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InvisibleDoctor Mario
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Re: Cultivation General Discussion [Re: Zsuzsi]
    #27127218 - 01/04/21 10:30 PM (3 years, 25 days ago)

Quote:

Zsuzsi said:
Hi all,

I'm having my first two monotubs colonize right now; they're WBS from agar + coir only, at a ratio of 1:2. They're relatively small monotubs (~30 qt).

Anyway, I've noticed that both already have their surface entirely covered (it's been 5 days, no casing), while the interior - I used no liner so I can see it - is taking noticeably longer and most of it thus far is wispy exploratory mycelium. I'm not overly worried as the myc clearly continues to spread even there and I've already got several small patches of solid white myc even there.

Just wondered, however, if it's normal and usual/expected ? kinda makes sense to me as it's easier for the myc to move accross the surface than through the sub but wanted some feedback.




Have any pictures you can share?


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OfflineZsuzsi
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Re: Cultivation General Discussion [Re: Doctor Mario]
    #27127240 - 01/04/21 10:49 PM (3 years, 24 days ago)

Quote:

Doctor Mario said:
Have any pictures you can share?




Of course ! There you go:



That's the side and surface of the first tub. I apologize for the quality / visibility due to condensation, obviously (there's some temperature swings in this one due to its location, hence a bit of condensation on it).

Yesterday I still saw some coir (not very much) on the surface but no longer. The side picture might not show it very well but even between the white myc lumps there is some myc, just much much thinner.


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Offlineclockworkshroom
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Re: Cultivation General Discussion [Re: Zsuzsi]
    #27127704 - 01/05/21 08:16 AM (3 years, 24 days ago)

did you pack it down tightly? it shouldn't be compressed.


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p9hu7's clean spawn thread


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OfflineZsuzsi
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Re: Cultivation General Discussion [Re: clockworkshroom]
    #27128478 - 01/05/21 02:57 PM (3 years, 24 days ago)

I tried to make it airy but yeah, might've a bit to level out the surface.


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OfflineAlsetAlokin
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Re: Cultivation General Discussion [Re: clockworkshroom]
    #27128541 - 01/05/21 03:25 PM (3 years, 24 days ago)

Quote:

clockworkshroom said:
did you pack it down tightly? it shouldn't be compressed.




Just a novice here, but I pat my sub down to level it out and create a tighter surface. It makes picking them a little easier as a loose sub likes to pull up on the pluck for me.

Got any resources why a more compressed sub is detrimental, or could be causing problems here?


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OfflineEugene Gesuale
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Re: Cultivation General Discussion [Re: AlsetAlokin]
    #27128545 - 01/05/21 03:26 PM (3 years, 24 days ago)

Quote:

AlsetAlokin said:
Quote:

clockworkshroom said:
did you pack it down tightly? it shouldn't be compressed.




Just a novice here, but I pat my sub down to level it out and create a tighter surface. It makes picking them a little easier as a loose sub likes to pull up on the pluck for me.

Got any resources why a more compressed sub is detrimental, or could be causing problems here?



Gotta be a sweet spot if I may say so myself. Using verm should allow for more pressure when you tamp I’d assume


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Invisiblemushhead
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Re: Cultivation General Discussion [Re: Eugene Gesuale]
    #27128553 - 01/05/21 03:28 PM (3 years, 24 days ago)

I pack my tubs. I tamp it down. It's snug in there.
amount of pressure used to tamp isn't a factor I don't think.
I'm not rough with the myc, but I don't play around the bush either.
Never linked an issue with how I tamped my subs as long as their leveled.


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OfflineEugene Gesuale
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Re: Cultivation General Discussion [Re: Eugene Gesuale]
    #27128556 - 01/05/21 03:30 PM (3 years, 24 days ago)

I know for me personally, even when I followed a tek to the T, I was always baked or drunk or speeding my tits off on adderall and would hurry through shit or just overlook such simple sterile stuffs. Maybe try thinking like a πŸ„


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OfflineCrackatoa
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Re: Cultivation General Discussion [Re: Eugene Gesuale] * 1
    #27128568 - 01/05/21 03:36 PM (3 years, 24 days ago)

There is a sweet spot, overly tamped down will slow colonization of substrate.


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OfflineZsuzsi
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Re: Cultivation General Discussion [Re: Crackatoa]
    #27128588 - 01/05/21 03:44 PM (3 years, 24 days ago)

Thanks for all your comments - I also assume there is a sweet pot over which compressing it too much is detrimental to myc growth speed (makes sense). I'd bet on temperature being more the culprit in my case though, but then again I'm a noob so that's only a guess.

With all that being said, does my tub look roughly OK ? to avoid the long wait I'm hesitating between starting new grain jars right now from my leftover T2 plates while the tubs mature or waiting for harvest (if it ever comes) to try for a clone.


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OfflineCrackatoa
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Re: Cultivation General Discussion [Re: Zsuzsi]
    #27128613 - 01/05/21 03:56 PM (3 years, 24 days ago)

It's hard to tell without a top view but from that angle it looks good. Did you put a top layer of coir at spawning? I always recommend a top layer.


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OfflineZsuzsi
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Re: Cultivation General Discussion [Re: Crackatoa]
    #27128629 - 01/05/21 04:03 PM (3 years, 24 days ago)

Thanks ! I did not case, there were grains at the top - this is my first monotub so I wanted to avoid absolutely anything that might jeopardize it even remotely. Stuck to the basic approach (will start experimenting with further monotubs). Same reason I didn't open it for the picture - though I know it's not really risky, especially with the whole surface colonized.

Gotta say it went splendidly thus far - I'm amazed at how wonderful the agar approach is. Especially after previously trying 8 PF cakes directly from a MS syringe; 7 of them went bacterial.


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Offlineclockworkshroom
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Re: Cultivation General Discussion [Re: Zsuzsi]
    #27128725 - 01/05/21 04:50 PM (3 years, 24 days ago)

Quote:

AlsetAlokin said:
Quote:

clockworkshroom said:
did you pack it down tightly? it shouldn't be compressed.




Just a novice here, but I pat my sub down to level it out and create a tighter surface. It makes picking them a little easier as a loose sub likes to pull up on the pluck for me.

Got any resources why a more compressed sub is detrimental, or could be causing problems here?




No, purely based on hearing Bod say not to compress it too much in one of his videos.

I'm far too much of a novice to be answering any questions at all but just want to try and contribute as I ask so much myself, so if I see something I've seen covered I try and answer.

When it's not based on my experience I'll make sure I quote source


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Offlineclockworkshroom
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Re: Cultivation General Discussion [Re: Zsuzsi]
    #27128753 - 01/05/21 05:01 PM (3 years, 24 days ago)

Quote:

Zsuzsi said:
Thanks ! I did not case, there were grains at the top - this is my first monotub so I wanted to avoid absolutely anything that might jeopardize it even remotely. Stuck to the basic approach (will start experimenting with further monotubs). Same reason I didn't open it for the picture - though I know it's not really risky, especially with the whole surface colonized.

Gotta say it went splendidly thus far - I'm amazed at how wonderful the agar approach is. Especially after previously trying 8 PF cakes directly from a MS syringe; 7 of them went bacterial.




When did they go bacterial? I have some from ms germinating at the moment, they are about 10-12 days in and look super clean so far. I'm also doing agar on the side ready for monotubs, I did my 4th transfers earlier and am hoping they'll produce the dish I put to grain.

Some of my earlier dishes which my first transfer came from which have a drop of blue food dye in have started to go almost a pinky/brown colour, the agar not the myc. I read that myc can start to eat the dye if left a long time, is this how it would look? The myc still looks clean but don't want it to be a dormant contam that will appear later in my transfer dishes.

Last question, my PF cakes all have rhizo growth but my agar from the same spores is all tomo (8-10g agar agar, 8+10g LME, 500ml water). I know this isn't important but as a newb I'd love to see rhizo on agar. Any ideas why it may be? I know cutting nutrients may promt rhizo but I see lots of people with the same recipe get rhizo. One of my old dishes had visible rhizo right on the border where the agar ended (I guess it was due to lack of nutrition), I've transferred a bit of it to agar today but expect it will go tomo.

Thanks all


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OfflineCrackatoa
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Re: Cultivation General Discussion [Re: Zsuzsi] * 1
    #27128768 - 01/05/21 05:07 PM (3 years, 24 days ago)

Quote:

Zsuzsi said:
Thanks ! I did not case, there were grains at the top - this is my first monotub so I wanted to avoid absolutely anything that might jeopardize it even remotely. Stuck to the basic approach (will start experimenting with further monotubs). Same reason I didn't open it for the picture - though I know it's not really risky, especially with the whole surface colonized.

Gotta say it went splendidly thus far - I'm amazed at how wonderful the agar approach is. Especially after previously trying 8 PF cakes directly from a MS syringe; 7 of them went bacterial.




Casing and top layering are two completely different things. Top layer is just a layer of coir at spawning. Casing is peat and verm and lime put on after colonization.


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OfflineAlsetAlokin
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Re: Cultivation General Discussion [Re: clockworkshroom]
    #27128793 - 01/05/21 05:15 PM (3 years, 24 days ago)

Quote:

clockworkshroom said:

Some of my earlier dishes which my first transfer came from which have a drop of blue food dye in have started to go almost a pinky/brown colour, the agar not the myc. I read that myc can start to eat the dye if left a long time, is this how it would look? The myc still looks clean but don't want it to be a dormant contam that will appear later in my transfer dishes.

Thanks all




Yep, myc eats the dye. That's normal.


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OfflineZsuzsi
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Re: Cultivation General Discussion [Re: clockworkshroom]
    #27128812 - 01/05/21 05:21 PM (3 years, 24 days ago)

Quote:

Crackatoa said:
Top layer is just a layer of coir at spawning.




Thanks, I was wondering if the use of the term "casing" was accurate given it's - relatively - nutritious stuff. What is the advantage of a top nutritious layer like this ?

Quote:

clockworkshroom said:
When did they go bacterial?
[...]
Any ideas why it may be? I know cutting nutrients may promt rhizo




On the first question, no idea - see the post where I asked why here but more generally, I suspect either my sterilization approach or the syringe itself.

Edit: sorry, you asked when, not why: they went bacterial after about a week in the fruiting chamber.

On the second question, no idea if that's true but honestly from MS to agar I've given up on trying to acquire rhizo growth (will do that with a clone) - mine was not rhizo growth, even the T2 going to grain. From what I've read it doesn't really matter or impacts fruiting much when you start from MS.


Edited by Zsuzsi (01/05/21 05:22 PM)


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Offlineclockworkshroom
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Re: Cultivation General Discussion [Re: Zsuzsi] * 1
    #27128820 - 01/05/21 05:22 PM (3 years, 24 days ago)

I'm going to add some visuals to my last post

Healthy looking (to me at least) brf jar


My transfer generations, you can see slight discolouring of the first


The silly amount of dishes I currently have germinating, most will end up being tossed I expect.


The discolouring of an older dish. Contam or myc absorbing dye?



A bag load more of old dishes I need to dispose of. I went a bit over the top but all good practice and basically zero contamination.


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p9hu7's clean spawn thread


Edited by clockworkshroom (01/05/21 05:24 PM)


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Offlinestarbones
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Re: Cultivation General Discussion [Re: clockworkshroom]
    #27128902 - 01/05/21 05:51 PM (3 years, 24 days ago)

I want to see someone top fruit a colonized dish.
I use 8oz pp5 deli containers. I think I might let a thick poured dish colonize then toss an inch or two of CVG on it then throw the lid on with small FAE holes and see what happens.


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OfflineEugene Gesuale
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Re: Cultivation General Discussion [Re: starbones]
    #27128905 - 01/05/21 05:53 PM (3 years, 24 days ago)

I was gonna try this with oats. Still might


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