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OfflineLtLurker
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Re: Cultivation General Discussion [Re: Doctor Mario]
    #27112275 - 12/28/20 01:18 PM (3 years, 1 month ago)

IIRC there's a centrifuge technique for getting spores from ape & pe caps. If it's the reliable vendor i'm thinking of, than i think it's worth trying if you don't wanna just trade for a swab.


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: Doctor Mario]
    #27112283 - 12/28/20 01:20 PM (3 years, 1 month ago)

I’d try and get a swab here first.


But even bacterial syringes can work it just takes a long time. A lot of the time if you wait myc will sprout from the bacteria streaks and grow over them into clean areas. But then you gotta do transfers and hope nothing got meshed in real good.


--------------------
LAGM2020


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OfflineSchemenhaft
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Re: Cultivation General Discussion [Re: LtLurker]
    #27112285 - 12/28/20 01:22 PM (3 years, 1 month ago)

So, my Presto 01781 23qt arrived from the US today.

Wanna try it now, following Bodhis TEK step by step, installed everything as in the description, also watched a video to be sure.
Water is rocking heavy now for almost 15 minutes, maybe more - The weight is still off.
In bodhs video the PC locked before the weight was putted on, also there was way more steam coming out of the valve.
My cooker still doesnt want to lock.

Any ideas whats maybe the problem, like any "noob-mistakes"?


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OfflineInthepit
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Re: Cultivation General Discussion [Re: Doctor Mario]
    #27112288 - 12/28/20 01:23 PM (3 years, 1 month ago)

Quote:

Doctor Mario said:
Is it worth trying to germinate APE from a syringe? I usually only see people using swabs. An online vendor has syringes available but I'm not sure I wanna try that. 90% of the pics I see of syringe to agar are plates full of bacteria. I've only ever worked with prints.



Ya go for it Mario and throw yer hat in the LAGM ring!
Hopefully I'm doing a Roarkell APE swab and SW APE syringe.
PM Roarkell


Edited by Inthepit (12/28/20 01:25 PM)


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Offlinejunk_f00d
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Re: Cultivation General Discussion [Re: A.k.a]
    #27112289 - 12/28/20 01:23 PM (3 years, 1 month ago)

when does a multispore culture stop being a multispore culture? e.g, after X transfers it is no longer multispore, or after cloning from a fruit body it is no longer multispore. or does multispore mean a wedge from the same plate the spores germinated on (i.e, no transfers made other than straight to grain)?


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OfflineCamera93
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Re: Cultivation General Discussion [Re: junk_f00d]
    #27112295 - 12/28/20 01:27 PM (3 years, 1 month ago)

I would say a certain vendor syringe is THE way to go
I see a ton of *Not Apes from swabs around here


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: junk_f00d]
    #27112300 - 12/28/20 01:29 PM (3 years, 1 month ago)

You gotta have the heat up high for the presto at first, that seems to help. Just make sure you let steam come out the vent for a few minutes before dropping the weight on. Once you drop the weight then in a minute the lid will lock and start building pressure.


I think ms is just anything not originating from a clone.


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LAGM2020


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OfflineInthepit
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Re: Cultivation General Discussion [Re: Schemenhaft]
    #27112308 - 12/28/20 01:32 PM (3 years, 1 month ago)

Quote:

Schemenhaft said:
So, my Presto 01781 23qt arrived from the US today.

Wanna try it now, following Bodhis TEK step by step, installed everything as in the description, also watched a video to be sure.
Water is rocking heavy now for almost 15 minutes, maybe more - The weight is still off.
In bodhs video the PC locked before the weight was putted on, also there was way more steam coming out of the valve.
My cooker still doesnt want to lock.

Any ideas whats maybe the problem, like any "noob-mistakes"?



I believe you run steam for 10 minutes, then put the weight on, then it'll lock.

Somewhere around here there's an explanation for the 10 minutes, but basically
all the air inside has to be replaced by steam to get hot enough.

               


--------------------
:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:


Edited by Inthepit (12/28/20 01:46 PM)


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Offlineclockworkshroom
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Re: Cultivation General Discussion [Re: Schemenhaft] * 1
    #27112317 - 12/28/20 01:35 PM (3 years, 1 month ago)

Quote:

Schemenhaft said:
So, my Presto 01781 23qt arrived from the US today.

Wanna try it now, following Bodhis TEK step by step, installed everything as in the description, also watched a video to be sure.
Water is rocking heavy now for almost 15 minutes, maybe more - The weight is still off.
In bodhs video the PC locked before the weight was putted on, also there was way more steam coming out of the valve.
My cooker still doesnt want to lock.

Any ideas whats maybe the problem, like any "noob-mistakes"?




Just wait longer, the valve will lift and it you'll have a steady flow of steam. How much water did you put in and is it cold?

If I have a couple of inches of cold water it takes at least 30 minutes for the valve to lift and for it to start venting, then I leave it for 10 mins before adding the weight. On a high heat it then takes about 15 mins to get up to 15psi.

If you want me to film any of the steps I take and post here then I will, just let me know.


--------------------
p9hu7's clean spawn thread


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Offlinejunk_f00d
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Re: Cultivation General Discussion [Re: A.k.a]
    #27112327 - 12/28/20 01:39 PM (3 years, 1 month ago)

Quote:

A.k.a said:
I think ms is just anything not originating from a clone.




well that definition would allow 'MS' cultures to be more isolated than clones, which is counter-productive. for example if you let an MS plate pin and clone it, you now have a non-MS culture but if you do 50 xfers of said plate, letting the myc grow to the edges each time for max cell division / isolation potential, it would still be 'MS' though likely much more 'isolated'.

sorry i know it's a boring question, just been wondering if there was an agreed definition for the term since it's used all the time. i don't really think a clone does anything magical but do think self selection of aggressive genetics will occur as the distance covered by the culture increases (aggressive expanders will expand faster in agar, grain and bulk sub, so by the time bulk fruiting occurs those genetics have dominated more of the sub, so cloning from bulk offers easy access to these genetics but is not the only way or necassarily better and that sufficient selection on agar should result in a culture that is not 'MS').


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OfflineTweeq
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Re: Cultivation General Discussion [Re: clockworkshroom]
    #27112330 - 12/28/20 01:39 PM (3 years, 1 month ago)

Quote:

clockworkshroom said:
Quote:

Schemenhaft said:
So, my Presto 01781 23qt arrived from the US today.

Wanna try it now, following Bodhis TEK step by step, installed everything as in the description, also watched a video to be sure.
Water is rocking heavy now for almost 15 minutes, maybe more - The weight is still off.
In bodhs video the PC locked before the weight was putted on, also there was way more steam coming out of the valve.
My cooker still doesnt want to lock.

Any ideas whats maybe the problem, like any "noob-mistakes"?




Just wait longer, the valve will lift and it you'll have a steady flow of steam. How much water did you put in and is it cold?

If I have a couple of inches of cold water it takes at least 30 minutes for the valve to lift and for it to start venting, then I leave it for 10 mins before adding the weight. On a high heat it then takes about 15 mins to get up to 15psi.

If you want me to film any of the steps I take and post here then I will, just let me know.




I fill it up with 3qts of boiling water from the electric watercooker. Then it will start venting way sooner


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InvisibleDoctor Mario
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Registered: 08/07/20
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #27112337 - 12/28/20 01:42 PM (3 years, 1 month ago)

Quote:

p9hu7 said:
Depends on the vendor, go for it.




That's what I needed to hear. Thanks dude.


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Offlineclockworkshroom
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Re: Cultivation General Discussion [Re: Tweeq]
    #27112338 - 12/28/20 01:42 PM (3 years, 1 month ago)

Yeah I have used boiling water too, depends if I'm in a rush


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OfflineInthepit
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Re: Cultivation General Discussion [Re: clockworkshroom]
    #27112362 - 12/28/20 01:52 PM (3 years, 1 month ago)

Natedawg said he leaves the lid off getting to a boil.
I tried that and it does seem faster. Once it's boiling or
close to it, I put the lid on and in no time it's on the
10 minute steam cycle.


--------------------
:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:


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Offlinestarbones
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Re: Cultivation General Discussion [Re: Inthepit]
    #27112391 - 12/28/20 02:01 PM (3 years, 1 month ago)

Edit nevermind found it


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Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.




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OfflineSchemenhaft
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Re: Cultivation General Discussion [Re: Inthepit]
    #27112408 - 12/28/20 02:08 PM (3 years, 1 month ago)

Quote:

Inthepit said:
Quote:

Schemenhaft said:
So, my Presto 01781 23qt arrived from the US today.

Wanna try it now, following Bodhis TEK step by step, installed everything as in the description, also watched a video to be sure.
Water is rocking heavy now for almost 15 minutes, maybe more - The weight is still off.
In bodhs video the PC locked before the weight was putted on, also there was way more steam coming out of the valve.
My cooker still doesnt want to lock.

Any ideas whats maybe the problem, like any "noob-mistakes"?



I believe you run steam for 10 minutes, then put the weight on, then it'll lock.

Somewhere around here there's an explanation for the 10 minutes, but basically
all the air inside has to be replaced by steam to get hot enough.

               





It was rocking for more then 25 minutes now without a huge steam realease or any locking.
In bodhisattas Video to the exact same PC he had pressure, a locked canner and lots of steam coming out of the valve.

Found here on shroomery that sometimes the lock just won't get up by reason, so possible to fix it with just prying it up.
Did this, putted on the weight and waited to 10 minutes.
Still 0 pressure, the needle made nothing since setting the cooker up.

Decided to take the pressure off now, Reinstalled everything, oiled the rubber seal and I'll give it a second try.

I understood the PC-TEK from bodh, but my PC is not building up pressure.
Or am I maybe impatient? Not sure, but in Bodhs video the pressure increases fast after putting the weight on in just around 3 minutes (there is a clock in the background of the video.


@clockworkshroom

Thanks for your help, there is a video by bodhisatta filming everything.
The point why I am unsure is that he had the lock on, rising pressure and a huge amount of steam coming out before he puts the weight on.
Here is the tek/video in his post.

I used 3l(a bit more then 3qt) hot tap-water, as recommended - But water was rocking like hell for minimum 15 minutes, PC running for over 30 mins all in all.
I installed everything again now and give it a new try with way more time, maybe just differences to the video from time to time, hope it works now.


--------------------


Edited by Schemenhaft (12/28/20 02:18 PM)


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OfflineInthepit
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Re: Cultivation General Discussion [Re: Schemenhaft]
    #27112421 - 12/28/20 02:14 PM (3 years, 1 month ago)

Does the lock move freely with the lid off?
Is your PC on a regular stove with enough heat?


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OfflineSchemenhaft
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Re: Cultivation General Discussion [Re: Inthepit]
    #27112429 - 12/28/20 02:21 PM (3 years, 1 month ago)

Quote:

Inthepit said:
Does the lock move freely with the lid off?
Is your PC on a regular stove with enough heat?




Yes, 100% freeely, if i turn the lid around, the  lock also bumps.

Update: This time it locked after not even 10 minutes of cooking.
Seems like it was just bugging as people wrote sometimes.

Steam is coming out now, not as much as in bodhs video but when its locked I think its fine.
I'll give it not minimum 10 minutes and then put the weight on.

Is it usual, that there is 0 pressure without the weight on?
Seems to be different in bodhs video


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OfflineInthepit
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Re: Cultivation General Discussion [Re: Schemenhaft]
    #27112432 - 12/28/20 02:24 PM (3 years, 1 month ago)

Ya well if yer not on some outrageous burner pressure
can't build with the weight off.

Cool video from Bodhi. Don't forget the vinegar.
I take stuff out as soon the the lock plinks!


Edited by Inthepit (12/28/20 02:36 PM)


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: Inthepit]
    #27112436 - 12/28/20 02:27 PM (3 years, 1 month ago)

I’ll sometimes have like 2psi during venting if I wait too long.


So weird Europe doesn’t have PCs.


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LAGM2020


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