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Offlinetryptkaloids
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I'm a teapot


Registered: 02/08/15
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Re: Cultivation General Discussion [Re: Sankhara]
    #27088693 - 12/14/20 11:43 AM (3 years, 1 month ago)

Whenever I question which route to take i do both


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineRenegadeMycologist
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #27088714 - 12/14/20 11:51 AM (3 years, 1 month ago)

Can someone explain to me what is liquid culture.
And how do I make one.

I'm noob in cultivation.


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:mushroom2:  l e a r n i n g  t h i n g s :mushroom2:


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OfflineSankhara
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Re: Cultivation General Discussion [Re: RenegadeMycologist] * 1
    #27088725 - 12/14/20 11:56 AM (3 years, 1 month ago)

Quote:

tryptkaloids said:
Whenever I question which route to take i do both




I was kind of expecting that answer! I guess I'll do both then, it wont hurt i guess.


Quote:

RenegadeMycologist said:
Can someone explain to me what is liquid culture.
And how do I make one.

I'm noob in cultivation.



Then I'd definitively recommend you stay away from LCs until you have a good grasp of agar work and sterile technique.

If you are still stubborn as fuck, then I'd recommend you go this route:
Josex' Poke: A different way of doing LC


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How would you rate the quality of my answer?


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OfflineLtLurker
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Re: Cultivation General Discussion [Re: RenegadeMycologist] * 1
    #27088738 - 12/14/20 12:03 PM (3 years, 1 month ago)

Quote:

RenegadeMycologist said:
Can someone explain to me what is liquid culture.
And how do I make one.

I'm noob in cultivation.



It's agar without the agar hardener to grow out myc. Super easy once you get agar down.

My favorite take on this is an old post from Bod. Paraphrased: If you need directions For LC, than you aren't ready for LC.


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OfflineRenegadeMycologist
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Re: Cultivation General Discussion [Re: LtLurker] * 1
    #27088747 - 12/14/20 12:06 PM (3 years, 1 month ago)

Of course I'm not ready for it, i am asking out of curiosity what a liquid culture is.

I thought it's like liquified(blended) agar with mycelium on it which is blended as well in the process...like slurry or something.

Ok sorry, I'm noob


Edited by RenegadeMycologist (12/14/20 12:09 PM)


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Offlinetedoro
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Re: Cultivation General Discussion [Re: RenegadeMycologist]
    #27088749 - 12/14/20 12:09 PM (3 years, 1 month ago)

Quote:

RenegadeMycologist said:
Can someone explain to me what is liquid culture.
And how do I make one.

I'm noob in cultivation.




LC (liquid culture) is mycelia growing in sweetened sterile water.

LI (Liquid Innoculant) Is mycelia grown on a material (usually agar) and blended up into sterile water.

Agar Drops (lots of names) is dropping mycelia grown on agar directly onto the grains.

Slurry is I guess technically an LI that uses PF cakes and blends them into sterile water.


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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OfflineLtLurker
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Re: Cultivation General Discussion [Re: RenegadeMycologist]
    #27088758 - 12/14/20 12:14 PM (3 years, 1 month ago)

Quote:

RenegadeMycologist said:
Of course I'm not ready for it, i am asking out of curiosity what a liquid culture is.

I thought it's like liquified(blended) agar with mycelium on it which is blended as well in the process...like slurry or something.

Ok sorry, I'm noob



Don't be sorry. I don't mean to come across like a smart-ass, it just reminded me of that post.


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InvisibleMoabfighter
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Registered: 12/13/15
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Re: Cultivation General Discussion [Re: tedoro]
    #27088761 - 12/14/20 12:16 PM (3 years, 1 month ago)

Dehydrating my first small batch in a few years. Good feeling man. Just pf tek but man it reignited my desire for the hobby. I have some WBS APE knotting in a shoebox now, and I have one jar I may try g2g with. Can’t wait to try this yellow millet and the red milo side by side. Found the yellow grains at a health food store for a dollar a pound so I grabbed 5lbs of it. Exciting ideas I’ve been thinking about


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KSSS And PE WBS.
 


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OfflineRenegadeMycologist
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Re: Cultivation General Discussion [Re: tedoro]
    #27088762 - 12/14/20 12:16 PM (3 years, 1 month ago)

.


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:mushroom2:  l e a r n i n g  t h i n g s :mushroom2:


Edited by RenegadeMycologist (08/16/23 05:51 AM)


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OfflineSchemenhaft
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Re: Cultivation General Discussion [Re: RenegadeMycologist]
    #27088842 - 12/14/20 01:09 PM (3 years, 1 month ago)

Made yesterday 8 jars with rye, tried different ways.
Some with polyfil-lid+aluminium foil covering, some just with polyfil-lid without foil, some just with an unmodified lied.

Did now Agar2grain from first generation Multispore-plates - They were still looking 100% clean after 10 days now.
Usually I go for minimum 2-3 transfers, but all of my plates (around 60) look 100% clean til now, just two of three with the growkit-mycelium transfer have a bacterial infection, but everything else would be admirable.

So I thought lets give it a try, nothing to loose and I get more and more practice and even self-research-experience.
My experience says "No way, spore-syringe from a basic-shop is never that clean to go agar2grain without a transfer before" - What do u guys think?
When this also works as good as my current work after the restart seems to be, then I'll need to find out why the hell I do such a good job in relation to 2017.


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Invisiblesh4d0ws
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Registered: 02/26/08
Posts: 12,086
Re: Cultivation General Discussion [Re: RenegadeMycologist]
    #27088856 - 12/14/20 01:19 PM (3 years, 1 month ago)

Quote:

RenegadeMycologist said:
Of course I'm not ready for it, i am asking out of curiosity what a liquid culture is.

I thought it's like liquified(blended) agar with mycelium on it which is blended as well in the process...like slurry or something.

Ok sorry, I'm noob





Blended agar or blended pf cakes or BRF pucks or whatever is called liquid inoculant, often referred to as LI

It is often called slurry as well but in case you ever see LI mentioned, that is another name for it.


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OfflineLtLurker
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Re: Cultivation General Discussion [Re: Schemenhaft]
    #27088865 - 12/14/20 01:26 PM (3 years, 1 month ago)

Quote:

Schemenhaft said:
Did now Agar2grain from first generation Multispore-plates - They were still looking 100% clean after 10 days now.
Usually I go for minimum 2-3 transfers, but all of my plates (around 60) look 100% clean til now, just two of three with the growkit-mycelium transfer have a bacterial infection, but everything else would be admirable.

So I thought lets give it a try, nothing to loose and I get more and more practice and even self-research-experience.
My experience says "No way, spore-syringe from a basic-shop is never that clean to go agar2grain without a transfer before" - What do u guys think?
When this also works as good as my current work after the restart seems to be, then I'll need to find out why the hell I do such a good job in relation to 2017.



I'd say any syringe. Unless you're using an invitro method to grow and make your syringe, then it's exposed to open air at some point. It's always a good idea to transfer and get away from any possible ride-alongs.

If you're already getting competent with plates, than LC isn't significantly harder. The "wait till your ready" comments are mainly to stop people from dumping their syringes into a LC. That josex link from earlier is a really cool method and since you got plates working, I really don't see the harm in attempting some lc to. Just don't commit everything to it and you aren't really losing anything if it doesn't work out just like you're saying.


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Offlinestarbones
I'm an alien, I eat uranium.
I'm a teapot


Registered: 03/04/20
Posts: 1,131
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Re: Cultivation General Discussion [Re: LtLurker]
    #27088877 - 12/14/20 01:35 PM (3 years, 1 month ago)

The caveat about LC is that unlike agar you don't have a clear picture of any hidden contamination.
There are visual cues that of course will let a person know about obvious contamination in them but beyond that ridealongs can be hard to spot. Ain't nothing like knocking up a hundred quarts of grain with a dirty culture.

I started with LC before agar though and had great success so take anything I say with a grain of salt.


--------------------
Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.




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OfflineLtLurker
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Re: Cultivation General Discussion [Re: starbones]
    #27088893 - 12/14/20 01:47 PM (3 years, 1 month ago)

True. but you can just put a drop of the lc to agar and know if it's clean.


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OfflineMLPismyOPSEC
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Re: Cultivation General Discussion [Re: pureshrooming]
    #27088904 - 12/14/20 01:57 PM (3 years, 1 month ago)

Quote:

tedoro said:
Try to study the bags when you pull them out, to see where they popped. My guess is that they popped as they filled the voids between the bags... the plastic got weak, expanded and popped. This is hastened by letting any steam bleed from the bobble/valve when the bags have reached full temp. I made a thread on the topic. (advice on first post) https://www.shroomery.org/forums/showflat.php/Number/26282176




I have looked at that, and reliably the bottom two form a shape that kinda looks like a neck pillow and they pop in the "corners" that form. I guess a better picture is to say the football shape that they form after the cycle, they pop at the points. I will definitely be checking out that thread!

Quote:

pureshrooming said:

I had the same damn problem and just a couple days ago saw someone post that the filter needs to be facing outside to allow steam escape. Immediately fixed my popped bag problem... the feeling of relief opening the PC and pulling out 6 perfect bags was almost like seeing my first ever pins in a tub lol

What layout are you doing to fit 7? I can fit 6 and have noticed there is a bit of spare room left but it doesn't seem like enough for another 4 lb bag




That's good news, that gives me hope for my next grain run! I laid two on flat on bottom (filter facing up), two on top of that same direction, and three on the top layer, turned 90* so the filter is facing horizontally. I might not do that next time, because you either have two filters facing each other, or two filters facing the bottom of another bag, which could cause the same issue. I don't remember if all three on top survived or not, but i believe they did. Placing the trivet on top, it was a tight fit, i had to cheat the trivet to one side so the pressure lock would allow me to even put the lid on in the first place!


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OfflineYeetusdeetus
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Re: Cultivation General Discussion [Re: MLPismyOPSEC]
    #27088944 - 12/14/20 02:22 PM (3 years, 1 month ago)

Can someone recommend me some decent, affordable petris? I hate these celltreats from amazon; the slight outward slope on the lid makes it difficult to grab without getting my fingers under it


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Invisiblenatedawgnow
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Re: Cultivation General Discussion [Re: Yeetusdeetus]
    #27088954 - 12/14/20 02:27 PM (3 years, 1 month ago)

I always hear bad things about celltreat but I've used them for years, many cases,
and never had any issues :shrug: never even had a broken plate

Try fablab or falcon


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OfflineSchemenhaft
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Re: Cultivation General Discussion [Re: LtLurker] * 1
    #27088955 - 12/14/20 02:27 PM (3 years, 1 month ago)

I'll go for LC later, did it in 2017 also with success.
Don't think I have problems with Agar, worked also okay three years ago, took a long break because of my space-situation.
Now have way more space for this hobby and wanna try everything.

But for LC I wanna grow out some boxes and get good genetics.

I'll see how my jars are doing the next couple of days - Maybe they will be fine, but I tried many stuf for the first time with these jars and the innculates, so there are many points why it could fail.
But these are just side-effects to get more experience and research-results on my own - Just because its fun.

Hope my growkit starts to pin the next days so I could get some good genetics from nice shrooms.
The restart from 0 is a bit tough because not that much to do, just doing something and then waiting days to weeks.
But when everything is runs well I have many things to do parallel - Got way to much time because of corona haha.


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Offlinestarbones
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Registered: 03/04/20
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Re: Cultivation General Discussion [Re: Yeetusdeetus]
    #27088956 - 12/14/20 02:28 PM (3 years, 1 month ago)

Gonna start adding a tablespoon or two of unhydrated WBS to my WBS jars before PCing, want there to be some good dry grain in there to succ up the excess carrier liquid from the LC pours. I don't see why it wouldn't work but gonna give it a whirl tonight. 10ml of LC per quart jar works but it's tricky with the water content.


--------------------
Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.




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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: natedawgnow]
    #27088957 - 12/14/20 02:28 PM (3 years, 1 month ago)

I've only used celltreat. Besides an obnoxiously oversized master carton they are always fine.


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