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OfflineZsuzsi
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Registered: 03/05/19
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Re: Cultivation General Discussion [Re: Josex]
    #27155252 - 01/18/21 11:45 AM (3 years, 11 days ago)

Quote:

Josex said:
Yea that surface ain't looking pretty to me either, it looks bacterial. Well at least you have another tub looking good, this is all a learning experience for you.




Looking "good" in that it doesn't have as much condensation/pooling water, or metabolites, but the surface looks pretty much the same, sadly. And thanks again for your extremely valuable feedback, and for taking the time to answer.


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InvisibleJosex
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Re: Cultivation General Discussion [Re: Zsuzsi] * 1
    #27155287 - 01/18/21 12:01 PM (3 years, 11 days ago)

Those issues you're having have less to do with conditions and a lot more to do with the quality of your spawn. Looks like bacterial spawn is to blame for that. If you're unsure you can make a thread next time, explaining everything you do and posting pics of your plates and your jars so that people can help you out.


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Invisiblemushhead
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Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
Re: Cultivation General Discussion [Re: Josex]
    #27155311 - 01/18/21 12:08 PM (3 years, 11 days ago)

Quote:

Josex said:
Those issues you're having have less to do with conditions and a lot more to do with the quality of your spawn. Looks like bacterial spawn is to blame for that. If you're unsure you can make a thread next time, explaining everything you do and posting pics of your plates and your jars so that people can help you out.



This would be made a standard and if possible a banner should be placed above each new cultivation post haha.


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OfflineZsuzsi
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Re: Cultivation General Discussion [Re: mushhead]
    #27155321 - 01/18/21 12:17 PM (3 years, 11 days ago)

Agreed, since discovering this general cultivation thread I almost feel the mods should point towards it more often, perhaps with thread removals, and I almost feel guilty posting a whole new thread for the issue a week ago (the thread I linked earlier). Less random basic threads, with such content concentrated on great multithreads such as this one with helpful people, would help for the clarity of the forum.

And yes, Josex, I assume you're right - that's basically the universal conclusion I've read here: if it contams before first flush, your spawn is to blame. What worries me is I see nothing wrong I did this time, including with my sterile technique, so even more that I failed what sucks is that I don't know what to improve. I still have some pics of the plates and jars I took at the time, so I'll probably do a post-mortem comment asking for opinions if it fails, and will definitely request more feedback for future growths - I just felt like all was going so well this time it wasn't required (was even surprised at so few issues, compared both with my first PF tek and agar attempts - where I both times tried to change stuff a bit, so I felt sure that was part of the reason).


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Offlineclockworkshroom
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Re: Cultivation General Discussion [Re: Zsuzsi]
    #27155437 - 01/18/21 01:09 PM (3 years, 11 days ago)

Do y'all mist and fan watertubs?


--------------------
p9hu7's clean spawn thread


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OfflineSockadin
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Re: Cultivation General Discussion [Re: clockworkshroom]
    #27155450 - 01/18/21 01:13 PM (3 years, 11 days ago)

Whats a water tub?


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OfflineFailboat
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Re: Cultivation General Discussion [Re: clockworkshroom]
    #27155453 - 01/18/21 01:14 PM (3 years, 11 days ago)

I'm set and forget till I maybe flip the lid as the canopy flushes. If there's a concern/need to adjust moisture levels after spawning then contamination is aggressively present, IME.


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OfflineGypsyBastard
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Re: Cultivation General Discussion [Re: Failboat]
    #27155468 - 01/18/21 01:20 PM (3 years, 11 days ago)

Right after setting it I misted once a day until I figured out the right placement to not need to. I might give a quick spritz when I check on them but for the most part they haven't lost those microdroplets since the start. This is also my first time using a HC/WT instead of SGFC. Definitely seems much more simple.


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Offlineclockworkshroom
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Re: Cultivation General Discussion [Re: GypsyBastard]
    #27155481 - 01/18/21 01:27 PM (3 years, 11 days ago)

I still can't get droplets on mine haha but they feel damp enough. I have my lid flipped and skewed to allow a bit of Fae. Maybe I should close it for a bit?


--------------------
p9hu7's clean spawn thread


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Invisiblemushhead
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Registered: 08/22/14
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Re: Cultivation General Discussion [Re: Sockadin]
    #27155485 - 01/18/21 01:29 PM (3 years, 11 days ago)

Quote:

Sockadin said:
Whats a water tub?



a tub filled with water.


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InvisibleJosex
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Re: Cultivation General Discussion [Re: mushhead]
    #27155516 - 01/18/21 01:46 PM (3 years, 11 days ago)

:Awemazing:


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OfflineGypsyBastard
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Re: Cultivation General Discussion [Re: mushhead]
    #27155545 - 01/18/21 01:57 PM (3 years, 11 days ago)

ha, oh shit clockwork, didn't realize it was you that asked that. Kinda feel like an ass now!

I have my lid on right side up and not really skewed, just not latched. However the tub I'm using has 6 or 7 holes formed into the underside of the "handles" or hand grips so that the holes face straight down. I think this is helping a lot to allow FAE without losing too much RH.
You might try squaring up the lid but keeping it flipped, then next step would be squaring and unflipping lid. You can usually see them respond to changes within a day or so. To be completely honest though, I am "testing" so many variables with this current grow that I can't be sure what is causing what as far as growth, but RH does seem to be more spot on with this tek than I ever had with a SGFC.

Here is a pic showing the holes in the handle.


Quote:

Sockadin said:
Whats a water tub?






Also, for the record, the only reason there is a shoebox lid in there is because I was using the rack elsewhere when the first 4 cakes went in and when I switched them out those were already pinning and I didn't want to smoosh any by picking them up.


Edited by GypsyBastard (01/18/21 02:02 PM)


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: GypsyBastard]
    #27155567 - 01/18/21 02:07 PM (3 years, 11 days ago)

It’s a hc but with just water in there.


--------------------
LAGM2020


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Offlinestarbones
I'm an alien, I eat uranium.
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Registered: 03/04/20
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Re: Cultivation General Discussion [Re: A.k.a]
    #27155612 - 01/18/21 02:30 PM (3 years, 11 days ago)

No sir, I don't like that.

Feels like a pseudomonas breeding ground there.


--------------------
Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.




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InvisibleJosex
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Re: Cultivation General Discussion [Re: starbones]
    #27155617 - 01/18/21 02:32 PM (3 years, 11 days ago)

Quote:

starbones said:
No sir, I don't like that.

Feels like a pseudomonas breeding ground there.




I know you can't see me but I agree with you.

:singletear:


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Invisiblemushhead
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Re: Cultivation General Discussion [Re: Josex]
    #27155625 - 01/18/21 02:36 PM (3 years, 11 days ago)

I've used the water tub method before without bacteria getting in the way.
Just make sure the cakes aren't actually in the water itself.
However if you filled that tub with like Perlite or Coir to assist with evaporation and use four/five jars to hold the grate above the media the water is evaporating from you'll see better performance.
I can't remember who but someone around did a really amazing water tub grow and a Coir evap chamber.


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Offlinestarbones
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Re: Cultivation General Discussion [Re: mushhead]
    #27155674 - 01/18/21 03:07 PM (3 years, 11 days ago)

Right on.

Gonna try to take some fucking sketchy transfers from clone tissues that went bacterial on plates. Tissue itself began to jelly up but did develop fuzz on the top which did not get to the agar sadly.

Should be interesting trying to grab a few cells of living myc with the beveled edge of a syringe. A poke is one thing but this is gonna be absolute balls for a guy with shaky hands. Just need a few cells is all.


--------------------
Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.




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OfflineBondVillain
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Re: Cultivation General Discussion [Re: starbones]
    #27155712 - 01/18/21 03:23 PM (3 years, 11 days ago)

Question: can you skip the birthing phase? I had some BFR cake that were pinning inside the jars.

I birthed the cakes, dunked n’ rolled and have placed them into a SGFC. No visible growth yet (3 days).

I was wondering last night what would happen if I skipped the birthing phase (submerging the cakes for 24 hours) and had put the cakes directly into the fruiting chamber?


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Invisiblemushhead
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Re: Cultivation General Discussion [Re: BondVillain]
    #27155728 - 01/18/21 03:32 PM (3 years, 11 days ago)

Quote:

BondVillain said:
Question: can you skip the birthing phase? I had some BFR cake that were pinning inside the jars.

I birthed the cakes, dunked n’ rolled and have placed them into a SGFC. No visible growth yet (3 days).

I was wondering last night what would happen if I skipped the birthing phase (submerging the cakes for 24 hours) and had put the cakes directly into the fruiting chamber?



You should have birthed all the cakes that had fruit on them days ago mate like we said.
Also if you skip the rehydration stage your cakes will shrivel up and die from dehydration.
The cakes aren't that hydrated inside the jar by design, we don't want to smother the myc.
But after birthing you want to give the cakes what they need to fruit which is water.
EDIT: I mis-read, you did birth those cakes, awesome good job!!


Edited by mushhead (01/18/21 03:36 PM)


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Offlinejunk_f00d
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Re: Cultivation General Discussion [Re: mushhead]
    #27155771 - 01/18/21 03:50 PM (3 years, 11 days ago)

is there a good tek / write up that introduces breeding and crossing strains? There's a lot of terminology used in those threads that I haven't taken the time to learn proper, like F1 (which I think means offspring 1, just an example) and I don't have any intuition about when to "backcross" or anything.

I've read the rustywhyte thread but think it'd be great if something similar was done but all consolidated into one easy post, perhaps detailing the derivation of some new cross with each generation and the decisions that were made. i think I get the process mostly, but it'd just be nice to have it all collected in one spot and I'd be suprised if it didn't exist already.


Edited by junk_f00d (01/18/21 03:51 PM)


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