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Vinci
I'm You



Registered: 08/26/19
Posts: 629
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Quote:
clockworkshroom said: I'll do some reading, I thought LC only lasted a couple of months. I was talking a year or so.
To store cultures, generally you want: Media that contains enough nutrients that your shit won't starve A tiny bit of fae so you don't suffocate or dry out your culture Low temps to slow the digestion/growth
Catch them 3 thangs and you should be gucci. An LC jar with a sfd hole in the lid, thrown in the fridge at 50% colonization works well for long storage
-------------------- Vinci loves you
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CocaineBuffet
Stranger



Registered: 08/29/19
Posts: 3,459
Last seen: 13 minutes
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Re: Cultivation General Discussion [Re: Vinci]
#27181544 - 02/01/21 06:38 PM (2 years, 11 months ago) |
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What is the difference between leucistic golden teacher and true albino teacher?
Is the marking of a true albino mean it doesn't drop spores?
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junk_f00d


Registered: 12/04/15
Posts: 933
Last seen: 1 year, 2 months
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same as the difference between a true albino and a leucistic (pure white, not still kinda creamy), albinos drop clear spores from what I've heard.
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Celestialexplorer1


Registered: 03/25/20
Posts: 1,803
Loc: Floating in a tin can
Last seen: 2 months, 13 days
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Re: Cultivation General Discussion [Re: junk_f00d]
#27181740 - 02/01/21 08:42 PM (2 years, 11 months ago) |
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Albinos have albino spores. Leustic are just light in color and are mainly bread to mimic the look of an albino but drop natural spores
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 To spend just one moment in eternity
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clockworkshroom
Stranger


Registered: 02/20/20
Posts: 655
Last seen: 2 years, 7 months
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are albino spores visible on swabs? I got some Avery's swabs and they do have some colour. Still on agar at the moment
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Just_A_Noob
Breathing



Registered: 12/30/16
Posts: 6,809
Loc: PNW
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thats normal. I dont think its the spores though
-------------------- Wearing a mask is bad for my physical, emotional, and spiritual health. Complying = Consent Wide Mouth 1/2 Pint No-Pour TEK TC Teks & Links
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clockworkshroom
Stranger


Registered: 02/20/20
Posts: 655
Last seen: 2 years, 7 months
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Re: Cultivation General Discussion [Re: Just_A_Noob]
#27182637 - 02/02/21 10:43 AM (2 years, 11 months ago) |
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you don't think it is the spores on the swabs?
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fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,270
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Bruising juice.. spores are clear
Faht
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clockworkshroom
Stranger


Registered: 02/20/20
Posts: 655
Last seen: 2 years, 7 months
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Re: Cultivation General Discussion [Re: fahtster]
#27182699 - 02/02/21 11:14 AM (2 years, 11 months ago) |
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Got it. Thanks guys
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Culliganman360
Stranger



Registered: 06/05/19
Posts: 854
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So I lost 40 tubs. Out of 100 💔 To green mold and cobweb looking mold.
It’s Always on top of the sub. never below surface.
First I thought it was the way I’m preping grain. (No prep wbs) If that was the case why dident all my tubs contaminate?
I thought it was my inoculate which was 2 different lc jars. Same strain. If that was the case why dident all my bags and tubs turn green.
My bags looked good for the most part. I thought I knew what to look for They smelled like mush when spawning.
I’m busting my head trying to understand where I fucked up And how I can prevent this.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Do you have a spawn checklist for visual clues? I go over a mental checklist while observing my spawn. Bad spawn can smell good at spawning, this is the least reliable in my experience.
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Culliganman360
Stranger



Registered: 06/05/19
Posts: 854
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27183548 - 02/02/21 08:03 PM (2 years, 11 months ago) |
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I just know to look for •uncolonized grain •Lighter or thicker myc growth •any other color but white •slow growth &• recovery after a shake . That’s mostly it I think Clue me in if I’m missing something bro
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BrownBear
Warrior-Traveler



Registered: 06/05/20
Posts: 1,539
Last seen: 8 months, 7 days
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What are you using for substrate? Do you have any kind of air moving in the room where the tubs are? When exactly are you noticing green mold? Is it during colonization, before pinning, after pinning?
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Your spawn can smell decent and still be contaminated. Some of the things that I look for in my spawn is:
1. Colour and consistency. Is it an absurd colour of white, does it have bright white splotches or very pronounced, spiderweb like rhizomorphic tendrils. Does the mycelium have a thick ice cream like consistency;
2. Does it run on the slow side or have slow recovery. If I don't see fuzzy grains in 18 to 24 hours I'm very suspicious;
3. Is it hard as fuck to shake the grains apart during the shake? Like, do you have to smash that jar so hard that it almost hurts your hand? If it's that tenacious then it's not likely good, at least for cubes;
4. Do my jars look overly wet with lot's of interior condensation, some condensation is ok, its just an indication that it's warmer inside the jar than outside of the jar and is to be expected due to the presence of a living organism. Heavy condensation however can be an indication of bacterial activity;
5. The above is often accompanied by uncolonized, wet looking individual grains dispersed throughout your jar or bag, often times pushed up against the glass or plastic. These grains are often accompanied with the super white ice cream like mycelium as well. Sometimes you will have groups of grains that have been avoided by the mycelium altogether, they sit untouched no matter how long you wait.
If any of those things are off I don't even bother with it, I just toss it and save myself the headache. I always have lots of spawn to fuck with anyways so it's not a big deal.
Your spawn should be easy to shake, should be uniform in colour and consistency, fully colonized and lastly it should smell good at spawning but smell isn't always a good indicator.
So this is what I personally use as a mental checklist when observing my spawn. Hopefully this will help. If you're risking that many tubs I'd be very careful with spawn selection.
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GypsyBastard
Misanthrope


Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
Last seen: 2 years, 10 months
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Maybe a cross post but also a bit of an opinion poll. When I first got into mush cult it seemed like 90 min was the standard PC time. Later on I read many more people using 120 mins so I followed their lead. So I'm curious, how long do you all PC grain jars?
I ask because after forgetting I was heating my PC and venting much longer than normal, my PC ran dry (couldn't maintain pressure) at about the 109 min mark. I have pt jars, half pt jars, and a syringe and beaker in there so I think 109 will be ok but it got me thinking about what the norm is now days.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: GypsyBastard]
#27183592 - 02/02/21 08:22 PM (2 years, 11 months ago) |
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I run jars for 2 hours, bags for 3.5 to 4.
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Culliganman360
Stranger



Registered: 06/05/19
Posts: 854
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27183609 - 02/02/21 08:26 PM (2 years, 11 months ago) |
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brownbear- I’m growing in A old basement under a coop only air flowing through is from the windows that aren’t properly sealed and me entering/exiting grow room .
First time mold popped up was about a week into colonizing . Lost 5 tubs . Everyday after that it’s been like 5 tubs here 10 tubs there mostly all before pinning, I had 3 that started to show green after a nice ass pinset.
@p9-thank you for dropping that knowledge bro. Definitely gonna save it. I gave up jars after using bags, and my no prep bag method leaves me with clumpy mushy ass grains at the bottom of my bags after pc cycle I just mush it all in with the top layer that looks good . I was seeing what I thought was good growth but I did have lots of condensation inside bags . And if I let bags sit colonized for too long they get a clear slimy look on the bottom of the bag in the guisset folds. Think this is likely my main problem ?
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tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,206
Last seen: 22 hours, 25 minutes
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27183624 - 02/02/21 08:32 PM (2 years, 11 months ago) |
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I'll chime in that no-prep grains leave uneven hydration (the top get bare as the water level drops) some grains become hard to sterilize if not properly hydrated first. I was having your exact problem... contams on the surface because I wasn't able to sterilize my wheat berries because of no prep. Just a thought.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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GypsyBastard
Misanthrope


Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
Last seen: 2 years, 10 months
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27183629 - 02/02/21 08:33 PM (2 years, 11 months ago) |
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Quote:
p9hu7 said: I run jars for 2 hours, bags for 3.5 to 4.
Hypothetical: your 2 hour cycle stopped 11 mins shy. Do you run them again? This was a bit of a test run for a no-prep ratio so I'll likely use them assuming they come out not too dry\wet. It's only 3 pts and 4 half pts so not a huge loss if things go pear shaped. But I'm curious if I'm being lax in my risk assessment.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Slime is likely bacteria, might just be starches though, I'd say it's your grain prep. I don't fuck around with no prep because of that clumpy dark bottom layer of grains. I soak my millet overnight and then load, grains come out fine without getting cooked to the bottom.
No, 11 mins is nothing.
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