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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: Cultivation General Discussion [Re: Josex]
#27099294 - 12/20/20 12:52 PM (3 years, 1 month ago) |
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starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 18 days
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Re: Cultivation General Discussion [Re: Mycolorado]
#27099350 - 12/20/20 01:32 PM (3 years, 1 month ago) |
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With the consistency of how my muda-esque bottles have been performing I'm documenting a new journal coming up here doing a side-by-side-by-side-by-side run using the same culture.
Run 1) WBS without canola seed. +CVG Run 2) WBS custom mix with a decent amount of canola seed (For the oils) +CVG Run 3) WBS without canola seed +CVG supplemented with coconut oil. Run 4) WBS custom mix with canola +CVG supplemented with coconut oil.
Supplementation with virgin coconut oil was done by making an oil emulsion that was added to coir at a rate of 20ml/~650g coir brick.
What am I hoping to find here? Fuck knows really.
-------------------- Listen, I'm steel fisted with the iron lung Heavy metal ballads out the guitar where lions run.
 
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: starbones]
#27099378 - 12/20/20 01:53 PM (3 years, 1 month ago) |
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 Look how convenient lol Talking about the leeway you have before grains turn excessively dark by PC'ing too long
I just took this pic of a jar I sterilized yesterday. I accidentally ran the PC for 3 hours and 20 minutes at 15 psi and the grains still look good. Barley can take a lot of punishment, though.
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clockworkshroom
Stranger



Registered: 02/20/20
Posts: 655
Last seen: 2 years, 7 months
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Re: Cultivation General Discussion [Re: Josex]
#27099610 - 12/20/20 04:19 PM (3 years, 1 month ago) |
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I've gone a bit agar crazy and inoculated about 50 plates in the last week (my first ever agar attempts). I suppose it's good practice even if a bit wasteful.
I've used several different GT syringes, a couple have been in a fridge for months, a couple of new ones.
Little sign of anything yet other than the one I posted a pic of on the previous page. I transferred 4 segments of that to new dishes earlier.
Of the 50 (haha) other dishes, if/when any look good I'll do the same and keep transferring til I have good uniformity.
Once I have dishes that are ready to go to grain I had planned to do a monotub using bods unmodified Tek but as the fruit quality will be a complete unknown to me is there a more efficient way to do it? Should I try quicker smaller cakes to figure if they'll be any good?
I've read that a perfect looking plate could still produce bad fruit and I'm thinking if I go down the monotub route then in a couple of months time I might have literally nothing to show for it if I'm working with bad genetics.
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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That's a lot of plates indeed lol. Good thing is you can select the fuck out of those and only keep the best of the best few and toss the rest of the lot, because it isn't practical to keep so many cultures of a given variety unless you're into some kind of weird proyect.
You shouldn't worry about non-fruiting strains, at any rate if they exist at all they're extremely rare to the point I doubt you'll ever encounter them in your life if you did 50 monotubs every month until the day you die. Just focus on getting good clean growth on agar, follow a proven grain prep, get some coir and grow ez shroomz.
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clockworkshroom
Stranger



Registered: 02/20/20
Posts: 655
Last seen: 2 years, 7 months
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Re: Cultivation General Discussion [Re: Josex]
#27099682 - 12/20/20 05:06 PM (3 years, 1 month ago) |
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Thanks man, in that case then yeah from this 50 or so I don't plan to be keeping any once I've successfully transferred and moved on to the next stage. It's just been good practice and in these days of lockdown a hobby like this is awesome.
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Well actually you can keep them all and wait to see if those plates pin invitro. Clones from invitro agar pins tend to not disappoint.
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clockworkshroom
Stranger



Registered: 02/20/20
Posts: 655
Last seen: 2 years, 7 months
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Re: Cultivation General Discussion [Re: Josex]
#27099720 - 12/20/20 05:32 PM (3 years, 1 month ago) |
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Ok cool, I may as well in that case. Do I literally just take the whole pin and drop it on a new dish?
Also what's the common method for disposing of lots of dishes if it is needed? Normal rubbish is probably fine I guess.
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Quote:
clockworkshroom said:
Do I literally just take the whole pin and drop it on a new dish?
Yup
Quote:
clockworkshroom said:
Also what's the common method for disposing of lots of dishes if it is needed? Normal rubbish is probably fine I guess.
Yea I don't think many people will be interested in opening your trash bags.
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LtLurker
Lost Sailor



Registered: 01/03/18
Posts: 7,535
Loc: Borderlands
Last seen: 6 days, 3 hours
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Re: Cultivation General Discussion [Re: Josex]
#27099730 - 12/20/20 05:44 PM (3 years, 1 month ago) |
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It's probably over kill and paranoid, but i keep plates in their own trash bags. Then if it's somehow discovered, atleast it's not sitting with an envelope from my house. I also don't have trash pickup so i kinda feel like public dumpsters are more likely to have an accidently discovery somehow.
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Yeetusdeetus



Registered: 11/23/19
Posts: 1,242
Last seen: 4 hours, 50 minutes
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Re: Cultivation General Discussion [Re: LtLurker]
#27099818 - 12/20/20 06:57 PM (3 years, 1 month ago) |
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Not if you go to those trash centers with the compacting containers
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GypsyBastard
Misanthrope


Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
Last seen: 2 years, 10 months
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Re: Cultivation General Discussion [Re: Yeetusdeetus]
#27099832 - 12/20/20 07:03 PM (3 years, 1 month ago) |
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There. Settled once and for all.....
Finished my Doing "Science" part 2 test on grain hydration. Looks like about 7floz:2.5cups H2O:WBS. I'll try PC cycle hydration hopefully tonight or tomorrow and see if the observations hold up in practice.
Anyway, what are you guys working on? any fun projects??
 
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BondVillain
Stranger



Registered: 08/14/18
Posts: 118
Last seen: 2 years, 10 months
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Re: Cultivation General Discussion [Re: Yeetusdeetus]
#27099837 - 12/20/20 07:06 PM (3 years, 1 month ago) |
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Update:
I inoculated the 5 1/2 pint jars on Thursday using the BRF PF tek. I'm storing the jars in a glass door cabinet. It's gets a little ambient light and the temp is around 68-70 degrees. Is this sufficient to colonize mycelium? Should I use an incubator box? Also, how long should it be before I notice any growth?
Thanks
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GypsyBastard
Misanthrope


Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
Last seen: 2 years, 10 months
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Re: Cultivation General Discussion [Re: BondVillain]
#27099843 - 12/20/20 07:11 PM (3 years, 1 month ago) |
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Quote:
BondVillain said: Update:
I inoculated the 5 1/2 pint jars on Thursday using the BRF PF tek. I'm storing the jars in a glass door cabinet. It's gets a little ambient light and the temp is around 68-70 degrees. Is this sufficient to colonize mycelium? Should I use an incubator box? Also, how long should it be before I notice any growth?
Thanks
Conditions sound great. no need for incubator. 5 days or so. If you don't see anything, just wait longer.
You're on your way. Congrats!! 

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Phony Phone

Registered: 09/19/20
Posts: 612
Last seen: 6 days, 16 hours
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Re: Cultivation General Discussion [Re: GypsyBastard]
#27099948 - 12/20/20 08:32 PM (3 years, 1 month ago) |
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Safe to mist 50/50 peat verm casing pH'd @ 7.5~ with tap water or am i looking at possible contam issues?
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 10 hours
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Re: Cultivation General Discussion [Re: Phony Phone]
#27099962 - 12/20/20 08:44 PM (3 years, 1 month ago) |
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Quote:
Phony Phone said: Safe to mist 50/50 peat verm casing pH'd @ 7.5~ with tap water or am i looking at possible contam issues?
Tap water won't kill your casing as long as it was properly pasteurized.
My question is can an improperly ph balanced casing cause side pins? (Not that yours is, I'm concerned about mine)
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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The Dalcassian
Dirty Druggie



Registered: 01/04/17
Posts: 952
Loc: Emerald lsles
Last seen: 9 months, 4 days
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Re: Cultivation General Discussion [Re: tryptkaloids]
#27100113 - 12/20/20 11:08 PM (3 years, 1 month ago) |
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I was reading on the woodlovers thread that if the sub is properly colonised you can just add boiling water to your casing layer and let sit (like bucket tek) for a a few hours, then your good to go. This was recommended for a sterilised sawdust sub but I would think it would be the same for any fully colonised sub
-------------------- Here Lies This Individual 
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Mtbromo
The Bro


Registered: 08/27/20
Posts: 186
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I keep getting side pins in the unmodified tubs, Im keeping the surface well misted, What else would cause this?
Would painting the sides or using a liner stop it?
Its super fucking annoying, had to take the whole sub out and put it on a wire rack while its 360 degree pinning! looks sorta cool but very akward and annoying to manage
-------------------- Knowledge and Healing in a Handful.
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starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 18 days
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Re: Cultivation General Discussion [Re: Mtbromo] 3
#27100171 - 12/21/20 12:13 AM (3 years, 1 month ago) |
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Tamp down the sides of your substrate with your fingers decently packed. Add more substrate to fill in the trench you made. Will slow the evaporation from the airy sides that's been causing them to knot and pin there.
Let the surface be the point of the greatest evaporation.
-------------------- Listen, I'm steel fisted with the iron lung Heavy metal ballads out the guitar where lions run.
 
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RhYzo
D1g1t@l Dw3ll3r



Registered: 01/30/19
Posts: 287
Loc: Wormhole
Last seen: 3 months, 11 days
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Re: Cultivation General Discussion [Re: Mtbromo] 1
#27100182 - 12/21/20 12:34 AM (3 years, 1 month ago) |
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Quote:
Mtbromo said: I keep getting side pins in the unmodified tubs, Im keeping the surface well misted, What else would cause this?
Would painting the sides or using a liner stop it?
Its super fucking annoying, had to take the whole sub out and put it on a wire rack while its 360 degree pinning! looks sorta cool but very akward and annoying to manage
I wanted to try liners because they help quite a bit and the removal of the sub is way easier. I just can't stand wasting plastic.
I don't have but a few side pins which grow straight out usually to where I can pick them easily, or cut. No bother to me at all. What I do is tamp the sides down all along the tub as tight as I can. I figured that the mycelium will try to grow towards the path of least resistance?
But I wanted to do liners before I found out that I couldn't reuse them 
Maybe also concentrate most of the misting away from the sides and keep it in the middle? Just a thought. Good luck!
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