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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: mushboy]
#24042566 - 01/26/17 10:03 PM (7 years, 3 days ago) |
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@Moab let your scalpel sit for a little longer..I usually wait like 15-20secs then cool in agar.
Someone told me too once and I stopped having that problem with it sticking and such.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Moabfighter
Tam Fighter



Registered: 12/13/15
Posts: 2,710
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Re: Cultivation General Discussion [Re: van hatton]
#24042574 - 01/26/17 10:07 PM (7 years, 3 days ago) |
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Quote:
van hatton said: @Moab let your scalpel sit for a little longer..I usually wait like 15-20secs then cool in agar.
Someone told me too once and I stopped having that problem with it sticking and such.
Heyyyyy. That's an idea. I torch for each plate. Often relatively hot when I slice. Maybe that's it. Rock on.
-------------------- KSSS And PE WBS.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Moabfighter]
#24042586 - 01/26/17 10:15 PM (7 years, 3 days ago) |
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I guess the reason is because generally when you cool a blade in agar your just quick cooling the outside. Thus the middle is still decently hot. The agar seems to almost melt and solidify.
I'm gunna take a cut tomarrow out of a Petri and see if I just let the wedge sit on my scalpel (cooling the way I used too and uncolonized) see if it adheres to it more so with waiting a second . Idk
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Cultivation General Discussion [Re: van hatton]
#24042850 - 01/27/17 12:56 AM (7 years, 3 days ago) |
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Quote:
MycoMitch666 said: So I have a question. Does this jar look contaminated to you or is it just the rye causing the weird looking stuff?

Looks contaminated to me
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Cultivation General Discussion [Re: Teemo 6T3]
#24042942 - 01/27/17 03:34 AM (7 years, 3 days ago) |
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Hey guys,
1. I have some agar plates that are Transfers of a tissue Clone plate.
Now one of them Shows a very Nice Pin. Does it make Sense to clone that pin to a new Agar plate or is it senseless since his myc already Comes from a clone tissue?
2. the Pin formed on plate 7. from this plate I did a Transfer to plate 8 before the Pin Formed. Can I be sure, that the transferred myc on plate 8 will also Show pins when spawned?
Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Dactylium
Don't touch me I'm sterile


Registered: 08/12/16
Posts: 696
Last seen: 10 months, 13 days
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Re: Cultivation General Discussion [Re: AlCapone2k]
#24042947 - 01/27/17 03:50 AM (7 years, 3 days ago) |
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I'd clone the pin
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Cultivation General Discussion [Re: Dactylium]
#24042987 - 01/27/17 05:20 AM (7 years, 3 days ago) |
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Yeah, I thought so too, but actually I was wondering if it does make sense since it's a clone myc anyway.
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: AlCapone2k]
#24043125 - 01/27/17 07:49 AM (7 years, 3 days ago) |
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Cloning a clone is w.e
But cloning a a clone then cloning again shit gets weird and old.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Cultivation General Discussion [Re: van hatton]
#24043146 - 01/27/17 08:01 AM (7 years, 3 days ago) |
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w.e?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 4 hours, 45 minutes
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Re: Cultivation General Discussion [Re: AlCapone2k]
#24043156 - 01/27/17 08:09 AM (7 years, 3 days ago) |
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whatever
--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Tiamo]
#24043157 - 01/27/17 08:10 AM (7 years, 3 days ago) |
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ty
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Cultivation General Discussion [Re: Tiamo]
#24043159 - 01/27/17 08:11 AM (7 years, 3 days ago) |
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"Cloning a clone is whatever" ... sorry I don't understand that sentence 
Does it mean it is ok, but not necessary?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: AlCapone2k]
#24043165 - 01/27/17 08:15 AM (7 years, 3 days ago) |
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Is not a big deal your good to go.
You mycelium will just be aged. You grew a tub from it then cloned it again now throwing back to jars then tubs your culture just gets old.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Cultivation General Discussion [Re: AlCapone2k]
#24043167 - 01/27/17 08:17 AM (7 years, 3 days ago) |
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Ok, thank you
The first clone tissue was taken from a mushroom of a grow kit from the netherlands. I guess they also use clone myc.
So my first clone was at least the second clone, if I clone the pin on agar it will be at least the third clone. I think this will be ok
Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 4 hours, 45 minutes
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Re: Cultivation General Discussion [Re: AlCapone2k]
#24043175 - 01/27/17 08:20 AM (7 years, 3 days ago) |
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I wish I had cloned from my grow kit. That son of a bitch put out a few 50 gram fresh shrooms and put out more than 2 ounces for a qt of grain spawn. Not bad for 30 bucks.
--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Cultivation General Discussion [Re: Tiamo]
#24043185 - 01/27/17 08:29 AM (7 years, 3 days ago) |
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yeah, you guys know how to make grow kits
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Cultivation General Discussion [Re: AlCapone2k] 1
#24043453 - 01/27/17 10:55 AM (7 years, 3 days ago) |
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Quote:
AlCapone2k said: "Cloning a clone is whatever" ... sorry I don't understand that sentence 
Does it mean it is ok, but not necessary?
That's because technically that sentence doesn't make any sense 
God bless the English language
--------------------
Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: Cultivation General Discussion [Re: ComebackKid]
#24043459 - 01/27/17 10:57 AM (7 years, 3 days ago) |
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You can clone a clone you just dont want to keep cloning every clone but i have taken agar pins off of a clone on agar
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Moabfighter
Tam Fighter



Registered: 12/13/15
Posts: 2,710
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Re: Cultivation General Discussion [Re: ComebackKid]
#24043461 - 01/27/17 10:58 AM (7 years, 3 days ago) |
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Got hammered last night and apparently made some transfers and soaked some WBS lol. This'll be fun cooking the seed.
I think either Munch or Mush swears by two hour cook times. I have had contams lately, gonna try 120 mins vs 90 that I normally do.
Wish I could find some gypsum.
-------------------- KSSS And PE WBS.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: Moabfighter]
#24043480 - 01/27/17 11:05 AM (7 years, 3 days ago) |
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24hr soak on wbs. drain 20min(dont be crazy i just use a wire pasta strainer) load jars. they might look damp. 'whatever' pc 2hrs. i like to shake when the jars are still hot.
wait until the next day to use jars. they are perfect.
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