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GypsyBastard
Misanthrope


Registered: 11/30/20
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Re: Cultivation General Discussion [Re: GrinchGrower]
#27100897 - 12/21/20 01:10 PM (3 years, 1 month ago) |
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Nein. There is a tapered, or rather chamfered I think is the correct term, lip inside that I thought was just to make a good seal. It is angled so that when pressure builds up inside the bottle it can push its way out but not back in. I can't say for sure that they do not have a life span or number of uses before they should no longer be considered sound but I have not seen any issues with repeated use.
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clockworkshroom
Stranger



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Re: Cultivation General Discussion [Re: GrinchGrower]
#27100916 - 12/21/20 01:25 PM (3 years, 1 month ago) |
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Ok, I'm not going to do ms to grain, I'll be patient with the agar. I guess I just have first time anxiety that I won't get anything on my plates.
I inoculated 8 plates on 12th Dec, 2 dead to fluffy hairy pin shit, 5 no sign of anything at all, 1 some maybe mycelium growth, transferred to 4 plates on 20th
20 plates on 19th, mixture of drops from syringe and inoculation loop from a sterilised shot glass. Zero sign of anything yet.
20 plates last night. No sign of anything yet.
I'm a bit unsure about the inoculation loop Tek. My loops are fucking tiny and the wire is really thin, I've been scraping Zs in them but I'm not sure I'm picking up enough solution when I dip.
Here they all are lol (actually I have 6 in another cupboard). The syringes came from 2 very well regarded UK sources, temp is 71c.
If I get 1 healthy plate out of all of them I'll be happy 
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starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 18 days
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If you ever get a chance just get your hands on prints.
I have waaaaay more success on agar using a print than a spore syringe anyday of the week. I just toss a fresh needle on a syringe barrel because they cost all of five cents then using the sharp edge I scrap spore onto the agar then drag it through the agar in my streaking pattern. Hasn't failed me yet and for as little as I end up using the spore prints I've collected will last me until I'm probably dead in the ground.
-------------------- Listen, I'm steel fisted with the iron lung Heavy metal ballads out the guitar where lions run.
 
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GypsyBastard
Misanthrope


Registered: 11/30/20
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Re: Cultivation General Discussion [Re: starbones]
#27100939 - 12/21/20 01:37 PM (3 years, 1 month ago) |
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If you have some MSS left and just want to get something going, throw some PF jars together. You'll be harvesting them when your doing a2g so you'll get to fruition soon but without compromising anything and better chance of success than MSS to grain.
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clockworkshroom
Stranger



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Re: Cultivation General Discussion [Re: GypsyBastard]
#27100953 - 12/21/20 01:44 PM (3 years, 1 month ago) |
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I must confess I also bought a grow kit out of pure impatience, likely a big waste I know, I'll do some pf jars too. I think I can get a print in the UK, I'll try, when you use the needle do you flame it first? Cool it on the plate?
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starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 18 days
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I am starting a new experiment this week using a ten foot measuring tape, a monotub and a fuckin papaya.
Stay tuned.
-------------------- Listen, I'm steel fisted with the iron lung Heavy metal ballads out the guitar where lions run.
 
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GypsyBastard
Misanthrope


Registered: 11/30/20
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Quote:
clockworkshroom said: I must confess I also bought a grow kit out of pure impatience, likely a big waste I know, I'll do some pf jars too. I think I can get a print in the UK, I'll try, when you use the needle do you flame it first? Cool it on the plate?
Correct in flaming the needle. When using a scalpel I tap the blade in the agar to cool it before making cut but with a needle, cooling the tip isn't going to help the spores as they pass through the upper part of the needle. Just squirt a bit through the needle. It will cool pretty quickly. I usually squirt it in the bottom of my SAB but doing so into the plate wouldn't be an issue I think other than excess moisture.
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clockworkshroom
Stranger



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Re: Cultivation General Discussion [Re: GypsyBastard]
#27100981 - 12/21/20 02:06 PM (3 years, 1 month ago) |
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Just ordered verm, brf, perlite, 6 x 8oz mason jars. Guess I'm doing pf tek whilst I wait on my agar to do a monotub 
To be honest I want to experiment with everything so I can one day be the one giving advice.
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Schemenhaft
Oachkatzlschwoaf



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Re: Cultivation General Discussion [Re: GrinchGrower]
#27100997 - 12/21/20 02:18 PM (3 years, 1 month ago) |
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My cake started pinning over the last days. Like around a corner with 20% of the cake looks dry to me, also the myc started to bruise means it gets blueish.
Think its because of the bad air-flow, tried another kind of box with a bit of experimenting in my modifications. What should I do to rehydrate these spots? Humidity is fine inside.
Just wait till the end of the first flush and then give it a good 24h soak or should I dunk it right now? Not sure, read about it in a few older threads, but not sure this is maybe an old level of knowledge.
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GypsyBastard
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Registered: 11/30/20
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I completely understand and salute that mentality. I would even go so far as to say go ahead and try the MSS to grain if you have the supplies and time and desire. There are a number of reasons people discourage it and, unfortunately, some of those reasons are solely that they were once told not to. It is not a good method but I have tried it and did gain at least some experience and knowledge from doing so. Even if it is just from the practice and familiarity with sterile tek and procedure, that is still knowledge gained.
I don't want to come off as thinking I have all the answers or am an expert as I certainly am not. Nor do I want to be seen as giving contradictory advice, either to myself or the community because there are a lot of brilliant people here. But if you know what you are walking into, then yeah go for it. Give it a shot and see what happens. As long as you are not counting on it for success and you gain something from it, then it's a win. 
Sounds like you have the right attitude and I can't wait to see your results when they start coming in.  

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GrinchGrower
N00B



Registered: 10/02/20
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Re: Cultivation General Discussion [Re: GypsyBastard]
#27101018 - 12/21/20 02:28 PM (3 years, 1 month ago) |
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If you have the MSS available just have fun with it! In the worst case scenario, you will learn what not to do...
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RhYzo
D1g1t@l Dw3ll3r



Registered: 01/30/19 
Posts: 287
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Re: Cultivation General Discussion [Re: GypsyBastard]
#27101037 - 12/21/20 02:42 PM (3 years, 1 month ago) |
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@gypsybastard
I was told last night that liners are a one time use type of thang in another thread and so I chucked it up due to maybe the plastic becoming erroded or warped to the point where it's unusable again. Didn't ask why. (Maybe it was just their method only allowed it to be used once? I dunno. No biggie!)
I'll give her a try in the future since you've had success with it. Thanks
--------------------
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clockworkshroom
Stranger



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Re: Cultivation General Discussion [Re: RhYzo]
#27101108 - 12/21/20 03:29 PM (3 years, 1 month ago) |
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Thanks Gypsy bastard, I plan to celebrate my first fruit almost as passionately as I did the birth of my daughter haha.
I want to learn and will do all I need to, I'm lucky enough to have the resources to experiment a bit and try different things and that's a blessing. I've read so many teks over the last year but I'm one of those people that learns far better when I'm actually doing it so might mess around with things that are generally not recommended if it means more practice.
For now I'm not going ms to grain though, I'll save my oats for some good agar, I'll pf tek the remaining solution I have and see what happens!
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GypsyBastard
Misanthrope


Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
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Quote:
Schemenhaft said: My cake started pinning over the last days. Like around a corner with 20% of the cake looks dry to me, also the myc started to bruise means it gets blueish.
Think its because of the bad air-flow, tried another kind of box with a bit of experimenting in my modifications. What should I do to rehydrate these spots? Humidity is fine inside.
Just wait till the end of the first flush and then give it a good 24h soak or should I dunk it right now? Not sure, read about it in a few older threads, but not sure this is maybe an old level of knowledge.
I would either give them a heavy mist or try injecting some water into them. I don't know for sure, but I think a 24 dunk at this stage would do more harm than good. How close to harvest? are we talking a couple days or a week? If you are very close, just wait til harvest then dunk and try again.
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junk_f00d


Registered: 12/04/15
Posts: 933
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: GypsyBastard]
#27101735 - 12/21/20 09:12 PM (3 years, 1 month ago) |
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im still trying to narrow down my contam issue. last open plate test passed but I've noticed almost ALL of my plates get horribly moldy after a few months if stored at room temp on some random shelf, is that normal? i parafilm wrap them, but I'm starting to think my technique while wrapping may contribute to this issue (I cut my parafilm in a totally non-sterile manner, but wrap it up carefully in front of the filter face).
i think my grain is just consistently prepped a little too wet, so I've been trying to get it more and more dry. haven't done agar work in too long (been camping on some (tested clean) LC's and master jars). im getting to the point where I'm about to buy a replacement hepa filter just to satisfy my troubleshooting conerns.
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junk_f00d


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Re: Cultivation General Discussion [Re: GypsyBastard]
#27101737 - 12/21/20 09:13 PM (3 years, 1 month ago) |
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Quote:
GypsyBastard said:
Quote:
junk_f00d said: so what's the deal with tightening the lid on an agar media bottle as soon at the PC hits 0psi? I would think that would invite tons of dirty air to be sucked into the media bottle (though it'd still be so hot at this point it'd likely kill most invaders..?)
i don't get why you'd ever want to do that over lettign it cool down naturally. i guess with a natural cool down, you're still drawing in dirty air, just at a slower rate, so perhaps it's better to do it all at once while it's still hot enough to kill shit? is that the idea?
I always let them cool naturally, was never in a rush to open it hot and didn't know that was a thing honestly. However, I used to put piece of foil over the lid with the idea that contam'd air will not freely flow through obstructed pathways and give you the time needed to open pc, reach in, and tighten lids fully once cooled without fear of introducing 'tams. I also used to throw some tape over the lid pin as soon as it dropped and keep the weight on the steam valve to again reduce any chance of 'tam'd air making it to the media bottles.
HOWEVER, This was all done before learning that true media bottles with GL45 lids are designed to vent themselves during sterilization so that is NO NEED to have them cracked in the first place. Just tighten them down, they vent at temp/pressure, then seal as the cool. Easy peasy
thanks for the info, i didn't know that about GL45s either. i've never really understood the use of foil when you don't have a filter, however. hot air is still gonna want to escape, and thus draw in cool/dirty air.. right?
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GypsyBastard
Misanthrope


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Re: Cultivation General Discussion [Re: junk_f00d]
#27101755 - 12/21/20 09:27 PM (3 years, 1 month ago) |
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Quote:
junk_f00d said:
Quote:
GypsyBastard said:
Quote:
junk_f00d said: so what's the deal with tightening the lid on an agar media bottle as soon at the PC hits 0psi? I would think that would invite tons of dirty air to be sucked into the media bottle (though it'd still be so hot at this point it'd likely kill most invaders..?)
i don't get why you'd ever want to do that over lettign it cool down naturally. i guess with a natural cool down, you're still drawing in dirty air, just at a slower rate, so perhaps it's better to do it all at once while it's still hot enough to kill shit? is that the idea?
I always let them cool naturally, was never in a rush to open it hot and didn't know that was a thing honestly. However, I used to put piece of foil over the lid with the idea that contam'd air will not freely flow through obstructed pathways and give you the time needed to open pc, reach in, and tighten lids fully once cooled without fear of introducing 'tams. I also used to throw some tape over the lid pin as soon as it dropped and keep the weight on the steam valve to again reduce any chance of 'tam'd air making it to the media bottles.
HOWEVER, This was all done before learning that true media bottles with GL45 lids are designed to vent themselves during sterilization so that is NO NEED to have them cracked in the first place. Just tighten them down, they vent at temp/pressure, then seal as the cool. Easy peasy
thanks for the info, i didn't know that about GL45s either. i've never really understood the use of foil when you don't have a filter, however. hot air is still gonna want to escape, and thus draw in cool/dirty air.. right?
The air inside the jar and outside the jar are going to cool at pretty much the same rate and are both sterile. Contam'd air can only come from outside the PC. When the lid is opened after cooling there is no pressure differential between jar and PC and air will not instantly be driven from outside to inside pc, through obstructed pathways and into the jar in the time it take to tighten it. Think of the air under the foil as the air in a SAB.
EDIT: to clarify. The lid also serves this purpose and the foil can be seen as superfluous even if not using actual media bottles. Your entire PC acts like a sort of SAB once cooled and when I was using half pint or pint jars are media bottles, the pressure differential when cooling sorta seals the lid back down even when left loose. So any contams that make it into the PC will fall mostly straight down and would have no way to go back up and into the bottle/jar.
Edited by GypsyBastard (12/21/20 10:13 PM)
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GypsyBastard
Misanthrope


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Re: Cultivation General Discussion [Re: RhYzo]
#27101769 - 12/21/20 09:45 PM (3 years, 1 month ago) |
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Quote:
Sigh4.fg said: @gypsybastard
I was told last night that liners are a one time use type of thang in another thread and so I chucked it up due to maybe the plastic becoming erroded or warped to the point where it's unusable again. Didn't ask why. (Maybe it was just their method only allowed it to be used once? I dunno. No biggie!)
I'll give her a try in the future since you've had success with it. Thanks
Just hand a thought..... mushroom bags used in place of jars are a 1 time use thing. Could this be what they were talking about? For liners you can use a trash bag, grocery bag, painters plastic, just about anything of that nature.
No worries either way, I just suddenly had the thought that maybe that was the source of some confusion.
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junk_f00d


Registered: 12/04/15
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Re: Cultivation General Discussion [Re: GypsyBastard]
#27101862 - 12/21/20 11:38 PM (3 years, 1 month ago) |
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the whole 'superfluous' aspect of foil is what strikes me as odd. I get it when you have polyfill or some other filter you don't want soaked. but I'm having big contam issues currently so if foil would help, even annecdotally, I don't care how superfluous it is. I only use foil if I have a filter or some other port I don't want to get too wet. sometimes I'll use foil because it's nice having the 'sab' effect on my lids before innoculating masters, in theory at least, but that's really it.
doesn't your arguement kind of then support opening the PC while hot (i.e, there IS a pressure differential) being bad? I've just never understood that but see it done a lot lately, RR always said that was a no-no (not that he's the law or anything but his words went under careful scrunity at least), but like in Bod's tek he goes so far as to instruct the tek follower to immediately tigthen the GL45 lid once the PSI hits zero.
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GypsyBastard
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Re: Cultivation General Discussion [Re: junk_f00d]
#27101874 - 12/22/20 12:02 AM (3 years, 1 month ago) |
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Quote:
junk_f00d said: so what's the deal with tightening the lid on an agar media bottle as soon at the PC hits 0psi?
Quote:
GypsyBastard said:
I always let them cool naturally, was never in a rush to open it hot and didn't know that was a thing honestly.
Not sure where we went wrong here?
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