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adhoc


Registered: 05/03/20
Posts: 167
Last seen: 1 year, 7 months
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Re: Cultivation General Discussion [Re: mushboy]
#27148545 - 01/14/21 07:58 PM (3 years, 15 days ago) |
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Quote:
mushboy said: i prefer human horn
Human horn? But ... it is forbidden!
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Tstone
St.of Circumstance



Registered: 10/16/18
Posts: 944
Loc: This Planet
Last seen: 7 days, 3 hours
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Re: Cultivation General Discussion [Re: junk_f00d]
#27148576 - 01/14/21 08:15 PM (3 years, 15 days ago) |
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Alcohol to loosen up a spore print? What's your normal spore inoculation procedure? They do make sterile swabs and inoculation loops. You actually used iso alcohol to spray a print, to loosen spores. Can't see that outcome to be terribly positive.
-------------------- Wake now, discover that you are the song that morning brings, but the heart has it's seasons, it's evenings and songs of it's own"
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GrinchGrower
N00B



Registered: 10/02/20
Posts: 682
Last seen: 2 years, 8 months
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Re: Cultivation General Discussion [Re: Tstone]
#27148597 - 01/14/21 08:30 PM (3 years, 15 days ago) |
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I'm a n00b just blindly following advice, but my initial reaction was:
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junk_f00d


Registered: 12/04/15
Posts: 933
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Tstone]
#27148673 - 01/14/21 09:12 PM (3 years, 15 days ago) |
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Quote:
Tstone said: Alcohol to loosen up a spore print? What's your normal spore inoculation procedure? They do make sterile swabs and inoculation loops. You actually used iso alcohol to spray a print, to loosen spores. Can't see that outcome to be terribly positive.
My loop is homemade, I flame sterilize it. Just scrape some off and transfer to the plate. If the alcohol doesn't kill the spores, I can't see it being that bad. If it works, it will have just carried less bacteria with it.
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GypsyBastard
Misanthrope


Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
Last seen: 2 years, 10 months
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Re: Cultivation General Discussion [Re: junk_f00d]
#27148730 - 01/14/21 09:48 PM (3 years, 14 days ago) |
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I mean, I'm not gonna lie my gut instinct say that's bad but I also know I that I know fuckall so..... Let me know how it works out! Maybe you're on to something
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mushhead
Potato Devourer



Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
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Re: Cultivation General Discussion [Re: GypsyBastard] 1
#27148769 - 01/14/21 10:21 PM (3 years, 14 days ago) |
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Wall.E
Bacteria's Bitch



Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
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Re: Cultivation General Discussion [Re: mushhead]
#27148971 - 01/15/21 02:28 AM (3 years, 14 days ago) |
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Next time if you're trying to get spores loose use distilled water or scrape them off using a scalpel. Alcohol might not fuck up the spores but there's a reason we use it to clean our tools and gloves.
-------------------- Life’s shit, but I’m loving it
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junk_f00d


Registered: 12/04/15
Posts: 933
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Wall.E]
#27149005 - 01/15/21 02:59 AM (3 years, 14 days ago) |
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Quote:
Myc_Hunt said: Next time if you're trying to get spores loose use distilled water or scrape them off using a scalpel. Alcohol might not fuck up the spores but there's a reason we use it to clean our tools and gloves.
I said earlier I do normally use water on troublesome prints (or some agar gunk), but didn't have any available and so just yolo'd it with my alcohol bottle on my last two plates as an expirement. To be clear I'm not asking for advice on how to dislodge tough prints, but if alcohol will kill the spores or not. If it *does* work, and the spores do germinate in the same timeframe and seem just as healthy, I'd have a) a cleaner germination due to bacteria being pwn'd by the iso transfer and b) the convienence and just using isopropyl alcohol instead of water.
I also have a gut feeling it will not be successful, but it only cost me a couple plates to find out. On second thought, maybe it is a shit idea though, given that my alcohol bottle is going to have tons of spores in it that I'm then carrying to my plate..
Edited by junk_f00d (01/15/21 03:15 AM)
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Wall.E
Bacteria's Bitch



Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
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Re: Cultivation General Discussion [Re: junk_f00d]
#27149021 - 01/15/21 03:28 AM (3 years, 14 days ago) |
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Meh, there's millions of spores. You might be fine. I drench shit in alcohol and still find mold in my plates, wouldn't be surprised if some cube spores survive too
-------------------- Life’s shit, but I’m loving it
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Schemenhaft
Oachkatzlschwoaf



Registered: 01/14/17
Posts: 339
Loc: Right side of the big poo...
Last seen: 2 years, 8 months
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Re: Cultivation General Discussion [Re: Wall.E]
#27149086 - 01/15/21 04:25 AM (3 years, 14 days ago) |
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Do you guys have an idea how to get better temperature for my mushrooms? I have maximum 20degrees in my apartment with the radiators at maximum, freaking cold here these days and not the best isolation.
Don't want to run the radiators that hard, I prefere like 17-18degrees but the mushrooms don't, grow is very slow here. I know putting them in front of the radiator or using heatpads is shit.
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 2 hours, 40 minutes
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Re: Cultivation General Discussion [Re: Schemenhaft] 1
#27149124 - 01/15/21 04:56 AM (3 years, 14 days ago) |
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That should be fine for fruiting. For colonization I’ll put the jars in a box or tub and aim a little heater at it then check the temp until I figure out how far away from the heater holds the right temperature.
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LAGM2020     
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Schemenhaft
Oachkatzlschwoaf



Registered: 01/14/17
Posts: 339
Loc: Right side of the big poo...
Last seen: 2 years, 8 months
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Re: Cultivation General Discussion [Re: A.k.a]
#27149127 - 01/15/21 04:58 AM (3 years, 14 days ago) |
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Whats happening here? This is a transfer from a descent rhizomorphic plate B+. I think it belongs to the agar/lme mixture - This seems to mostly happens If I'll go for a 4g/4g/200ml recipe. Tried recipes with less water and never had this again - But slowed down the growth also. Any ideas?
__________________________
@aka
Yeah fruiting seems to be okay, not as fast as it could be but fine. But colonization, especially in my jars, is very slow If i keep my temperature dat low.
I'll try your tip next week, currently I have like 30 jars colonising, so thats a bit of work.
Edited by Schemenhaft (01/15/21 05:01 AM)
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
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Re: Cultivation General Discussion [Re: Schemenhaft] 1
#27149135 - 01/15/21 05:04 AM (3 years, 14 days ago) |
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Kinda looks like there’s bacteria mixed in the agar or something.
Yeah a few degrees can make a big difference with jars. Cardboard or a plastic tub holds heat pretty well so maybe even putting boxes near the radiator would help. Right now my grow room where I have everything stays around 63f but inside the tub of jars is 70-74f. The jars seriously move like 5 times faster inside the box than outside.
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LAGM2020     
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Schemenhaft
Oachkatzlschwoaf



Registered: 01/14/17
Posts: 339
Loc: Right side of the big poo...
Last seen: 2 years, 8 months
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Re: Cultivation General Discussion [Re: A.k.a]
#27149141 - 01/15/21 05:20 AM (3 years, 14 days ago) |
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Shouldn't be any kind of contamination, this happens like 99% of time at my plates with agar/lme 4g/4g/200ml. All other plates looking fine, even with transfers from same culture.
I really think it belongs to the mixture of agar/lme and the amout of water - If it belongs to contamination, I should get similar results on other plate-mixes right?
Yeah, I have also all jars in Shoeboxes placed on carpets. Think on sunday I have enough 400ml jars to go for g2g inoculation, this should speed up new things very fast.
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The Fresh Prints
Smell ya later



Registered: 05/19/12
Posts: 1,377
Loc: Bel-Air
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Re: Cultivation General Discussion [Re: Schemenhaft]
#27149220 - 01/15/21 07:03 AM (3 years, 14 days ago) |
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Quote:
Schemenhaft said: Do you guys have an idea how to get better temperature for my mushrooms? I have maximum 20degrees in my apartment with the radiators at maximum, freaking cold here these days and not the best isolation.
Don't want to run the radiators that hard, I prefere like 17-18degrees but the mushrooms don't, grow is very slow here. I know putting them in front of the radiator or using heatpads is shit.
I have at least one dehydrator running at any given time in my grow room lol. Seems to raise the temp a little bit.
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clockworkshroom
Stranger


Registered: 02/20/20
Posts: 655
Last seen: 2 years, 7 months
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On the top is a 4th gen transfer of some GT ms, on the bottom the way thicker, crazy looking mofo is a 2nd gen I did at the same time as the 4th as I saw some interesting growth on another section of the very original plate.
I'm going to put some in grain and do g2g and eventually a mono.
Based on your experiences would you continue with the plates I've got to 4th gen on or do further transfers on the other?
Thanks all
Edited by clockworkshroom (01/15/21 07:13 AM)
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BondVillain
Stranger



Registered: 08/14/18
Posts: 118
Last seen: 2 years, 10 months
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I think my BFR cakes are ready to be 'birthed.' I inoculated them on 12/17 so it's been about 28-29 days. My jars have started pinning. Question: what should I do with the pins? Will submerging them in water kill them? Should I remove?
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GrinchGrower
N00B



Registered: 10/02/20
Posts: 682
Last seen: 2 years, 8 months
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Re: Cultivation General Discussion [Re: BondVillain]
#27149495 - 01/15/21 10:17 AM (3 years, 14 days ago) |
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Quote:
BondVillain said: I think my BFR cakes are ready to be 'birthed.' I inoculated them on 12/17 so it's been about 28-29 days. My jars have started pinning. Question: what should I do with the pins? Will submerging them in water kill them? Should I remove?
Just pick or brush them off when you rinse and dunk the cakes.
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Mr. Mushie

Registered: 01/16/20
Posts: 667
Last seen: 2 months, 25 days
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Re: Cultivation General Discussion [Re: GrinchGrower]
#27149502 - 01/15/21 10:28 AM (3 years, 14 days ago) |
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Does anyone sterilize slants in quart jars instead of pints like so?

I see all the teks use pints but I don't see why this wouldn't work.
The only downside being it's harder to reach in and tighten the caps after the PC cycle.
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smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,862
Last seen: 13 days, 7 hours
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Re: Cultivation General Discussion [Re: Mr. Mushie]
#27149527 - 01/15/21 10:42 AM (3 years, 14 days ago) |
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It works but the depth is annoying and finicky
-------------------- Willpower is the one true virtue
  
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