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Eugene Gesuale
Jar-Sniffer



Registered: 04/12/20
Posts: 1,920
Loc: The Basement
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And I always wait till 5psi before weighting it
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BrownBear
Warrior-Traveler



Registered: 06/05/20
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I have an AA also. I think it would be almost impossible to clamp down the lid on one of those if the water was already boiling. Definitely wouldn't be worth the effort regardless.
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Eugene Gesuale
Jar-Sniffer



Registered: 04/12/20
Posts: 1,920
Loc: The Basement
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Re: Cultivation General Discussion [Re: BrownBear]
#27116010 - 12/30/20 10:55 AM (3 years, 30 days ago) |
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Agreed. Do you ever unwrap your scalpel blade in the sab or flow hood and just skip the flame for the first transfer?
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sh4d0ws
LSx


Registered: 02/26/08
Posts: 12,086
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Quote:
MH5109 said: Hot water, sodium hydroxide, bleach, and dish soap. It may have to sit a little bit but if its oil that will remove it.
You should not put NaOH (sodium hydroxide) in an aluminum pressure cooker
Quote:
Adrenochrome said: Agreed. Do you ever unwrap your scalpel blade in the sab or flow hood and just skip the flame for the first transfer?
After sterilizing in a PC? Yes. I do this all the time.
I know some people don't even bother sterilizing their scalpels but I sterilize mine all the time and the first transfers afterwards I never flame
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Culliganman360
Stranger



Registered: 06/05/19
Posts: 854
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Re: Cultivation General Discussion [Re: sh4d0ws]
#27116044 - 12/30/20 11:14 AM (3 years, 30 days ago) |
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Little late on this but I been getting good results doing 1500 g birdseed to 800 g water. No prep bags I fit 6 in my presto
Edited by Culliganman360 (12/30/20 11:14 AM)
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Eugene Gesuale
Jar-Sniffer



Registered: 04/12/20
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Thanks calligan. I decided to do 150 w to 200 oats per quart.
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BrownBear
Warrior-Traveler



Registered: 06/05/20
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Last seen: 8 months, 7 days
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Quote:
Adrenochrome said: Agreed. Do you ever unwrap your scalpel blade in the sab or flow hood and just skip the flame for the first transfer?
I never pressure sterilize my exacto knife. I just wipe it with iso and flame the blade between transfers.
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Culliganman360
Stranger



Registered: 06/05/19
Posts: 854
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Re: Cultivation General Discussion [Re: BrownBear]
#27116108 - 12/30/20 11:41 AM (3 years, 30 days ago) |
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Anyone know about gas exchange Or gas buildup for a whole room of bags. (Like 3 shelves in a 18x10 )Should I air out the room or something? Noobish question but I don’t wana overlook some simple shit
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tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,206
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Quote:
Culliganman360 said: Anyone know about gas exchange Or gas buildup for a whole room of bags. (Like 3 shelves in a 18x10 )Should I air out the room or something? Noobish question but I don’t wana overlook some simple shit
I'm interested in this too. I bought a cheap CO2 meter and its been fascinating to see how quick buildup happens. My meter tops out at 5,000ppm
Not claiming its important, its just interesting.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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Culliganman360
Stranger



Registered: 06/05/19
Posts: 854
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Re: Cultivation General Discussion [Re: tedoro]
#27116176 - 12/30/20 12:21 PM (3 years, 30 days ago) |
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it just naturally builds at certain times?
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GypsyBastard
Misanthrope


Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
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Re: Cultivation General Discussion [Re: tedoro]
#27116185 - 12/30/20 12:26 PM (3 years, 30 days ago) |
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Re: Scalpel talk a pack of 100 sterile wrapped scalpel blades were $7 or $8 dollars last time I ordered some. Sometimes they even come with a metal handle, but if not they are $3-$5. I would use 1 blade/1-3 sessions or just when I remembered/felt like it. Way cheaper than xactos. I now use them for everything from trimming models to cutting stencils. I'll never buy an xacto blade for anything again.
Re: PC talk I have only even used prestos (i'm poor) but I have never put the lid on after bringing it to boil; always just attached lid, vented, then added weight. I'm curious about this now. Is it for easier/quicker venting? I actually grabbed my manual before posting this to double check I didn't miss something.
Re: CO2 I would love to know more about this as well. I know at least one person that uses oysters for CO2 enrichment in their greenhouse. Unfortunately I have no real info to contribute other than a quip.haha. if you dig deeper into it, can't wait to hear what you find. 

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Eugene Gesuale
Jar-Sniffer



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Posts: 1,920
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Re: Cultivation General Discussion [Re: GypsyBastard]
#27116245 - 12/30/20 12:53 PM (3 years, 30 days ago) |
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So I got the grain done. It seems dry on top. Will the moisture distribute itself or do I need to shake them. I can’t tighten my unmodified lids until they cool. Hmmmm
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mushhead
Potato Devourer



Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
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Just let 'em cool, tighten 'em down, shake 'em up, give them observation time before inoc or keep a control jar that remained sealed. That's what I'd do.
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Eugene Gesuale
Jar-Sniffer



Registered: 04/12/20
Posts: 1,920
Loc: The Basement
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Re: Cultivation General Discussion [Re: mushhead]
#27116279 - 12/30/20 01:04 PM (3 years, 30 days ago) |
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I’m not worried about contams. I’m worried about the dry grain. Will it colonize? Or affect anything once it goes to bulk?
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mushhead
Potato Devourer



Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
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The moisture usually re-distributes within a day after shaken and it should be fine. pics?
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GypsyBastard
Misanthrope


Registered: 11/30/20
Posts: 846
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Re: Cultivation General Discussion [Re: tedoro]
#27116319 - 12/30/20 01:27 PM (3 years, 30 days ago) |
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Quote:
tedoro said:
Quote:
Tell me more about this no prep process if you don't mind. You add boiling water to bags then seal then PC?
I live in a communal building and hate having a ton of smelly grains drying, and I don't own a flowhood, so I need to pre-seal bags. Its been a long road for testing. I think I've prepped wheat and rye a dozen different ways... But I have arrived at pouring the exact amount of boiling water into a myco bag with dry wheat berries and loading four of those into a presto 23. The bags are loose in the PC with lots of extra room. I then bring the PC up to 190f for 3.5 hours. The berries hydrate and take up all the water, without cooking the berries to mush... expanding to fill the PC perfectly. Then I PC the bags for 2.5 hours @250f. At zero PSI I pull the bags and hang them. Noccing them with LI syringes when cool enough. The grains are beautiful, dryish and colonize quickly. I'm making them at 50.5% water content including the water in the dry berries. And its super easy to add or substract a 1/2 percentage point of water content the next go around if you think adjustments are needed. I'll be trying 205f hydrating PC temps next go around to see if I can shave a bit of time off the 3.5 hours of hydrating. It sounds like a lot of time, but its super easy, as you are not moving around loose grains, which is a pain. I'm in a good mood the whole process... I do two PC runs in a day.
That's awesome! Thanks for all the info. I've just started playing around with getting my no prep WBS figured out. I have a small amount of barley I may test out but it's not really enough for more than one run probably. I've used an over night soak and a quick hot soak for WBS but like you said, its just a lot of moving things back and forth and if I can streamline the process a bit, I would love it. Thanks again for the input and info!!

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Eugene Gesuale
Jar-Sniffer



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Re: Cultivation General Discussion [Re: GypsyBastard] 1
#27116333 - 12/30/20 01:30 PM (3 years, 30 days ago) |
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Already seems to be distributed thank you
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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
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Re: Cultivation General Discussion [Re: GypsyBastard]
#27116417 - 12/30/20 02:12 PM (3 years, 30 days ago) |
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Well GB, "Re: PC talk I have only even used prestos (i'm poor) but I have never put the lid on after bringing it to boil; always just attached lid, vented, then added weight. I'm curious about this now. Is it for easier/quicker venting? I actually grabbed my manual before posting this to double check I didn't miss something."
I can't find who I got that idea from. It may be a subtle thing, but what I like is that I can be screwing around and when the water is boiling or close to it I can put the lid on and in a very short time be on the 10 minute steam cycle. As opposed to waiting for it to start blowing steam. Like I said, subtle, but it seems faster also and somehow more in tune with the routine.
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GrinchGrower
N00B



Registered: 10/02/20
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Re: Cultivation General Discussion [Re: Inthepit]
#27116435 - 12/30/20 02:20 PM (3 years, 30 days ago) |
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I started doing the pre-boil before capping my PC.
The only reason I do it is because I have been following Spitball's quick rye prep and wanted to stick with the instructions he linked out to here: Pressure Cooking Basics This is the only reason provided:
Quote:
If you just put the lid and the weight on all at once, the jars will heat unevenly and change your PC time. The pressure and heat will build up before the center of the jars get up to temperature.
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clockworkshroom
Stranger



Registered: 02/20/20
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Re: Cultivation General Discussion [Re: GrinchGrower]
#27116477 - 12/30/20 02:37 PM (3 years, 30 days ago) |
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I have looked in all threads and loads of other sites but still am not sure, is this a contam or just sediment pushed against the jar? It's blackish and in brf. I've inoculated about 15 jars (60 inoculation points) and only see this on one of them.
Either way I've separated the jar and will monitor it but as it's my first time growing I'm typically anxious. I'll stop looking at the jars 40 times a day now also!
Thanks
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