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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: FriedEgg]
#27026597 - 11/07/20 11:08 AM (3 years, 2 months ago) |
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Quote:
FriedEgg said:
Quote:
bodhisatta said: They do sell media bottle gl45 caps with all sorts of different holes and barb and even some with built in gas exchange filters specifically for growing LCs in other microbiological contexts. lc has been done in media bottles since the 50s in yeast labs
you wouldn't happen to have a link to a GL45 cap with just a gas exchange filter, would you? i'm overwhelmed with all the options everywhere. i just want to do LCs in my GL45 bottle. i'm ok pouring.
https://lionsmanedenver.com/store/1000ML-LIQUID-MEDIA-EXTRACTOR-BOTTLE-LION%E2%80%99S-MANE-BRAND-p194243377
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FriedEgg



Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27026609 - 11/07/20 11:16 AM (3 years, 2 months ago) |
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that's nice but it's sold out. i was looking for a GL45 cap that has a female (or male?) luer lock inlet so you can attach a syringe filter. i don't mind opening my LC up in front of a flowhood and pouring it into a sterile substrate bag.
Edited by FriedEgg (11/07/20 11:18 AM)
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dfwerydfhg
Stranger
Registered: 05/04/20
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Re: Cultivation General Discussion [Re: FriedEgg]
#27026610 - 11/07/20 11:16 AM (3 years, 2 months ago) |
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I've made lids like that by getting a rubber bung that fits in the media bottle neck, drilling out the most of the GL45 cap and using it as a retainer for the bung. Then you can drill a couple of holes in the bung, sized slightly smaller than some metal tube (stainless drinking straw would work). You want it to be a lot of work getting the tube in, but once it's there it can stay for good. Then use soft tubing on either end of the tubes to adapt to whatever you want. Luer for a syringe filter, luer valve for outlet syringe connex. Leave the syringe connection covered with a couple layers of foil (that run like 4 inches up the tube) after sterilization and it should be good for a while unless you're fucking with it a lot.
This has been standard practice in a few labs I've been in. Those pre-made lids are nice, but usually better places to spend money unless you're doing something like therapeutics where sterility is ridiculously important.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: FriedEgg]
#27026649 - 11/07/20 11:33 AM (3 years, 2 months ago) |
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Quote:
FriedEgg said: that's nice but it's sold out. i was looking for a GL45 cap that has a female (or male?) luer lock inlet so you can attach a syringe filter. i don't mind opening my LC up in front of a flowhood and pouring it into a sterile substrate bag.
This has the luer lock adapter attached to tubing. If you don't mind pouring then why bother. What you're describing is what I linked, but with the addition of a whatman filter 
They are sold out now, but you can likely back order.
Edit*
I misread. You don't want the ability to aspirated with a syringe, you want to attach a filter. Why don't you just modify with a filter instead of doing all of that, it's mostly pointless. You can either rtv a whatman to the lid yourself, or just drill hole and rtv a sfd.
Filters are only necessary for storage though.
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The Dalcassian
Dirty Druggie



Registered: 01/04/17
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27026653 - 11/07/20 11:36 AM (3 years, 2 months ago) |
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So AKA skooled me earlier. I hadn't realised the huautla wasn't a cube. Just streaked a plate today and read that LC is a better way of nocc'ing it up as you won't really need to shake the grains.
I've never done LC and just want to keep it simple, can I stuff the lid of a bottle with polyfill and cover with tinfoil before PC. I see you guys talking about various lids and filters but I do like to keep things low tech
-------------------- Here Lies This Individual 
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Josex
#cheat_code


Registered: 11/13/15
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27026668 - 11/07/20 11:44 AM (3 years, 2 months ago) |
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Quote:
p9hu7 said: Funny that you should mention that, I've been looking for parts so that I can do something like this...or find one from a lab supply site:


This is my DIY version.
It's funny you posted that because it was only recently that I discovered those jars even existed. I made mine like 3 years ago and I didn't copy nobody. 
The first pic is my new build, just an improvement from my ealier build. It only takes working with these once to fall in love.
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starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 18 days
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There's a cube out there people call Huautla though and it gets confusing. One of Shroomerys big sponsors carries it. Just looks like a bog standard classic cube.
Same with people having that "Blue Meanies" name for that one cube var. Where real Blue Meanies are pan cyan.
-------------------- Listen, I'm steel fisted with the iron lung Heavy metal ballads out the guitar where lions run.
 
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: Josex]
#27026674 - 11/07/20 11:47 AM (3 years, 2 months ago) |
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Those look pretty cool.
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The Dalcassian
Dirty Druggie



Registered: 01/04/17
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#27026874 - 11/07/20 01:38 PM (3 years, 2 months ago) |
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Quote:
starbones said: There's a cube out there people call Huautla though and it gets confusing. One of Shroomerys big sponsors carries it. Just looks like a bog standard classic cube.
Same with people having that "Blue Meanies" name for that one cube var. Where real Blue Meanies are pan cyan.
Yep, just spoke to the guy who gave me the print. Tis a cube... My bubble is well and truly burst
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tryptkaloids
Learner



Registered: 02/08/15
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Blue meanies are psilocybe cyan not pan.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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ModularMind
M.P.F.



Registered: 02/09/10
Posts: 7,902
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Re: Cultivation General Discussion [Re: tryptkaloids]
#27026909 - 11/07/20 01:59 PM (3 years, 2 months ago) |
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Quote:
tryptkaloids said: Blue meanies are psilocybe cyan not pan.
I’ve heard the opposite.
Edit: But you’ve also got cubes being traded with the name and then there’s this
“The Blue Meanies are a fictional army of fierce though buffoonish music-hating beings and the main antagonists in the surreal 1968 Beatles cartoon film Yellow Submarine. They allegorically represent all the bad people in the world. Their visual appearance was mostly designed by Heinz Edelmann.“ -Wiki
Edited by ModularMind (11/07/20 02:06 PM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
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Re: Cultivation General Discussion [Re: ModularMind]
#27026917 - 11/07/20 02:03 PM (3 years, 2 months ago) |
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Blue meanies are pan cyans. Also confusingly some people have called cubes them and maybe has been sold as a variety of cubes before
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starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
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Re: Cultivation General Discussion [Re: bodhisatta]
#27026975 - 11/07/20 02:34 PM (3 years, 2 months ago) |
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I've never heard anyone call Psilocybe cyan Blue Meanies. Only Pan Cyans and yeah as said before vendors have sold cube called Blue Meanies same as a big shroomery vendor is selling p cubensis "Huatula" right now. It gets shit confusing.
Having said that I don't know what real Huatula are, just the cube ones.
70% of the stuff I see grown here and nearly 90% of the stuff in other communities looks like classic cubes to me.
Then there's me trying to figure out how the fuck I obtained Averys Albinos when I never in my life had the genetics and the LC I've been cultivating is supposed to be a clone from PE6. I know a mixup might have happened but here we are.
 Just a bag of mushrooms to me.
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starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
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Re: Cultivation General Discussion [Re: starbones]
#27026991 - 11/07/20 02:43 PM (3 years, 2 months ago) |
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Here's a question for you all.
I see this kind of growth on sporeless/albino/few spore dropping vars, the gnarly growth near the cap on things like PE, Melmak, some true albinos etc. What causes this and is there a benefit in fruit weight?
Is it strictly genetic or is it caused by letting the fruits go longer because you don't have to worry about spore dumping?
I know with these Albinos I'm growing now I can let them go all day long while the later ones catch up without coming back to a black tub but is there any benefit once the cap starts to curl weight wise?
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A.k.a
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Re: Cultivation General Discussion [Re: starbones]
#27026999 - 11/07/20 02:48 PM (3 years, 2 months ago) |
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I have no idea but the gnarly varieties like that seem to be stronger on average.
Now this is a totally different organism so who knows if it’s just a coincidence but when I was learning about peppers the very hottest ones are all wrinkled and gnarly too. Something about it creates surface area which is where capsaicin is stored.
Assuming psilocybin serves the same purpose as capsaicin it would make sense.
As for the weight I have no idea. The Melmak I had were solid up until the bumps started and then became super hollow.
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LAGM2020     
Edited by A.k.a (11/07/20 02:50 PM)
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tryptkaloids
Learner



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Re: Cultivation General Discussion [Re: A.k.a]
#27027178 - 11/07/20 04:39 PM (3 years, 2 months ago) |
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Quote:
A.k.a said: I have no idea but the gnarly varieties like that seem to be stronger on average.
Now this is a totally different organism so who knows if it’s just a coincidence but when I was learning about peppers the very hottest ones are all wrinkled and gnarly too. Something about it creates surface area which is where capsaicin is stored.
Assuming psilocybin serves the same purpose as capsaicin it would make sense.
As for the weight I have no idea. The Melmak I had were solid up until the bumps started and then became super hollow.
They are both pesticides.
@starbones its probably a random mutation instead of cross contamination.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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NiftySlix
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Registered: 11/03/20
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Re: Cultivation General Discussion [Re: tryptkaloids]
#27027246 - 11/07/20 05:13 PM (3 years, 2 months ago) |
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So I inoculated a few agar Petri dishes with spores awhile ago. Had a few contams, 3 are currently showing healthy growth, and then there’s this one... it showed growth much quicker than the other 3 that are currently coming along nicely, however shortly after springing to life, it’s growth seemed... off. There were these little ridges in what I assumed to be mycelial growth and it has since stopped growing at all. Anyone know what’s going on here? It’s my first time working with agar so I’m a bit lost.
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A.k.a
Stranger



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Re: Cultivation General Discussion [Re: NiftySlix] 1
#27027250 - 11/07/20 05:16 PM (3 years, 2 months ago) |
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Bacteria colony.
They do some cool shit sometimes.
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LAGM2020     
Edited by A.k.a (11/07/20 05:17 PM)
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NiftySlix
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Re: Cultivation General Discussion [Re: A.k.a]
#27027257 - 11/07/20 05:20 PM (3 years, 2 months ago) |
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Aaah, not surprised. Thank you for your quick response and help (:
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starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
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Re: Cultivation General Discussion [Re: NiftySlix]
#27027275 - 11/07/20 05:27 PM (3 years, 2 months ago) |
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How long do you let your true albinos go for? I see some tubs on here where the caps are nearly bowled. I can't imagine they're packing on a ton of weight after the caps fully open if any at all? I know I read that there is a 10-20% weight difference from veil still attached to cap fully open but I can't see there being much beyond that.
Take the mushrooms in my paintcan in my sig, to me these have gone too far but they're not albino so they get ruined ( to me) with the spore.
Thanks trypt, a random mutation in my LC master causing all subsequent LC I've made with it to give me true albinos is fantastic news. I still learn more on that my genetics guy fucked his labels up though I ain't that lucky.
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