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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: Gastronomicus]
    #27026444 - 11/07/20 09:44 AM (3 years, 2 months ago)

I sealed the cap on my media bottle post pc, when I loosened it to drop a wedge I heard the vacuum break that had formed during cool down, they definitely sucked in a big gulp of air. This was done in front of the hood so I'm hoping that it's not an issue. I don't make enough LC to know if it even matter, so does it matter?


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InvisibleMycolorado
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #27026465 - 11/07/20 09:57 AM (3 years, 2 months ago)

As long as the bottle is clean and you’re in the flow all should be good.  Might try filtered lids to avoid the issue in the future.


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: Mycolorado]
    #27026472 - 11/07/20 10:01 AM (3 years, 2 months ago)

Perhaps I will just avoid screwing the cap down tightly while it cools. I would like to avoid drilling holes in the caps of my media bottles. If the threads are good enough protection for grain jars I would imagine that  they would be sufficient for media bottles.

My gloved hand was holding the bottle as I loosened the lid, it could have sucked contamination from my hands or arms. I guess I'll find out when I test them.:shrug:


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InvisibleMycolorado
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #27026482 - 11/07/20 10:08 AM (3 years, 2 months ago)

Right on...I glossed over the media bottle part...I wouldn’t want to drill those lids either.  I was thinking jar lids.  Mine have 3 tiny holes and a nickel sized piece of sfd rtv’d over them.  Pretty simple and seem to work fine.


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: Mycolorado]
    #27026490 - 11/07/20 10:12 AM (3 years, 2 months ago)

I would definitely modify some Mason jars without a second thought.
Hopefully I didn't carelessly contaminate all of this broth. :facepalm3:




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InvisiblebodhisattaMDiscordReddit
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #27026494 - 11/07/20 10:12 AM (3 years, 2 months ago)

They do sell media bottle gl45 caps with all sorts of different holes and barb and even some with built in gas exchange filters specifically for growing LCs in other microbiological contexts. lc has been done in media bottles since the 50s in yeast labs


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: bodhisatta]
    #27026505 - 11/07/20 10:18 AM (3 years, 2 months ago)

Funny that you should mention that, I've been looking for parts so that I can do something like this...or find one from a lab supply site:



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InvisiblebodhisattaMDiscordReddit
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #27026506 - 11/07/20 10:20 AM (3 years, 2 months ago)

They exist. Although the special caps are usually pretty expensive. And usually they're from lab supply stores so ya gotta make an account to purchase


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Offlinestarbones
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #27026508 - 11/07/20 10:21 AM (3 years, 2 months ago)

When coir goes shitty and it's NOT the spawn I say it's not even the coir itself. It's the inclusions.

I believe strongly that mold spore will have an incredibly hard time germinating on the coir itself due to natural oils and acids in the fiber. I wish the research papers on it weren't locked behind paywalls but there's cool shit like people in India using extracts to treat ringworm, etc. Having said that we know the mycelia we introduce to it has no problem growing into it from the grain we introduce.

I think that's why some people have had shit lucks with brands marketed for hydroponics that DON'T state they're enriched with trich but have luck with brands used for reptile bedding. Could be just a difference in quality from one factory to another. I know that my Zoo Med stuff is a Product of Sri Lanka while my problematic Beats Peat is made in Vietnam.

Side by side after being hydrated there is so much bullshit in the Beats Peat. It's extremely fibrous, contains far more chips and other crap.
I should sieve it two samples side by side from each just to measure the inclusion rate.

Anyway what I'm trying to stress is when the spawn is clean, mold isn't germinating on the coir it's most likely germinating on an inclusion in the coir and now can easily spread onto the coir like how our cubensis mycelia can easily spread to it from a grain.

Then there's all sorts of bullshit that could be factors in different coirs like different cultivars having different levels of antimicrobials. They're all Cocos nucifera but then you've got cultivars that are far more popular in places like Sri Lanka like the King Coconut and then in Vietnam you have Ta Coconut, Strawberry Coconut etc. It's very realistic to think that there will be a difference in pith from one to another.

All apples are apples, but cultivars differ in their composition which is why a Granny Smith tastes different than a Red Delicious. Some cultivars will be susceptible to pests and disease while others will not.

Learned the hard way the same thing about oats recently. An oat is an oat but one oat meant to become flour is not the same as an oat that is meant to be feed. They won't perform the same.


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: bodhisatta]
    #27026511 - 11/07/20 10:22 AM (3 years, 2 months ago)

The bioreactor lids can run pretty high. Those pp5 connections and tubing are pretty cheap, usually. Even that stuff can be pretty expensive.

That pic was taken from this site:

https://lionsmanedenver.com/store/1000ML-LIQUID-MEDIA-EXTRACTOR-BOTTLE-LION%E2%80%99S-MANE-BRAND-p194243377


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InvisiblebodhisattaMDiscordReddit
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Re: Cultivation General Discussion [Re: starbones]
    #27026513 - 11/07/20 10:22 AM (3 years, 2 months ago)



They make propagation setups with built in stir/agitation for media bottles but they're stupid expensive hundreds of bucks for that set-up.



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InvisiblebodhisattaMDiscordReddit
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Re: Cultivation General Discussion [Re: bodhisatta]
    #27026516 - 11/07/20 10:25 AM (3 years, 2 months ago)


Better image


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: bodhisatta]
    #27026517 - 11/07/20 10:26 AM (3 years, 2 months ago)

Quote:

bodhisatta said:


They make propagation setups with built in stir/agitation for media bottles but they're stupid expensive hundreds of bucks for that set-up.






Even the simplest of bioreactor without stir capabilities are pretty up there. It's definitely worth building yourself. I like how the bioreactor lids have a stainless steel insert where the adapters are attached though.


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InvisibleFriedEggS
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Re: Cultivation General Discussion [Re: starbones]
    #27026545 - 11/07/20 10:42 AM (3 years, 2 months ago)

Quote:

starbones said:I wish the research papers on it weren't locked behind paywalls




you can use www.sci-hub.se to bypass research paper paywalls


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OfflineYeetusdeetus
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #27026550 - 11/07/20 10:43 AM (3 years, 2 months ago)

On the subject of of filters, has anybody tried those ptfe rolls? They’re .22 micron, same as sfds. Tisch Scientific has a 10inx3m roll for $55. You could just cut little pieces off and silicone onto your lid like sfds, right?


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: bodhisatta]
    #27026559 - 11/07/20 10:51 AM (3 years, 2 months ago)

I like this. It's pricey but I love it. Just add some tubing, a whatman, and a luer lock adapter with a crimp.



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Offlinestarbones
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #27026565 - 11/07/20 10:54 AM (3 years, 2 months ago)

How the fuck is that $400.


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OfflineDj_karma_magnet
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Re: Cultivation General Discussion [Re: starbones]
    #27026572 - 11/07/20 10:56 AM (3 years, 2 months ago)

I was sitting here flustered preparing my grains reading all of the different lid teks and realizing I do not have any filter discs or silicone and I realized I have a bunch of old .5 (edit: also have .2) micron filter bags I randomly have that I never used, I now have a vac seal so I can seal them too.


Can I just inject the bag after heating the needle and slap some micropore tape over it or is it more complicated than that?

Also, will the bag melt in pc? And should I use a vac seal to seal the bag, or should I use the clips?


Edited by Dj_karma_magnet (11/07/20 11:19 AM)


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: starbones]
    #27026574 - 11/07/20 10:58 AM (3 years, 2 months ago)

Quote:

starbones said:
How the fuck is that $400.




You pay for quality and peice of mind. This is laboratory equipment.

The glass is highly resistant to heat, shock, and chemicals. Borosilicate is not cheap. That peice of equipment could be used to study lung cancer.


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InvisibleFriedEggS
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Re: Cultivation General Discussion [Re: bodhisatta]
    #27026578 - 11/07/20 11:00 AM (3 years, 2 months ago)

Quote:

bodhisatta said:
They do sell media bottle gl45 caps with all sorts of different holes and barb and even some with built in gas exchange filters specifically for growing LCs in other microbiological contexts. lc has been done in media bottles since the 50s in yeast labs



you wouldn't happen to have a link to a GL45 cap with just a gas exchange filter, would you? i'm overwhelmed with all the options everywhere. i just want to do LCs in my GL45 bottle. i'm ok pouring.


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