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RhYzo
D1g1t@l Dw3ll3r



Registered: 01/30/19
Posts: 287
Loc: Wormhole
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Re: Cultivation General Discussion [Re: starbones]
#27100190 - 12/21/20 12:54 AM (3 years, 1 month ago) |
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Quote:
starbones said: Tamp down the sides of your substrate with your fingers decently packed. Add more substrate to fill in the trench you made. Will slow the evaporation from the airy sides that's been causing them to knot and pin there.
Let the surface be the point of the greatest evaporation.
Lol I never thought about filling the trench with more sub haha. I'll try that next run. Thanks!
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A.k.a
Stranger



Registered: 10/27/19
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Re: Cultivation General Discussion [Re: RhYzo] 2
#27100236 - 12/21/20 02:47 AM (3 years, 1 month ago) |
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Yeah I do that too, cram the sides down and then re level the tub and pack them in again and then repeat until it stays level. That’s also the way to get the most out of a liner if you use one, packing the sides keeps it flush with the liner and it really gets pulled in by the myc.
I use tap water for everything and never had problems with sub or casing.
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LAGM2020     
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Schemenhaft
Oachkatzlschwoaf



Registered: 01/14/17
Posts: 339
Loc: Right side of the big poo...
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Re: Cultivation General Discussion [Re: A.k.a]
#27100273 - 12/21/20 04:15 AM (3 years, 1 month ago) |
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What do u guys think about this jar?
After almost one week after inoculation it looks like this, everywhere in the jar are spots of mycelium now. I just inoculated one piece of agar - But I had to shake the glass around like crazy because the agar was sticking to the upper wall of the jar.
Is this maybe the reason why everywhere myc starts to grow? Or is it maybe mold or something ? If its just cube-mycelium, is there any disadvantage of shaking the jars crazy after agar2grain for faster colonization?
Edited by Schemenhaft (12/21/20 04:17 AM)
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Mtbromo
The Bro


Registered: 08/27/20
Posts: 186
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Re: Cultivation General Discussion [Re: A.k.a] 1
#27100275 - 12/21/20 04:18 AM (3 years, 1 month ago) |
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Quote:
A.k.a said: Yeah I do that too, cram the sides down and then re level the tub and pack them in again and then repeat until it stays level. That’s also the way to get the most out of a liner if you use one, packing the sides keeps it flush with the liner and it really gets pulled in by the myc.
I use tap water for everything and never had problems with sub or casing.
I did tap down the sides but by the sound of it was to gentle.
Seriously have some 3D sub art going on RR recommended putting it on a wire rack was such a good idea just harder keeping it moist outside the tib, built a sub tent out of wire and plastic wrap lol to try and keep the humidity up #ghettoboytricks
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clockworkshroom
Stranger



Registered: 02/20/20
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Re: Cultivation General Discussion [Re: Mtbromo]
#27100406 - 12/21/20 07:04 AM (3 years, 1 month ago) |
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Sorry guys I try not to ask questions in here that are covered in zillions of threads but after literally reading for a year I still want to clarify on lids. I have 2 piece metal lids on my jars.
When I do a2g I will use bods polyfil eaf Tek but whilst I impatiently wait to get my agar perfect and get as close to isolating as I can I'm going to do ms to grain as I have a spare syringe and want to experiment.
Should I use different lids if I want to inoculate the jars from syringe? Or should I just use bods Tek and open them quick in a sab? Or even inject through the polyfil?
What's the most fool proof lid tek for ms to grain?
Thanks as always
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joze



Registered: 11/10/20
Posts: 928
Loc: PNW
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Re: Cultivation General Discussion [Re: Schemenhaft] 1
#27100424 - 12/21/20 07:19 AM (3 years, 1 month ago) |
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Quote:
Schemenhaft said: What do u guys think about this jar?
After almost one week after inoculation it looks like this, everywhere in the jar are spots of mycelium now. I just inoculated one piece of agar - But I had to shake the glass around like crazy because the agar was sticking to the upper wall of the jar.
Is this maybe the reason why everywhere myc starts to grow? Or is it maybe mold or something ? If its just cube-mycelium, is there any disadvantage of shaking the jars crazy after agar2grain for faster colonization?

It looks like mycelium to me, I don't think it's mold. The more you shake the jar, the more the agar will break up and distribute throughout the jar. I think that some people use softer agar recipes for inoculating grains so that they disintegrate when they shake.
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starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 18 days
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MS to grain is a bad idea.. however if you're hellbent on doing it use as little grain as possible per jar and try to outrun contaminations. The more grain to colonize the more likely the contaminations will win this race.
You don't need anything more than a lid with a filter if you're using a sab or flowhood. Wipe the jars down with iso, remove the ring, whipe the threads with iso then crack the lid a touch and squirt onto the grains. Prepare to failure here so spread your seed widely. When I used to do it I'd put around 250ml of grain per jar and do 12 jars with 1ml (Most MS syringes are 12ml, 2ml past the 10ml mark)
A few jars would of course be junk but the best looking jars went to shoeboxes where I tried to 1:1 spawn/sub because again, trying to outrun contamination as best as possible.
This is a bad idea but I figure you're going to do it anyway.
-------------------- Listen, I'm steel fisted with the iron lung Heavy metal ballads out the guitar where lions run.
 
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Schemenhaft
Oachkatzlschwoaf



Registered: 01/14/17
Posts: 339
Loc: Right side of the big poo...
Last seen: 2 years, 8 months
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Quote:
clockworkshroom said: Sorry guys I try not to ask questions in here that are covered in zillions of threads but after literally reading for a year I still want to clarify on lids. I have 2 piece metal lids on my jars.
When I do a2g I will use bods polyfil eaf Tek but whilst I impatiently wait to get my agar perfect and get as close to isolating as I can I'm going to do ms to grain as I have a spare syringe and want to experiment.
Should I use different lids if I want to inoculate the jars from syringe? Or should I just use bods Tek and open them quick in a sab? Or even inject through the polyfil?
What's the most fool proof lid tek for ms to grain?
Thanks as always
It doesn't matter which kind of lid/modified lid u use and how u inoculate. Its easy, when you inoculate with a sporesyringe which is not 100% sterile (and they are almost never) you'll also inoculate besides your wanted spores also unwanted spores/bacteria/..
In this case its not necessary to talk about the less-contaminating way of inoculating, if u inoculate contaminated stuff.
Just decide on your own whats easier for you - I decided to stop modify my lids in future, got the best results this time with just opening them - I mean, its not that complicated after a bit of time.
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GypsyBastard
Misanthrope


Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
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Re: Cultivation General Discussion [Re: RhYzo]
#27100641 - 12/21/20 10:19 AM (3 years, 1 month ago) |
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Quote:
Sigh4.fg said:
But I wanted to do liners before I found out that I couldn't reuse them 
Wait? you can't reuse liners?! I total have been. Whats bad about reusing liners? They arent sterile when you start anyway so give em a rinse and throw it back in there.
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The Dalcassian
Dirty Druggie



Registered: 01/04/17
Posts: 952
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Re: Cultivation General Discussion [Re: Schemenhaft]
#27100646 - 12/21/20 10:25 AM (3 years, 1 month ago) |
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I've always figure "If something ain't broke don't fix it" but I've seen a fair few posts of late talking about PC-ing grains for 2hrs. Is this the norm?
I've always done 10quarts for 90 minutes in my presto. I get the odd jar going funny, last one was an APE jar in September, but I've done 50 odd jars since then, without issue. What does everyone else do?
-------------------- Here Lies This Individual 
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GypsyBastard
Misanthrope


Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
Last seen: 2 years, 10 months
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Re: Cultivation General Discussion [Re: Schemenhaft]
#27100648 - 12/21/20 10:27 AM (3 years, 1 month ago) |
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Quote:
Schemenhaft said:
Quote:
clockworkshroom said: Sorry guys I try not to ask questions in here that are covered in zillions of threads but after literally reading for a year I still want to clarify on lids. I have 2 piece metal lids on my jars.
When I do a2g I will use bods polyfil eaf Tek but whilst I impatiently wait to get my agar perfect and get as close to isolating as I can I'm going to do ms to grain as I have a spare syringe and want to experiment.
Should I use different lids if I want to inoculate the jars from syringe? Or should I just use bods Tek and open them quick in a sab? Or even inject through the polyfil?
What's the most fool proof lid tek for ms to grain?
Thanks as always
It doesn't matter which kind of lid/modified lid u use and how u inoculate. Its easy, when you inoculate with a sporesyringe which is not 100% sterile (and they are almost never) you'll also inoculate besides your wanted spores also unwanted spores/bacteria/..
In this case its not necessary to talk about the less-contaminating way of inoculating, if u inoculate contaminated stuff.
Just decide on your own whats easier for you - I decided to stop modify my lids in future, got the best results this time with just opening them - I mean, its not that complicated after a bit of time.
 Work with what you have and what is easiest for you at this point. This stuff can be fun and if you just looking for a reason to make some super cool SHIP lids and SFDs and shit, go for it! But if you just want to get some myc growing, then yeah just open the jar in an SAB, squirty-squirt, close it up and let magic happen. I will say, beyond the obligatory "spores to grain is bad" statement, it seems to help if the grains are cracked open just slightly. Not really "burst" but overdone just enough that the shells crack and the spores have a place to sorta take hold.
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GypsyBastard
Misanthrope


Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
Last seen: 2 years, 10 months
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Quote:
The Dalcassian said: I've always figure "If something ain't broke don't fix it" but I've seen a fair few posts of late talking about PC-ing grains for 2hrs. Is this the norm?
I've always done 10quarts for 90 minutes in my presto. I get the odd jar going funny, last one was an APE jar in September, but I've done 50 odd jars since then, without issue. What does everyone else do?
I saw those too and kinda caught my eye. Mostly because I had completely forgot that I had read similar before until seeing it. Right before I got out of cult a while back. I had always only done/read 90mins and was having success but the occasional jar going fucky. It was while I was playing around with bags that someone told me 120min was the norm for grain and even longer for bags. I stopped cult not long after and never really tried it then forgot when I restarted. I can't say for sure one way or the other but I can say that I had a lot of success with only ever doing 90min cycles but it was not 100%.
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The Dalcassian
Dirty Druggie



Registered: 01/04/17
Posts: 952
Loc: Emerald lsles
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Re: Cultivation General Discussion [Re: GypsyBastard]
#27100665 - 12/21/20 10:40 AM (3 years, 1 month ago) |
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Ya just that, I'd read 90mins when I started and that was that. I couldn't confidently say I've had issue with my grains, I've always assumed any issues were down to hitch hikers on my agar. It makes sense to pc for longer times depending on how loaded the pc is, I'd just never thought of it, and never thought to look into it until now
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junk_f00d


Registered: 12/04/15
Posts: 933
Last seen: 1 year, 2 months
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so what's the deal with tightening the lid on an agar media bottle as soon at the PC hits 0psi? I would think that would invite tons of dirty air to be sucked into the media bottle (though it'd still be so hot at this point it'd likely kill most invaders..?)
i don't get why you'd ever want to do that over lettign it cool down naturally. i guess with a natural cool down, you're still drawing in dirty air, just at a slower rate, so perhaps it's better to do it all at once while it's still hot enough to kill shit? is that the idea?
Edited by junk_f00d (12/21/20 12:21 PM)
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Fietchen
Stranger
Registered: 08/27/19
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Re: Cultivation General Discussion [Re: junk_f00d]
#27100820 - 12/21/20 12:25 PM (3 years, 1 month ago) |
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Cherrs
How much time it's needed to see the first mycelium Growing in my Liquid Culture? I am in worry if the spores are in to it because i couldn't see any spores in my Liquid Culture maybe they are to small for the eye? I hope the spores are OK.
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GrinchGrower
N00B



Registered: 10/02/20
Posts: 682
Last seen: 2 years, 8 months
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Quote:
The Dalcassian said: I've always figure "If something ain't broke don't fix it" but I've seen a fair few posts of late talking about PC-ing grains for 2hrs. Is this the norm?
I've always done 10quarts for 90 minutes in my presto. I get the odd jar going funny, last one was an APE jar in September, but I've done 50 odd jars since then, without issue. What does everyone else do?
I follow Spitball's Quick Rye Prep and do them for 1.75hr @ 15+ PSI Only on my second run of grain so I can't attest to the efficacy, but not seeing any weird contams (yet) in the first batch after inoculation w/ agar.
Quote:
junk_f00d said: so what's the deal with tightening the lid on an agar media bottle as soon at the PC hits 0psi? I would think that would invite tons of dirty air to be sucked into the media bottle (though it'd still be so hot at this point it'd likely kill most invaders..?)
i don't get why you'd ever want to do that over lettign it cool down naturally. i guess with a natural cool down, you're still drawing in dirty air, just at a slower rate, so perhaps it's better to do it all at once while it's still hot enough to kill shit? is that the idea?
Are you thinking the act of tightening it down draws in air?
You tighten it down because it was left slightly cracked (like 1/16th turn) to allow gas to escape during the PC cycle.
Otherwise you could explode the media bottle or create tight vapor locks.
I don't think the act of opening the PC at 0 PSI versus completely cooled makes much of a difference in how much air hits the bottles (because lids are wrapped with tin foil as well), it is a pretty slim chance of contam at that stage.
Edited by GrinchGrower (12/21/20 12:32 PM)
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The Dalcassian
Dirty Druggie



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Re: Cultivation General Discussion [Re: GrinchGrower]
#27100833 - 12/21/20 12:30 PM (3 years, 1 month ago) |
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I think the odds are you won't either. It's probably just prudent to to run the pc for longer and if you run into issues with your grain then you know that's one thing you can adjust straight away
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joze



Registered: 11/10/20
Posts: 928
Loc: PNW
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Re: Cultivation General Discussion [Re: GrinchGrower]
#27100842 - 12/21/20 12:38 PM (3 years, 1 month ago) |
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Quote:
GrinchGrower said:
Quote:
The Dalcassian said: I've always figure "If something ain't broke don't fix it" but I've seen a fair few posts of late talking about PC-ing grains for 2hrs. Is this the norm?
I've always done 10quarts for 90 minutes in my presto. I get the odd jar going funny, last one was an APE jar in September, but I've done 50 odd jars since then, without issue. What does everyone else do?
I follow Spitball's Quick Rye Prep and do them for 1.75hr @ 15+ PSI Only on my second run of grain so I can't attest to the efficacy, but not seeing any weird contams (yet) in the first batch after inoculation w/ agar.
I also used Spitball's Quick Rye Prep. I've mostly seen people recommending 90 minutes for grains at 15 psi, but 2 hours for grains at 12 psi (Instapot).
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GypsyBastard
Misanthrope


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Re: Cultivation General Discussion [Re: junk_f00d] 1
#27100849 - 12/21/20 12:41 PM (3 years, 1 month ago) |
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Quote:
junk_f00d said: so what's the deal with tightening the lid on an agar media bottle as soon at the PC hits 0psi? I would think that would invite tons of dirty air to be sucked into the media bottle (though it'd still be so hot at this point it'd likely kill most invaders..?)
i don't get why you'd ever want to do that over lettign it cool down naturally. i guess with a natural cool down, you're still drawing in dirty air, just at a slower rate, so perhaps it's better to do it all at once while it's still hot enough to kill shit? is that the idea?
I always let them cool naturally, was never in a rush to open it hot and didn't know that was a thing honestly. However, I used to put piece of foil over the lid with the idea that contam'd air will not freely flow through obstructed pathways and give you the time needed to open pc, reach in, and tighten lids fully once cooled without fear of introducing 'tams. I also used to throw some tape over the lid pin as soon as it dropped and keep the weight on the steam valve to again reduce any chance of 'tam'd air making it to the media bottles.
HOWEVER, This was all done before learning that true media bottles with GL45 lids are designed to vent themselves during sterilization so that is NO NEED to have them cracked in the first place. Just tighten them down, they vent at temp/pressure, then seal as the cool. Easy peasy
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GrinchGrower
N00B



Registered: 10/02/20
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Re: Cultivation General Discussion [Re: GypsyBastard]
#27100867 - 12/21/20 12:48 PM (3 years, 1 month ago) |
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Oh, this is (good) news to me! Do you see any deformation of the GL45 seal/lid doing that repeatedly?
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