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OfflineBrownBear
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Registered: 06/05/20
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Re: Cultivation General Discussion [Re: Culliganman360]
    #27096061 - 12/18/20 03:46 PM (3 years, 1 month ago)

Sh4dows, I make sure the lid is as level as I can make it and tighten it down before turning on the burner. There is absolutely no leaking from the lid while its running. No. I have never used vaseline or any other lubricant. Tbh, I never had a problem until I tried to stuff 6 bags into it.

Cullingman360, the AA is fine. The only damage was the plastic stuck to the walls. I was able to get about 90% of it off. I'm sure the rest of it will wear off over time.


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Invisiblemushhead
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Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
Re: Cultivation General Discussion [Re: BrownBear]
    #27096200 - 12/18/20 05:02 PM (3 years, 1 month ago)

I had to change out my presto PC pressure gage because of this issue.
It wasn't reading right, telling me 13psi, but by all means it was knocking at 15psi obviously.
Nothing wrong with my PC at all other than the gage being fucked up. :shrug:


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OfflineGrinchGrower
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Registered: 10/02/20
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Re: Cultivation General Discussion [Re: mushhead]
    #27096354 - 12/18/20 06:19 PM (3 years, 1 month ago)

I have been working with agar since I started with five or six different varieties, in addition to staggering some (relatively) successful MS grows using PF tek, BRF to bulk, and MS to rye shoeboxes that are all in various stages currently.

My agar plates (started from MSS) are a lot more uncertain however... getting into the T-4/5 range they are looking better (and I had one nice T-3 I was able to spawn with).

My question is kind of subjective, but is it worth the effort of continuing to pursue these original agar isolating projects which are getting closer to "clean", or should I just switch entirely to cloning samples that are known to fruit?
I am finding the one or two clones I have taken show more vigorous rhizo growth on the plates anyway and will likely require less transfers.

Maybe I'm just putting the cart before the horse... :confused:


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OfflinecronicrFacebook
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Re: Cultivation General Discussion [Re: GrinchGrower]
    #27096368 - 12/18/20 06:25 PM (3 years, 1 month ago)

clones will often show the vigorous growth simply du to the switch from tertiary mycelium back to vegetative.
if you have clean plates with limited genetics I would run them and pick clones.
ya never know when your missing winners just looking at plates


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineInthepit
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Registered: 08/20/19
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Re: Cultivation General Discussion [Re: cronicr]
    #27096440 - 12/18/20 07:07 PM (3 years, 1 month ago)

Boy howdy, just looking at plates. It's a real learning experience.
And some white stuff looks like right stuff.
And then one transfers and transfers...
And on and on...
I could really use a success, I'm thinking go back to RW or PESA..
They gave me πŸ’•...


--------------------
:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:


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Offlinebw86
Doesn't play well with others


Registered: 11/12/06
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Re: Cultivation General Discussion [Re: Inthepit]
    #27096454 - 12/18/20 07:15 PM (3 years, 1 month ago)

Hey cron.
Is there anything I should do on agar to help dikaryons co-exists? Look for a certain pattern ?

I am trying to do the exact opposite of everyone else and combine a diversity of genetics into one spawn culture.Multi Isolate


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OfflineLtLurker
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Re: Cultivation General Discussion [Re: bw86]
    #27096480 - 12/18/20 07:25 PM (3 years, 1 month ago)

Dikariotic myc will cross and share genetic material, anostasmosis i think it's called. Someone fill me in on the right term if you know it please.

Or maybe i misunderstand your question cause you say your not trying to cross, but the post reads like you wanna mix up the genetics and see what you get. You're gonna end up with something like a pundant(sp?) square with different combinations of dominant and recessive traits. You can than work to stabilize the traits(or see more recessive traits expressed) in subsequent generations.


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Offlinebw86
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Re: Cultivation General Discussion [Re: LtLurker]
    #27096521 - 12/18/20 07:40 PM (3 years, 1 month ago)

I want to stabilize co-existing Clones into one master jar,I want to see multiple isolates growing side by side.


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OfflineLtLurker
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Re: Cultivation General Discussion [Re: bw86]
    #27096584 - 12/18/20 08:06 PM (3 years, 1 month ago)

Oh i see. I don't think there's really a way to accomplish that. 2 varieties in the same tub you could definitely do by just spawning different grains on either side of a tub and don't mix them together. But coexisting in a single grain jar and handle being mix up doesn't sound plausible. you might get a few of each pheno, but many would mix genes.

Or maybe i'm about to learn something new here to. :shrug:


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OfflinecronicrFacebook
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Re: Cultivation General Discussion [Re: bw86] * 1
    #27096598 - 12/18/20 08:17 PM (3 years, 1 month ago)

Quote:

bw86 said:
I want to stabilize co-existing Clones into one master jar,I want to see multiple isolates growing side by side.



If you can get a couple clonesvthat form a wall on agar without throwing off a third sector they will work in a jar


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinebw86
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Re: Cultivation General Discussion [Re: LtLurker] * 1
    #27096600 - 12/18/20 08:17 PM (3 years, 1 month ago)

Yeah i really don't know.Everyone seems to be hesitant to answer. the closest i found is this

-----
Quote:

fahtster said:
if you take the LC of a clone and mix it with the LC of another clone of a different variety, do both those clones have to be monokaryotic for a cross to happen?

Faht





Quote:

cronicr said:
You can't get monokaryons from a clone, once monokaryons fuse to become dikaryons those 2 monokaryons cease to exist.
Yes dikaryons can fuse but not always and more rarely.
So if you took to clones and mixed them in one lc I'd give it about a 10% chance at best, if you were to put isolates together on agar you would see where I pull that number from.



-------

Edit- cron thanks for a response, obviously you responded as i was typing. I will leave my above post for other people to read.


Edited by bw86 (12/18/20 08:20 PM)


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InvisibleCulliganman360
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Registered: 06/05/19
Posts: 854
Re: Cultivation General Discussion [Re: bw86]
    #27096613 - 12/18/20 08:26 PM (3 years, 1 month ago)

I recently bought a few tubs and I dident notice they have a small opening where the lid meets the handles ... think I could still run them unmoded?
Or should I try covering these holes with saran wrap


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OfflineLtLurker
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Re: Cultivation General Discussion [Re: cronicr]
    #27096627 - 12/18/20 08:34 PM (3 years, 1 month ago)

yea thanks cron. I didn't know they could do that and missed the earlier comment as well.


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InvisiblePastywhyteMDiscord
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Re: Cultivation General Discussion [Re: LtLurker] * 1
    #27096642 - 12/18/20 08:44 PM (3 years, 1 month ago)

Although it’s been shown that you can dedikaryotize the myc via blending in saline solutions. I know it’s been done with Pleurotus before but I think Sidnee Oversinglinton also did it with cubes.


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OfflineEugene Gesuale
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Re: Cultivation General Discussion [Re: Pastywhyte]
    #27096670 - 12/18/20 09:02 PM (3 years, 1 month ago)

:camping:


--------------------
Everything in life is a trade-off.

All posts made by this account are purely satirical in nature.


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InvisibleTedsDead
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Re: Cultivation General Discussion [Re: Eugene Gesuale]
    #27096983 - 12/19/20 02:13 AM (3 years, 1 month ago)

obersingleton


--------------------
weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396


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OfflinePhony Phone

Registered: 09/19/20
Posts: 612
Last seen: 6 days, 14 hours
Re: Cultivation General Discussion [Re: TedsDead]
    #27097230 - 12/19/20 08:00 AM (3 years, 1 month ago)

How long is it usually from first appearance of trich to its sporulation? 24, 48 hrs?


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Offlinejcm4620
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Re: Cultivation General Discussion [Re: Phony Phone]
    #27097234 - 12/19/20 08:01 AM (3 years, 1 month ago)

well put it this way once u see it. its too late


--------------------
PANAEOLUS FRUITING MADE SIMPLE



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OfflinePhony Phone

Registered: 09/19/20
Posts: 612
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Re: Cultivation General Discussion [Re: jcm4620]
    #27097240 - 12/19/20 08:05 AM (3 years, 1 month ago)

spotted a blobby matty surface in my tub that is white, waiting to see if it turns any green at all so i can give it a proper funeral.
I first saw that stuff  like 12 hrs ago.


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InvisibleWall.E
Bacteria's Bitch
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Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
Re: Cultivation General Discussion [Re: Phony Phone]
    #27097270 - 12/19/20 08:21 AM (3 years, 1 month ago)

Did it spread significantly?


--------------------
Life’s shit, but I’m loving it



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