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OfflineGypsyBastard
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Registered: 11/30/20
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Re: Cultivation General Discussion [Re: Sankhara]
    #27110337 - 12/27/20 08:53 AM (3 years, 1 month ago)

Quote:

Sankhara said:
Gypsy i'd really advice you go with a water tub, its much simpler and easier to set up and you wont waste coir. Both tubs work with the exact same machanics




Fair enough. Much appreciated! :cheers:
:mushroom: :heart:


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Offlinesmalltalk_canceled
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Re: Cultivation General Discussion [Re: GypsyBastard]
    #27110470 - 12/27/20 10:15 AM (3 years, 1 month ago)

Great posts on PE, shroomery.

Anyone here run "Huatla" or Columbia?


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Willpower is the one true virtue



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OfflineSchemenhaft
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Re: Cultivation General Discussion [Re: smalltalk_canceled]
    #27110543 - 12/27/20 11:00 AM (3 years, 1 month ago)

So, I shook aroud 48h ago jar of rye with Agar-injection at 90-95% I would say - Could need a second or more opinion about its current situation.
It was placed the last 12 days between the radiator and the wall, thats why we have condensation on the "wall"-side.
I know, that makes it more difficult to say if its bacterial, but would be nice if you guys take a look and tell me what you think.
Do u think its ready to spawn? Wanna try to make mini-cakes or something just4fun if its fine.

This jar is one of my "trial and error" jars, where I try different stuff to see whats happening, so maybe not everything was optimal.




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InvisiblefahtsterM
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Re: Cultivation General Discussion [Re: Schemenhaft] * 1
    #27110568 - 12/27/20 11:17 AM (3 years, 1 month ago)

What’s the timeframe between pics?  Are they all of the same pic session?  The first pic looks less colonized than the last pic. 

I’m confused by your wording.. did you shake when the jar was 90-95% colonized or was the agar that much colonized? 

How cold is your place that you have to put it by a radiator?  Heating jars usually never works out well for me.. room temp, whatever that may be is usually best. 

Definitely looks wet and probably bacterial but some answers to this questions above might help assess better

Faht


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OfflineSchemenhaft
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Re: Cultivation General Discussion [Re: fahtster]
    #27110578 - 12/27/20 11:29 AM (3 years, 1 month ago)

Quote:

fahtster said:
What’s the timeframe between pics?  Are they all of the same pic session?  The first pic looks less colonized than the last pic. 

I’m confused by your wording.. did you shake when the jar was 90-95% colonized or was the agar that much colonized? 

How cold is your place that you have to put it by a radiator?  Heating jars usually never works out well for me.. room temp, whatever that may be is usually best. 

Definitely looks wet and probably bacterial but some answers to this questions above might help assess better

Faht





Pics were all from like 30mins ago - First pic is the side which was turned to the radiator, maybe a reason?
If I compare those pics I see what you mean but in reality all spots looking colonized on the same level more or less - Maybe just the pic giving it a bad view.

Shook the jar when it was at around 90-95% - Maybe a bit less, don't know how it was looking in the inner part of the jar.

Its not like I need to put them to the radiator, did it just to see what happens - How the grow-speed increases and maybe other results.
Grow-speed increased crazy, this jar was crazy fast in relation to the others which are just at maximum 22°C during the day.
During night it drops to around 16-18°C because radiator turns of for 6h.


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Offlinejcm4620
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Re: Cultivation General Discussion [Re: Schemenhaft]
    #27110591 - 12/27/20 11:37 AM (3 years, 1 month ago)

if i shake a jar i do so at the 25-50% range that way it has plenty of time to fully recover for when i break it up agian to spawn. otherwise u risk more of a chance at having it recover weak and either not recovering at all or allowing a contam to over power and take hold. its not good practice to shake or break them up that close of a time frame to eachother is what im getting at


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PANAEOLUS FRUITING MADE SIMPLE



Edited by jcm4620 (12/27/20 11:38 AM)


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OfflineSchemenhaft
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Re: Cultivation General Discussion [Re: jcm4620]
    #27110682 - 12/27/20 01:10 PM (3 years, 1 month ago)

Quote:

jcm4620 said:
if i shake a jar i do so at the 25-50% range that way it has plenty of time to fully recover for when i break it up agian to spawn. otherwise u risk more of a chance at having it recover weak and either not recovering at all or allowing a contam to over power and take hold. its not good practice to shake or break them up that close of a time frame to eachother is what im getting at





Thought we shake at around 30% and around 90%?


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Offlineclockworkshroom
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Re: Cultivation General Discussion [Re: Schemenhaft] * 1
    #27110822 - 12/27/20 02:57 PM (3 years, 1 month ago)

My first ever agar seems to be going ok.

Below are my generations, the ms plate I started with at the front (inoculated 12/12), the 4 first gen transfers in the second row (12/20) and I've just done 5 second gen for each first gen (20 in total) on the back row. Is this excessive? My plan is to pick the best of the second gen and do a few transfers for each one and then probably at least once more so 4 transfers in total before going to grain if it looks uniform. Does that sound reasonable?

There is sign of contam in the original ms plate now, this only just appeared, I assume I shouldn't worry about this? It is away from where I initially transferred.

Do the areas I transferred from look roughly ok? I know the slices are ugly and bigger than ideal but I've never used a scalpel or done agar work before, it will get better.

Thanks to everyone who's helped along the way



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p9hu7's clean spawn thread


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OfflineAlsetAlokin
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Re: Cultivation General Discussion [Re: clockworkshroom]
    #27111017 - 12/27/20 05:00 PM (3 years, 1 month ago)

Quote:

clockworkshroom said:
4 transfers in total before going to grain if it looks uniform. Does that sound reasonable?

I assume I shouldn't worry about this? It is away from where I initially transferred.

Do the areas I transferred from look roughly ok?





Yep. By t4 you will likely be confident that you have a clean culture. Sometimes as early as t2.

Best not to sweat it. Germ plates are likely going to have some contamination. This is why transfers are encouraged.

Areas look good, size looks good, and this is usually around how much I let my t0-t2 grow out before transferring as well.

I'd recommend not using as many plates or making as many transfers, because most MS plates make it to the landfill but it's great practice for now.

My sequence for MS looks like
Germ-2 plates > t1-4 plates > t2-4 plates > t3-2 plates and then hopefully to grain from there

This let's you get to clean MS genetics for half a sleeve of dishes in about 6 weeks.


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Offlineclockworkshroom
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Re: Cultivation General Discussion [Re: AlsetAlokin]
    #27111030 - 12/27/20 05:10 PM (3 years, 1 month ago)

Thanks, appreciate it. Yes definitely wouldn't typically do so many plates but I got a bit trigger happy and had like 40ml GT ms solution, 240 sterilised dishes and lots of time so thought practice would be good. I also have 15 pf tek jars germinating so I can fruit something before my agar work is ready to go to grain :smile: definite overkill

I have a major condensation issue with plates, doesn't seem to impact growth but it's annoying, I'm pouring at 122f or less, was more like 115f today and stack in 10s but still a majority are steaming up. I'm 1 by 1 clearing them with a hot beaker on top at the moment.

It might be temp of the dishes themselves, room temp is around 68f so might try heating the dishes a bit before my next run.


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p9hu7's clean spawn thread


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OnlineCocaineBuffet
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Re: Cultivation General Discussion [Re: AlsetAlokin]
    #27111227 - 12/27/20 07:25 PM (3 years, 1 month ago)

Quote:

AlsetAlokin said:
Quote:

clockworkshroom said:
4 transfers in total before going to grain if it looks uniform. Does that sound reasonable?

I assume I shouldn't worry about this? It is away from where I initially transferred.

Do the areas I transferred from look roughly ok?





Yep. By t4 you will likely be confident that you have a clean culture. Sometimes as early as t2.





That is not a good rule of thumb, all the variant factors are at play until you can identify healthy growth.

There are some prints/swabs/syringes that need further transfers. The transfers could have been done poorly or in poor conditions, the spores might be more dirty than others. In an ideal world that would be the be the case, but dealing with MS from who knows where you can't say with confidence that every plate in every condition will be ready by T4.


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OfflineSchemenhaft
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Re: Cultivation General Discussion [Re: CocaineBuffet]
    #27111278 - 12/27/20 07:56 PM (3 years, 1 month ago)

Harvested the first flush of my Golden Teacher Growkit-cake, which is growing usually in a mini-mono with unmodified tub.
Got enough prints and clones from some decent shrooms, so I modified the box now with Pastys Monotub-Tek without Poly/MP-Tape, because I got only crazy fat short shrooms.
Maybe it was the unmod-tub, maybe another condition, thats what I wanna find out.

Should I "roll" the cake in 2-4mm Vermiculite after soaking or not?
Currently I dont have bigger Verm. because I use usual MonoTubs, is it still worth?


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OfflineSankhara
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Re: Cultivation General Discussion [Re: Schemenhaft]
    #27111786 - 12/28/20 07:54 AM (3 years, 1 month ago)

Is it too late to print from a cap that started sporulating 24h ago?

I see the massive amount of spores that cap dropped and my inexperience tells me its too late for printing

These are the fruits:


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Offlineroarkell
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Re: Cultivation General Discussion [Re: Sankhara]
    #27111790 - 12/28/20 07:56 AM (3 years, 1 month ago)

You'd be surprised... ive had caps that have dropped spores long after I thought they were done...


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OfflineSankhara
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Re: Cultivation General Discussion [Re: roarkell]
    #27111799 - 12/28/20 08:04 AM (3 years, 1 month ago)

Its print time then!


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Invisiblejoze
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Re: Cultivation General Discussion [Re: Sankhara]
    #27111802 - 12/28/20 08:06 AM (3 years, 1 month ago)



Fuck guys is this trich? A couple green/blue spots, about the size of a grain of rice. 6qt shoebox, rye to coir spawned 11 days ago.


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InvisiblefahtsterM
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Re: Cultivation General Discussion [Re: joze]
    #27111807 - 12/28/20 08:09 AM (3 years, 1 month ago)

Not sure if it’s trich but looks like mold for sure


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Invisiblejoze
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Re: Cultivation General Discussion [Re: fahtster]
    #27111811 - 12/28/20 08:13 AM (3 years, 1 month ago)

Quote:

fahtster said:
Not sure if it’s trich but looks like mold for sure




God damn. Dumping it in the trash as we speak. My other shoebox I spawned that day doesn't have any visible mold, fingers crossed I guess.


--------------------


Important Links.

Be kind to others.


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InvisibleDoctor Mario
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Re: Cultivation General Discussion [Re: joze]
    #27112271 - 12/28/20 01:16 PM (3 years, 1 month ago)

Is it worth trying to germinate APE from a syringe? I usually only see people using swabs. An online vendor has syringes available but I'm not sure I wanna try that. 90% of the pics I see of syringe to agar are plates full of bacteria. I've only ever worked with prints.


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: Doctor Mario]
    #27112273 - 12/28/20 01:17 PM (3 years, 1 month ago)

Depends on the vendor, go for it.


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