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clockworkshroom
Stranger



Registered: 02/20/20
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Re: Cultivation General Discussion [Re: GrinchGrower]
#27098107 - 12/19/20 05:04 PM (3 years, 1 month ago) |
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Just poured 20 plates from agar sterilised in it. Going in to inoculate in 30 mins or so, I'm sure it will have no impact but will report if it does.
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clockworkshroom
Stranger



Registered: 02/20/20
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How long should I wait to inoculate a stack of 20 poured plates? Is 45 mins too soon?
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Phony Phone

Registered: 09/19/20
Posts: 612
Last seen: 6 days, 15 hours
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The mono im working on now refuses to evaporate those little water beads where they also are larger towards its center.
I loosened the polyfill layers and used a fan. Its still stubborn. 6 days in now and no pinning. Suggestions?
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sh4d0ws
LSx


Registered: 02/26/08
Posts: 12,086
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Re: Cultivation General Discussion [Re: Phony Phone]
#27098158 - 12/19/20 05:42 PM (3 years, 1 month ago) |
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6 days from spawn??
Pins can take 3 weeks to form for some varieties. Even longer for PE. My quickest clones don't start pinning until day 11 (TOC).
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Phony Phone

Registered: 09/19/20
Posts: 612
Last seen: 6 days, 15 hours
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Re: Cultivation General Discussion [Re: sh4d0ws]
#27098183 - 12/19/20 05:55 PM (3 years, 1 month ago) |
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Oh boy. That coupled with the possibility of some trich lurking..
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GypsyBastard
Misanthrope


Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
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Re: Cultivation General Discussion [Re: Phony Phone]
#27098240 - 12/19/20 06:32 PM (3 years, 1 month ago) |
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Odd thought, and just but... Any chance the inside could have been exposed to acetone?
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GrinchGrower
N00B



Registered: 10/02/20
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Re: Cultivation General Discussion [Re: Phony Phone]
#27098409 - 12/19/20 08:40 PM (3 years, 1 month ago) |
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Quote:
Phony Phone said: 6 days in now and no pinning. Suggestions?
Leave them alone for 2 weeks
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GypsyBastard
Misanthrope


Registered: 11/30/20
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Re: Cultivation General Discussion [Re: GrinchGrower]
#27098458 - 12/19/20 09:32 PM (3 years, 1 month ago) |
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Doin' a thing....again....
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GypsyBastard
Misanthrope


Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
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Quote:
clockworkshroom said: How long should I wait to inoculate a stack of 20 poured plates? Is 45 mins too soon?
It's really just about temp. As long as agar is solid and cooled to room temp, you're good to go. Condensation can be an issue so I usually let the sit overnight to let them cool and the temp differential to equalize a bit. All of that is said from the point of view of an SAB. If using a flowhood I would imagine much less time needed to cool but principle remains the same. heat kills, so just make sure they are cool them hit the ground running. 
I have a feeling this reply comes late but figured late info is better than no info. 
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Schemenhaft
Oachkatzlschwoaf



Registered: 01/14/17
Posts: 339
Loc: Right side of the big poo...
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Re: Cultivation General Discussion [Re: Failboat]
#27098567 - 12/19/20 11:40 PM (3 years, 1 month ago) |
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Quote:
Quirkmeister92 said: Well if it's for fun then do you have any more aesthetic vessels? There's a whole thread for novelty grows in fun containers.
Thank you for the hint with the fun container thread! First time I have heard about it, seems to be a nice thread with awesome ideas and results!
I have more containers with maybe nice results, but most of them will need a bit more colonized grain as I can currently use for just fun-projects. But there is another idea with a more interesting container - Wouldn’t call ist aesthetic but the result is interesting if it works like I hope.
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GypsyBastard
Misanthrope


Registered: 11/30/20
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Re: Cultivation General Discussion [Re: Schemenhaft]
#27098584 - 12/20/20 12:07 AM (3 years, 1 month ago) |
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Before I drift off to sleepy land, I wanted to share some insight from tonights works in Doing "Science" part 2. Night and  
Interesting side notes and conclusions
--------600ml of WBS was scooped from the top of an unopened 20lb bag into a 1000ml beaker-----------500ml WBS was poured from the 1000ml beaker into a 500ml beaker and weighed 379.1g/ gives 75.82g/100ml --Remaing 100ml WBS was added to the 500ml beaker and weighed 476.5g/6 gives 79.41g/100ml Measures and weights were done in this way for reason; to try to find some meaningful average of weight to volume of WBS.At first, the numbers looked pretty good. The 500ml weigh gave a good sample size for kernel variation. The 100ml weigh a sort of random selection small sample. Left with this, WBS would be given an average Mass/Volume of 77.615g/100ml. And I would have been WRONGThere is nothing wrong with those numbers. No math magician so computers were used to minimize errors. But there is a hidden data point....-- 600ml=476.5g -500ml=379.1g _________________ 100ml=97.4g!!!! That last 100ml in the beaker was in itself an excellent sample. About 20g more than the other 100ml sample averages.
Bringing us to our conclusion thus far: WBS has an average Mass/Volume of (75.82+79.41+97.4)\3 84.21g/100ml
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shroomdav
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Registered: 12/20/20
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Re: Cultivation General Discussion [Re: GypsyBastard]
#27098658 - 12/20/20 01:33 AM (3 years, 1 month ago) |
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Hi guys, first time grower here. Can you please take a quick look at my pic.. whats that black stuff? It was there suddenly today - spores?
Also is that blue color a problem? I think it's bruising.. i had to break the glasses as the cakes were stuck. And i just read today that i shouldn't mist them directly lol
Edited by shroomdav (12/20/20 01:35 AM)
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RhYzo
D1g1t@l Dw3ll3r



Registered: 01/30/19
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Loc: Wormhole
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Re: Cultivation General Discussion [Re: shroomdav]
#27098666 - 12/20/20 02:00 AM (3 years, 1 month ago) |
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Black/purple stuff is definitely spores.
Blue is bruising as far as I know.
I like to clap a few cakes but you beat them up pretty hard Daddy. Direct misting probably caused that as well.
Chill Daddy, chill. Looks good as far as I know. Not too familiar with the Tek your using but you got fruits so now you can only improve from here.
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Edited by RhYzo (12/20/20 02:05 AM)
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Schemenhaft
Oachkatzlschwoaf



Registered: 01/14/17
Posts: 339
Loc: Right side of the big poo...
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Re: Cultivation General Discussion [Re: shroomdav]
#27098667 - 12/20/20 02:03 AM (3 years, 1 month ago) |
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Quote:
shroomdav said: Hi guys, first time grower here. Can you please take a quick look at my pic.. whats that black stuff? It was there suddenly today - spores?
Also is that blue color a problem? I think it's bruising.. i had to break the glasses as the cakes were stuck. And i just read today that i shouldn't mist them directly lol

The black spot could be spores from older mushrooms u have harvested maybe? Note 100% sure with just this picture, but if u have harvested shrooms over the black spot (or maybe there are shrooms over the black spot currently(not sure because of the angle..)? - You see the clear rounded line and the "half circle" which is still white in the black spot? Seems like there was something in its way while spores felt down, what explains this half-circle.
So if there were Shrooms/are shrooms over this area, u should be fine. Otherwise I don't know what it is either.
Lets go to ur second question - You have crazy kind of bruising on your mycelium and also on your shrooms. Bruising is usually not a big deal but in your case it is the result of a very very thirsty mushroom-organism. Seems like the cakes are dry as hell - Mycelium starts bruising more and more the more thirsty it gets. You should stop misting directly and you need to rehydrate your cakes. Never did your way of growing but I would recommend to put your cakes on a dish with water over night so it can rehydrate.
Keep an eye on it, maybe just over night is not enough and it needs more water.
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RhYzo
D1g1t@l Dw3ll3r



Registered: 01/30/19
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Re: Cultivation General Discussion [Re: Schemenhaft]
#27098670 - 12/20/20 02:13 AM (3 years, 1 month ago) |
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Also use the search function, it's your best friend.
Use key words that might cover what answers you may be seeking.
"Blue brusing on cakes" "Rehydrating cakes" Etc etc
Search function tip: change the 'posted on' date to get you the just recent topics and conversations that may be the most up to date methods that include said key words. Not always true to what you may be looking for butit's another way to utilize the search function better.
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shroomdav
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Re: Cultivation General Discussion [Re: RhYzo]
#27098676 - 12/20/20 02:20 AM (3 years, 1 month ago) |
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Thanks for your help guys!
Yeah bruising is pretty terrible probably because I had do break the glasses.. I hydrated them 24hours and spray them 4 times/day with 2 times/day fanning. I will rehydrate them today.
I did not harvest before.. but yesterday I had to move the chamber so I guess that's where the spores fell off from the bigger ones on the bottom of the picture.
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clockworkshroom
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Re: Cultivation General Discussion [Re: shroomdav]
#27098912 - 12/20/20 08:13 AM (3 years, 1 month ago) |
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This dish is now 8 days old, it's from my first ever attempt at agar, my sab Tek was pretty poor and it was really supposed to be an attempt to practice pouring but I inoculated anyway from a syringe. I used 2 different syringes, 4 dishes each. A few have clear fluffy contamination, a few nothing has grown on at all but this one I'm not sure of.
It's completely flat on the dish, no upwards growth and about 3/4 inch diameter. It doesn't look as white as I expect mycelium to and it's taken 8 days to get to this size which seems slow at around 69f. Anyone know what it is?
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GypsyBastard
Misanthrope


Registered: 11/30/20
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Can't say for sure but I see no reason for concern. Myc can look a bit off when it first germinates. Even if it looks 100% clean, being a germ plate you would want to do at least 1 transfer anyway. Now is a good size to go ahead and do your first transfer IMO.
As for the other plates, that's the beauty of agar. As long as they're not green yet, I would do the same and take some transfers to try to clean them up. Hope that helps 
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clockworkshroom
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Re: Cultivation General Discussion [Re: GypsyBastard]
#27098930 - 12/20/20 08:27 AM (3 years, 1 month ago) |
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Thanks, I have my new unused scalpel ready to go so will put it to the test. How big a section should I transfer? Rice grain?
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Quote:
clockworkshroom said: Thanks, I have my new unused scalpel ready to go so will put it to the test. How big a section should I transfer? Rice grain?
As small as you can manage. If you think you can go smaller, go smaller.
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