much :smile: wish I'd found it years ago.


--------------------
p9hu7's clean spawn thread


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OfflineSockadin
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Re: Cultivation General Discussion [Re: hazyhorse]
    #27209078 - 02/16/21 09:35 AM (2 years, 11 months ago)

Quote:

hazyhorse said:
any of you ever have an agar wedge just not work? lol i shook these jars a few days ago & one of them had no colonization of any grains. i knew i hadn't accidentally put 2 wedges in the same jar, so i just assumed maybe the wedge had just been dead center or something. when i shook it i made sure it was on the glass so i could watch it develop. seems to be colonizing fine now? i'm assuming it just got stuck on the lid & i didn't notice or something but just thought it was funny



for reference here are the what the other jars i shook look like







  Both these jars look contaminated. The bacteria is probably what is preventing them from growing out.


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