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Invisibledfwerydfhg
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Registered: 05/04/20
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Re: Cultivation General Discussion [Re: Pastywhyte]
    #26988932 - 10/16/20 05:47 PM (3 years, 3 months ago)

my second grow (after a pathetic pf-tek that got me a clone and like 2 g dried lol) used spent brewing grain as spawn, to coir in shoeboxes. got two fucking canopies that I haven't replicated since (with rye spawn). i like to think the spent grain is magic and i'm just not fucking up since :smile:


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InvisibleMateja
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Re: Cultivation General Discussion [Re: dfwerydfhg]
    #26988936 - 10/16/20 05:52 PM (3 years, 3 months ago)

I've never even managed to get brewers rye to colonize, myc just acts all dumfounded on it just growing really slowly on the same spot forever.


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Cakes inside Water Tub


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Offlinesmalltalk_canceled
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Re: Cultivation General Discussion [Re: Pastywhyte]
    #26988961 - 10/16/20 06:14 PM (3 years, 3 months ago)

Quote:

Pastywhyte said:
Quote:

Ferather said:
Quote:

tryptkaloids said:
Not only that, but actual numbers and ratios of materials used would go quite a way in establishing something that can be peer reviewed



https://www.shroomery.org/forums/showflat.php/Number/24012215#24012215

Quote:

Pastywhyte said:
If he wanted it to take the shape of a relevant grow and seem super in depth he could run multiple clones or isolates across a variety of substrates in controlled settings. Then he could calculate the biological efficiency of each flush/total yield. Then he could put the results through a t test to strip out the noise and then, possibly have some data that would be useful/of interest to some of the people here.



A clone was put to WBS spawn, it was spawned to 12kg of plain paper pellets (not enriched), and spawned to 600g of paper pellets (enriched).
The 12kg of plain took ages, the growth was weak, and produced roughly the same yield as the 600g enriched version.

Ask gourmet lovers what happens when they increase the nitrogen value of wood via supplementation.

----

If you say Cubensis arent wood-lovers, and cannot decay cellulose, and dont like a high nitrogen value, I will laugh.




Well for starters your sample size is minuscule, a single clone. So because one clone performed in a certain manner that settles it? I used to have a clone that could do quite well on spent brewers grain. Does that mean all cubes love spent brewers grain? I had another clone that did best in a 66 quart mono at a 1:3 spawn ratio with a half inch top layer. Did terrible if you tried to do anything else with it. Does that mean cubes are best done in that exact manner? When I compared coir verm to a manure sub I used 3 separate clones and still worried my sample size was too small.

Obviously the sub volumes are far different in your example. Did it not occur to you that cubes do not like a massive substrate? They are not amarillia, a 20 quart volume sub is as big as I would go before I worry about yields falling off. Smaller subs naturally have a much better chance at achieving a high BE. It’s a totally skewed comparison right off the hop. You’re lacking a control that is super important, for the species. Why not try it with Pans next and make a really massive sub, could probably use it to heat your house before it killed itself.

That experiment as you’ve outlined it is totally lacking control given the hypothesis and species needs.




what i think is most interesting about reading this, is how this turned my mind onto the possibility that you could be doing genetic isolations that also interacted with how well the isolation you were doing, did on a specific type of grain. before readin this, i didnt even consider that cloning/isolation could affect the interaction with the food source


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Willpower is the one true virtue



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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: smalltalk_canceled]
    #26989003 - 10/16/20 06:51 PM (3 years, 3 months ago)

I think he’s saying that spores/myc on agar will do better on spawn of the same type of nutrients. Like I’ve seen a few people say that they had jars colonize way faster when they germinated spores on grainwater agar then dropped them to grain. Idk if it’s true but the theory is that genetics that best utilize the nutrients will take off first and do better more often.

So in that line of thinking most of us are breeding cultures that are best able to utilize malt extract since that’s what’s in our plates and if we could somehow use lme as spawn they would do better than on grain.


At least I think that’s the gist of it, I ate too much weed candy and have no idea if that made sense.


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LAGM2020


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Offlinesmalltalk_canceled
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Re: Cultivation General Discussion [Re: A.k.a]
    #26989011 - 10/16/20 06:56 PM (3 years, 3 months ago)

well im a newb and always looking to learn, but after 1000 transfers you notice that the mycelium grows different on different nutrition

to me it makes sense that a isolation we do, could prefer colonizing some food souces, over others. we kinda know that isolations prefer something to be generally true,
we just dont know the depth of it.


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Willpower is the one true virtue



Edited by smalltalk_canceled (10/16/20 06:57 PM)


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OfflineIrishmule169
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Re: Cultivation General Discussion [Re: smalltalk_canceled]
    #26989023 - 10/16/20 07:03 PM (3 years, 3 months ago)



Fk I almost jacked my whole operation up.. I started working nights on Monday and the mycelium wasn’t getting the proper love all week. The Co2 level was high to say the least in my area !!
Think I’m still good.. any advice would be appreciated!!! I got more pics


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Offlinesmalltalk_canceled
Babnik
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Re: Cultivation General Discussion [Re: Irishmule169]
    #26989034 - 10/16/20 07:13 PM (3 years, 3 months ago)

Quote:

Irishmule169 said:


Fk I almost jacked my whole operation up.. I started working nights on Monday and the mycelium wasn’t getting the proper love all week. The Co2 level was high to say the least in my area !!
Think I’m still good.. any advice would be appreciated!!! I got more pics




ya tub is fruiting boy what the problem


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Willpower is the one true virtue



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OfflineYeetusdeetus
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Re: Cultivation General Discussion [Re: Irishmule169]
    #26989036 - 10/16/20 07:15 PM (3 years, 3 months ago)

Don’t different grains require different enzymes to digest? I assumed that’s why grainwater was touted for its recovery speed, since you’re already selecting for myc that produces enzymes for the grain you’re using. I could be wrong but iirc ME uses a blend of different grains


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OfflineIrishmule169
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Re: Cultivation General Discussion [Re: smalltalk_canceled]
    #26989055 - 10/16/20 07:36 PM (3 years, 3 months ago)

Quote:

smalltalk_canceled said:
Quote:

Irishmule169 said:


Fk I almost jacked my whole operation up.. I started working nights on Monday and the mycelium wasn’t getting the proper love all week. The Co2 level was high to say the least in my area !!
Think I’m still good.. any advice would be appreciated!!! I got more pics




ya tub is fruiting boy what the problem




All blobbed up in the one tub.. it wasn’t looking as pretty as usual..and I did a tea dose about 4 pm with the new shit .. lmao .got me freaked
The PESA is the real deal sheeeesh ...


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Edited by Irishmule169 (10/16/20 07:37 PM)


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InvisibleMateja
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Re: Cultivation General Discussion [Re: Yeetusdeetus]
    #26989059 - 10/16/20 07:37 PM (3 years, 3 months ago)

Quote:

Yeetusdeetus said:
Don’t different grains require different enzymes to digest? I assumed that’s why grainwater was touted for its recovery speed, since you’re already selecting for myc that produces enzymes for the grain you’re using. I could be wrong but iirc ME uses a blend of different grains




Even if there was a difference in recovery and colonization in terms of different enzymes and transition from/to similar to/from different kinds of media/substrate, maybe the difference simply isn't dramatic enough to even be noticeable by us? Idk I'm just trying to say it could prove hard to verify this even if it indeed is the case. I just found yesterday the 4 last sleeves of plates I've been looking for this week, and I've been planning on making GWA and GWLC from rye this whole week and now I will stfu about it until I have pics to post.


--------------------
Cakes inside Water Tub


Edited by Mateja (10/16/20 07:37 PM)


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InvisibleLoadedFish
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Registered: 07/12/20
Posts: 107
Re: Cultivation General Discussion [Re: Inocuole]
    #26989373 - 10/17/20 12:30 AM (3 years, 3 months ago)

I didn’t plan as well as I should have in the SAB when doing a2g. My pasty plate sat face up with no cover for probably around 10 seconds at multiple points throughout the process, and I remember holding some of the wedges for longer than I would’ve liked at some points. Besides that I was smooth and quick. Is it likely that I fucked my jars :/


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OfflineRumpleforeskin
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Re: Cultivation General Discussion [Re: starbones]
    #26989383 - 10/17/20 12:43 AM (3 years, 3 months ago)

I can confirm that there are spore vendors in Canada with this variety.  Sorry I responded to yours and not the original, but I can't find the original.


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OfflineRumpleforeskin
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Re: Cultivation General Discussion [Re: Rumpleforeskin]
    #26989403 - 10/17/20 01:04 AM (3 years, 3 months ago)

Quick beginner question - Starting with MSS on agar (and some into spawn jars).  If I move towards selecting isolations  from the MSS to agar plate and then transfer to new plates, and then begin taking tissue samples from successful fruits, when do I require additional spores (likely prints taken from the same genetics)?  In what propagation types, does the mycelium and its offspring become tired and in need of replacement?
In other words, once i get to the point of regularly taking tissue cultures (also curious at what frequency this would need to be done), when do I need to replace with new spores?  Do I ever?


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InvisibleWall.E
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Re: Cultivation General Discussion [Re: Rumpleforeskin]
    #26989508 - 10/17/20 03:02 AM (3 years, 3 months ago)

Quote:

Rumpleforeskin said:
Quick beginner question - Starting with MSS on agar (and some into spawn jars).  If I move towards selecting isolations  from the MSS to agar plate and then transfer to new plates, and then begin taking tissue samples from successful fruits, when do I require additional spores (likely prints taken from the same genetics)?  In what propagation types, does the mycelium and its offspring become tired and in need of replacement?
In other words, once i get to the point of regularly taking tissue cultures (also curious at what frequency this would need to be done), when do I need to replace with new spores?  Do I ever?




I'm not 100& solid on senescence but to my understanding you're gonna be good for a while. If you grow out the jars, then you can put clean cultures to grain later. So there's 2 grows that will produce clones or spores, so you can keep going with that route for a bit and then decide when you want to try something else.

Some others around here understand senescence a lot better than I do, but I think you're pretty much solid. If you get 10 prints from 1 grow thats enough to keep any hobbyist busy, but my problem along with a lot of other people here is that the one grow or species isn't enough. I was sourcing more spores the second I spawned my first grow because I was having so much fun with it.


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Life’s shit, but I’m loving it



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OfflineThe Dalcassian
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Re: Cultivation General Discussion [Re: LoadedFish]
    #26989522 - 10/17/20 03:36 AM (3 years, 3 months ago)

Quote:

LoadedFish said:
I didn’t plan as well as I should have in the SAB when doing a2g. My pasty plate sat face up with no cover for probably around 10 seconds at multiple points throughout the process, and I remember holding some of the wedges for longer than I would’ve liked at some points. Besides that I was smooth and quick. Is it likely that I fucked my jars :/




Depends on your sterile technique. I often leave my transfer plate open for a spell, but I always put it to the back on something raised.
Same with transfer samples, no problems holding them on the tip of my scalpel for a bit but I hold it to the back and high up away from the arm holes and so nothing can really fall on it.
I find if I try to do to much at once I get clumsy and my contam rate goes up.
If air is still and your movement fluid then you can be confident to leave to your plates open for a bit


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Here Lies This Individual





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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: The Dalcassian]
    #26989533 - 10/17/20 04:08 AM (3 years, 3 months ago)

If you do it right you could realistically run one culture for probably the rest of your life and never use spores again.

Check out the threads on slants and master/slave plates.



But besides that I think you can transfer plate to plate 100+ times no problem. Senescence is really something you would only deal with if you were to use one culture on a very large scale.


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LAGM2020


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InvisibleWall.E
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Re: Cultivation General Discussion [Re: A.k.a]
    #26989556 - 10/17/20 04:33 AM (3 years, 3 months ago)

Quote:

A.k.a said:
If you do it right you could realistically run one culture for probably the rest of your life and never use spores again.

Check out the threads on slants and master/slave plates.



But besides that I think you can transfer plate to plate 100+ times no problem. Senescence is really something you would only deal with if you were to use one culture on a very large scale.




I don't know the real up-to-date info on senescence lol. I read about it in GGMM and thought it was interesting. It's hard for me to imagine ever running a plate to a point that it's just done growing, especially with cubes and pans. I've fucked with some edibles and their mycelium doesn't seem as dense or vigorous as the other species, so maybe that's something there?

Again, I really don't know anything about it other than it's a thing rofl. That's why I was telling homie that he should be good. As far as I know one print could theoretically entertain someone for the rest of their life if they really wanted it to.

Also aka, since you mentioned it I'll ask you since you answer all of my stupid questions: Is a slant just a thick piece of agar? I've been trying to search and figure out how to make slants, but it seems like all it is is fancy tubes full of agar cooling while laying at an angle. Is that right?


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Life’s shit, but I’m loving it



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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: Wall.E]
    #26989574 - 10/17/20 04:49 AM (3 years, 3 months ago)

Yeah sometimes with a piece of wood inside it. It’s really just like a plate designed for long term storage instead of culture work.


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LAGM2020


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InvisibleWall.E
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Re: Cultivation General Discussion [Re: A.k.a]
    #26989576 - 10/17/20 04:52 AM (3 years, 3 months ago)

Thanks


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Life’s shit, but I’m loving it



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OfflineFerather
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Re: Cultivation General Discussion [Re: Pastywhyte]
    #26989719 - 10/17/20 08:17 AM (3 years, 3 months ago)

Quote:

Pastywhyte said:
You haven’t provided jack and until you do it’s all just a bunch of hot air.



You mean like your responses? It's only you so far.... Got any counter evidence, or just more hot air as you call it?

No offense, but I answered all of your questions, with evidence where needed, and links, all I'm getting now is blah blah, boo-hoo.


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


Edited by Ferather (10/17/20 08:26 AM)


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