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Offlinetedoro
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Re: Cultivation General Discussion [Re: A.k.a]
    #27161857 - 01/21/21 04:08 PM (3 years, 8 days ago)

Quote:

A.k.a said:
Those bags are gh or the clean ones were?? Golden halo is known for having goofy myc.


Does anybody have suggestions on where and what kind of syringes to buy?? I want to learn to make them finally.

I know there’s all different size barrels and needle gauges. I think 10ml is the standard size barrel right? What about needle?




I've had numerous syringes over the years... and they keep working after dozens and dozens of 25 minute PC'ing. So I think any of them are made fine. I love huge ones. I used 60cc ones for years until I found 100cc. Look for the locking luer lock tip. They make smooth non-locking luer tips which are annoying.


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: tedoro]
    #27161881 - 01/21/21 04:18 PM (3 years, 8 days ago)

I actually found a set of Pyrex ones I might get for lc.


I’m all about the locks, I already got a big bag of those little black tips that hold the spot until you put the needle on.


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LAGM2020


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Offlinetedoro
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Re: Cultivation General Discussion [Re: A.k.a]
    #27161935 - 01/21/21 04:46 PM (3 years, 8 days ago)



I am trying to hunt down the cause of this green nasty that shows up during or before first flush. It seems to be around the last coir to colonize.

wheat, coir only, GT. photo taken at 8 days. no pinning yet.


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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OfflineCrackatoa
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Re: Cultivation General Discussion [Re: tedoro]
    #27161963 - 01/21/21 05:02 PM (3 years, 8 days ago)

Quote:

tedoro said:


I am trying to hunt down the cause of this green nasty that shows up during or before first flush. It seems to be around the last coir to colonize.

wheat, coir only, GT. photo taken at 8 days. no pinning yet.




Your spawn is dirty


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Crack's Tek's and Shit



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Offlinetedoro
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Re: Cultivation General Discussion [Re: Crackatoa]
    #27161971 - 01/21/21 05:08 PM (3 years, 8 days ago)

grrrrr.

I've been testing my LI back on agar and it looks lovely. My control bags that remain un-inoculated smell fine. I'm stumped.


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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Invisiblemushhead
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Re: Cultivation General Discussion [Re: tedoro]
    #27161996 - 01/21/21 05:26 PM (3 years, 8 days ago)

Quote:

tedoro said:
grrrrr.

I've been testing my LI back on agar and it looks lovely. My control bags that remain un-inoculated smell fine. I'm stumped.



Mold is tricky like that friend.
at least you haven't had to battle white pin mold or gray mold.
You don't even know its mold until you get it to the tub and suddenly its covered with a light gray mycelium that doesn't fruit and just smells like sugar.


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OfflineJarhead3521S
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Re: Cultivation General Discussion [Re: mushhead]
    #27161997 - 01/21/21 05:29 PM (3 years, 8 days ago)

Second the Tractor Supply for both syringes and needles.....waaaay cheaper.....think I just paid like $5-8 for a 20pk of sticks and it was like $12 for a box of 4 10ml syringes


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OfflineCrackatoa
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Re: Cultivation General Discussion [Re: tedoro]
    #27162069 - 01/21/21 06:01 PM (3 years, 8 days ago)

Quote:

tedoro said:
grrrrr.

I've been testing my LI back on agar and it looks lovely. My control bags that remain un-inoculated smell fine. I'm stumped.




It could get contaminated getting from one place to another. I might have great spawn but I might screw up and still contaminate during G2G transfers. I know you use LI but you know what I mean.


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Crack's Tek's and Shit



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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: Crackatoa]
    #27162088 - 01/21/21 06:16 PM (3 years, 8 days ago)

Yeah if it’s testing clean it’s either the grain isn’t totally sterile or stuffs getting in during inoculation.

Or it could just look like it’s testing clean sometimes you’ll get a real sneaky one.


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InvisiblePastywhyteMDiscord
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Re: Cultivation General Discussion [Re: A.k.a]
    #27162138 - 01/21/21 06:40 PM (3 years, 8 days ago)

Just because a grain bag looks clean doesn’t mean it is. I also find if I use more than 15cc of LC in a large bag it runs the risk of going bacterial unless it’s a very very thick LC.


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OfflineCelestialexplorer1
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Re: Cultivation General Discussion [Re: Pastywhyte]
    #27162144 - 01/21/21 06:42 PM (3 years, 8 days ago)

Yeah you kinda get a feel for how dry the grain vs how much LC you use. If my grains a little drier I know I can use more LC if it's perfectly hydrated o wouldn't use too much.


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To spend just one moment in eternity


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Offlinetedoro
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Re: Cultivation General Discussion [Re: Celestialexplorer1]
    #27162187 - 01/21/21 07:02 PM (3 years, 8 days ago)

I've been squeezing the control bag and smelling the patch. Just smells of wheat.

I'll test with less LI, thank you all for chipping in.


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


Edited by tedoro (01/21/21 07:03 PM)


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OfflineZsuzsi
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Re: Cultivation General Discussion [Re: tedoro]
    #27162217 - 01/21/21 07:17 PM (3 years, 8 days ago)

For those of you that have followed my troubles with my first monotub - posted about it here and here, an update.

I was starting to suspect too much water and condensation were at the root of the issue, so I used a fan pointed away from the tub. That still didn't do the trick, so I pointed the fan straight at the monotub's holes for 24 hours. After that time, not only did most of the water evaporate, the surface started to look much healthier. It's been 2 days since then and now, almost all of the metabolites/brown weird blotches on my myc are now almost entirely covered by new myc - like it somehow managed to regain ground due to the decreased constant pools of water. There's only one small spot that still persists thus far - the one that appeared first, but it's definitely not growing. I expect all the other to actually disappear below new myc soon, given the recent evolution.

So all in all, I'm in the end now feeling prudently optimistic for at least a first flush. Thanks a lot to all of you ! Due to the intense evaporation I was even hoping to see, perhaps, some hyphae; there are a few bright white spots that weren't there before but I don't think they're knots - though who knows. I'm trying to not raise my hope too much given the disappointment when I first saw the affected patches.

Of course maybe I'm a moron and the new white I'm seeing is now mold following bacteria, but at least on that part I don't think so - it didn't grow very fast, it looks exactly the same, and it smells exactly the same.


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Offline0ptiquest
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Re: Cultivation General Discussion [Re: Pastywhyte]
    #27162225 - 01/21/21 07:20 PM (3 years, 8 days ago)

Quote:

Pastywhyte said:
Just because a grain bag looks clean doesn’t mean it is. I also find if I use more than 15cc of LC in a large bag it runs the risk of going bacterial unless it’s a very very thick LC.




damn i have been pouring like 20ml of LC to my liter jars, good fuits but yes, probably a bit bacterial grains.


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InvisibleVinci
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Re: Cultivation General Discussion [Re: 0ptiquest]
    #27162234 - 01/21/21 07:26 PM (3 years, 8 days ago)

What's good with hot pour agar? Does anyone like the method?


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OfflineZsuzsi
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Re: Cultivation General Discussion [Re: Zsuzsi]
    #27162245 - 01/21/21 07:33 PM (3 years, 8 days ago)

I've only done hot pour agar, in a SAB. Followed c10's tek. I used an infrared thermometer as he recommends - the temp he gives is a good starting base though given the margins of cheap IR thermometers you'll want to try and get a feel of the best temp for you. Mine was around 56C, for example (he does it when his thermometer reaches 60C - or at any rate before it reaches 50C). There is some condensation on the top plate but a hot recipient above it fixes that relatively quick.

Beyond that, as long as you work quickly and cleanly, personally I haven't had any issue except at the beginning, and even then it was minimal (I pushed the hot syringe needle against the lower part's plastic of the dish, melting a small hole; that single dish out of 10 had any visible contam). Given that, I don't see the point of no-pour teks for me - bear in mind I have limited experience (my first tub is slightly bacterial but I'm 100% sure it wasn't the agar - most likely my spawn sterilization).


Edited by Zsuzsi (01/21/21 07:35 PM)


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OfflineGypsyBastard
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Re: Cultivation General Discussion [Re: Vinci]
    #27162262 - 01/21/21 07:38 PM (3 years, 8 days ago)

Can't remember if this was a topic here or the LAGM thread but we were talking To shake, or not to shake; here's my question:
I have a fuck load of half pints that are normally not in use. Other than having a fuck load of jars to work with is the a reason to not 4 half pints of WBS instead of 1 qt? Seems like you would get 100%colonized in approx. 1/4 of the time.
Yes, I know LC would be better if I'm looking for just faster spawn-ready jars. Just trying to think of ways to use what I have.


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OfflineZsuzsi
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Re: Cultivation General Discussion [Re: GypsyBastard]
    #27162292 - 01/21/21 07:55 PM (3 years, 8 days ago)

Quote:

GypsyBastard said:
I have a fuck load of half pints that are normally not in use. Other than having a fuck load of jars to work with is the a reason to not 4 half pints of WBS instead of 1 qt?




If you prepare them the same - 3/4 full and so on - then the only reason I see is the hassle of it. Which you seem OK with, so no issue that I can think of. I'm thinking of doing the same myself since I have like 20 small jars left over from an initial order when trying pf tek at the time (order was for 32 jars, only pf-tek-modified the cover of 12 of them)  - so I may do it too. Will probably use smaller wedges of agar for inoculation - like one dish for 6 or 7 jars instead of my usual 3 larger grain jars.

Though perhaps doing that would open one for slightly more potential contams, opening the dish that many times even under a sab.


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OfflineJarhead3521S
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Re: Cultivation General Discussion [Re: GypsyBastard]
    #27162295 - 01/21/21 07:56 PM (3 years, 8 days ago)

Quote:

GypsyBastard said:
Can't remember if this was a topic here or the LAGM thread but we were talking To shake, or not to shake; here's my question:
I have a fuck load of half pints that are normally not in use. Other than having a fuck load of jars to work with is the a reason to not 4 half pints of WBS instead of 1 qt? Seems like you would get 100%colonized in approx. 1/4 of the time.
Yes, I know LC would be better if I'm looking for just faster spawn-ready jars. Just trying to think of ways to use what I have.




Seems I wasn’t the only one on a jar scavenger hunt around the house tonight!


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InvisibleDoctor Mario
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Re: Cultivation General Discussion [Re: Jarhead3521]
    #27162365 - 01/21/21 08:46 PM (3 years, 7 days ago)

Yesterday, I drove 40 minutes to and from another town because it was the only place I could find quart jars and I wanted to get them before they were gone. Grabbed 3 cases. I made up 32 of these plastic lids with the adhesive SFD's. No more twisting polyfil.


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