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OfflineFailboat
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Re: Cultivation General Discussion [Re: tedoro]
    #27038523 - 11/14/20 10:06 AM (3 years, 2 months ago)

First of all is your coir of high purity. Just cause you got a "5kg" block it doesn't mean there's 5kg of coir. I get the expensive shit and the hrow store followed suit and no longer stovks the B grade which was visbly littered with stones and sand.


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Offlinetedoro
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Re: Cultivation General Discussion [Re: Failboat]
    #27038536 - 11/14/20 10:13 AM (3 years, 2 months ago)

Quote:

Quirkmeister92 said:
First of all is your coir of high purity. Just cause you got a "5kg" block it doesn't mean there's 5kg of coir. I get the expensive shit and the hrow store followed suit and no longer stovks the B grade which was visbly littered with stones and sand.




I have found a sri lanka brand... down to earth... 12k. Its some of the cleanest I've found. been using it all summer. I weigh 650 out for each bucket.

But coir brands are beside the point. I think one can cook it halfway to mush... reducing its water-holding capacity.


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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InvisiblePastywhyteMDiscord
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Re: Cultivation General Discussion [Re: tedoro]
    #27038540 - 11/14/20 10:14 AM (3 years, 2 months ago)

I’ve pressure cooked coir for 90 min at 15 psi. It still held the water fine IIRC. Maybe some breakdown but not so much that it caused me issues.


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Offlinetedoro
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Re: Cultivation General Discussion [Re: Pastywhyte]
    #27038544 - 11/14/20 10:17 AM (3 years, 2 months ago)

Quote:

Pastywhyte said:
I’ve pressure cooked coir for 90 min at 15 psi. It still held the water fine IIRC. Maybe some breakdown but not so much that it caused me issues.




good to know. I will keep taking careful notes. Thanks for the response.


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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Offlinestarbones
I'm an alien, I eat uranium.
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Re: Cultivation General Discussion [Re: tedoro]
    #27038590 - 11/14/20 10:52 AM (3 years, 2 months ago)

Pantyhose I forgot yeah, do you just stretch it right over the tub?


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Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.




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Offlinethe.raven
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Re: Cultivation General Discussion [Re: starbones]
    #27038829 - 11/14/20 01:50 PM (3 years, 2 months ago)

Quote:

starbones said:
I need to figure out a way to seal the shitty lids on my monotubs so an errant fungus gnat doesn't get into them. Won't feel safe until I go a month without seeing one.




I use masking tape around the tub lid. I don't remove it or crack the lid until my first harvest.

Do you have houseplants? That can be a vector for gnats.

I use hpoo for my substrate and for garden compost. I pick up compost from stables very regularly, it's loaded with volunteers (coprinus) and gnats. Eventually in the summer, the gnats find their way indoors to my houseplants. Occassionally they try to access my cultivation space. I inoculate my garden and houseplants, about 2-3x per year with BT (bacillus thuringiensis) and steinernema feltiae (or some other species of beneficial nematodes). Both BT and nematodes will kill gnats in their larval, soil dwelling stage. Both are safe around children and pets. Sticky traps can be effective for controlling them in the adult airborne stage.

Preventing and controlling a gnat population is best if you hit them at all life stages. If you do have indoor plants with gnats, its good to do a soil change when you inoculate with beneficial organisms.


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Offlinestarbones
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Re: Cultivation General Discussion [Re: the.raven]
    #27038891 - 11/14/20 02:15 PM (3 years, 2 months ago)

Nah, no houseplants. They had come from around the house outside in the planters on the shady side that were super saturated I figure. Once inside they went after tubs hard because they're seriously attracted to trichoderma and fruit as seen in commercial white button farming.

I've all but killed most of them by using bacillus bacteria as a larvacide in my tubs and then switching over to invitro bags. Just see the odd one adult now and again and I've been monitoring their levels using yellow sticky traps affixed near lights, drains, sump pit and near my racks of bags. Lots of traps are empty now but it only takes one gnat to start it all up over again by getting into a tub. I told myself I'd take one month without seeing a single living one as they are an incredible spreader of pathogens, again seen in white button mushroom farming as they are attracted to trichoderma, get covered in living trich then find substrate to ovipost in thus spreading it.

If the idea is that a pair of panty hose can be stretched entirely over a tub then I'm game. They're pretty catastrophic for bulk growing but with winter here and taking away their ability to lay eggs the population SHOULD end.

Again it only takes one to explode their population again as they reach reproductive capability 4 hours after leaving the substrate. Just need confirmation that panty hose is capable of stretching over a monotub without the mesh becoming large enough for them to get through.


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Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.




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InvisibleMateja
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Re: Cultivation General Discussion [Re: starbones]
    #27039204 - 11/14/20 05:35 PM (3 years, 2 months ago)

How long do y'all run LC's on the magnetic stirrer? I mean do y'all run the stirrer 24/7 or one hour on one off or however? I seem to have killed my cultures from too much stirring (24/7) for three days :shrug: help a noob out


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Cakes inside Water Tub


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Offlinestarbones
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Re: Cultivation General Discussion [Re: Mateja]
    #27039225 - 11/14/20 05:47 PM (3 years, 2 months ago)

Low and slow from the get go, colonizes fairly rapidly.
I crank it to warp speed for five minutes before schlurping it up to break the myc blobs up.

My albino LC has been stirring for a few weeks and PEU for two. Never really had an LC die. Something else going wrong?


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Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.




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InvisibleMateja
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Re: Cultivation General Discussion [Re: starbones]
    #27039251 - 11/14/20 06:06 PM (3 years, 2 months ago)

A few weeks?
When I stirr manually a few times a day I get 'full' colonization in about 5-6 days, now I've heard it's even faster with a magnetic stirrer so I built a bunch of those but now I'm wondering how I should stirr for that really fast colonization of LC that I've heard about :shrug:


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InvisiblePastywhyteMDiscord
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Re: Cultivation General Discussion [Re: Mateja]
    #27039260 - 11/14/20 06:11 PM (3 years, 2 months ago)

I’ve often found the needle shreds the myc really good when you aspirate the syringe.


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Offlinestarbones
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Re: Cultivation General Discussion [Re: Pastywhyte]
    #27039313 - 11/14/20 06:48 PM (3 years, 2 months ago)

It's colonized in under a week I just keep it spinning to stop it from trying to form solid blobs and I figure it's an aerobic organism so the agitation from the stirring probably adds some oxygen to the water. Letting it do it's thing.

My 18g needles get clogged if I let an LC sit to long and it starts to.. solidify? Not sure what to call it. Had Cambodian LC that looked like it wanted to fruit.


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Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.




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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: starbones]
    #27039383 - 11/14/20 07:17 PM (3 years, 2 months ago)

Id call it consolidation


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineMLPismyOPSEC
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #27039453 - 11/14/20 07:44 PM (3 years, 2 months ago)

Just a heads up to anyone that needs to stock up on coir, i've always used Plantonix, and they sent a postcard with my order. 20% off your entire order on www.bagicho.com coupon code BAG20. I'm not affiliated with them in any way, and hoping i'm not breaking the rules by posting a non-sponsor link, i just want to help some folks save some cash!


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InvisibleMateja
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Re: Cultivation General Discussion [Re: starbones]
    #27039542 - 11/14/20 08:36 PM (3 years, 2 months ago)

Aight, just wanted to make sure that cultures can be spun 24/7 in the stirrer and that should be a issue :thumbup:


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InvisibleJosex
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Re: Cultivation General Discussion [Re: Mateja]
    #27039571 - 11/14/20 09:05 PM (3 years, 2 months ago)

Quote:

Mateah said:
How long do y'all run LC's on the magnetic stirrer? I mean do y'all run the stirrer 24/7 or one hour on one off or however? I seem to have killed my cultures from too much stirring (24/7) for three days :shrug: help a noob out



I don't think you've killed anything, but I may know where you got that from :lol:

Just let the LC rest a couple days to recover.

When I'm using stirrers I personally dislike stirring 24/7 from the beginning. I wait until I see the first puffs of myc (from biopsy) and then stir like half an hour a couple times a day. I increase the time as the culture grows and once there's enough mycelial mass I put it on the stirrer 24/7.


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InvisibleMateja
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Re: Cultivation General Discussion [Re: Josex]
    #27039578 - 11/14/20 09:14 PM (3 years, 2 months ago)

Okej I inoculated mine with 25ml donor LC each so that's enough for 24/7 stirring I believe but still 3 days later all I have are these tiiiiny fragments of myc that seems to equate to roughly 25ml still (only chopped up much finer) meh.. I'm sterilizing 15 new broths right now 10 will go on the stirrers and I'll try stirring slightly differently for each to see what's up.


This is the looks of a Pan Cyan LC that was inoculated with 25ml and spun for 3 days.
I'd doesn't seems to have grown and instead sinks to the bottom lookin lifeless within 5 min.


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Cakes inside Water Tub


Edited by Mateja (11/14/20 09:17 PM)


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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: Mateja]
    #27039795 - 11/15/20 01:05 AM (3 years, 2 months ago)

Document that shit yo.

That'll be a solid contribution


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisibleMateja
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #27039887 - 11/15/20 03:11 AM (3 years, 2 months ago)

I think I might have something much more exciting than figuring out how to stirr LC's, and this new project I've been working on is figuring out an antibacterial solution that will grow mycelium. So far I'm very early into testing this thing out but after the initial test I've gained lots of hope and it already looks promising :goodluck: I injected these new 'antibacterial' broths 3 days ago and so far mold in growing in all of them and the broths haven't turned turbid yet and they have all received over a ml of bacterial solution :evil: (meaning they would have turned completely turbid within a day normally)


The mold definitely looked a bit stressed out, more in the broths with higher concentrations of the acid I added (bacteria doesn't grow in acidic environments, absolutely hates acidic shit, at least the vast majority of them, (molds tho can tolerate lower PH as well as lower water activity much better than bacteria)


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OfflineeLeSDenes
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Re: Cultivation General Discussion [Re: Mateja]
    #27039953 - 11/15/20 05:29 AM (3 years, 2 months ago)

I used to have the magnetic stirrer 24/7, but because I have a tube in my jars for aspiring, the mycelium latches on it and big blobs form. For this reason, I switched to just stirring once a day and I won't switch back. I think there is nothing to gain if you leave it 24/7, you can have a fully colonised LC in 7-10 days anyway.


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