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InvisiblePastywhyteMDiscord
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Re: Cultivation General Discussion [Re: Edmunter] * 1
    #24051106 - 01/30/17 09:31 AM (7 years, 13 hours ago)

Quote:

Edmunter said:
Can an isolate plate produce pins?




Of course. I would not even worry about isolating clones. Just grab healthy growth. Most clone colonies are going to produce very similar results anyways. Of course there is the possibility of some genetic drift but IME such occurences will be the exception rather than the rule.


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InvisibleJosex
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Re: Cultivation General Discussion [Re: Edmunter]
    #24051744 - 01/30/17 01:19 PM (7 years, 10 hours ago)

Quote:

Edmunter said:
Can an isolate plate produce pins?



Fuck, that hurts dud.


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OfflineAlCapone2k
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Re: Cultivation General Discussion [Re: Josex] * 1
    #24052298 - 01/30/17 05:00 PM (7 years, 6 hours ago)

I think he meant it different than we understood it. Edmunter is not a noob anymore, I think it's a missunderstanding.


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


Edited by AlCapone2k (01/30/17 05:00 PM)


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OfflineKenetic
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Re: Cultivation General Discussion [Re: AlCapone2k]
    #24052311 - 01/30/17 05:07 PM (7 years, 6 hours ago)

How to tell if an ape spore swab is successful? 

My guess is either a microscope or an agar swipe, but I'd like to know before I made a bunch of them.  Any other way?


--------------------
Todo Cambia
   

               
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OfflineFunGuyZ
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Re: Cultivation General Discussion [Re: Kenetic]
    #24052344 - 01/30/17 05:22 PM (7 years, 6 hours ago)

Liquid culture is pretty much just 4% starch/sugar by weight no matter what you use correct?  Some use pd/brf, dme, honey/karo, grain water, etc. What works best for you and why do you go with it?


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InvisibleComebackKid
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Re: Cultivation General Discussion [Re: FunGuyZ]
    #24052587 - 01/30/17 06:54 PM (7 years, 4 hours ago)

You don't wanna use simple sugars like karo or hone. .. or sugar:lol:
They make for weak cultures

Use something with higher nutrients for more aggressive growth


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:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
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is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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OfflineFunGuyZ
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Re: Cultivation General Discussion [Re: ComebackKid]
    #24052606 - 01/30/17 07:02 PM (7 years, 4 hours ago)

Guess I should have said sweetener. Didn't mean actual sugar. I consider them sugars because sugar is glucose, sucrose, etc. It's a bunch term for the science in my mind. So malt, pd/brf is the way to go then.


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InvisibleComebackKid
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Re: Cultivation General Discussion [Re: FunGuyZ]
    #24052619 - 01/30/17 07:06 PM (7 years, 4 hours ago)

I guess malt would also be considered a sugar but yes brf and potatoe flakes are definitely the way to go


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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OfflineThedillestpickle
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Re: Cultivation General Discussion [Re: ComebackKid]
    #24052988 - 01/30/17 09:20 PM (7 years, 2 hours ago)

I took a tranfer from a plate of growing out spores and I zoomed in really close and just nabbed off the tinniest sliver of a filament of growth, the so-called "invisible" growth.  Put it to a plate and I got an isolate in one transfer... it's probably a shit isolate though, super tomentose, but I just put it to grain yesterday so will see what happens.


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: Thedillestpickle]
    #24052991 - 01/30/17 09:22 PM (7 years, 2 hours ago)

no pics of your isolate? and 1 transfer? but no pics?

:jolly:


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OfflineThedillestpickle
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Re: Cultivation General Discussion [Re: mushboy]
    #24053025 - 01/30/17 09:34 PM (7 years, 1 hour ago)



here it is... kinda grown out though, so you can't see much.  The leading growth was a uniform circle and there are knots forming that all line up with equal radius from the center of growth. Plus it's all equally completely white and uniform.  pretty terrible photo though


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OfflineThedillestpickle
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Re: Cultivation General Discussion [Re: Thedillestpickle]
    #24053026 - 01/30/17 09:35 PM (7 years, 1 hour ago)

Oh yea and the lumps are because I was busy while my agar was cooling and let it get clumpy... worst agar pour ever


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: Thedillestpickle]
    #24053043 - 01/30/17 09:42 PM (7 years, 1 hour ago)

what makes you think its an isolate?

this fits your definition but it aint no isolate


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OfflineKenetic
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Re: Cultivation General Discussion [Re: mushboy]
    #24053208 - 01/30/17 10:45 PM (7 years, 38 minutes ago)

Looks like an overgrown plate


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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InvisibleInocuole
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Re: Cultivation General Discussion [Re: AlCapone2k]
    #24053219 - 01/30/17 10:51 PM (7 years, 33 minutes ago)

Quote:

AlCapone2k said:
I think he meant it different than we understood it. Edmunter is not a noob anymore, I think it's a missunderstanding.




So you say...


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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InvisibleMoabfighter
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Re: Cultivation General Discussion [Re: Inocuole]
    #24053262 - 01/30/17 11:14 PM (7 years, 10 minutes ago)

Not cult, yet, sorry. Next post will be. Trying to clear enough phone memory to take a pic of my most recent exciting doings this evening.

Mega stoned and was thinking. Everything is either a plant or animal. Except a damn mushroom.  It's neither, correct? I guess a rock wouldn't be either. But I mean, you know what I mean....


Growing mushrooms. What the hell is a mushroom even?

Crazy shit man


--------------------
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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: Moabfighter]
    #24053271 - 01/30/17 11:18 PM (7 years, 6 minutes ago)

they are fun

fungi. they dont call it 'flesh of the gods' for no reason.

pass that shit this way.

my guy is being threaten with deportation back to somilia. fakk. its getting personal now.
1st world real white people problems yo. shit just got real.


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InvisibleMoabfighter
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Re: Cultivation General Discussion [Re: Thedillestpickle]
    #24053276 - 01/30/17 11:20 PM (7 years, 4 minutes ago)

Quote:

Thedillestpickle said:


here it is... kinda grown out though, so you can't see much.  The leading growth was a uniform circle and there are knots forming that all line up with equal radius from the center of growth. Plus it's all equally completely white and uniform.  pretty terrible photo though





Also, before we rule this guy out. It sounds like he's confident in what he's saying. Which to me means this new person may have done their research. I've not looked at his other posts, maybe you all have, but I've been doing agar for a decent few months now, and am 1000% sure I do not have an isolate, nor am even close (although, I've kept the same culture going since I started agar, 6th transfer KSSS plate in the fridge, did 4 plates of 7th transfer)

Anyway, maybe this guy nailed something. I personally always grab the tiniest, straightest singular strand of myc I can possibly slice, and transfer that. My 7th plate now may be on its way to an isolate, but who knows really.

I don't know exactly whalers I was going with this but you may get the idea.


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KSSS And PE WBS.
 


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InvisiblePastywhyteMDiscord
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Re: Cultivation General Discussion [Re: Moabfighter]
    #24053287 - 01/30/17 11:24 PM (7 years, 7 seconds ago)

He is wrong. A single hyphae is microscopic.


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InvisibleMoabfighter
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Re: Cultivation General Discussion [Re: Pastywhyte]
    #24053300 - 01/30/17 11:31 PM (6 years, 11 months ago)

Quote:

Pastywhyte said:
He is wrong. A single hyphae is microscopic.





Makes sense. Nailed it.

My honest thought taking a teeny tiny spot of myc would be billions of genes, to millions of genes, which is think would be better, but i could also be wrong on that too.

I try to be precise and think nice and hard about the movies I make. I would think the prettiest finest smallest piece, from the nicest most uniform centimeter of mycelium, would lead to more optimal results. This isn't to even talk about isolate guy above, but am I wasting my time trying to take small minute wedges for transfers bs a big chunk of many myc strands?

Thanks and sorry to trail.

To add a little more to what I'm saying, here's 5th transfer from the smallest bit I can get. This was a month ago.



I don't know if looks and appearance count for anything, but that looks nice to me.


--------------------
KSSS And PE WBS.
 


Edited by Moabfighter (01/30/17 11:45 PM)


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