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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Cultivation General Discussion [Re: Edmunter] 1
#24051106 - 01/30/17 09:31 AM (7 years, 13 hours ago) |
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Quote:
Edmunter said: Can an isolate plate produce pins?
Of course. I would not even worry about isolating clones. Just grab healthy growth. Most clone colonies are going to produce very similar results anyways. Of course there is the possibility of some genetic drift but IME such occurences will be the exception rather than the rule.
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: Edmunter]
#24051744 - 01/30/17 01:19 PM (7 years, 10 hours ago) |
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Quote:
Edmunter said: Can an isolate plate produce pins?
Fuck, that hurts dud.
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Cultivation General Discussion [Re: Josex] 1
#24052298 - 01/30/17 05:00 PM (7 years, 6 hours ago) |
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I think he meant it different than we understood it. Edmunter is not a noob anymore, I think it's a missunderstanding.
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
Edited by AlCapone2k (01/30/17 05:00 PM)
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: Cultivation General Discussion [Re: AlCapone2k]
#24052311 - 01/30/17 05:07 PM (7 years, 6 hours ago) |
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How to tell if an ape spore swab is successful?
My guess is either a microscope or an agar swipe, but I'd like to know before I made a bunch of them. Any other way?
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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FunGuyZ
Psilly Rabbit


Registered: 12/13/16
Posts: 177
Loc: down the rabbit hole
Last seen: 4 years, 9 months
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Re: Cultivation General Discussion [Re: Kenetic]
#24052344 - 01/30/17 05:22 PM (7 years, 6 hours ago) |
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Liquid culture is pretty much just 4% starch/sugar by weight no matter what you use correct? Some use pd/brf, dme, honey/karo, grain water, etc. What works best for you and why do you go with it?
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Cultivation General Discussion [Re: FunGuyZ]
#24052587 - 01/30/17 06:54 PM (7 years, 4 hours ago) |
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You don't wanna use simple sugars like karo or hone. .. or sugar They make for weak cultures
Use something with higher nutrients for more aggressive growth
--------------------
Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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FunGuyZ
Psilly Rabbit


Registered: 12/13/16
Posts: 177
Loc: down the rabbit hole
Last seen: 4 years, 9 months
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Re: Cultivation General Discussion [Re: ComebackKid]
#24052606 - 01/30/17 07:02 PM (7 years, 4 hours ago) |
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Guess I should have said sweetener. Didn't mean actual sugar. I consider them sugars because sugar is glucose, sucrose, etc. It's a bunch term for the science in my mind. So malt, pd/brf is the way to go then.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Cultivation General Discussion [Re: FunGuyZ]
#24052619 - 01/30/17 07:06 PM (7 years, 4 hours ago) |
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I guess malt would also be considered a sugar but yes brf and potatoe flakes are definitely the way to go
--------------------
Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Thedillestpickle
cultured



Registered: 02/02/16
Posts: 1,170
Loc: Canada
Last seen: 3 years, 7 months
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Re: Cultivation General Discussion [Re: ComebackKid]
#24052988 - 01/30/17 09:20 PM (7 years, 2 hours ago) |
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I took a tranfer from a plate of growing out spores and I zoomed in really close and just nabbed off the tinniest sliver of a filament of growth, the so-called "invisible" growth. Put it to a plate and I got an isolate in one transfer... it's probably a shit isolate though, super tomentose, but I just put it to grain yesterday so will see what happens.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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no pics of your isolate? and 1 transfer? but no pics?
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Thedillestpickle
cultured



Registered: 02/02/16
Posts: 1,170
Loc: Canada
Last seen: 3 years, 7 months
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Re: Cultivation General Discussion [Re: mushboy]
#24053025 - 01/30/17 09:34 PM (7 years, 1 hour ago) |
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here it is... kinda grown out though, so you can't see much. The leading growth was a uniform circle and there are knots forming that all line up with equal radius from the center of growth. Plus it's all equally completely white and uniform. pretty terrible photo though
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Thedillestpickle
cultured



Registered: 02/02/16
Posts: 1,170
Loc: Canada
Last seen: 3 years, 7 months
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Oh yea and the lumps are because I was busy while my agar was cooling and let it get clumpy... worst agar pour ever
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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what makes you think its an isolate?
this fits your definition but it aint no isolate
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: Cultivation General Discussion [Re: mushboy]
#24053208 - 01/30/17 10:45 PM (7 years, 38 minutes ago) |
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Looks like an overgrown plate
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Cultivation General Discussion [Re: AlCapone2k]
#24053219 - 01/30/17 10:51 PM (7 years, 33 minutes ago) |
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Quote:
AlCapone2k said: I think he meant it different than we understood it. Edmunter is not a noob anymore, I think it's a missunderstanding.
So you say...
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Moabfighter
Tam Fighter



Registered: 12/13/15
Posts: 2,710
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Re: Cultivation General Discussion [Re: Inocuole]
#24053262 - 01/30/17 11:14 PM (7 years, 10 minutes ago) |
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Not cult, yet, sorry. Next post will be. Trying to clear enough phone memory to take a pic of my most recent exciting doings this evening.
Mega stoned and was thinking. Everything is either a plant or animal. Except a damn mushroom. It's neither, correct? I guess a rock wouldn't be either. But I mean, you know what I mean....
Growing mushrooms. What the hell is a mushroom even?
Crazy shit man
-------------------- KSSS And PE WBS.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: Moabfighter]
#24053271 - 01/30/17 11:18 PM (7 years, 6 minutes ago) |
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they are fun
fungi. they dont call it 'flesh of the gods' for no reason.
pass that shit this way.
my guy is being threaten with deportation back to somilia. fakk. its getting personal now. 1st world real white people problems yo. shit just got real.
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Moabfighter
Tam Fighter



Registered: 12/13/15
Posts: 2,710
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Quote:
Thedillestpickle said:

here it is... kinda grown out though, so you can't see much. The leading growth was a uniform circle and there are knots forming that all line up with equal radius from the center of growth. Plus it's all equally completely white and uniform. pretty terrible photo though
Also, before we rule this guy out. It sounds like he's confident in what he's saying. Which to me means this new person may have done their research. I've not looked at his other posts, maybe you all have, but I've been doing agar for a decent few months now, and am 1000% sure I do not have an isolate, nor am even close (although, I've kept the same culture going since I started agar, 6th transfer KSSS plate in the fridge, did 4 plates of 7th transfer)
Anyway, maybe this guy nailed something. I personally always grab the tiniest, straightest singular strand of myc I can possibly slice, and transfer that. My 7th plate now may be on its way to an isolate, but who knows really.
I don't know exactly whalers I was going with this but you may get the idea.
-------------------- KSSS And PE WBS.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Cultivation General Discussion [Re: Moabfighter]
#24053287 - 01/30/17 11:24 PM (7 years, 7 seconds ago) |
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He is wrong. A single hyphae is microscopic.
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Moabfighter
Tam Fighter



Registered: 12/13/15
Posts: 2,710
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Re: Cultivation General Discussion [Re: Pastywhyte]
#24053300 - 01/30/17 11:31 PM (6 years, 11 months ago) |
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Quote:
Pastywhyte said: He is wrong. A single hyphae is microscopic.
Makes sense. Nailed it.
My honest thought taking a teeny tiny spot of myc would be billions of genes, to millions of genes, which is think would be better, but i could also be wrong on that too.
I try to be precise and think nice and hard about the movies I make. I would think the prettiest finest smallest piece, from the nicest most uniform centimeter of mycelium, would lead to more optimal results. This isn't to even talk about isolate guy above, but am I wasting my time trying to take small minute wedges for transfers bs a big chunk of many myc strands?
Thanks and sorry to trail.
To add a little more to what I'm saying, here's 5th transfer from the smallest bit I can get. This was a month ago.

I don't know if looks and appearance count for anything, but that looks nice to me.
-------------------- KSSS And PE WBS.
Edited by Moabfighter (01/30/17 11:45 PM)
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