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Doctor Mario
πππππ



Registered: 08/07/20
Posts: 1,894
Loc: πΊπΈ
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Re: Cultivation General Discussion [Re: A.k.a]
#27154353 - 01/17/21 09:57 PM (3 years, 11 days ago) |
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I wish I was around at the beginning. I've missed all the history. I wanna know the history of all crosses and how we got to where we are today. I'm envious of the OG's. They got to see it all.
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Yeetusdeetus



Registered: 11/23/19
Posts: 1,242
Last seen: 4 hours, 27 minutes
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Re: Cultivation General Discussion [Re: Doctor Mario]
#27154392 - 01/17/21 10:31 PM (3 years, 11 days ago) |
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Exposure to radiation is used as a technique to encourage mutations in plant tissue culture iirc. I think they use gamma instead of uv though
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Doctor Mario
πππππ



Registered: 08/07/20
Posts: 1,894
Loc: πΊπΈ
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Re: Cultivation General Discussion [Re: Doctor Mario] 1
#27154444 - 01/17/21 11:17 PM (3 years, 11 days ago) |
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Quote:
Doctor Mario said: I wish I was around at the beginning. I've missed all the history. I wanna know the history of all crosses and how we got to where we are today. I'm envious of the OG's. They got to see it all.
I found this after making my last post. Its a very good read. Its funny cause the name PF is used every day I never even questioned what the abbreviation meant. I'm glad that I found this thread but theres still a lot of variety history that isn't included. Gonna have to keep digging through the search function to find out about the rest.
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Cultivation General Discussion [Re: Doctor Mario]
#27154868 - 01/18/21 07:47 AM (3 years, 11 days ago) |
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Cronicr has a strain thread in getting started that goes over a brief history of all the common varieties.
If you wanna go down some rabbit holes, read up on the history of penis envy
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GypsyBastard
Misanthrope


Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
Last seen: 2 years, 10 months
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Re: Cultivation General Discussion [Re: natedawgnow]
#27154895 - 01/18/21 08:03 AM (3 years, 11 days ago) |
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Glad I came to check the thread when I did. I'm not sure which thread but the PF and uv topic came up within the last week. That was the first I heard about it but it got me thinking, but then I forgot that I was thinking about it until now. I wanna dig a bit more and see what I can find but I can bombard myc with alpha and beta radiation all day long. The penetration is extremely low ( I think something like a 1mm) but in the early stages of development, 1mm would be enough to possibly have an effect of some kind. No clue what that effect would be but perhaps there is something to it....
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Doctor Mario
πππππ



Registered: 08/07/20
Posts: 1,894
Loc: πΊπΈ
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Re: Cultivation General Discussion [Re: natedawgnow]
#27154941 - 01/18/21 08:38 AM (3 years, 11 days ago) |
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Quote:
natedawgnow said: Cronicr has a strain thread in getting started that goes over a brief history of all the common varieties.
If you wanna go down some rabbit holes, read up on the history of penis envy
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junk_f00d


Registered: 12/04/15
Posts: 933
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Doctor Mario]
#27155027 - 01/18/21 09:34 AM (3 years, 11 days ago) |
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Got a thread in the marketplace where I'm looking for prints/swabs of Avery Albino and LPEU (offering PE/PEU/APE, PF-C syringes or GT/Redboy prints (all I have right now)). Not much interest there so I thought I'd try a post here to get more eyes, sorry if that's not allowed.
Edited by junk_f00d (01/18/21 09:53 AM)
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roarkell
The Roar Master!



Registered: 08/29/19
Posts: 2,223
Loc: Southern WI
Last seen: 6 months, 22 days
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Re: Cultivation General Discussion [Re: junk_f00d]
#27155065 - 01/18/21 09:57 AM (3 years, 11 days ago) |
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Quote:
junk_f00d said: Got a thread in the marketplace where I'm looking for prints/swabs of Avery Albino and LPEU (offering PE/PEU/APE, PF-C syringes or GT/Redboy prints (all I have right now)). Not much interest there so I thought I'd try a post here to get more eyes, sorry if that's not allowed.
I've got some fresh LPEU prints if you'd like to trade off a PEU...
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junk_f00d


Registered: 12/04/15
Posts: 933
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: roarkell]
#27155077 - 01/18/21 10:07 AM (3 years, 11 days ago) |
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Quote:
roarkell said:
Quote:
junk_f00d said: Got a thread in the marketplace where I'm looking for prints/swabs of Avery Albino and LPEU (offering PE/PEU/APE, PF-C syringes or GT/Redboy prints (all I have right now)). Not much interest there so I thought I'd try a post here to get more eyes, sorry if that's not allowed.
I've got some fresh LPEU prints if you'd like to trade off a PEU...

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Zsuzsi
Stranger
Registered: 03/05/19
Posts: 86
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Re: Cultivation General Discussion [Re: junk_f00d]
#27155121 - 01/18/21 10:35 AM (3 years, 11 days ago) |
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So I've got two small (~33qt) monotubs. One is slightly behind the other but looks fine, no problems there.
The other is now what looks like fully colonized. But I have a huge moisture issue - large amounts of condensation, which results in standing water. I've been wiping it from time to time but there are now 5 or 6 small spots full of metabolites, which suggests to me that that standing water brought bacteria along with it. And when I opened it today, jesus - I could've drunk from many places with a straw, so much water. And only on the surface - I drilled a small hole at the bottom when prepping the tub and covered it with tape, in case I ever need to remove water from the bottom, but the block of myc is still firmly pushing against the sides of the tub so that hasn't occurred yet, only on the surface.
I suspect as the myc grew and now fully colonized the sub, the heat it emits has increased, resulting in more temperature difference with the outside, hence the crazy condensation. Should also mention I live in a place where RH is always high (~75%) in general, too, limiting evap.
What can I do beyond loosening the poly ? would removing it entirely be fine for even more FAE ?
Edited by Zsuzsi (01/18/21 10:36 AM)
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junk_f00d


Registered: 12/04/15
Posts: 933
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Zsuzsi]
#27155136 - 01/18/21 10:43 AM (3 years, 11 days ago) |
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Quote:
Zsuzsi said: What can I do beyond loosening the poly ? would removing it entirely be fine for even more FAE ?
Slap some paper towels down on the surface to soak that stuff up
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Zsuzsi
Stranger
Registered: 03/05/19
Posts: 86
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Re: Cultivation General Discussion [Re: Zsuzsi]
#27155151 - 01/18/21 10:57 AM (3 years, 11 days ago) |
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Did that already, but it comes back. I'm a tad worried there just isn't enough evap going on to trigger pinning.
I'm also seeing this since about yesterday, on the side and at the bottom:

See these two yellow spots at the center, and the other I've outlined ? they weren't there before, and it fact that area was covered with strong myc. The color is most definitely different from the metabolites I see on the top surface. It only occurs on the sides and at the bottom.
I'm probably just delusional due to my horror at possibly losing what is my first monotub after 2 months since my spore germing on agar, but I could swear it kinda looks like newly formed pins pressing against the plastic. But it makes no sense - there can't be any evaporation there, as the myc hasn't shrinked at all thus far.
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EZPZyaBZ
J u s t V i s i t i n g

Registered: 01/10/21
Posts: 133
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Re: Cultivation General Discussion [Re: Zsuzsi]
#27155173 - 01/18/21 11:09 AM (3 years, 11 days ago) |
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Sorry about your luck man, I feel your pain. Unfortunately it happens to us all when we get/got started. Contamination rears it's ugly head. Many if not most of the tubs I have done that are as you describe were headed for failure. Sounds like the sub is fighting to live, but with that much moisture pooling consistently to that level combined with the other new developments it's best to get some other tubs started while you wait to see how this plays out for sure.
Edited by EZPZyaBZ (01/18/21 11:37 AM)
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: Zsuzsi]
#27155184 - 01/18/21 11:15 AM (3 years, 11 days ago) |
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Quote:
Zsuzsi said: Did that already, but it comes back. I'm a tad worried there just isn't enough evap going on to trigger pinning.
I'm also seeing this since about yesterday, on the side and at the bottom:

See these two yellow spots at the center, and the other I've outlined ? they weren't there before, and it fact that area was covered with strong myc. The color is most definitely different from the metabolites I see on the top surface. It only occurs on the sides and at the bottom.
I'm probably just delusional due to my horror at possibly losing what is my first monotub after 2 months since my spore germing on agar, but I could swear it kinda looks like newly formed pins pressing against the plastic. But it makes no sense - there can't be any evaporation there, as the myc hasn't shrinked at all thus far.
That tub is about to fruit and those are just little blobs pressing against the plastic, it happens sometimes. It may be a reaction to a little bacteria but likely nothing to worry about. A pic of the surface would say more if you can take it.
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GrinchGrower
N00B



Registered: 10/02/20
Posts: 682
Last seen: 2 years, 8 months
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Re: Cultivation General Discussion [Re: Zsuzsi]
#27155195 - 01/18/21 11:20 AM (3 years, 11 days ago) |
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Quote:
Zsuzsi said: the heat it emits has increased, resulting in more temperature difference with the outside, hence the crazy condensation. Should also mention I live in a place where RH is always high (~75%) in general, too, limiting evap.
What can I do beyond loosening the poly ? would removing it entirely be fine for even more FAE ?
Use a fan?
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Zsuzsi
Stranger
Registered: 03/05/19
Posts: 86
Last seen: 6 months, 16 days
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Re: Cultivation General Discussion [Re: Josex]
#27155217 - 01/18/21 11:28 AM (3 years, 11 days ago) |
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Quote:
Josex said: A pic of the surface would say more if you can take it.
I took pics of the surface almost exactly 7 days ago for this thread (I hope linking it instead of duplicating the pictures here is OK in terms of forum etiquette, feel free to tell me if it isn't). At the time there was only one spot of golden metabolites like depicted in the thread, as I said now there are about six, all identical. At the time there were still parts of thinly colonized subs visible through the sides, but now those are mostly well-covered up almost entirely.
The surface looks roughly the same now except for the few extra spots (about the same size, and not at all connected to the initial spot - they're on various parts of the surface, in my mind where pooling water was). I do not see any obvious hyphae except perhaps in like two spots where there's a thin white dot but again, it's probably me being delusional. You really think it'll fruit something ? for what's it's worth even with the new metabolite spots it smells extremely mushroomy but nothing else, not even a hint of something wrong. The spawn jars were the same.
Quote:
EZPZyaBZ said: Sorry about your luck man, I feel your pain [...]
I want to thank you for this comment as it really makes me sad that my tub may end in failure, and this made me feel a little better. I've got the other one at least - it's a bit behind in terms of colonization due to slightly lower temps where it is I think, but at least there isn't any traces of metabolites in this one. It was built exactly the same in terms of holes though, so I now also fear for it.
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Zsuzsi
Stranger
Registered: 03/05/19
Posts: 86
Last seen: 6 months, 16 days
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Re: Cultivation General Discussion [Re: GrinchGrower]
#27155224 - 01/18/21 11:33 AM (3 years, 11 days ago) |
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Quote:
GrinchGrower said: Use a fan?
Close but directed away from the tub, towards a wall or something, right ? I will attempt that. I really wonders how much the constant high RH in my area is fucking me. Though perhaps it was just bad spawn - though I was deeply paranoid both in my sterile technique, agar rejections (threw several plates that were probably fine on the slightest of suspicions - the wedges that went in the jars were T2 from MS, if that matters), and jar sniffing. I think my technique was OK, which makes me all the more sad at the current situation. Perhaps I was careless of the field capacity in my coir - too much may explain so much water ? though I watched like 10 field capacity videos on this very forum and elsewhere to be sure what to look for - I felt pretty sure I had it right.
Edited by Zsuzsi (01/18/21 11:35 AM)
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Wall.E
Bacteria's Bitch



Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
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Re: Cultivation General Discussion [Re: Zsuzsi]
#27155230 - 01/18/21 11:36 AM (3 years, 11 days ago) |
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See man, what you're going through we've all gone through and it's part of the reason I love mycology so much. It forces you to accept failure. If you're gonna keep at it in this hobby at a certain point you just have to accept that shit's fucked and you have to restart. There are so many people that dread going back to spores because well,
1. It's a pain to work through germ plates and clean the mycelium up and yadda.
2. It's having to risk the failure again. Whether the spores don't take, the jars get fucked or you hit the point you're at, or even worse, trich slipped in and took over 5 out of 6 of your shoeboxes overnight (loooool that was fuckin fun).
Thats why when people find cultures they like, they keep em for as long as possible.
But the cool thing is that once you get there, you can do it again, only this time you have more experience.
Good luck man
-------------------- Lifeβs shit, but Iβm loving it
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: Zsuzsi]
#27155241 - 01/18/21 11:41 AM (3 years, 11 days ago) |
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Yea that surface ain't looking pretty to me either, it looks bacterial. Well at least you have another tub looking good, this is all a learning experience for you.
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Zsuzsi
Stranger
Registered: 03/05/19
Posts: 86
Last seen: 6 months, 16 days
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Re: Cultivation General Discussion [Re: Zsuzsi]
#27155246 - 01/18/21 11:43 AM (3 years, 11 days ago) |
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Myc_Hunt, thanks for your comment also. I'm not about to give up :-) just feeling pretty down. It's not my first time producing psychs - I nailed DMT extraction on my first try, for example, but it's child's play compared to this hobby and I love caring for what is an incredibly awesome lifeform and seeing it grow - I feel much more connected to what I produce.
Would appreciate any additional comments on what the surface of the thread linked above look like, too; should I still hold some amount of hope for this tube ? should I try to modify the other - exactly the same in terms of holes, though in a closet - to try and increase FAE ? that one doesn't have metabolites at least, but its slowness in completing colonization worries me.
And part of me also wonders if it might be best letting the other tub grow as-is: because if it fruits, then I'll know pretty much for sure it was likely to be bad spawn in one of the jars that went into the first one. Though like a moron of course I split a single jar between both tubs, so if it was that one I'm fucked.
Edit: also, you'll be happy to know that I won't have to start with fucking spores again (got bit by fucking spores not germinating on my agar once before - so now I have leftover T1 plates in the fridge, and they're only like 6 weeks old so I know they'll transfer again, thank god).
Edited by Zsuzsi (01/18/21 05:50 PM)
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