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mushhead
Potato Devourer



Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
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Quote:
clockworkshroom said: I will smell for sure but a) I have a terrible sense of smell b) I had covid a few weeks ago so it's even worse than usual and c) I haven't smelt molds before. So whilst I planned to I don't particularly trust my nose for this job 
So just do as I had suggested, spawn it to a shoebox and let it go, either you end up throwing it out or you get mushrooms. The spawn honestly doesn't look bad to me. I got way lucky with a recent culture of Burmas that's super fast, so do some observation on it while its in the shoebox. Take notes, if it is contaminated you'll have documented learning material.
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Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house
Last seen: 6 hours, 14 minutes
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You couldn't imagine some of the ugliness I've had and my place is still good. I've had shit so ugly I just threw the whole fuckin tub away. I didn't even wanna attempt to wash it. I've got shoeboxes that are stained green from trich that still work perfectly. Don't know how but it does
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 7 hours, 33 minutes
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Yeah determining if spawn is good or not has always been my toughest area.
As long as you’re doing the SAB right contams don’t matter, I had probably one of the worst possible scenarios for molds and did fine. There are threads of people posting the nasty spots the work in, mold all over the walls and stuff.
Working in spots like that will expose holes in your SAB game real quick too.
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LAGM2020     
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: A.k.a]
#27174867 - 01/28/21 04:05 PM (3 years, 1 day ago) |
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Do you guys always blame your inoculant or technique for bacterial spawn? I mean, if I was a noob that didn't know how to make clean inoculant or didn't trust my technique then sure, I'd blame myself.
Making clean/axenic inoculant is retarded easy, however I don't think it's easy to make perfect spawn simply because endospores are able to survive a PC cycle like a bitch from hell... bad prep or bad source for grain can do that for you easy, in my humble fucking experience.
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BrownBear
Warrior-Traveler



Registered: 06/05/20
Posts: 1,539
Last seen: 8 months, 7 days
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Re: Cultivation General Discussion [Re: Josex]
#27174878 - 01/28/21 04:12 PM (3 years, 1 day ago) |
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I almost always have atleast some bacteria when I use tsc oats. It doesn't matter whether I rinse them first, boil the shit out of them and pc them for 3 hours. On the other hand, if I use grocery store grade grains such as rice and popcorn, I see no signs of bacteria in my spawn. Not that the slightly bacterial oat spawn causes any problems during fruiting but it is a nuisance.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: BrownBear]
#27174893 - 01/28/21 04:19 PM (3 years, 1 day ago) |
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I had the same issue with oats from tsc. I would also get some bacteria from undercooked wheat, so far I've had no issues with millet.
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Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house
Last seen: 6 hours, 14 minutes
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Re: Cultivation General Discussion [Re: BrownBear]
#27174897 - 01/28/21 04:20 PM (3 years, 1 day ago) |
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All I used are tsc oats. Never really had a problem from them that wasn't my fault. I can get wheat for the same price but I don't like the way it colonizes, just like white millet. They never look like they are done colonizing
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: Crackatoa]
#27174903 - 01/28/21 04:24 PM (3 years, 1 day ago) |
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That's normal with millet. If it wasn't for the price that's what I'd rock
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 7 hours, 33 minutes
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Re: Cultivation General Discussion [Re: Crackatoa]
#27174909 - 01/28/21 04:26 PM (3 years, 1 day ago) |
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I always assume it’s my fault unless it’s an obvious pattern.
I had this one type of wbs that would go bacterial after about 10 days every jar. No idea what was in it that caused that.
I’m sure sometimes it’s not caused by me but when I’ve got all the prep type stuff dialed in it’s something I messed up more often than not.
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LAGM2020     
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BrownBear
Warrior-Traveler



Registered: 06/05/20
Posts: 1,539
Last seen: 8 months, 7 days
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Re: Cultivation General Discussion [Re: A.k.a]
#27174920 - 01/28/21 04:41 PM (3 years, 1 day ago) |
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I'm not really sure how it could be my fault with the tsc oats. Its not like I have a bunch of busted grain and they aren't over hydrated. Everything else is exactly the same. The only variable is the type of grain. That's it. I have used the same culture and method of inoculation. I see a difference with a slower colonization rate with visible bacteria when it comes to feed grade oats vs grocery store grade grains.
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: Cultivation General Discussion [Re: BrownBear] 1
#27174934 - 01/28/21 04:52 PM (3 years, 1 day ago) |
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Would be fun to see peoples g2 spawn under a scope and see if anyone has zero bacteria.
Now who wants to go first
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: filthyknees]
#27174938 - 01/28/21 04:58 PM (3 years, 1 day ago) |
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I'm ordering a scope soon, I'll be running almost everything under it. My primary focus will be on bacteria present in spent exotic substrate, but sampling random mycelium to see how clean it really is sounds interesting as well.
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BrownBear
Warrior-Traveler



Registered: 06/05/20
Posts: 1,539
Last seen: 8 months, 7 days
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Re: Cultivation General Discussion [Re: filthyknees] 1
#27174940 - 01/28/21 04:58 PM (3 years, 1 day ago) |
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I highly doubt anyone has zero bacteria in their spawn regardless of what type of grain they use and how they prep it. Just to be clear, I wasn't stating that the grocery store grains don't have bacteria. It's just that it is not noticeable
Edited by BrownBear (01/28/21 05:00 PM)
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: Cultivation General Discussion [Re: BrownBear]
#27174943 - 01/28/21 05:01 PM (3 years, 1 day ago) |
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I sure as fuck have seen some bacteria before just having some fun tehe.
But interesting none the less!
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: filthyknees]
#27174952 - 01/28/21 05:07 PM (3 years, 1 day ago) |
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It would be interesting to id whatever bacteria is tagging along without causing much harm, if there actuallyis any. We know what kinds to avoid, are there any bacteria hitching a ride without issue though? Probably pointless to know, still interesting though.
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 6 hours
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27175185 - 01/28/21 07:43 PM (3 years, 1 day ago) |
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are we talking bacteria from the grain not being fully sterilized or from inoculation? Or from the culture used?
I see very little bacteria on agar if ever(unless cleaning up spores or clones) and although noccing up bags is much riskier I don't think I'm adding much bacteria if any during inoculation.
So then insufficient sterilization? And how do we get spawn without bacteria? Grocery store grain or rice? And is it worth it in the end to have bacteria free spawn?
I use wheat primarily but I was using oats from a feed store and saw similar results to wheat. Oats just looks less colonized and made less thick of colonies olverall compared to wheat. But a mix of the two was pretty nice as well.
I had serious bacteria issues from insufficient sterilization because my pressure gauges on my sterilizers were failing and reading a higher pressure than they were actually at. Something like 1/3 to 2/3 of my grain was going bacterial all of a sudden. This is the second time that this has happened so I knew right away what the problem was but the first time around I thought it was me or my innoculant because it wouldn't show it's face until the myc started colonizing. So it took a lot of troubleshooting to narrow the problem down. Thought it was my filter for a bit and I ordered a new one that is still in it's box as I found the pressure gauges were the real issue.
I saw someone post here(I think here and can't remember who) some time ago asking about bacteria not showing up until after being inoculated and that is certainly the case for me. It shows no signs of problem(or smell until the myc starts colonizing it.
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Edited by tombosley8 (01/28/21 09:06 PM)
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junk_f00d


Registered: 12/04/15
Posts: 933
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: tombosley8]
#27175198 - 01/28/21 08:02 PM (3 years, 1 day ago) |
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Quote:
tombosley8 said: I had serious bacteria issues from insufficient sterilization because my pressure gauges on my sterilizers were failing and reading a higher pressure than they were actually at.
Off topic, but if you're running something like a Presto, it's a good idea to just go by the sounds it makes at a given PSI. My guages are broken, but I just listen to the weight rock and hiss and dial down to where I know 15-18PSI would be.
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BrownBear
Warrior-Traveler



Registered: 06/05/20
Posts: 1,539
Last seen: 8 months, 7 days
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Re: Cultivation General Discussion [Re: tombosley8] 2
#27175205 - 01/28/21 08:07 PM (3 years, 1 day ago) |
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I think it is common knowledge that us home cultivators cannot completely sterilize our grain with a pc. We are merely creating a window of opportunity for our culture to get a foothold on the substrate before anything else has a chance to grow on it. We can reduce the bacteria enough to have great success but cannot eliminate it completely.
https://en.m.wikipedia.org/wiki/Endospore
https://en.m.wikipedia.org/wiki/Thermophile
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bw86
Doesn't play well with others


Registered: 11/12/06
Posts: 5,937
Loc: 7b
Last seen: 2 days, 6 hours
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Re: Cultivation General Discussion [Re: filthyknees]
#27175257 - 01/28/21 08:52 PM (3 years, 1 day ago) |
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Im so fuckin fed up looking for the filter i want(30X30). I can find it @ 99.997 or i can find it @ the 11 inch depth but im not willing to do either of those.
Im gona go with this one here . Can someone just tell me if its proper. https://www.spycor.com/99-99-HEPA-Filter-Metal-Frame-24-x-36-x-5-7-8-p/maggb2436a6ax.htm
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junk_f00d


Registered: 12/04/15
Posts: 933
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: bw86]
#27175265 - 01/28/21 09:01 PM (3 years, 1 day ago) |
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Quote:
bw86 said: Im so fuckin fed up looking for the filter i want(30X30). I can find it @ 99.997 or i can find it @ the 11 inch depth but im not willing to do either of those.
Im gona go with this one here . Can someone just tell me if its proper. https://www.spycor.com/99-99-HEPA-Filter-Metal-Frame-24-x-36-x-5-7-8-p/maggb2436a6ax.htm
What's wrong with 99.997? Most people complain about 99.97, and purchase 99.99 instead, which is of course less than 99.997.
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