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InvisibleReverendMyc

Registered: 03/29/19
Posts: 1,508
Re: Cultivation General Discussion [Re: stareatclouds]
    #27030577 - 11/09/20 05:58 PM (3 years, 2 months ago)

Quote:

stareatclouds said:
Definitely needs a comparison between whole fruits for the same period. I'd heard grinding to powder made degradation faster because more of the fruit was exposed to open air/elements. I want them to store dried fruits for 3 months and then grind and test for comparison.




Agreed! I have a few variations that I would like to see as well.

They dried at room temperature in the dark. I sure would like to see what difference, if any, had they dried using various dehydrator temperatures and times.

Also, the powdered vs whole storage. I see it stated as fact that powdered is less stable for the reasons you gave (not saying that you did SAC). But is that speculation or has it been tested?

At least this study gives some clinical results. Hopefully with some of the recent changes in US legislation we will begin to see more studies like these.


--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
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Offlinetrippytimes
♡contamination queen♡
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Registered: 09/02/14
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Re: Cultivation General Discussion [Re: ReverendMyc]
    #27030601 - 11/09/20 06:11 PM (3 years, 2 months ago)

Well here is a update this is 4.5 tubs worth I quit for the night I have work so yeah.. I quit counting after the first two tubs this isolate is a keeper didn't know these fruits were so dense and not hollow. Gonna have the numbers by tommorow night when I get done with the 6 tubs and dryed. Have to pick up a weight watchers scale or something. All three dehydrators are full two are slam packed.


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want more info on shrooms "click"


Edited by trippytimes (11/09/20 06:12 PM)


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Offlinesmalltalk_canceled
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Re: Cultivation General Discussion [Re: trippytimes]
    #27030646 - 11/09/20 07:02 PM (3 years, 2 months ago)

Fruiting my first king oysters!


More like 'jack' sized oysters, amirite?



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Willpower is the one true virtue



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InvisibleMycolorado
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Registered: 07/23/16
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Re: Cultivation General Discussion [Re: smalltalk_canceled]
    #27030666 - 11/09/20 07:12 PM (3 years, 2 months ago)

lol


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Offlinestarbones
I'm an alien, I eat uranium.
I'm a teapot


Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 18 days
Re: Cultivation General Discussion [Re: smalltalk_canceled]
    #27030669 - 11/09/20 07:14 PM (3 years, 2 months ago)

Bacillus m. being used in commercial cultivation to stimulate primordia formation makes sense and I can guarantee other bacillius have similar effects.

Just used BTI bacillus therengeia israelis or whatever to treat monotubs to kill fungus gnat larvae and the pins and sheer amount of fruit were EXCEPTIONAL. I will repost the pics when back on PC. Zero negative effects from having it in my misting water, no deformed fruit, no bacterial blotching on caps. Now I am going to treat a few bags in my current grow to compare.


--------------------
Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.




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InvisibleLoadedFish
Psilocybin Enthusiast


Registered: 07/12/20
Posts: 107
Re: Cultivation General Discussion [Re: starbones]
    #27030704 - 11/09/20 07:48 PM (3 years, 2 months ago)

Does uneven substrate reduce your overall yields or just concentrate the same yield into a smaller area?


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Offlinetrippytimes
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Re: Cultivation General Discussion [Re: LoadedFish]
    #27030714 - 11/09/20 07:55 PM (3 years, 2 months ago)

Quote:

LoadedFish said:
Does uneven substrate reduce your overall yields or just concentrate the same yield into a smaller area?



Not really sure tbh I keep my sub as level as possible... I would thinking the lower parts would grow better then higher parts


--------------------



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Offlinestarbones
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Registered: 03/04/20
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Re: Cultivation General Discussion [Re: LoadedFish]
    #27030743 - 11/09/20 08:20 PM (3 years, 2 months ago)

Uneven subs are a cock to harvest if you're a cutter since you can't just easily glide your knife. In some parts you'll cut your sub off in others you'll leave high stumps. There's also the consideration that it would lead to a more favorable microclimate in one part of your tub than another and encourage uneven growth but I doubt it.

In the end it takes no extra effort to smooth it the best you can.

Those research papers on bacillus for trichoderma are interesting. Especially that they can control trichoderma aggressivum in commercial cultivation since the group 1 fungicide t-methyl cannot and that's why there's super strict hygiene and containment protocols when it comes to it in those operations.

I don't believe we deal with t. aggressivum in what we do. From reading I couldn't imagine being able to grow much if that shit was contaminating your home. It's called aggressivum for a reason. Would be interesting to play around with bacillus but when it comes to things like that I think it's out of the reach of a home gamer because it would require some pretty extensive labwork to isolate and cultivate it. BTI is only available to us because of its use in mosquito/gnat control.

I dunno, t-methyl is a proven option used as a casing drench for white button farming and I see no reason it shouldn't be used for slow fruiting genetics like PE and such. If you don't want to use it fine but if someone were large scale growing.. why not? Case as normal and give it a spritz from a wand sprayer. RR and Workman signed off on its effectiveness so I see no reason I shouldn't use it. Again yeah you have success without it but.. why not? I think opposition to it is dumb when farmers use it for their crops, white button farmers use it for theirs but some guy on Shroomery thinks because we grow in a Wal-Mart storage tub somehow it's a bad idea. If anything it's a better idea.

If peat casings need calcium hydroxide to stave off molds it's illogical to say that it's shameful or silly to use t-methyl on anything else. I get one is just hydrated lime and the other is a fungicide but it's still chemically treating your casing material with the end goal to be growing mold free.


--------------------
Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.




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Invisiblestareatclouds
star eat clouds?
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Registered: 09/29/14
Posts: 9,887
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Re: Cultivation General Discussion [Re: starbones]
    #27030759 - 11/09/20 08:34 PM (3 years, 2 months ago)

Quote:

Yeetusdeetus said:
Does casing with coir at spawning really do anything? When I’ve tried it the myc just colonized the coir but the pinset wasn’t really any better than a late casing




It allows me to fruit immediately without drying out my sub.


Quote:

starbones said:
Oh this was one of my Pasty tubs, actually two of em got trich which didn't show up until after a canopy was going so.. yeah that's from me misting way too much since I figure once that canopy was up around the sub it REALLY just held the water there.

Not overly aggressive the trich, just a small patch  that only affected a few fruits in a size about the circumference of a tennis ball. I let the tubs completely finish and it barely grew. Looks like to me anyways it was growing on some exposed grain. Same thing on the other unit. The patches were closer to the middle of the tub and around the area where the first biggest fruits came in.




I think it's more likely your spawn had mold to begin with. You know, since the grain in your tub is growing green? Either way, I wouldn't let sporulating mold keep growing in your grow areas. It'll just make it easier to get green in the future.

Not really sure what the rest of your post meant, tbh. I think you're overthinking things, though. Holler if you ever have a specific question about the process because that'd be easier to troubleshoot.

Post a picture of your colonizing spawn jars if you can.


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OfflineRumpleforeskin
Scientist new to mycology
Male


Registered: 07/05/20
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Re: Cultivation General Discussion [Re: ReverendMyc]
    #27030816 - 11/09/20 09:27 PM (3 years, 2 months ago)

Quote:

MuthaFudpucker said:
Quote:

stareatclouds said:
Definitely needs a comparison between whole fruits for the same period. I'd heard grinding to powder made degradation faster because more of the fruit was exposed to open air/elements. I want them to store dried fruits for 3 months and then grind and test for comparison.




Agreed! I have a few variations that I would like to see as well.

They dried at room temperature in the dark. I sure would like to see what difference, if any, had they dried using various dehydrator temperatures and times.

Also, the powdered vs whole storage. I see it stated as fact that powdered is less stable for the reasons you gave (not saying that you did SAC). But is that speculation or has it been tested?

At least this study gives some clinical results. Hopefully with some of the recent changes in US legislation we will begin to see more studies like these.





Might be worth buying a vacuum sealer to pull out the oxygen then?  Or maybe buy a tank of CO2 and pump some in?  It works for 7-11 chili dogs.....


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Offlinestarbones
I'm an alien, I eat uranium.
I'm a teapot


Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 18 days
Re: Cultivation General Discussion [Re: stareatclouds]
    #27030819 - 11/09/20 09:33 PM (3 years, 2 months ago)

The grains weren't growing green until the first flush had completed. These grains were exposed.

As per RR exposed grains are a mold magnet.

RogerRabbit said:
Never leave grains exposed on a substrate.  It leads to a much higher than normal contamination rate.
RR

He's got a ton of posts saying this. Stating that when the mycelium dies back on the exposed grain it becomes a mold magnet. I had enough FAE and moisture evaporation on these grains to get away with it and my OCD misting until the flush was in and then I didn't anymore.

I always used to cover my grains but I stopped and couldn't remember the fuck why until my memory kicked in and..

bodhisatta said:
I rarely cover my grains. Just mix and go

It's really difficult sometimes to sort through the amount of conflicting information here on Shroomery but this one really irks me because it's a presentation of logical thought from RR about leaving an exposed nutritious grain the mycelium has died back on out for mold to germinate on and then bod's indifferent instruction there.

One makes sense and the other just seems like falling upwards successfully. Why I changed from RR to bod on this I do not know.

As for my spawn it's clean. My LC have all been tested on agar now multiple times. I've been having great success growing invitro with bags using polyfil necks to keep contaminates out best I can.

I don't know why I keep changing things that worked in the first place but that's the problem I'm arriving at. Taking successful information then getting curious and reading shit from other people and changing to it thinking I was doing something incorrectly. Exposed grains is dumb, not spending the extra two minutes to spread some coir on top of the grains is dumb, lazy and bad practice/information.

200+ wet pounds in a month and I changed things because I was bored, stupid and doubting.


--------------------
Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.




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Offlinetryptkaloids
Learner
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Re: Cultivation General Discussion [Re: starbones]
    #27030874 - 11/09/20 10:40 PM (3 years, 2 months ago)

Quote:

starbones said:
The grains weren't growing green until the first flush had completed. These grains were exposed.

As per RR exposed grains are a mold magnet.

RogerRabbit said:
Never leave grains exposed on a substrate.  It leads to a much higher than normal contamination rate.
RR

He's got a ton of posts saying this. Stating that when the mycelium dies back on the exposed grain it becomes a mold magnet. I had enough FAE and moisture evaporation on these grains to get away with it and my OCD misting until the flush was in and then I didn't anymore.

I always used to cover my grains but I stopped and couldn't remember the fuck why until my memory kicked in and..

bodhisatta said:
I rarely cover my grains. Just mix and go

It's really difficult sometimes to sort through the amount of conflicting information here on Shroomery but this one really irks me because it's a presentation of logical thought from RR about leaving an exposed nutritious grain the mycelium has died back on out for mold to germinate on and then bod's indifferent instruction there.

One makes sense and the other just seems like falling upwards successfully. Why I changed from RR to bod on this I do not know.

As for my spawn it's clean. My LC have all been tested on agar now multiple times. I've been having great success growing invitro with bags using polyfil necks to keep contaminates out best I can.

I don't know why I keep changing things that worked in the first place but that's the problem I'm arriving at. Taking successful information then getting curious and reading shit from other people and changing to it thinking I was doing something incorrectly. Exposed grains is dumb, not spending the extra two minutes to spread some coir on top of the grains is dumb, lazy and bad practice/information.

200+ wet pounds in a month and I changed things because I was bored, stupid and doubting.



Id be careful quoting a dinosaur.

What is that advice dated?

I'm not convinced leaving axenic grain exposed causes mold.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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Offlinefightingcherries
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Registered: 10/23/13
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Re: Cultivation General Discussion [Re: stareatclouds]
    #27030876 - 11/09/20 10:41 PM (3 years, 2 months ago)

I have a lot of tubs going, all with 6 - 2" diameter holes.  I cover them with one layer of micropore tape.  They are 54 qt tubs with the weather seal lids.  Everything is going great, except the only thing is misting so many daily is annoying.  I was going to play around with humidifying the air in the room to hopefully mist less days per week.  I cant go too high on the humidity, because the room is not set up for that high of a level and Id probably have to take everything out and paint and seal a bunch of stuff.


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OfflineMH5109
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Re: Cultivation General Discussion [Re: fightingcherries]
    #27030881 - 11/09/20 10:45 PM (3 years, 2 months ago)

Peu t2 culture that went to grain 10/10, spawned 11/4 didn't turn out so well, it has been in the 60s and low 70s throughout. Im kinda debating on spawning the other two jars from the same line, the jar is a peu t2 a2g 10/10 in the same temp area as the tub. Mold has kinda been killing me I have been struggling to get a first flush.


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OfflineDj_karma_magnet
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Registered: 04/03/19
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Re: Cultivation General Discussion [Re: MH5109]
    #27030890 - 11/09/20 10:53 PM (3 years, 2 months ago)

How sealed do my bags have to be? I have been sealing and pressure cooking my rye bags 1 by 1 (small, electric pressure cooker) and I’ve noticed that air kind of gets out of some of them...will this cause issues or is this normal/okay?


Edit: also, got 14 bags and a couple 12cc syringes. Will .75-1cc per bag be okay? Only trying to use 1 syringe. The bags have 1.5 lb rye berries each (was 1.3 before hyrdation)
Thanks


Edited by Dj_karma_magnet (11/09/20 11:33 PM)


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OfflineRumpleforeskin
Scientist new to mycology
Male


Registered: 07/05/20
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Re: Cultivation General Discussion [Re: Dj_karma_magnet]
    #27030905 - 11/09/20 11:14 PM (3 years, 2 months ago)

Not sure if I should post here or elsewhere, but I"m looking for clarity on Penis Envy varieties.
I've read that this variety tends to have higher levels of the active ingredient. Is this true for all of them?  Is PE6 as strong as PEU?  Is PEU as strong as APE?
Thanks


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Invisiblestareatclouds
star eat clouds?
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Re: Cultivation General Discussion [Re: starbones]
    #27030931 - 11/09/20 11:57 PM (3 years, 2 months ago)

Quote:

starbones said:
The grains weren't growing green until the first flush had completed. These grains were exposed.




I read the same RR advice when I first started and vowed to never leave grains exposed. Then active growers told me they never worry abut it. So I stopped worrying about it. And nothing changed.

Your tub not showing green until your first flush completed doesn't mean anything regarding clean spawn, either. Not all dirty spawn shows itself immediately. You can absolutely have mold hitching a ride that doesn't show until your second flush. You also previously said the green showed up during the first flush earlier, too. :shrug:

Sounds like you've got your mind made up on everything, though. Hope your green issues clear up, bro.


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InvisibleFriedEggS
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Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
Re: Cultivation General Discussion [Re: stareatclouds]
    #27030972 - 11/10/20 01:08 AM (3 years, 2 months ago)

Quote:

stareatclouds said:
I read the same RR advice when I first started and vowed to never leave grains exposed. Then active growers told me they never worry abut it. So I stopped worrying about it. And nothing changed.





:whathesaid:

we learned around the same time. i also went through the same process with outdated advice and eventually realized it was pointless to worry about exposed grain. RR is great and everyone learned a lot from him. we are all grateful for what he's contributed. but with any discipline of science, 20% of what you learn will be outdated or proven incorrect in 5-20 years. it's just hard to predict which 20%.


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: smalltalk_canceled]
    #27031034 - 11/10/20 03:53 AM (3 years, 2 months ago)

No pe6 is just a normal cube. Idk about PEu but PE and ape are usually the very potent ones.


Quote:

smalltalk_canceled said:
Fruiting my first king oysters!


More like 'jack' sized oysters, amirite?






What are you growing those on?? They might need more light, I’ve only run them once but the caps got huge once I started giving them way more light than normal. I just used a quart of wbs spawned to 50/50 coir/hwfp



I’m about to get back into them not that’s its cold, I had to put those in the fridge every night to fruit them.


--------------------
LAGM2020


Edited by A.k.a (11/10/20 03:53 AM)


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InvisibleWall.E
Bacteria's Bitch
Male


Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
Re: Cultivation General Discussion [Re: A.k.a]
    #27031057 - 11/10/20 04:43 AM (3 years, 2 months ago)

What do you guys think would be the smallest or cheapest burner i could get to run a 23qt pc in my garage?

Are there any issues to running it in a cold snap? I'm looking for answers from my viking friends not you weak kids and your "zero degrees is cold"


--------------------
Life’s shit, but I’m loving it



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