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OfflineRumpleforeskin
Scientist new to mycology
Male


Registered: 07/05/20
Posts: 82
Loc: Canada Flag
Last seen: 3 years, 1 month
Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #27045493 - 11/18/20 12:40 PM (3 years, 2 months ago)

Quote:

p9hu7 said:
I wouldn't g2g anything inoculated with spore solution. Your plan seems overly optimistic. I don't know what makes you guys think that spores to grain equals more genetic diversity, and more opportunities.

I would prepare for contamination and disappointment. This plan relies on the assumption that you have clean syringes. You also assume that you're capable of maintaining these tubs without experience with fruiting tubs. It would appear as though you're putting th horse before the cart.

Cook up some agar, clean your cultures up. Put those cultures to grain, then spawn tubs. This is a good plan, your plan is full of holes and will likely waste your time and resources. I'm not trying to be pessimistic, what I'm saying is sound reasoning.

Just think it through. If you lack the experience to catch contamination on your grains then you'll be compounding your frustrations down the line with g2g.
Your syringes are definitely going to contaminate some percentage of your grain in some recognizable way, like stalling, trich, or visible bacteria. Those jars or bags will be tossed, you'll feel good about the remainder. Those may be infected as well but you just can't see it, so you g2g this bad spawn. Spawn run is complete, you spawn tubs, tubs take forever and then you get trich or a very sparse/shitty flush...this will cause you to generate threads asking "but why". This will waste time and it will discourage you.

Agar first. Then learn how to properly hydrate your grain because that's an entirely separate problem that people run into. Then learn how to successfully transfer from your plate to grain.
Once you do that you'll still have contamination but you'll be getting closer. Soo much for you to have to learn without having to add the uncertainty of spores to grain.





Thank you for your thoughtful and detailed response brother.  Agar program on full bore now :smile:
Any thoughts on number of transfers before I start needing to be concerned about the mycelium getting tired?  I'm up to T4 on a couple of varieties....How far can i push these before I need to start over with a TC from something or more MSS to agar?


Edited by Rumpleforeskin (11/18/20 12:44 PM)


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OfflineYeetusdeetus
 User Gallery

Registered: 11/23/19
Posts: 1,242
Last seen: 3 hours, 48 minutes
Re: Cultivation General Discussion [Re: Rumpleforeskin]
    #27045512 - 11/18/20 01:00 PM (3 years, 2 months ago)

Home growers don’t really need to worry about senescence. This’ll give you more context: https://www.shroomery.org/forums/showflat.php/Number/25911960


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OfflineA.k.aM
Stranger
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Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 2 hours, 28 minutes
Trusted Cultivator
Re: Cultivation General Discussion [Re: Yeetusdeetus]
    #27045560 - 11/18/20 01:41 PM (3 years, 2 months ago)

The sub shrinking away from the sides is normal, no reason to mist. The myc is eating it and pulling everything together tighter as it goes.


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LAGM2020


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Offlinesmalltalk_canceled
Babnik
 User Gallery

Registered: 07/13/20
Posts: 2,862
Last seen: 13 days, 7 hours
Re: Cultivation General Discussion [Re: A.k.a]
    #27045590 - 11/18/20 01:52 PM (3 years, 2 months ago)

Don't wanna disturb the super interesting conversation going on

Just wanna give a big shoutout to the Corona virus, these masks are a goodsend!



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Willpower is the one true virtue



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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 9 hours
Trusted Cultivator
Re: Cultivation General Discussion [Re: smalltalk_canceled] * 1
    #27045597 - 11/18/20 01:54 PM (3 years, 2 months ago)

A surgical mask doesn't seem like the best spawn filter.
Keep us updated on how well those work


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: Cultivation General Discussion [Re: smalltalk_canceled] * 1
    #27045615 - 11/18/20 02:02 PM (3 years, 2 months ago)

Quote:

smalltalk_canceled said:
Don't wanna disturb the super interesting conversation going on

Just wanna give a big shoutout to the Corona virus, these masks are a goodsend!





Oh honey..


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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Offlinestarbones
I'm an alien, I eat uranium.
I'm a teapot


Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 18 days
Re: Cultivation General Discussion [Re: Inocuole]
    #27045632 - 11/18/20 02:15 PM (3 years, 2 months ago)

The googler tells me that the pore size of a standard surgical mask is between 0.3-10um.

Don't think it's a great idea but hoping it works for you.
I used beading felt from the fabric store, 1 square meter for $10. Contam rate was really low. Micropore can eat my ass the residue it leaves behind and it shits up the lids.

Moved on to 1" SFDs now. Too cheap not to.


--------------------
Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.




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OfflineMLPismyOPSEC
That One Ponyfucker
I'm a teapot

Registered: 11/13/18
Posts: 884
Loc: Equestria? Mordor? Wester... Flag
Last seen: 11 days, 8 hours
Re: Cultivation General Discussion [Re: starbones]
    #27045844 - 11/18/20 04:17 PM (3 years, 2 months ago)

SFD's are so worth it, but shit, the mask material might work out just fine! I wouldn't bet my life savings but i'd have high hopes.


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InvisibleJosex
#cheat_code
 User Gallery

Registered: 11/13/15
Posts: 8,995
Loc: Flag
Trusted Cultivator
Re: Cultivation General Discussion [Re: MLPismyOPSEC]
    #27045866 - 11/18/20 04:26 PM (3 years, 2 months ago)

SFD's might be hard to find in his country unless he paids for an international order. On the other hand, polyester stuffing aka polyfill is readily available everyfuckingwhere. Awesome filter too, and cheap.


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Offlinestarbones
I'm an alien, I eat uranium.
I'm a teapot


Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 18 days
Re: Cultivation General Discussion [Re: MLPismyOPSEC]
    #27045875 - 11/18/20 04:28 PM (3 years, 2 months ago)

It very well could. I used beading felt and it's insanely air permeable. If I take a section of it and blow through it there is very little resistance because it's basically just flattened polyfil. I imagine the pore size is all over the place compared to micropore. Fuck micropore though I have so many lids that are fucked.

I wish, I wish those Mainstay plastic lids from Wal-Mart were sold in Canada. So damn inexpensive compared to the offerings here. Hobby mycology in Canada is fuuuuucking expensive sometimes and things can be difficult to find.


--------------------
Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.




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OfflineBrownBear
Warrior-Traveler
 User Gallery


Registered: 06/05/20
Posts: 1,539
Last seen: 8 months, 7 days
Re: Cultivation General Discussion [Re: starbones]
    #27045969 - 11/18/20 05:18 PM (3 years, 2 months ago)

I know a vendor that ships to Canada. They have the wide mouth and the regular mouth plastic jar lids. They are a better quality than you can find at walmart. The price isn't bad either. Or you could flip the metal jar lid so that it doesn't seal to the jar. I don't tighten them down all of the way just enough so that its not loose and then wrap the entire lid in parafilm. I wrap my pastic lids with parafilm also.



The only lids that I modify are my lc lids.


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Offlinestarbones
I'm an alien, I eat uranium.
I'm a teapot


Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 18 days
Re: Cultivation General Discussion [Re: BrownBear]
    #27045975 - 11/18/20 05:21 PM (3 years, 2 months ago)

Can you tell me the vendor or send it in PM? I would be needing around 200-300


--------------------
Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.




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OfflineBrownBear
Warrior-Traveler
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Registered: 06/05/20
Posts: 1,539
Last seen: 8 months, 7 days
Re: Cultivation General Discussion [Re: starbones]
    #27046016 - 11/18/20 05:51 PM (3 years, 2 months ago)

Check your :pm:


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Offlinerickomalley238
Stranger
Registered: 08/09/20
Posts: 414
Last seen: 6 months, 20 days
Re: Cultivation General Discussion [Re: BrownBear]
    #27046597 - 11/18/20 11:58 PM (3 years, 2 months ago)

I'm hoping you guys can tell me if I should do anything regarding my cakes. I birthed 6 BRF cakes 1 month ago. Two of them were GT, the other four were PF Classic.

The GT cakes fruited just fine, the PF cakes all literally have one pin with no signs of growth. Again this is after a month.

Fruiting conditions are fine; SGFC, 5" of perlite, 6500k light, and normal misting.

The only thing is the room is colder than normal, maybe 68-69 degrees at times.

Do you guys recommend I do anything to somehow get these cakes to grow? Or are they a lost cause after a month, despite having a pin on each cake?

Thanks.


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OfflineCadellhe
Stranger
Registered: 11/19/20
Posts: 1
Last seen: 3 years, 2 months
Re: Cultivation General Discussion [Re: rickomalley238]
    #27046608 - 11/19/20 12:12 AM (3 years, 2 months ago)

Hey there guys wanting to look and harvest some mushrooms this season coming have done alot of reading and watching videos from nz hawkes bay and woundering if any one else here is and would be keen to show me the dos and dont or just some good tips thanks.


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OfflineAtmozFear
just a shade of myself
Male

Registered: 01/25/19
Posts: 1,032
Last seen: 1 hour, 57 minutes
Re: Cultivation General Discussion [Re: starbones]
    #27046615 - 11/19/20 12:25 AM (3 years, 2 months ago)

Someone on here ran a test a few months ago and found two layers of micro pore tape scored higher than tightly packed polyfill.


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 9 hours
Trusted Cultivator
Re: Cultivation General Discussion [Re: Cadellhe]
    #27046626 - 11/19/20 12:35 AM (3 years, 2 months ago)

Quote:

Cadellhe said:
Hey there guys wanting to look and harvest some mushrooms this season coming have done alot of reading and watching videos from nz hawkes bay and woundering if any one else here is and would be keen to show me the dos and dont or just some good tips thanks.



There's tons of information in my signature as well as the signatures of many of our experienced cultivators.

Start with agar is pretty much the only tip I have energy for right now


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlinestarbones
I'm an alien, I eat uranium.
I'm a teapot


Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 18 days
Re: Cultivation General Discussion [Re: AtmozFear]
    #27046630 - 11/19/20 12:38 AM (3 years, 2 months ago)

Quote:

AtmozFear said:
Someone on here ran a test a few months ago and found two layers of micro pore tape scored higher than tightly packed polyfill.




I started with one layer so I could dial and yeah one layer is causing things to dry out faaaast as fuck. Going to double up the bottoms as per Pasty then monitor the situation from there.

Wait, what do you mean by scored higher. As a jar filter or for monotub FAE. Now I'm confused and very high.


--------------------
Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.




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OfflineAtmozFear
just a shade of myself
Male

Registered: 01/25/19
Posts: 1,032
Last seen: 1 hour, 57 minutes
Re: Cultivation General Discussion [Re: starbones]
    #27046634 - 11/19/20 12:42 AM (3 years, 2 months ago)

Lol oh I mean jar filter

They had some kind of meter gauging contams


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Edited by AtmozFear (11/19/20 12:44 AM)


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InvisibleMtbromo
The Bro


Registered: 08/27/20
Posts: 186
Re: Cultivation General Discussion [Re: AtmozFear]
    #27046638 - 11/19/20 12:46 AM (3 years, 2 months ago)

What happens to a tub if the room temperature gets above 100f for a day or so? But then goes back to 75-85f

Bad or not a problem?


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Knowledge and Healing in a Handful.


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