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GrinchGrower
N00B



Registered: 10/02/20
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Re: Cultivation General Discussion [Re: GypsyBastard] 1
#27101876 - 12/22/20 12:09 AM (3 years, 1 month ago) |
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Hopefully bodhisatta will chime in if he has more info on letting it cool vs. just reaching 0 psi. I'm just not sure if it is a real concern or not...
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RhYzo
D1g1t@l Dw3ll3r



Registered: 01/30/19
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Re: Cultivation General Discussion [Re: GypsyBastard]
#27101886 - 12/22/20 12:29 AM (3 years, 1 month ago) |
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@gypsybastard
It was a new liner tek posted, but no matter I'll play around and see what works for me. I just haven't seen anyone say they have reused them before until now.
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clockworkshroom
Stranger



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Re: Cultivation General Discussion [Re: RhYzo]
#27101939 - 12/22/20 02:44 AM (3 years, 1 month ago) |
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Does this look too fuzzy for mycelium? Super white but it's growing up off the transferred agar like a head of hair.
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Phony Phone

Registered: 09/19/20
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looks good. let it grow out
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clockworkshroom
Stranger



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Re: Cultivation General Discussion [Re: Phony Phone]
#27101951 - 12/22/20 02:58 AM (3 years, 1 month ago) |
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As the growth is mainly on the top of the transferred agar I expect I fucked up and didn't drop it good side down (first ever transfers with a scalpel), will it still be ok?
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Phony Phone

Registered: 09/19/20
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Mine have all fuzzed up at first like this, IME the mycelium cells growing on the plate that was transferred dont grow themselves, instead it fuzzes up and produces new growth on the transferred plate.
For that reason, dont worry about it not being face down on agar. It will colonize.
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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
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Re: Cultivation General Discussion [Re: GrinchGrower]
#27101962 - 12/22/20 03:18 AM (3 years, 1 month ago) |
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Quote:
GrinchGrower said: Hopefully bodhisatta will chime in if he has more info on letting it cool vs. just reaching 0 psi. I'm just not sure if it is a real concern or not...
IME the media jar sucks air BIG TIME when opening in the SAB. Somewhere I got the idea to pull the media jar from the PC as soon as the valve drops, 0 psi. And tighten the cap. The jar is plenty hot still. Soooo, the air inside is still expanded.
Soooo, which is better,
1. pull the media jar quickly and deal with "the suck" inna SAB or Flow? 2. let the jar cool to 120F or so in the PC so there's little "suck".
This, it seems, requires a foil wrap on the cap either way. The theory of tightening the cap before PC sucks even more.
Edited by Inthepit (12/22/20 03:33 AM)
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Sankhara
Trump's lost child


Registered: 02/11/18
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Re: Cultivation General Discussion [Re: Inthepit]
#27101995 - 12/22/20 04:37 AM (3 years, 1 month ago) |
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Why not modify the lid with sfd if you are so worried?
Having it suck air in the SAB shouldnt at all be a problem though.
When i poured my plates before using no pours i used to do that
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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
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Re: Cultivation General Discussion [Re: GypsyBastard]
#27102048 - 12/22/20 06:02 AM (3 years, 1 month ago) |
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Quote:
GypsyBastard said: HOWEVER, This was all done before learning that true media bottles with GL45 lids are designed to vent themselves during sterilization so that is NO NEED to have them cracked in the first place. Just tighten them down, they vent at temp/pressure, then seal as the cool. Easy peasy
Hi GypsyBastard, can you give a reference for that? I can't seem to find a/g here or in DuckDuckGo...thanks 
And Sankhara, the only autocontams I get, usually, are edge contams. So I think the agar itself is not getting contammed. It's just the "suck" that scares me, I mean I could hear it in the SAB! Of course I had foil on but, LOL, it still sucked!
Edited by Inthepit (12/22/20 06:05 AM)
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Sankhara
Trump's lost child


Registered: 02/11/18
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Re: Cultivation General Discussion [Re: Inthepit]
#27102061 - 12/22/20 06:18 AM (3 years, 1 month ago) |
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Nah its nothing to worry about for sure!
_____________________________
I have a question to you all. Is it possible for condensation to form around the area of the transfers if the agar was still slightly warm?
Almost al the plates i did transfers from have that same look, I dont know if it is bacterial contamination or condensation. Its hard to see because of the condensation on those plates, if it werent i would've uploaded a picture
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Phony Phone

Registered: 09/19/20
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Re: Cultivation General Discussion [Re: Sankhara]
#27102084 - 12/22/20 06:53 AM (3 years, 1 month ago) |
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If there was a temp differential, for sure it's possible that condensation will form. But do upload a pic. It would be intresting to see. You do transfers on warm agar?
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Sankhara
Trump's lost child


Registered: 02/11/18
Posts: 546
Loc: Argentina
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Re: Cultivation General Discussion [Re: Phony Phone]
#27102090 - 12/22/20 06:58 AM (3 years, 1 month ago) |
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I would upload one but its reallly difficult to see due to condensation. Thats why im asking. I did transfers as soon as my no pours solidified and they did were a little bit warm.
I'll wait a few days to see if condensation disipates enough to at least take a picture
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Yeetusdeetus



Registered: 11/23/19
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Re: Cultivation General Discussion [Re: Sankhara]
#27102091 - 12/22/20 07:01 AM (3 years, 1 month ago) |
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I think it’s pretty common for no pour to have condensation for a couple days post pc, it is with mini rounds at least
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Phony Phone

Registered: 09/19/20
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Re: Cultivation General Discussion [Re: Yeetusdeetus]
#27102098 - 12/22/20 07:09 AM (3 years, 1 month ago) |
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Yeah, I doubt its bacteria, esp if a temp differential existed. Condensation will form. Wait it out a few days for it to break up into larger droplets, then you will be able to see. But I wouldn't worry.
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0ptiquest
Stranger


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Re: Cultivation General Discussion [Re: Phony Phone]
#27102100 - 12/22/20 07:11 AM (3 years, 1 month ago) |
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Hi there, I want to grow king oysters but i dont have any hardwood sawdust so I found this video.
It explain i can use Supplemented straw with rice bran, i have wheat bran but i hope it works.
My question is how would you make the bags, how i get the supplemented straw to the right moisture content?
Thanks!
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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
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Re: Cultivation General Discussion [Re: Sankhara]
#27102107 - 12/22/20 07:21 AM (3 years, 1 month ago) |
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Quote:
Sankhara said: I would upload one but its reallly difficult to see due to condensation. Thats why im asking. I did transfers as soon as my no pours solidified and they did were a little bit warm.
I'll wait a few days to see if condensation disipates enough to at least take a picture
Since I've been keeping the plates in a dresser drawer condensation has disappeared. It only returns when I take a plate out in the morning when the room is cool.
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MLPismyOPSEC
That One Ponyfucker


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Re: Cultivation General Discussion [Re: Inthepit] 1
#27102246 - 12/22/20 09:37 AM (3 years, 1 month ago) |
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Quote:
GrinchGrower said: I don't think the act of opening the PC at 0 PSI versus completely cooled makes much of a difference in how much air hits the bottles (because lids are wrapped with tin foil as well), it is a pretty slim chance of contam at that stage.
Actually there is a small but important difference. When you crack the PC at 0 PSI, the everything inside (steam, jars, grain, liquid, whatever) is still at a high enough temp to kill contams so as you tighten the lid, any contams on your hand get kilt dead. Granted, you could also make the argument that tightening the lid after natural cooldown is just fine as well due to indirect pathing which stops a lot of contams. Honestly either way you go is a-okay.
Quote:
GypsyBastard said: The air inside the jar and outside the jar are going to cool at pretty much the same rate and are both sterile. Contam'd air can only come from outside the PC. When the lid is opened after cooling there is no pressure differential between jar and PC and air will not instantly be driven from outside to inside pc, through obstructed pathways and into the jar in the time it take to tighten it. Think of the air under the foil as the air in a SAB.
EDIT: to clarify. The lid also serves this purpose and the foil can be seen as superfluous even if not using actual media bottles. Your entire PC acts like a sort of SAB once cooled and when I was using half pint or pint jars are media bottles, the pressure differential when cooling sorta seals the lid back down even when left loose. So any contams that make it into the PC will fall mostly straight down and would have no way to go back up and into the bottle/jar.
QFT!
Quote:
junk_f00d said: im still trying to narrow down my contam issue. last open plate test passed but I've noticed almost ALL of my plates get horribly moldy after a few months if stored at room temp on some random shelf, is that normal? i parafilm wrap them, but I'm starting to think my technique while wrapping may contribute to this issue (I cut my parafilm in a totally non-sterile manner, but wrap it up carefully in front of the filter face).
All of them contaming is very concerning! I do have the same issue where plates that sit empty for 6 weeks+ develop contams, but it's at a rate of ~30%. Plates that were poured at the same time but inoculated do not contam at all which i find weird. I wrap the empty plates (using Glad cling wrap) basically as soon as the agar cools, and i get normal/light amounts of condensation. When you wrap, do you try to keep the plate as horizontally level as you can? That's the big thing i pay attention to, and my contam rate of inoculated plates is <1% but blank plates ~30%. (Another side note, this data was all from my SAB days. Got a hood a month ago, have some new empty plates sitting so we will see if the hood changed anything)
Edited by MLPismyOPSEC (12/22/20 09:38 AM)
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GypsyBastard
Misanthrope


Registered: 11/30/20
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Re: Cultivation General Discussion [Re: Inthepit]
#27102284 - 12/22/20 10:17 AM (3 years, 1 month ago) |
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Quote:
Inthepit said:
Hi GypsyBastard, can you give a reference for that? I can't seem to find a/g here or in DuckDuckGo...thanks
A/G? Its in here somewhere but no I did not save the convo for future reference. 

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starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
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Re: Cultivation General Discussion [Re: GypsyBastard]
#27102353 - 12/22/20 11:04 AM (3 years, 1 month ago) |
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Anyone got some rough ballpark math on what a hydrated quart of coir would weigh dry? Trying to calculate some BE here using napkin math.
Just doing my own ballparking here in that lets say I hydrated a ~650g brick to 8 quarts then add two quarts of verm and each quart of verm is 110g.. So that's what 760g/10 giving me something like 76g dry substrate in a full quart.
Then I usually use 450g of dry WBS per jar. So each of my muda-esque bottles uses ballpark 57 grams of substrate and around 150 grams of dry WBS.
So if BE is total fresh weight of mushrooms over dry materials and dry materials is let's make it simple at 200g then 300-310 wet grams is only 150% BE?
-------------------- Listen, I'm steel fisted with the iron lung Heavy metal ballads out the guitar where lions run.
 
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GypsyBastard
Misanthrope


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Re: Cultivation General Discussion [Re: starbones]
#27102360 - 12/22/20 11:07 AM (3 years, 1 month ago) |
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I may be misunderstanding but wouldn't 1qt of dry coir be 650g/8=81.25g?
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