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Offlinestarbones
I'm an alien, I eat uranium.
I'm a teapot


Registered: 03/04/20
Posts: 1,131
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Re: Cultivation General Discussion [Re: Wall.E]
    #27056091 - 11/24/20 07:33 PM (3 years, 2 months ago)

I wasn't going to label these was going to use food coloring and a dry erase board to keep track of things but then I remembered I have a bunch of LC from the other day I thought it would look cute if I made all the colours of a stop light and well that's fucked my system.

I used to just use a crayon to write a letter and the day on a metal lid if I know I'm using the LC that month and the letter to remind me which var it is. That system pittered out when I lost my crayon and I CBF to buy a whole pack no matter how good they taste.


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Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.




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OfflineYeetusdeetus
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Re: Cultivation General Discussion [Re: sh4d0ws]
    #27056120 - 11/24/20 07:48 PM (3 years, 2 months ago)

Quote:

sh4d0ws said:
Quote:

tryptkaloids said:
I have a buddy who even stores them in the freezer




Colonized spawn in the freezer?

I thought freezing temps kill cube mycelium

FWIW I have many lids made from cut-up SFD's that have been used for plenty of PC cycles..they work great. Unmodded plastic lids work too. Metal lids get rusty fast IME





Quote:

cronicr said:
It's not getting cubes to fruit in cold weather that would be the problem.
In order to survive the year the spores will need to live through freezing:shrug:




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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: Yeetusdeetus]
    #27056125 - 11/24/20 07:52 PM (3 years, 2 months ago)

I got little colored dot stickers and use a different color for different varieties. And I can date it and write an identifier on (ms/clone)


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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Learn the tried and true way to grow mushrooms
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InvisibleWall.E
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #27056134 - 11/24/20 07:55 PM (3 years, 2 months ago)

We all got tricks eh. Anything beats the one round my dumbass did writing everything. After the first 5 I finally shortened psilocybe to "p."

This is the shit I'm talking about when I say I'm an idiot guys. Like sometimes common sense just fucking bounces out of my life. I'm not saying I'm dumb, like I learn shit well, but also do really stupid shit like that.


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Life’s shit, but I’m loving it



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OfflineOvoidhunter
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Re: Cultivation General Discussion [Re: Wall.E]
    #27056322 - 11/24/20 09:47 PM (3 years, 2 months ago)

What is senescence? How do I avoid it?


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InvisiblefahtsterM
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Re: Cultivation General Discussion [Re: Ovoidhunter]
    #27056334 - 11/24/20 09:54 PM (3 years, 2 months ago)

Myc stops fruiting... don’t clone clones over and over again.. when you get a culture you like, put it to a slant and keep it in the fridge.. when the culture looks like it’s getting tired, go back to the slant and run it from the slant again... also changing up grains that you use will help

Faht


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Invisiblesh4d0ws
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Registered: 02/26/08
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Re: Cultivation General Discussion [Re: fahtster]
    #27056339 - 11/24/20 09:58 PM (3 years, 2 months ago)

For labeling jars I just use a sharpie and write directly on the glass. It's how we do it in the lab for glassware as well.

Acetone or isopropyl soaked tissue/paper towel and it wipes off easily when the jar is finished or whatever.

Also - I use industrial sharpies for my petri dishes...best thing ever, no more rubbing off your labels when you wipe the plates down before a transfer. I've heard red sharpies work for this too but the industrial high temp resistant sharpies are just perfect for petri labeling.

these things - https://www.amazon.ca/Sharpie-INDUSTRIAL-Marker-Permanent-13601/dp/B00006IFEO/ref=sr_1_3?dchild=1&keywords=industrial+sharpie&qid=1606280427&sr=8-3


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: Ovoidhunter]
    #27056348 - 11/24/20 10:02 PM (3 years, 2 months ago)

Yeah it’s basically when you run the same culture over and over and expand the myc out too many times it starts to get old and tired. It takes a long time though so it’s not something you’ll run into often unless you’re only running one culture and totally mismanaging the expansions.

Like faht said slants and masters come into play if that’s the type of thing you’re planning on doing.


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LAGM2020


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OfflineOvoidhunter
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Re: Cultivation General Discussion [Re: A.k.a]
    #27056392 - 11/24/20 10:50 PM (3 years, 2 months ago)

Definitely gonna pop a master in the fridge now. I thought my mycelium was looking a little weak on agar but I've been pulling 5-6oz per tub just like the first time I ran this culture.. I'm only on the third grow with it.


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InvisibleWall.E
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Re: Cultivation General Discussion [Re: Ovoidhunter]
    #27056590 - 11/25/20 05:23 AM (3 years, 2 months ago)

Would a larger surface area allow for more pins? Some guy was asking about cvg and I was thinking that by adding verm you could retain a bit of moisture and it could allow for a larger surface area with its rough and small shapes but I would think that it would be negligible.


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Life’s shit, but I’m loving it



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Offlinestarbones
I'm an alien, I eat uranium.
I'm a teapot


Registered: 03/04/20
Posts: 1,131
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Re: Cultivation General Discussion [Re: Wall.E]
    #27056807 - 11/25/20 09:28 AM (3 years, 2 months ago)

My muda-style bottle surfaces are drying out on me in pasty-ez dials (the hole not mp kind)

Heck.


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Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.




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OfflineeLeSDenes
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Re: Cultivation General Discussion [Re: starbones]
    #27056861 - 11/25/20 10:03 AM (3 years, 2 months ago)

Guys, I just got some nice swabs so I made a few plates streaking the agar with the swab. I tracked the streak lines with a microscope and only found maybe a few spores scattered across. I think the spores are fresh but don't see much chance of  them  germinating. Is there any hack to leave a bit more spores when using a swab?


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InvisibleWall.E
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Re: Cultivation General Discussion [Re: eLeSDenes] * 1
    #27056884 - 11/25/20 10:16 AM (3 years, 2 months ago)

Just stick it in


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Life’s shit, but I’m loving it



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OfflineCamera93
We got dicks like Jesus
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Re: Cultivation General Discussion [Re: Wall.E]
    #27056912 - 11/25/20 10:33 AM (3 years, 2 months ago)

:whathesaid:


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All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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Offlinestarbones
I'm an alien, I eat uranium.
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Re: Cultivation General Discussion [Re: Camera93]
    #27056926 - 11/25/20 10:41 AM (3 years, 2 months ago)

To get my Melmak swab to germ I used a sterile 22g syringe to grab some cotton off the swab in front of my flowhood then using the needle pulled the tuft covered in spore through the agar in a big line.

Repeated it across 4 plates using new tufts of cotton from the end of the swab. Each chunk of cotton ended up buried in the agar.


--------------------
Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.




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Offlinesmalltalk_canceled
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Re: Cultivation General Discussion [Re: starbones]
    #27056980 - 11/25/20 11:16 AM (3 years, 2 months ago)

https://psychedelicreview.com/catching-up-with-the-psychedelic-entourage-effect-part-1-how-we-got-here/

"Aeruginascin seems to modify the pharmacological action of psilocybin to give an always euphoric mood during the ingestion of mushrooms."


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Willpower is the one true virtue



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InvisibleWall.E
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Re: Cultivation General Discussion [Re: smalltalk_canceled]
    #27057090 - 11/25/20 12:49 PM (3 years, 2 months ago)

I don't click on links, but does that talk about how that chemical is suspect for causing woodlovers paralysis?


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Life’s shit, but I’m loving it



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InvisibleReverendMyc

Registered: 03/29/19
Posts: 1,508
Re: Cultivation General Discussion [Re: Wall.E]
    #27057098 - 11/25/20 12:57 PM (3 years, 2 months ago)

Nope: copy paste below. I would summarize as experts disagree on whether other compounds in mushrooms have an enhancing effect on the psilocin.


Catching Up With the Psychedelic Entourage Effect-Part 1: How We Got Here
The entourage effect caught PSR’s eye back in early 2019. What are experts saying about the theory now and how are psychedelic research companies approaching it?

NOVEMBER 25, 2020 - BY BARBARA E. BAUER, MS

In early 2019, Psychedelic Science Review published an article titled “The Entourage Effect in Magic Mushrooms.” The article summarized three scholarly papers which brought together the first clues that the entourage effect could play a role in the effects of psychedelic mushrooms. One of the studies was conducted decades ago by the famous mushroom researcher Jochen Gartz.1 He hinted at the entourage effect in his data analysis from reports on accidental mushroom ingestion. Some of the mushrooms contained the compound aeruginascin, while others did not. Gartz observed that,

Aeruginascin seems to modify the pharmacological action of psilocybin to give an always euphoric mood during the ingestion of mushrooms.

Further investigation by researchers has yielded additional evidence suggesting there may be a synergistic relationship between the compounds in magic mushrooms and other organisms.

Finding More Active Compounds
In 1968, researchers Albert Leung and AG Paul isolated the compounds baeocystin and norbaeocystin from the magic mushroom Psilocybe baeocystis.2 Since then, scientists have discovered other active compounds in magic mushrooms beside the more familiar psilocybin and its active metabolite psilocin. For example, in 2017, Lenz et al. first identified the compound norpsilocin in Psilocybe cubensis.3 A study by Sherwood et al. in 2020 found that norpsilocin was not only active at the serotonin 5-HT2A receptor but was more potent than psilocin using a calcium flux assay.4

In November 2019, Blei et al. reported that they had discovered compounds called ß-carbolines in four Psilocybe species.5 ß-carbolines are naturally occurring alkaloids more commonly known for their presence in the psychotropic beverage ayahuasca. Several ß-carbolines inhibit monoamine oxidase enzymes (MAOs). Without co-administering ß-carbolines, MAOs would break down the DMT (dimethyltryptamine) in the ayahuasca too rapidly for it to produce significant biological effects for the user. Blei et al. summarized the case for ß-carbolines participating in an analogous entourage effect in magic mushrooms, explaining,

We conclude that Psilocybe mushrooms produce an ayahuasca-like and potentially similarly synergistic set of metabolites that may impact upon onset and duration of their effects.

What the Mushroom Experts Are Saying
In a recent interview, psychedelic mushroom expert Paul Stamets told Joe Rogan that the “wave of the future” is making standardized formulations that include multiple psilocybin analogs, not just psilocybin. This is because combining multiple psilocybin analogs provides what Stamets calls “an entourage or symphony effect” that is not present with single active ingredients.

In April 2020, the iconic researcher, documentarian, writer, and psychonaut Hamilton Morris commented to Psychedelic Science Review in an email about the entourage effect in toad secretions. Specifically, we asked him about differences in effects between whole told secretions versus pure 5-MeO-DMT. Morris said, “The differences are widely reported in the psychedelic community, but are not based on anything approaching a controlled double-blind experiment with a large number of subjects, which would be required to meaningfully establish a difference between the two materials.”

Speaking again about toad secretions, Morris told Joe Rogan in his podcast,

…maybe there’s a little bit of that sort of entourage effect that you get with almost any plant that has a variety of different alkaloids.

So, there are some conflicting opinions about the entourage effect among at least two magic mushroom experts.

What the Pharmacologists Are Saying
Psychedelic Science Review reached out to psychedelic researchers Dr. David Nichols and Dr. Alexander Sherwood for their comments on the state of the entourage effect hypothesis in psychedelic compounds. Regarding magic mushrooms, Dr. Nichols told us,

I do not believe there is an entourage effect for mushrooms. The other two major tryptamines in mushrooms, baeocystin and norbaeocystin, have recently been synthesized and tested and they are not active.[4] Pure synthetic psilocybin seems to represent the essential effect of the whole mushroom. I believe any entourage effect would be very subtle, if any.

When asked about a synergy between the compounds in toad secretions (venom), he noted,

…when the dried toad venom is smoked, the peptides are destroyed by the heat. It is possible that there are other volatile compounds in the toxin, but I have not seen evidence that they contribute to the effect.

For comparison purposes, Dr. Nichols pointed out how research into cannabis compounds is at a more advanced stage than other psychedelics. “Cannabis contains hundreds of compounds, most of which have not been tested. ∆9-THC is the main psychoactive component in cannabis, but it also contains ∆8-THC, cannabidiol, and a variety of terpenes and cannabinoids. It is possible that the overall effect of ingesting cannabis is a combination of its components, or an entourage effect.”

To Dr. Nichols’s point, research is indicating that there is a synergy between some of the cannabinoids found in the Cannabis plant.6,7

Dr. Alexander Sherwood told Psychedelic Science Review, “I am excited to see that there are groups actively working to elucidate this interesting and complex phenomenon.” He offered these additional thoughts,

At this time, I have not seen strong evidence to support the idea [the entourage effect] one way or another. It is tempting to make an analogy between the effects produced by mixtures of lipophilic phytocannabinoids in cannabis and the various alkaloids in psychedelic mushrooms, peyote, or toad secretions, but none are directly comparable.

Looking ahead, Dr. Sherwood added,

The important thing to do now is design good experiments to test the entourage effect hypothesis systematically with representative mixtures of natural products.

He continued, “Discovering that a compound such as norpsilocin is an agonist at the 5-HT2A receptor is a very small piece of the puzzle. Understanding the absorption, distribution, metabolism, and excretion characteristics of the individual components in the mixture is equally, if not more, important to consider.”

There are two conclusions one can glean from this expertise provided by Drs. Nichols and Sherwood. First, there is significant skepticism among psychedelic pharmacologists about the existence of the entourage effect in psychedelic compounds (aside from cannabis). This makes sense due to the lack of proper scientific studies.

Second, it would be helpful for researchers to work toward developing a better understanding of the complete chemical composition of naturally occurring psychedelic sources like magic mushrooms (or toad secretions, ayahuasca, Salvia divinorum, etc.). This requires first identifying all the compounds they contain and understanding their chemistry and pharmacology. Then, studies can be designed to help understand how two or more of the molecules may work together to produce the entourage effect.

Examining the Work of Psychedelic Research Companies
Understandably, many experts remain unconvinced that there is a synergistic relationship between psychedelic compounds except when it comes to Cannabis. But how are commercial psychedelic research companies approaching the entourage effect? Are they treating it as an untapped opportunity or a red herring?

Over the coming weeks, this article series will review several companies that appear to be actively studying the entourage effect in their research efforts. We will summarize what they’re working on and discuss what it could mean for the future of psychedelic research. Keeping an eye on where these companies are heading and the research strategies they are using is a critical part of staying up to date with psychedelic science.


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LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
Don't Panic




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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: ReverendMyc]
    #27057483 - 11/25/20 05:26 PM (3 years, 2 months ago)

I read that a while back on the original site, it’s definitely interesting that the groups getting the chemical had zero negative aspects.

This to me sounds a whole lot like how straight thc can induce anxiety but if you mix it with cbd it creates a whole new thing that’s much more relaxed.


I wonder how long until you can just order up capsules with custom blends of all the actives. Like how at dispensaries now you can get whatever % of cbd, thc, and individual terpenes.


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LAGM2020


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OfflineAtmozFear
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Re: Cultivation General Discussion [Re: A.k.a]
    #27057506 - 11/25/20 05:36 PM (3 years, 2 months ago)

Quote:

A.k.a said:
I wonder how long until you can just order up capsules with custom blends of all the actives. Like how at dispensaries now you can get whatever % of cbd, thc, and individual terpenes.



Really?  That’s incredibly technical to isolate individual terpenes... I didn’t even know it could be done.  Hold on, do you mean weed strains with different levels or some kind of capsule with all the oils?


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Edited by AtmozFear (11/25/20 05:38 PM)


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