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OfflineGrinchGrower
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Registered: 10/02/20
Posts: 682
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Re: Cultivation General Discussion [Re: Mtbromo]
    #26992041 - 10/18/20 09:20 PM (3 years, 3 months ago)

I think colonized slants in a dedicated lab fridge (no temperature swings) are probably the best way. You might still have to transfer them to new slants periodically...


Edited by GrinchGrower (10/18/20 09:21 PM)


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InvisiblePsicomb
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Registered: 01/13/18
Posts: 4,636
Loc: the womb
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Re: Cultivation General Discussion [Re: starbones]
    #26992059 - 10/18/20 09:39 PM (3 years, 3 months ago)

Quote:

starbones said:
Quick one for pastyplate users, I made some because I ran out of normal sterile disposables. I can't find the information in the pastyplate thread about how to store them after putting a sample on them. Just unused storage which is to put them in the fridge still wrapped.

Thanks mush.




Pasty plates arent very good for long term storage.. in my experience they dry out after a month or two but they do still transfer fine at that point so you can probably get away with a transfer every 2 months if you want to keep a culture. Slants are quite a bit more ideal for long term.

For long term storage via pasty plates make the plate higher nutrient so it doesnt try to pin on it as well. You can store it on a shelf without needing to do anything or if you wanna try to make it last longer than 2 months you can put it in a ziplock bag and then in the fridge, saranwrap the outside of it if you feel inclined so it doesnt dry out through the filter.

I've been using pasty plates since I started basically and I find them super effective and easy to work with.  Hope they treat you good too..:sun:


--------------------

When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something
- nick sand


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InvisibleMtbromo
The Bro

Registered: 08/27/20
Posts: 186
Re: Cultivation General Discussion [Re: starbones]
    #26992063 - 10/18/20 09:43 PM (3 years, 3 months ago)

Would the col grain jars last a few years in a fridge?


--------------------
Knowledge and Healing in a Handful.


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Offlinestarbones
I'm an alien, I eat uranium.
I'm a teapot


Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 18 days
Re: Cultivation General Discussion [Re: Mtbromo] * 1
    #26992095 - 10/18/20 10:18 PM (3 years, 3 months ago)

Quote:

Mtbromo said:
Would the col grain jars last a few years in a fridge?




As per RR.
STORING GRAINS/PETRIS - Actually, grains have been found better for mycelium storage than agar slants,
but unfortunately take way too much room in the refrigerator. However, I've used jars that have been stored
fully colonized in the refrigerator for several years. Put them in the refrigerator at full colonization, and then
when you remove from the refrigerator, allow three to five days at room temperature before spawning.


I don't bother with slants at all after reading that but there is as RR said the problem of how much space they take. Having said that with RR saying grains are better than slants nothing is stopping you from downsizing to a pint jar of grain.
Do it with WBS and everytime you need genetics from it just use sterile tweezers to put a piece of milo on agar lol. That should last you til eternity.


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InvisibleMtbromo
The Bro

Registered: 08/27/20
Posts: 186
Re: Cultivation General Discussion [Re: starbones]
    #26992116 - 10/18/20 10:40 PM (3 years, 3 months ago)

Thanks bro! So useful info. I might even try some tiny 120ml wide mouth jars with wbs


--------------------
Knowledge and Healing in a Handful.


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Offlinestarbones
I'm an alien, I eat uranium.
I'm a teapot


Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 18 days
Re: Cultivation General Discussion [Re: Mtbromo]
    #26992125 - 10/18/20 10:51 PM (3 years, 3 months ago)

No probs.
Bookmark this.
http://www.en.psilosophy.info/pdf/all_of_rrs_notes_on_mushroom_cultivation_(psilosophy.info).pdf
Anytime you're stuck like me just pull it up CTRL+F and it'll get ya where you need to be. It's not 100% current but it's a damn fine reference.


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OfflineGrinchGrower
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Re: Cultivation General Discussion [Re: starbones]
    #26992138 - 10/18/20 11:14 PM (3 years, 3 months ago)

Quote:

starbones said:
No probs.
Bookmark this.
http://www.en.psilosophy.info/pdf/all_of_rrs_notes_on_mushroom_cultivation_(psilosophy.info).pdf
Anytime you're stuck like me just pull it up CTRL+F and it'll get ya where you need to be. It's not 100% current but it's a damn fine reference.



:thanx: Just downloaded. Nice compendium!


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OfflineInthepit
Aum Mani Padme Hum
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Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 14 days, 2 hours
Re: Cultivation General Discussion [Re: starbones]
    #26992280 - 10/19/20 04:39 AM (3 years, 3 months ago)

Quote:

starbones said:
No probs.
Bookmark this.
http://www.en.psilosophy.info/pdf/all_of_rrs_notes_on_mushroom_cultivation_(psilosophy.info).pdf
Anytime you're stuck like me just pull it up CTRL+F and it'll get ya where you need to be. It's not 100% current but it's a damn fine reference.




Thank you, that's hot stuff! 
All Of RR's Notes On Mushroom Cultivation

But I don't get the CTRL+F, didn't do a/g for meeeeee...  :rofl:
Oh! Wait, wait I see it! At the bottom of the screen OMG!


Edited by Inthepit (10/19/20 04:54 AM)


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Invisiblecoversall
إِنْ شَاءَ ٱللَهُ
 Unread Journal


Registered: 06/06/20
Posts: 2,749
Loc: संसार
Re: Cultivation General Discussion [Re: Inthepit]
    #26992283 - 10/19/20 04:46 AM (3 years, 3 months ago)

Oh boy real life has given me a break and I'm going to try to grow some of the stuff I've had sat in my fridge on agar. What's the cool, hip, à la mode rye prep tek that the hot shot popular kids are using?


--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..


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InvisibleWall.E
Bacteria's Bitch
Male


Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
Re: Cultivation General Discussion [Re: coversall]
    #26992330 - 10/19/20 05:47 AM (3 years, 3 months ago)

I just use RogerRabbit's grain prep. Soak rye for 24 hr with some gypsum, boil for 10 min, dry for 30 min, load up jars, pc and baddabingbaddaboom mushroom food


--------------------
Life’s shit, but I’m loving it



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Invisiblecoversall
إِنْ شَاءَ ٱللَهُ
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Registered: 06/06/20
Posts: 2,749
Loc: संसार
Re: Cultivation General Discussion [Re: Wall.E]
    #26992390 - 10/19/20 06:59 AM (3 years, 3 months ago)

Quote:

Myc_Hunt said:
I just use RogerRabbit's grain prep. Soak rye for 24 hr with some gypsum, boil for 10 min, dry for 30 min, load up jars, pc and baddabingbaddaboom mushroom food




Cool, I've decided to give Spitball's quick tek a shot. Got the rye draining now.

For the jar lids I figured a couple of holes covered in 2-3 layers of micropore tape should be ok for GE?


--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..


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InvisibleWall.E
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Registered: 06/05/20
Posts: 2,860
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Re: Cultivation General Discussion [Re: coversall]
    #26992449 - 10/19/20 08:10 AM (3 years, 3 months ago)

That's all I do and don't seem to have any issues


--------------------
Life’s shit, but I’m loving it



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InvisibleMoabfighter
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Registered: 12/13/15
Posts: 2,710
Re: Cultivation General Discussion [Re: Wall.E]
    #26992640 - 10/19/20 10:01 AM (3 years, 3 months ago)

Anyone have a dozen SHIP jar lids they’d be down to part with for a fee? Can PayPal.


--------------------
KSSS And PE WBS.
 


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Offlinestarbones
I'm an alien, I eat uranium.
I'm a teapot


Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 18 days
Re: Cultivation General Discussion [Re: Moabfighter]
    #26992963 - 10/19/20 01:09 PM (3 years, 3 months ago)

Quote:

Moabfighter said:
Anyone have a dozen SHIP jar lids they’d be down to part with for a fee? Can PayPal.




If nobody gets you I always see stuff like that on Etsy. Sometimes the prices are stupid though.


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OfflineRumpleforeskin
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Registered: 07/05/20
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Re: Cultivation General Discussion [Re: starbones]
    #26993150 - 10/19/20 02:53 PM (3 years, 3 months ago)

Quote:

starbones said:
Quote:

Mtbromo said:
Would the col grain jars last a few years in a fridge?




As per RR.
STORING GRAINS/PETRIS - Actually, grains have been found better for mycelium storage than agar slants,
but unfortunately take way too much room in the refrigerator. However, I've used jars that have been stored
fully colonized in the refrigerator for several years. Put them in the refrigerator at full colonization, and then
when you remove from the refrigerator, allow three to five days at room temperature before spawning.


I don't bother with slants at all after reading that but there is as RR said the problem of how much space they take. Having said that with RR saying grains are better than slants nothing is stopping you from downsizing to a pint jar of grain.
Do it with WBS and everytime you need genetics from it just use sterile tweezers to put a piece of milo on agar lol. That should last you til eternity.





So...what about holes in the lids?  Would it be better to used original lids, or can I get away with a hole and filter disk?  Thinking this might be another good idea once I get something nicely isolated.  A couple of my MSS jars are growing quite well, and I"m thinking of preserving


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InvisibleTedsDead
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Registered: 01/03/17
Posts: 4,998
Trusted Cultivator
Re: Cultivation General Discussion [Re: coversall]
    #26993187 - 10/19/20 03:16 PM (3 years, 3 months ago)

Quote:

coversall said:
Oh boy real life has given me a break and I'm going to try to grow some of the stuff I've had sat in my fridge on agar. What's the cool, hip, à la mode rye prep tek that the hot shot popular kids are using?





With a propane burner dump in rye fill with water about 2-3x the depth of the rye.  Turn burner to medium high and set timer for 40-45 minutes.  At that time the water should be hot but not boiling.  Cut heat and set timer for another 45 min.  Rye should be hydrated with no burst grain.  Drain and spread out to dry while still hot.  Should be ready real quick if u spread it out while its still steaming.

Thats what I do anyways


--------------------
weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396


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Offlinestarbones
I'm an alien, I eat uranium.
I'm a teapot


Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 18 days
Re: Cultivation General Discussion [Re: Rumpleforeskin]
    #26993199 - 10/19/20 03:23 PM (3 years, 3 months ago)

Okay I don't think this deserves its own thread so I am going to be revisiting spawn bags and trying to unfuck whatever I kept fucking up.

I went and got 100kg of wheat.
I have Unicorn 0.5 micron XL bags.

With information from Pasty and company this is the plan and speak now if I'm already fucking up.

Wheat into pot, boil for ~20 minutes hard. Pull a spoonful and check to see if the middles have hydrated.
Drain into my fuckit-buckit strainer then immediately dump onto my 4x4' strainer.
Let steam until theres little to no moisture on outside of hulls.

Fill bags each with 4 full quarts.
Place aluminum foil from bags mouth to grains so the bag does not stick to itself. Tyvek not necessary (Pastytip)
No pre-seal.
Roll air out of bags and accordion so filter patch stays dry.
Place layer of rings in bottom of 23qt Presto.
Place trivet on top of rings.
Make one layer of 3 bags if they will fit. Accordian folds up.
Make second layer of 3 bags if they will fit. Accordian folds down?
Place two plates (Mine aren't heavy on their own) on top of bags.
Fill cooker with 3 quarts of water as per manual.
Wash then lightly lube my gasket ring as per manual.
Stove to Hi. Wait for constant jet of steam.
Let vent for 10 minutes.
Place weight on. Let pressure build to 15psi and fast rock start.
Roll back temperature to normal operating and let return to 15psi.
Give it three hours.
Let cool in PC overnight (Is this necessary? I'd like to do two batches)

Next.
Remove from PC, keep accordian folds closed so I assume I can just put the bags upside down on a clean counter.
Fire up my flowhood.
Showered, facemask on. arms washed with isopropyl, gloves on.
Unfold bag infront of flowhood. Let sterile air gently form a plenum and give a spritz of CLEAN LC.
Bunch top of bag, zip tie it closed.
Move to next bag.


If there's something wrong in these steps speak now or forever hold your Reese pieces because I'm giving bags another shot based on some Shroomery advice from another thread and I'd like to have a simple as a pimple repeatable success here.


--------------------
Listen, I'm steel fisted with the iron lung
Heavy metal ballads out the guitar where lions run.




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Invisiblecoversall
إِنْ شَاءَ ٱللَهُ
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Registered: 06/06/20
Posts: 2,749
Loc: संसार
Re: Cultivation General Discussion [Re: TedsDead]
    #26993225 - 10/19/20 03:38 PM (3 years, 3 months ago)

Quote:

TedsDead said:
Quote:

coversall said:
Oh boy real life has given me a break and I'm going to try to grow some of the stuff I've had sat in my fridge on agar. What's the cool, hip, à la mode rye prep tek that the hot shot popular kids are using?





With a propane burner dump in rye fill with water about 2-3x the depth of the rye.  Turn burner to medium high and set timer for 40-45 minutes.  At that time the water should be hot but not boiling.  Cut heat and set timer for another 45 min.  Rye should be hydrated with no burst grain.  Drain and spread out to dry while still hot.  Should be ready real quick if u spread it out while its still steaming.

Thats what I do anyways




Nice this is will go on the list! Thank you


--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..


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InvisibleDoctor Mario
🍄🍄🍄🍄🍄
Male Unread Journal


Registered: 08/07/20
Posts: 1,894
Loc: 🇺🇸
Trusted Cultivator
Re: Cultivation General Discussion [Re: coversall]
    #26993242 - 10/19/20 03:53 PM (3 years, 3 months ago)

I had a business trip canceled today and got to come home 5 days early. Came home to my first pins ever. I fked up somewhere though cause there's side pins everywhere.



Oh the green in the corner is the reflection of a high vis shirt. Scared me when I opened up a tub and part of the myc was green 😅


--------------------



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InvisibleMunchauzen
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Registered: 06/22/11
Posts: 14,342
Trusted Cultivator
Re: Cultivation General Discussion [Re: Doctor Mario]
    #26993250 - 10/19/20 04:01 PM (3 years, 3 months ago)

How did you store it during colonization


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