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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Cultivation General Discussion [Re: Ferather] 5
#26989729 - 10/17/20 08:31 AM (3 years, 3 months ago) |
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Quote:
Ferather said:
Quote:
Pastywhyte said: You haven’t provided jack and until you do it’s all just a bunch of hot air.
You mean like your responses? It's only you so far.... Got any counter evidence, or just more hot air as you call it?
No offense, but I answered all of your questions, with evidence where needed, and links, all I'm getting now is blah blah, boo-hoo.
I can post 100’s of pics of coir grows that didn’t mold out and hit over 150% BE. Hundreds of pics. I’ve done real time grow logs and seen massive success with the way I grow. You haven’t genuinely answered any questions, you evade, post links to papers, show us a pic of a wooden peg we all saw 100 times already. I haven’t seen a single pic of what I consider a successful looking grow.
Most of these were coir and verm. The bottles are mostly straw. Tell me again why I should be growing on enriched paper.





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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Pastywhyte]
#26989732 - 10/17/20 08:33 AM (3 years, 3 months ago) |
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And what did you learn about the substrates and fungi? Or just put together either randomly and see what happens, or by following another tek?
Also, do you have pic of wood lovers, the substrate used, how much C:N you started with, how I need to add, and the pH? I would also like information on the enzymes in your Cubensis grows, and the best pH for them.
Edited by Ferather (10/17/20 08:40 AM)
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Ferather]
#26989747 - 10/17/20 08:46 AM (3 years, 3 months ago) |
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Fun fact, one of the first animals in space was a monkey, who learned what buttons to press and when by humans.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Cultivation General Discussion [Re: Ferather]
#26989750 - 10/17/20 08:47 AM (3 years, 3 months ago) |
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Quote:
Ferather said: And what did you learn about the substrates and fungi? Or just put together either randomly and see what happens, or by following another tek?
Also, do you have pic of wood lovers, the substrate used, how much C:N you started with, how I need to add, and the pH? I would also like information on the enzymes in your Cubensis grows, and the best pH for them.

You don’t need to know any of that to grow with success. Nothing you’ve uncovered is useful to someone looking to grow with success. This is the Mushroom Cultvation board, perhaps you need to continue your efforts over at Advanced Mycology where people will be impressed with your work. No one here cares.
There are three species of wood lovers in the pics I posted above, looks like they slipped in by accident. Surprised you didn’t notice them.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Pastywhyte] 1
#26989752 - 10/17/20 08:49 AM (3 years, 3 months ago) |
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But you're quick to comment on someone who does know.... How is that fair?
I'm beginning to lose all respect your you, you're too argumentative. I post for info, not to replace anything or anyone.
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ttching8475
Spawn

Registered: 03/01/18
Posts: 739
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Re: Cultivation General Discussion [Re: Ferather]
#26989789 - 10/17/20 09:20 AM (3 years, 3 months ago) |
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Just for fun, I think my next project might have to be to take my RW culture to a t-ez LC, then go trainwreck vs wl-tek, fruited using Pods tek.
-------------------- LAGM 2.022
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LoadedFish
Psilocybin Enthusiast


Registered: 07/12/20
Posts: 107
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Thank you for the insight man. I would say they were in the middle of the SAB, and I had them raised up on a short metal rack. Hopefully everything turns out okay.
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Wall.E
Bacteria's Bitch



Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
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Re: Cultivation General Discussion [Re: LoadedFish] 2
#26989826 - 10/17/20 09:50 AM (3 years, 3 months ago) |
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I think for my next project I'm gonna put RW to enriched paper
-------------------- Life’s shit, but I’m loving it
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Rumpleforeskin
Scientist new to mycology



Registered: 07/05/20
Posts: 82
Loc: Canada
Last seen: 3 years, 1 month
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Re: Cultivation General Discussion [Re: Wall.E]
#26989886 - 10/17/20 10:40 AM (3 years, 3 months ago) |
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Quote:
Myc_Hunt said:
Quote:
A.k.a said: If you do it right you could realistically run one culture for probably the rest of your life and never use spores again.
Check out the threads on slants and master/slave plates.
But besides that I think you can transfer plate to plate 100+ times no problem. Senescence is really something you would only deal with if you were to use one culture on a very large scale.
I don't know the real up-to-date info on senescence lol. I read about it in GGMM and thought it was interesting. It's hard for me to imagine ever running a plate to a point that it's just done growing, especially with cubes and pans. I've fucked with some edibles and their mycelium doesn't seem as dense or vigorous as the other species, so maybe that's something there?
Again, I really don't know anything about it other than it's a thing rofl. That's why I was telling homie that he should be good. As far as I know one print could theoretically entertain someone for the rest of their life if they really wanted it to.
Also aka, since you mentioned it I'll ask you since you answer all of my stupid questions: Is a slant just a thick piece of agar? I've been trying to search and figure out how to make slants, but it seems like all it is is fancy tubes full of agar cooling while laying at an angle. Is that right?
Thanks man... I didn't phrase the question every well. I bought ~3 MSS of 4 different varieties. I've used up two syringes of two varieties to get things going. Direct MSS to grain jars (a gamble i know but I wanted to start something), and the same varieties onto agar to start isolating genetics. I was intending on proceeding with transfers looking for good rhizomorphic growth, while using the leftovers on the plates (if clean) for A2G into my subsequent jars. Jars will be spawned to bags, and bags to tubs. TC good genetics from the tubs back to agar and continue isolating the best and putting leftovers in the jars....
Is this process flawed? I understand that mushrooms and mycelium are not like plants.... however, its a common problem with takings cuttings of cuttings of cuttings with cannabis. Eventually the genetics get tired. I'm trying to wrap my head around the best way to avoid this.... I've read very little on slants, and have had a hard time wrapping my head around it. Regardless, I have a lot of time as things are just starting, but any tips on my methodology/reasoning would be appreciated.
Thanks.
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Lenz
Misunderestimated


Registered: 08/17/20
Posts: 692
Loc: Fungistan
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Re: Cultivation General Discussion [Re: LoadedFish]
#26989894 - 10/17/20 10:46 AM (3 years, 3 months ago) |
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Quote:
LoadedFish said: Thank you for the insight man. I would say they were in the middle of the SAB, and I had them raised up on a short metal rack. Hopefully everything turns out okay.
FWIW, I've yet to see any contams on test plates that I've let sit open in the middle of my SAB on a rack for 30 seconds to a minute. I try and do them every SAB session just to check my SAB prep and it's looking pretty good so far. I don't do anything crazy either, just load up my work, cover in ISO soaked paper towels, spray with soapy water, then get to work after about 10-15 minutes.
Hope your jars turn out okay
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Wall.E
Bacteria's Bitch



Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
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I've never worked with cannabis but like aka said, if you get a good culture going on a plate you can put it on a slant, which is apparently just agar thats cooled at angle and store it in the fridge for a long time. I know dudes on here have shit in their refrigerators. I'm just getting into this too, so I don't have anything I've been proud enough of that I'm willing to keep yet.
-------------------- Life’s shit, but I’m loving it
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MLPismyOPSEC
That One Ponyfucker


Registered: 11/13/18
Posts: 884
Loc: Equestria? Mordor? Wester...
Last seen: 11 days, 12 hours
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Re: Cultivation General Discussion [Re: Wall.E]
#26989918 - 10/17/20 11:04 AM (3 years, 3 months ago) |
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Quote:
LoadedFish said: I didn’t plan as well as I should have in the SAB when doing a2g. My pasty plate sat face up with no cover for probably around 10 seconds at multiple points throughout the process, and I remember holding some of the wedges for longer than I would’ve liked at some points. Besides that I was smooth and quick. Is it likely that I fucked my jars :/
As long as you didn't wave anything non-sterile over it, and you moved pretty slowly, you're fine. I keep two tall pill bottles in my SAB while working, i lay the lid on one and lay the petri on the other, it stays open for minutes at a time. No issues!
Quote:
A.k.a said: But besides that I think you can transfer plate to plate 100+ times no problem. Senescence is really something you would only deal with if you were to use one culture on a very large scale of grain.
Made a small addition Basically, as long as you don't G2G for multiple expansions (one quart to ten quarts, one of those quarts to ten more, etc), senesence is a non-issue. 2-3 expansions is more than fine, and hell if you're doing that much expansion, you should probably find another method that's more efficient!
Edited by MLPismyOPSEC (10/17/20 11:05 AM)
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Dracultivation
Probably Imaginary



Registered: 05/19/20
Posts: 64
Loc: Mountains. High Altitude.
Last seen: 2 years, 11 months
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Re: Cultivation General Discussion [Re: MLPismyOPSEC] 1
#26989948 - 10/17/20 11:26 AM (3 years, 3 months ago) |
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Hello there!
Any general tips for helping get a second flush going? I had a decent first flush for the shoebox, but I can't seem to coax another run from it. I've given it a 'dunk' but I'm nor sure what else I can do.
-------------------- I live in the middle of nowhere, where nothing grows.
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 6 hours, 28 minutes
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Just keep conditions good and wait is all you can do. I’ve had second flushes come in as soon as a couple days and sometimes over a week.
--------------------
LAGM2020     
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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 14 days, 4 hours
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Re: Cultivation General Discussion [Re: A.k.a]
#26990018 - 10/17/20 12:20 PM (3 years, 3 months ago) |
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Hey AKA, did you make a space for that Flowhood coming yet? 
Ok the jars with little water and less grain! Going to spawn, surprisingly...
2 shoeboxes, T3 & T5 jars, from 9/28, 3 weeks. 1 jar Aspen Chips, and 1 jar coir, each shoebox. And a topping layer of coir. 2 shoeboxes going to a 66qt tub. After reading Munch's Shoebox Method.
Aspen chips are weightless, so 2.5L, 400g coir 2 L water 150F so far, another L on the stove in case, doesn't seem like numbers are gonna get an exact "field capacity", so it's add water as needed. Added .5L, now a little over field capacity. Next check coir temp with radar gun to at least 80F. And mix Baby!
TedsDead said: Well, the thermal death temp for cubes is 104F...I usually wait until the coir is 85F.
After that there's 4 more jars closely following...still PIOPPINO, it's finally cooling down to 80F here! Maybe get some surf Tuesday...here comes surf season!
17 October 2020: PIO! noob log
Edited by Inthepit (10/17/20 12:26 PM)
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Lenz
Misunderestimated


Registered: 08/17/20
Posts: 692
Loc: Fungistan
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Re: Cultivation General Discussion [Re: A.k.a]
#26990030 - 10/17/20 12:26 PM (3 years, 3 months ago) |
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Could anyone tell me why the corners of my tubs are looking very slightly yellow and the myc is a little more fuzzy? Hard to tell in pics but there is a very slight discoloration at all 4 corners.
   
This is happening across multiple different tubs, just the corners are looking a little unhealthy but the rest of the tub looks perfect.
Close up of the center of a tub:

I have them on shelves in a 75-78 degree room, 40-50% RH usually. 1:3 spawn to sub ratio, coir/verm at field capacity. All the tubs are pasty's EZ dialed tubs with the optional hole added. No misting, just letting them ride and checking in here and there.
Is it possible that they just aren't getting enough FAE in those spots? That's what I'm leaning towards but I don't really understand how that's the case since the corners are closest to the holes. Too much moisture perhaps?
Edited by Lenz (10/17/20 12:34 PM)
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Yeetusdeetus


Registered: 11/23/19
Posts: 1,242
Last seen: 7 hours, 48 minutes
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Re: Cultivation General Discussion [Re: Lenz]
#26990064 - 10/17/20 12:56 PM (3 years, 3 months ago) |
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Couple weeks ago I was making some some mea for 8 media bottles. I usually just measure out the dry ingredients for each bottle separately like in bods tek but I was in a hurry so I just boiled it all on the stove. Figured since it wasn’t frothing yet I’d be okay to leave it unattended while I grabbed my pressure cooker from downstairs.
Probably 40 seconds down and back and I see the pot is half empty and boiling over like a motherfucker, goop everywhere. Tried to salvage it by replacing the water, tossing in 16ish grams of malt and the rest of the agar in the pouch.
Inoculated a couple plates last week with it and now they’ve all got clear growth
--------------------

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Aablmd82
Mushroom mushroom man
Registered: 04/26/20
Posts: 71
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Inocuole]
#26990119 - 10/17/20 01:48 PM (3 years, 3 months ago) |
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Alright gang, I need some input on air flow and FAE. I’ve moved my grow to an unused box shower in the bathroom (I know, I know). It’s about 4’x4’x10’ with a 6” gap between the top of the unit and the ceiling. I put magnetic strips on the curtain to adhere it to the outside of the shower entrance to stop air from getting in when people use the toilet. There’s about 4” of overlap, so I think it’s sufficient.
I’m thinking of putting a PC fan or two fan near the bottom to circulate air. I don’t have any fruits atm so the only indication of poor FAE is that the surface is growing larger water beads than normal (though idk how correct this is). My hope is that the slight negative pressure will draw air down from the top, but not through the gaps in the curtain seal.
Any thoughts on this idea? I know how contaminants work after spawning, so I’m not trying to completely eliminate them, just cut down on the amount of toilet air entering my tubs for peace of mind mostly.
-------------------- Clone -> agar -> UnBODified monotub (first attempt, left unattended a week before harvesting)
Thanks BOD 🙏
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starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 18 days
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Re: Cultivation General Discussion [Re: Aablmd82]
#26990253 - 10/17/20 03:38 PM (3 years, 3 months ago) |
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Anyone got any experience with ditching syringes for LC? When doing so much I find I have to keep filling my 50ml over and over and it feels like it's introducing unnecessary chances to contaminate my LC.
Something like a media bottle with a small spout that can withstand a PC. Fill it full of LC, a stir bar then knock it up. Let it do its thing then when I need to knock things up just squirt/pour LC out. Guess I could just say fuck it and just G2G again.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 13 hours
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Re: Cultivation General Discussion [Re: starbones]
#26990263 - 10/17/20 03:43 PM (3 years, 3 months ago) |
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You can pour lc as you would g2g. Alternatively you can buy a hobby specific pcable injector guns that tube directly into the jar
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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