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tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,206
Last seen: 1 day, 1 hour
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Re: Cultivation General Discussion [Re: ModularMind] 1
#27084839 - 12/11/20 11:29 PM (3 years, 1 month ago) |
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I recently made some cement pucks, about the size of a hockey puck... to set atop my dishes. one for every dish. Haven't tried them yet. I made them when I realized the trick is to have the thing on the top cool slower than the agar plate itself. My oven warmed pucks achieve that.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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Egohst
Stranger

Registered: 08/09/20
Posts: 20
Loc: God’s Palm
Last seen: 2 years, 11 months
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Re: Cultivation General Discussion [Re: Yeetusdeetus]
#27084943 - 12/12/20 01:47 AM (3 years, 1 month ago) |
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Well I def know that when I squirted a whole PE6 syringe (BC my fridge broke) into my organic soil tub for my plants, they germinated in a week!!!! I have tons of bacteria in there from all kinds of compost plus tons of different added microbes. The soil is two years old and most def alive itself. The mycelium spread on the whole surface in a couple weeks then dried out to grey bc I’m not watering my giant tub of soil every day! Makes my think I could use it unpasteurized and I WILL be doing an experiment on one of my 30 grain quarts with my cannabis soil totally unpasteurized. This did not work with manure but I bet my spores wouldn’t have colonized just the manure totally untouched like they did the soil I made.
Hopefully I can just cycle my mushroom and cannabis soil. I def started dumping my 5 flush subs into my soil bin. I have been studying soil bacteria For awhile but I did not know B subtilis could stop Trich. I know why I go organic and it’s not just bc it’s better for the environment. It fosters life which...fosters MORE life go figure! Even if I have to pasteurize after seeing those spores explode, I’m using my cannabis soil. I bet the wood lovers would devour it. Sooo many roots in there. I’ll see if I can get a good pic of it now.
The grey stuff you can see really good to the left was healthy mycelium until it dried. The soil in there is pretty dry. To the right it a spent sub. The grey is all over but it’s dark and hard to see http://
-------------------- Literally, sever nober!
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: Egohst]
#27084948 - 12/12/20 01:54 AM (3 years, 1 month ago) |
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I don't know if B subtilis can stop trich but it can def stop cube myc alright and contaminate any grow. That's why we go to great lengths trying to kill its endospores in the grain we use for spawn.
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Lemgrub



Registered: 04/02/18
Posts: 589
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Re: Cultivation General Discussion [Re: Egohst]
#27084949 - 12/12/20 01:56 AM (3 years, 1 month ago) |
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Found a ton of quart jars at target today, and in my haste grabbed wide mouth. Luckily in the past I did the same with lids. Did a few PC runs of RGS.
Also ordered bags that arrived today so might as well PC them tomorrow and inoc with LC the next day.
Found a website offering millet for <¢69 a pound, so I may try that. Also interested in rice. Would probably be the most convenient by far
-------------------- We getting that pirate bay, alien shishkebab cordycep money

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coversall
إِنْ شَاءَ ٱللَهُ



Registered: 06/06/20
Posts: 2,749
Loc: संसार
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Re: Cultivation General Discussion [Re: Lemgrub]
#27085012 - 12/12/20 04:19 AM (3 years, 1 month ago) |
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Any viable alternative to peat moss when casing pans? I tried straight verm but didn't get good results, however, there is a large change that was due to my spawn.
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Sankhara
Trump's lost child


Registered: 02/11/18
Posts: 546
Loc: Argentina
Last seen: 10 days, 14 hours
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Re: Cultivation General Discussion [Re: Sankhara]
#27085024 - 12/12/20 04:35 AM (3 years, 1 month ago) |
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Quote:
Sankhara said: Hi people, i have a question.
I keep seeing threads about how APE spores not always grow APE's, and how cloning is generally necessary to get a consistent grow of ape characteristics and not cubes. Also it appears that its becoming more and more frequent.
My question is: is there a way to get to stabilize the mutation once again?
Anybody?
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Schemenhaft
Oachkatzlschwoaf



Registered: 01/14/17
Posts: 339
Loc: Right side of the big poo...
Last seen: 2 years, 8 months
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Re: Cultivation General Discussion [Re: Sankhara]
#27085051 - 12/12/20 05:18 AM (3 years, 1 month ago) |
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Quote:
Sankhara said:
Quote:
Sankhara said: Hi people, i have a question.
I keep seeing threads about how APE spores not always grow APE's, and how cloning is generally necessary to get a consistent grow of ape characteristics and not cubes. Also it appears that its becoming more and more frequent.
My question is: is there a way to get to stabilize the mutation once again?
Anybody?
Not 100% sure that helps but yesterday I was looking for a similar case and found this post.
Quote:
cronicr said: you're all wrong...sort of. You want both A and B. Cloning is a good way to get the genetics but the op says stabilized which is done over genrerations not just simply cloning so you would indeed first clone the fruit and fruit that, take spores from that grow and fruit that, spot the same trait and clone that...repeat
________________________________________________________________________________ Is there a legit way to "produce" PE-Fruits without having PE genetics? Something like crossing two strains or provoke an abortion like this?
At my current level of knowledge I think this is close to impossible with maximum a small random possibility to get something like PE, but maybe I just havent read about something or so. Aksing because it seems like impossible currently to get genetics of PE/APE here in europe, even all vendors from the list here currently stopped shipping to europe because of corona.
Maybe someone has an idea with a realistic chance of success, even when its hard work I would give it a try, need to cultivate these guys.
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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 14 days, 3 hours
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Re: Cultivation General Discussion [Re: tedoro]
#27085063 - 12/12/20 05:28 AM (3 years, 1 month ago) |
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Quote:
tedoro said: I recently made some cement pucks, about the size of a hockey puck... to set atop my dishes. one for every dish. Haven't tried them yet. I made them when I realized the trick is to have the thing on the top cool slower than the agar plate itself. My oven warmed pucks achieve that.
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Sankhara
Trump's lost child


Registered: 02/11/18
Posts: 546
Loc: Argentina
Last seen: 10 days, 14 hours
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Re: Cultivation General Discussion [Re: Schemenhaft]
#27085070 - 12/12/20 05:42 AM (3 years, 1 month ago) |
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Thanks for answering!
So, in theory cloning and sporulating over a few generations should work?
I dont know at what F (generations) APE spores may be at right now, but i thought that after f8/f9 there wasnt much to be done due to the small genetical variability that stable varieties present
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Schemenhaft
Oachkatzlschwoaf



Registered: 01/14/17
Posts: 339
Loc: Right side of the big poo...
Last seen: 2 years, 8 months
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Re: Cultivation General Discussion [Re: Sankhara]
#27085525 - 12/12/20 11:17 AM (3 years, 1 month ago) |
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I've found some ~3years old Agar-dishes with full mycelium, seems like dead. Is it possible to reactivate that mycelium or something without a pin on it? Think not possible, but maybe someone made other experiences.
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One of Us
Stranger



Registered: 03/12/12
Posts: 1,140
Last seen: 7 days, 18 hours
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Re: Cultivation General Discussion [Re: Schemenhaft] 1
#27085563 - 12/12/20 11:36 AM (3 years, 1 month ago) |
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Just transfer some out and see for yourself. You could be surprised.
I had a bunch of plates that froze solid for at least a few days due to refrigerator error. 80% of them recovered.
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starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 18 days
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Re: Cultivation General Discussion [Re: One of Us] 1
#27085626 - 12/12/20 12:07 PM (3 years, 1 month ago) |
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That makes me wonder. I got some plates from a buddy they don't look like they grew out, maybe the faintest hit of myc. I'm wondering if I can recover them on fresh agar.
The plates weren't even that old. Not sure what happened to them but they did not survive. Shame it was PE and APE too.
-------------------- Listen, I'm steel fisted with the iron lung Heavy metal ballads out the guitar where lions run.
 
Edited by starbones (12/12/20 02:00 PM)
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sh4d0ws
LSx


Registered: 02/26/08
Posts: 12,086
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Re: Cultivation General Discussion [Re: Schemenhaft] 1
#27085727 - 12/12/20 01:04 PM (3 years, 1 month ago) |
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Quote:
Schemenhaft said: I've found some ~3years old Agar-dishes with full mycelium, seems like dead. Is it possible to reactivate that mycelium or something without a pin on it? Think not possible, but maybe someone made other experiences.
Are they completely dried out? Try swabbing it with a sterile swab or a loop and put it onto a new plate and see what happens. It might be possible to revive it.
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GrinchGrower
N00B



Registered: 10/02/20
Posts: 682
Last seen: 2 years, 8 months
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Re: Cultivation General Discussion [Re: sh4d0ws]
#27085985 - 12/12/20 03:30 PM (3 years, 1 month ago) |
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I got too stoned and forgot to put my stir bars in LC bottles before sterilizing. I can whip up a new batch, but figured I'd try to save them.
Can I open them, insert the bars, and sterilize again for 15m @ 15 PSI or will this risk caramelizing the Malt Extract?
Would it be best to let them get down to room temperature beforehand or just turn around and do it while they are still hot?
Didn't want to make a new thread to ask...
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starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 18 days
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Re: Cultivation General Discussion [Re: GrinchGrower]
#27085988 - 12/12/20 03:33 PM (3 years, 1 month ago) |
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Sterilize the bars in a PC, just wrap them in foil and toss them in a jar. Fairly sure you can do dry heat sterilization on those too if need be. Crack the jars in your sab or flowhood and pop them in.
Probably be safer to pick them up with a heated scalpel blade as if you were doing a transfer.
I've forgot my stir bars before and just made new LC. It's cheap.
-------------------- Listen, I'm steel fisted with the iron lung Heavy metal ballads out the guitar where lions run.
 
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GrinchGrower
N00B



Registered: 10/02/20
Posts: 682
Last seen: 2 years, 8 months
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Re: Cultivation General Discussion [Re: starbones]
#27085991 - 12/12/20 03:37 PM (3 years, 1 month ago) |
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Thanks dude. It's my first attempt so I don't want to fuck it up anymore than I already have... 
I'll just make a new batch I reckon it doesn't take too long.
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Failboat
Fuck Up
Registered: 02/01/18
Posts: 8,736
Last seen: 11 hours, 56 minutes
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Re: Cultivation General Discussion [Re: GrinchGrower]
#27086012 - 12/12/20 04:02 PM (3 years, 1 month ago) |
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Open add and redo
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eLeSDenes
Mycelium Expander




Registered: 05/25/11
Posts: 955
Last seen: 1 year, 11 months
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Re: Cultivation General Discussion [Re: Failboat]
#27086343 - 12/12/20 07:31 PM (3 years, 1 month ago) |
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LC's are ridiculously easy to fuck up. Just start over, ingredients are cheap, your time is not
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The Fresh Prints
Smell ya later



Registered: 05/19/12
Posts: 1,377
Loc: Bel-Air
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Re: Cultivation General Discussion [Re: Failboat]
#27086362 - 12/12/20 07:53 PM (3 years, 1 month ago) |
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Quote:
Quirkmeister92 said: Open add and redo
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BrownBear
Warrior-Traveler



Registered: 06/05/20
Posts: 1,539
Last seen: 8 months, 7 days
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I've forgotten to put stir bars in my lc's once. I just let them cool down, added the stir bars and pc'd again. I don't use malt extact but I doubt it would make a diference.
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