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Invisiblesh4d0ws
LSx
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Registered: 02/26/08
Posts: 12,086
Re: Cultivation General Discussion [Re: mushboy]
    #27088969 - 12/14/20 02:38 PM (3 years, 1 month ago)

Got a 23 qt presto gasket at home hardware for $15 CAD

Cheaper than ordering from presto because no shipping cost :thumbup:, I actually think it is right around the price presto charges for just the gasket when converting USD to CAD

Canadians if your presto gasket goes, check your local home hardware :super:


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Offlinestarbones
I'm an alien, I eat uranium.
I'm a teapot


Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 18 days
Re: Cultivation General Discussion [Re: sh4d0ws]
    #27088971 - 12/14/20 02:40 PM (3 years, 1 month ago)

Home Hardware sells the kit with the blow-out plug yeah. Just check the box when you go there though. The kit I bought some asshole put their used gasket in and returned.


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Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.




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Offlinejunk_f00d
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Registered: 12/04/15
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Re: Cultivation General Discussion [Re: starbones]
    #27089008 - 12/14/20 03:03 PM (3 years, 1 month ago)

I had 5 PE tubs sit for about 2 weeks at 100% colonization (fruiting conditions introduced at spawning), no signs of pinning and now I'm seeing little specs of trich so I'm gonna have to toss the tubs/move them outside.

I forgot to case, but other than that anyone have  diagnosis here? Surface conditions looked pretty good the whole time. Perhaps a little too wet (I never misted because of that). I guess the only thing I can blame it on the excess moisture maybe leading to bacteria which in turn gave trich a nice platform. Sound about right? On a related note, if my sub is too wet, with too much water pooling, probably best to crack the lid and turn on a fan right? Or maybe laying down a sheet of paper towels to soak up some water?


Edited by junk_f00d (12/14/20 03:07 PM)


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InvisibleMtbromo
The Bro


Registered: 08/27/20
Posts: 186
Re: Cultivation General Discussion [Re: junk_f00d]
    #27089017 - 12/14/20 03:06 PM (3 years, 1 month ago)

What would make your fruits spawn and go rotten while they are at Vail break stage?

So weird! To much misting? Hheellpp???


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Knowledge and Healing in a Handful.


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Offlinejunk_f00d
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Re: Cultivation General Discussion [Re: Mtbromo]
    #27089021 - 12/14/20 03:08 PM (3 years, 1 month ago)

Quote:

Mtbromo said:
What would make your fruits spawn and go rotten while they are at Vail break stage?

So weird! To much misting? Hheellpp???



Contams don't really care what stage you're at :^) They'll turn mature fruits to mush. OR you could be mistaking spore drop for rot. If your caps are black it may just be spores.


Edited by junk_f00d (12/14/20 03:09 PM)


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Offlinestarbones
I'm an alien, I eat uranium.
I'm a teapot


Registered: 03/04/20
Posts: 1,131
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Re: Cultivation General Discussion [Re: Mtbromo]
    #27089027 - 12/14/20 03:10 PM (3 years, 1 month ago)

Damp conditions can lead to pseudomomas outbreaks, big bacterial rot. Misting further while you've got that going on can aerosolize the bacteria spreading it even worse. There's no big open caps anywhere dumping spore that's got you thinking it's rot when it's not?

Stipes turning into jelly near the base is trichoderma mold normally. When it's in the sub and feeding on your fruits you'll see them fall over because the trich is consuming the base.

Pictures would help but yeah, if it's the caps and such it's pseudomomas most likely.


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Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.




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OfflineShroomhunts
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Re: Cultivation General Discussion [Re: LtLurker]
    #27089030 - 12/14/20 03:11 PM (3 years, 1 month ago)

Quote:

LtLurker said:
Quote:

RenegadeMycologist said:
Can someone explain to me what is liquid culture.
And how do I make one.

I'm noob in cultivation.



It's agar without the agar hardener to grow out myc. Super easy once you get agar down.

My favorite take on this is an old post from Bod. Paraphrased: If you need directions For LC, than you aren't ready for LC.



LC was one of the first things I learned when I started. I was lucky enough to find a vendor that supplied lc syringes and made my own pretty easily, great way to have tons of quality inoculate if you are just getting started. Much cheaper than repeatedly buying spores or making your own syringes withless contamination risk. If you dont know agar try get your hands on a lc syringe they are a godsend to new cultivators.


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OfflineShroomhunts
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Re: Cultivation General Discussion [Re: junk_f00d]
    #27089041 - 12/14/20 03:17 PM (3 years, 1 month ago)

Quote:

junk_f00d said:
Quote:

Mtbromo said:
What would make your fruits spawn and go rotten while they are at Vail break stage?

So weird! To much misting? Hheellpp???



Contams don't really care what stage you're at :^) They'll turn mature fruits to mush. OR you could be mistaking spore drop for rot. If your caps are black it may just be spores.



If you are having mature Tubs contam you should check your temps and Humidity levels, I'd get that tub out asap and clean the whole area, once contam spores infest your work areas they can plague you for a long time to come.


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You never kno


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OfflineLtLurker
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Re: Cultivation General Discussion [Re: Shroomhunts]
    #27089060 - 12/14/20 03:25 PM (3 years, 1 month ago)

Quote:

Shroomhunts said:
Quote:

LtLurker said:
Quote:

RenegadeMycologist said:
Can someone explain to me what is liquid culture.
And how do I make one.

I'm noob in cultivation.



It's agar without the agar hardener to grow out myc. Super easy once you get agar down.

My favorite take on this is an old post from Bod. Paraphrased: If you need directions For LC, than you aren't ready for LC.



LC was one of the first things I learned when I started. I was lucky enough to find a vendor that supplied lc syringes and made my own pretty easily, great way to have tons of quality inoculate if you are just getting started. Much cheaper than repeatedly buying spores or making your own syringes withless contamination risk. If you dont know agar try get your hands on a lc syringe they are a godsend to new cultivators.



In many areas an lc syringe is illegal. Plus, you still don't know if they made their lc clean. That's relying on luck. Great you won the lottery on that, but too many newbs shoots spores into lc thinking it's gonna expand their spores and wind up with nothing when it's inevitably contaminated. Doing agar work is the only reliable way to inoc cleanly and test it before use.


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OfflineYeetusdeetus
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Re: Cultivation General Discussion [Re: LtLurker]
    #27089071 - 12/14/20 03:35 PM (3 years, 1 month ago)

Quote:

natedawgnow said:
I always hear bad things about celltreat but I've used them for years, many cases,
and never had any issues :shrug: never even had a broken plate

Try fablab or falcon



Quote:

mushboy said:
I've only used celltreat. Besides an obnoxiously oversized master carton they are always fine.




It’s not that they break, it’s just hard to pick the lid up without having my fingers under the lip. I have really poor dexterity

Maybe I’ll get some of these frog fingers so I don’t have to worry about skin flakes getting under the lid :lol:


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OfflineShroomhunts
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Re: Cultivation General Discussion [Re: LtLurker]
    #27089076 - 12/14/20 03:38 PM (3 years, 1 month ago)

Quote:

LtLurker said:
Quote:

Shroomhunts said:
Quote:

LtLurker said:
Quote:

RenegadeMycologist said:
Can someone explain to me what is liquid culture.
And how do I make one.

I'm noob in cultivation.



It's agar without the agar hardener to grow out myc. Super easy once you get agar down.

My favorite take on this is an old post from Bod. Paraphrased: If you need directions For LC, than you aren't ready for LC.



LC was one of the first things I learned when I started. I was lucky enough to find a vendor that supplied lc syringes and made my own pretty easily, great way to have tons of quality inoculate if you are just getting started. Much cheaper than repeatedly buying spores or making your own syringes withless contamination risk. If you dont know agar try get your hands on a lc syringe they are a godsend to new cultivators.



In many areas an lc syringe is illegal. Plus, you still don't know if they made their lc clean. That's relying on luck. Great you won the lottery on that, but too many newbs shoots spores into lc thinking it's gonna expand their spores and wind up with nothing when it's inevitably contaminated. Doing agar work is the only reliable way to inoc cleanly and test it before use.



Yeah when trying to get new cultures for edibles I always just buy LC syringes and shoot up a jar and bam practically infinite innoculant. It used to be very easy to get LC syringes for actives in the US despite the questionable legality. Not having to do agar work in less than optimal conditions can save you lots of time if you are a newb until you get the adequate supplies. If you can get your hands on them I'd definitely buy. Not worth the shitshow dealing with plates and sterile techniques until you want to go deeper into cloning and isolation.


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Offlineclockworkshroom
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Registered: 02/20/20
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Re: Cultivation General Discussion [Re: Yeetusdeetus]
    #27089077 - 12/14/20 03:38 PM (3 years, 1 month ago)

2 newb questions I can't see covered anywhere

1. For agar, can you use water boiled in a kettle rather than microwave it before the pc? I've seen people use boiling water but they always seem to do it in a pan. Are kettles bad?

2. When you pressure cook grain in a mason jar (polyfil lid tek) do you loosen the lid at all before wrapping in foil?

I asked in bod's threads but figured its better here and will delete from them


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OfflineShroomhunts
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Re: Cultivation General Discussion [Re: clockworkshroom]
    #27089081 - 12/14/20 03:42 PM (3 years, 1 month ago)

Quote:

clockworkshroom said:
2 newb questions I can't see covered anywhere

1. For agar, can you use water boiled in a kettle rather than microwave it before the pc? I've seen people use boiling water but they always seem to do it in a pan. Are kettles bad?

2. When you pressure cook grain in a mason jar (polyfil lid tek) do you loosen the lid at all before wrapping in foil?

I asked in bod's threads but figured its better here and will delete from them




1. Doesn't make a difference you are gonna PC it anyway, just use clean distilled water.
2. I tighten mine but also doesn't make a difference as long as they are snug. The gas exchange will be good through the polyfill and ball/Kerr lids are self sealing


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Offlinestarbones
I'm an alien, I eat uranium.
I'm a teapot


Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 18 days
Re: Cultivation General Discussion [Re: clockworkshroom]
    #27089088 - 12/14/20 03:45 PM (3 years, 1 month ago)

I use an induction kettle for everything. I prepare my agar in the kettle and pour it from there (I do no pours). I use tap water for everything these days except for spore syringes.

It's worth it to have one in the lab as it can get 1000ml of anything boiling hard fast.

No need for loose lids. Finger tight is good to go as you want the vacuum the jar will pull as it cools to pull air through the filter.


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Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.




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OfflineLtLurker
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Re: Cultivation General Discussion [Re: Shroomhunts]
    #27089092 - 12/14/20 03:48 PM (3 years, 1 month ago)

Quote:

Shroomhunts said:
Not having to do agar work in less than optimal conditions can save you lots of time if you are a newb until you get the adequate supplies. If you can get your hands on them I'd definitely buy. Not worth the shitshow dealing with plates and sterile techniques until you want to go deeper into cloning and isolation.



No. This is way off. Sure shooting spores straight to brf is quicker than weeks of plate work, but all it takes is a SAB to do plate work and there's nothing hard about it. Lotsa people start off with platework as newbs and do fine. You can stick a SAB in a moldy old shack and still make clean plates. The cleaner your innoculant the better your grow, cloning is just the natural progression and cherry on top.


Edited by LtLurker (12/14/20 03:52 PM)


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OfflineRoscoeReturnsS
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Re: Cultivation General Discussion [Re: clockworkshroom]
    #27089098 - 12/14/20 03:50 PM (3 years, 1 month ago)

Quote:

clockworkshroom said:
2 newb questions I can't see covered anywhere

1. For agar, can you use water boiled in a kettle rather than microwave it before the pc? I've seen people use boiling water but they always seem to do it in a pan. Are kettles bad?

2. When you pressure cook grain in a mason jar (polyfil lid tek) do you loosen the lid at all before wrapping in foil?

I asked in bod's threads but figured its better here and will delete from them




1. Boiled is boiled, doesn’t matter how you do it. I use a pan because I will add my malt extract and agar to the pan. This way I can boil prior to PC, and decant off the hot break. This is not a necessary step, so you could use a kettle if you wish.

2. When you PC grain, your jars need to be able to vent. If you have any type of filter, polyfill/tyvek/SFD, you don’t have to loosen the lid. If using unmodded lids, they need to be loosened to allow pressure to equalize. Only exception IME is the plastic lids. They tend to warp if tightened during the PC run. I loosen those even with a filter.


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InvisibleMtbromo
The Bro


Registered: 08/27/20
Posts: 186
Re: Cultivation General Discussion [Re: starbones]
    #27089112 - 12/14/20 03:57 PM (3 years, 1 month ago)

Quote:

starbones said:
Damp conditions can lead to pseudomomas outbreaks, big bacterial rot. Misting further while you've got that going on can aerosolize the bacteria spreading it even worse. There's no big open caps anywhere dumping spore that's got you thinking it's rot when it's not?

Stipes turning into jelly near the base is trichoderma mold normally. When it's in the sub and feeding on your fruits you'll see them fall over because the trich is consuming the base.

Pictures would help but yeah, if it's the caps and such it's pseudomomas most likely.




pseudomomas is mos def what's going on after researching it! Thanks a lot so annoying had a perfect canopy! Looks like I misted to much gonna leave the lid off all day try and dry it out


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OfflineShroomhunts
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Re: Cultivation General Discussion [Re: LtLurker]
    #27089121 - 12/14/20 04:08 PM (3 years, 1 month ago)

Quote:

LtLurker said:
Quote:

Shroomhunts said:
Not having to do agar work in less than optimal conditions can save you lots of time if you are a newb until you get the adequate supplies. If you can get your hands on them I'd definitely buy. Not worth the shitshow dealing with plates and sterile techniques until you want to go deeper into cloning and isolation.



No. This is way off. Sure shooting spores straight to brf is quicker than weeks of plate work, but all it takes is a SAB to do plate work and there's nothing hard about it. Lotsa people start off with platework as newbs and do fine. You can stick a SAB in a moldy old shack and still make clean plates. The cleaner your innoculant the better your grow, cloning is just the natural progression and cherry on top.



You are not wrong, just different ways to skin a cat, if you can get LC chances are its already a tried and proven clone that is going to colonize much faster than spores and Yeild better both in weight and potency than MS. Inoculating a LC jar with a LC syringe has got to be the fastest cheapest and easiest way to get a ton of innoculant so you can mass produce spawn.


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OfflineLtLurker
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Re: Cultivation General Discussion [Re: Shroomhunts]
    #27089151 - 12/14/20 04:27 PM (3 years, 1 month ago)

I'm mostly focusing on actives, not so much edibles as I don't have experience buying edible lc's from vendors.

But you bring up a huge IF from my perspective dude. If you're buying active LC's they can claim its anything. They can show pics of awesome fruits and say it's a high quality clone they worked, then actually ship you some bs they made putting spores into an LC or it could be an awesome clone but the vendor botched the batch. Like anything else pre-made(grain bags, kits, etc), you don't truly know the quality. Too many people do shady things for money. I have a hard time getting past the control you have making your own vs taking risks paying someone else.


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Offlinestarbones
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I'm a teapot


Registered: 03/04/20
Posts: 1,131
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Re: Cultivation General Discussion [Re: Shroomhunts]
    #27089161 - 12/14/20 04:33 PM (3 years, 1 month ago)

Some vendors or other sources make LC from MS cleaned up on agar. I do the same now for clone hunting purposes.

No guarantee you're getting an isolate when you receive an LC syringe and unless the culture was worked by someone reputable with proven results I'd want LC from MS all day long. Working genetics and finding something truly yours is absolutely rewarding but it takes organization, self-discipline, patience and knowledge.

Someone posted earlier in this thread that having a notebook is one of the best tools you can have in this hobby. Take notes, label things.. keep a record of what you cultivated and how it worked out. Keep masters of these things.


--------------------
Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.




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