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starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 18 days
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Re: Cultivation General Discussion [Re: GypsyBastard]
#27102371 - 12/22/20 11:16 AM (3 years, 1 month ago) |
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I'm adding the two quarts of verm to bring the total substrate to 10 quarts worth then dividing it by 10 but I see I already fucked that up. It should be 650g+220g for a total of 870g then divided by 10 to get a weight of 87g per quart then further reduced because eat of my muda style bottles only uses 3/4 so 65.5g per muda style bottle then 150g dry WBS per bottle for a total of 215.5g dry inputs per bottle to extract 25-30g dry or ~300+ wet.
If in a perfect world all bottles made it to the third flush and combined they sat around 300-310g which I'm achieving right now which gives me an oz, sometimes over sometimes under then one PC run makes me 30 muda bottles doing this method or a ~30oz dry yield per PC run if everything was optimal and nothing got lost to contamination. Sometimes the bottles yield far more, sometimes less because the Averys I'm using is not an isolate and things can vary.
Still. That's 1.5-2.0lbs dry fruits per PC run so I think the BE must be decent here? I know my dehydrator is slammed full everyday here now far beyond what I was achieving with my monotubs before. Goal at the time for the monos was 1oz per mycoquart so 10oz nominal from one PC run, feels like I've tripled my efficiency.
-------------------- Listen, I'm steel fisted with the iron lung Heavy metal ballads out the guitar where lions run.
 
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junk_f00d


Registered: 12/04/15
Posts: 933
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: MLPismyOPSEC]
#27102396 - 12/22/20 11:35 AM (3 years, 1 month ago) |
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Quote:
MLPismyOPSEC said: All of them contaming is very concerning! I do have the same issue where plates that sit empty for 6 weeks+ develop contams, but it's at a rate of ~30%. Plates that were poured at the same time but inoculated do not contam at all which i find weird. I wrap the empty plates (using Glad cling wrap) basically as soon as the agar cools, and i get normal/light amounts of condensation. When you wrap, do you try to keep the plate as horizontally level as you can? That's the big thing i pay attention to, and my contam rate of inoculated plates is <1% but blank plates ~30%. (Another side note, this data was all from my SAB days. Got a hood a month ago, have some new empty plates sitting so we will see if the hood changed anything)
Yep, that's all I focus on while wrapping is keeping it horizontal. But I worry that my parafilm is filthy, so I'm just smothering contams on the outside of my plate tha may find their way into the dish after long enough. It is super weird though, I used to toss my plates because they dried up, now they don't even last that long.
I'm about to bust out my SAB if I keep having problems.
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junk_f00d


Registered: 12/04/15
Posts: 933
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: junk_f00d]
#27102401 - 12/22/20 11:37 AM (3 years, 1 month ago) |
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best tek for bags? mine keep coming out with shitty seals, I've watched a few youtube videos but haven't seem to have had the same luck as them. I only get 100% good seals if I tape the bags before PC'ing. ideally with knowledge nuggets like "cool your PC down slowly" and other tips.
this is how I do mine currently: t=594
Edited by junk_f00d (12/22/20 11:43 AM)
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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 14 days, 4 hours
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Re: Cultivation General Discussion [Re: GypsyBastard]
#27102406 - 12/22/20 11:41 AM (3 years, 1 month ago) |
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Quote:
GypsyBastard said:
A/G? Its in here somewhere but no I did not save the convo for future reference. 
 
a/g = anything Well, even if I felt comfortable tightening the media cap before PC, I think I wouldn't like the amount of vacuum generated.
MLPismyOPSEC QFT! ?
Edited by Inthepit (12/22/20 11:44 AM)
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BrownBear
Warrior-Traveler



Registered: 06/05/20
Posts: 1,539
Last seen: 8 months, 7 days
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Re: Cultivation General Discussion [Re: junk_f00d]
#27102413 - 12/22/20 11:46 AM (3 years, 1 month ago) |
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I sanitize the scissors I use to cut the parafilm with iso alcohol. When I peal the parafilm off of the paper, I expose the previously unexposed side only to the laminar flow. Then I wrap each plate with the side that was only exposed to the laminar flow. I have seen less than six contaminated plates out of the 300+ plates I have poured over the past few months. I'd be willing to bet that those contaminated plates came from poor inoculation technique or improper handling.
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junk_f00d


Registered: 12/04/15
Posts: 933
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: BrownBear]
#27102425 - 12/22/20 11:55 AM (3 years, 1 month ago) |
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Quote:
Iambrownbear said: I sanitize the scissors I use to cut the parafilm with iso alcohol. When I peal the parafilm off of the paper, I expose the previously unexposed side only to the laminar flow. Then I wrap each plate with the side that was only exposed to the laminar flow. I have seen less than six contaminated plates out of the 300+ plates I have poured over the past few months. I'd be willing to bet that those contaminated plates came from poor inoculation technique or improper handling.
OK, great. I'll start doing that then, I always had a nagging feeling that could be contributing but I haven't seen it mentioned or done before. Though I doubt that's my biggest problem, because I've always wrapped the same way, but hopefully it will help.
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BrownBear
Warrior-Traveler



Registered: 06/05/20
Posts: 1,539
Last seen: 8 months, 7 days
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Re: Cultivation General Discussion [Re: junk_f00d]
#27102438 - 12/22/20 12:04 PM (3 years, 1 month ago) |
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I'm not sure if that is similar to what everyone else does but it has worked well for me. I have had plates that were a month or older waiting for old spores to germinate without ever seeing a contamination.
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junk_f00d


Registered: 12/04/15
Posts: 933
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: junk_f00d]
#27102452 - 12/22/20 12:14 PM (3 years, 1 month ago) |
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Edited by junk_f00d (12/22/20 12:21 PM)
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junk_f00d


Registered: 12/04/15
Posts: 933
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: junk_f00d]
#27102462 - 12/22/20 12:23 PM (3 years, 1 month ago) |
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what is the ideal RPM for a liquid culture plate stirrer? looking at buying some of these low rpm motors: https://www.aliexpress.com/item/33059907659.html
i think the 28rpm might be best?
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GypsyBastard
Misanthrope


Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
Last seen: 2 years, 10 months
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Re: Cultivation General Discussion [Re: junk_f00d]
#27102480 - 12/22/20 12:36 PM (3 years, 1 month ago) |
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About to pour a bunch of plates for the first time in quite some time. I've missed doing lab work but I kinda hate my current location for it.... Nonetheless, LAGM2021 is quickly approaching and I have a metric fuck ton of spores to get going again. So time to glove up, put on some Thelonious Monk, and do the damn thing, do the damn thing, do it.
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Schemenhaft
Oachkatzlschwoaf



Registered: 01/14/17
Posts: 339
Loc: Right side of the big poo...
Last seen: 2 years, 8 months
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Re: Cultivation General Discussion [Re: GypsyBastard]
#27102666 - 12/22/20 03:24 PM (3 years, 1 month ago) |
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Got a little question about agar.
Had huge problems with condensation, seems like I fixed it with storing the plates in a bigger plastic-box. But now I have the problem that I have a slight film of water at the top of the agar which is flowing around if I pick up the plates.
Till now no big deal, no contaminations or something - But I have on many plates now more and more new spots of cubensis-mycelium starting to grow. Seems like the water is just taking tiny parts of the mycelium and it starts to grow somewhere else then.
Could I fix it with just using less water in my agar recipe ? Currently I do 4g Agar, 4g malt extract and 200ml of water. Any other tips? To which amount of water i could go down for?
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clockworkshroom
Stranger



Registered: 02/20/20
Posts: 655
Last seen: 2 years, 7 months
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Re: Cultivation General Discussion [Re: Schemenhaft]
#27102670 - 12/22/20 03:26 PM (3 years, 1 month ago) |
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would a bag of racehorse oats that have been sat unopened for like 6 months still be good to use?
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smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,862
Last seen: 13 days, 12 hours
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Fatality!
Pearl oysters 1 king Oyster 0

(Mixed tub by mistake, also perlite kill me)
-------------------- Willpower is the one true virtue
  
Edited by smalltalk_canceled (12/22/20 06:52 PM)
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mushhead
Potato Devourer



Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
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That's Beautiful Smalltalk, take a look at my first attempt!
   All just off the one block. This was done with Pearls, food grade hardwood smoking pellets mostly maple was used for the bag, along with some grain spawn and bran husk. It's turning out a lot better than I expected.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: mushhead]
#27102819 - 12/22/20 05:26 PM (3 years, 1 month ago) |
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mushhead
Potato Devourer



Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27102821 - 12/22/20 05:27 PM (3 years, 1 month ago) |
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whatever you say.
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Sankhara
Trump's lost child


Registered: 02/11/18
Posts: 546
Loc: Argentina
Last seen: 10 days, 15 hours
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Re: Cultivation General Discussion [Re: mushhead]
#27102847 - 12/22/20 05:45 PM (3 years, 1 month ago) |
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Quote:
mushhead said:

That looks like the monsters in my darkest nightmares
-------------------- How would you rate the quality of my answer?
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mushhead
Potato Devourer



Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
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Re: Cultivation General Discussion [Re: Sankhara]
#27102854 - 12/22/20 05:47 PM (3 years, 1 month ago) |
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Quote:
Sankhara said:
Quote:
mushhead said:

That looks like the monsters in my darkest nightmares
They haven't reached full size yet lol, but yes cthulhu-like
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: mushhead]
#27102857 - 12/22/20 05:51 PM (3 years, 1 month ago) |
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Those Quote:
mushhead said:
whatever you say.
Those Pleurotus are starving for oxygen. They're not doing well. This is what a healthy pleurotus should look like.
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mushhead
Potato Devourer



Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27102858 - 12/22/20 05:51 PM (3 years, 1 month ago) |
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Quote:
p9hu7 said: Those Quote:
mushhead said:
whatever you say.
Those Pleurotus are starving for oxygen. They're not doing well. This is what a healthy pleurotus should look like.

You could have said that on your first post m8  Just means I need to try harder next time.
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