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Becky G. Spot
Good Necky Becky

Registered: 10/12/20
Posts: 111
Loc: The Sunny Side
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Quote:
Gastronomicus said:
Quote:
Darkshadow said: Shook these pint jars on 10/8, how much longer should I wait to put these to a bulk substrate? Thanks for any recommendations.

They look ready to me but I don't use oats
Middle picture, jar on the right? Looks ready?
-------------------- - Becky G. Ditch the double standards boys. Girls like to have fun as much as you do.
Sisters if you find yourself hating, read my bio.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: BrownBear]
#26983046 - 10/13/20 09:37 AM (3 years, 3 months ago) |
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I will grow a bit then stall, the wedge would need quite a high ratio of nutrients to work as you want (more like grain).
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BrownBear
Warrior-Traveler


Registered: 06/05/20
Posts: 1,539
Last seen: 8 months, 7 days
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Re: Cultivation General Discussion [Re: Ferather]
#26983050 - 10/13/20 09:41 AM (3 years, 3 months ago) |
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Quote:
Ferather said: I will grow a bit then stall, the wedge would need quite a high ratio of nutrients to work as you want (more like grain).
But would that bit be enough to get a clean transfer?
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: BrownBear]
#26983055 - 10/13/20 09:44 AM (3 years, 3 months ago) |
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It could if you placed clean wedges around the infected one, if placed far enough. The no nutrient agar will be a bridge, in this case. You will need to play around slightly, but I believe that could work. And you wont need much extra to do it.
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BrownBear
Warrior-Traveler


Registered: 06/05/20
Posts: 1,539
Last seen: 8 months, 7 days
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Re: Cultivation General Discussion [Re: Ferather] 1
#26983060 - 10/13/20 09:46 AM (3 years, 3 months ago) |
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Thank you. You have been very helpful. I appreciate it.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: BrownBear]
#26983067 - 10/13/20 09:52 AM (3 years, 3 months ago) |
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No problem. I've done similar but with wood pegs. Record your recipes (so we know how much ME), and distancing.
Good luck. +5.
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: BrownBear]
#26983096 - 10/13/20 10:11 AM (3 years, 3 months ago) |
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Quote:
Iambrownbear said: Bacteria cannot colonize a substrate without nutrients while fungi can
That's kind of a sweeping statement considering the huge variance in growth abilities for different microbes. I'm no expert on this but I think the motility of even the most motile bacteria isn't all that effective on solid agar at all, that's why labs use motility agar of up to 0.3% to cultivate these. Without a sufficient water activity in a media you can't really get any kind of prolific bacterial growth unless you're dealing with some osmophilic and very motile types which are very rare. My hunch about this is that nutrients will play the least of rolls when it comes to making a selective growth medium. Gl
-------------------- Cakes inside Water Tub
Edited by Mateja (10/13/20 10:12 AM)
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BrownBear
Warrior-Traveler


Registered: 06/05/20
Posts: 1,539
Last seen: 8 months, 7 days
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Re: Cultivation General Discussion [Re: Mateja]
#26983112 - 10/13/20 10:18 AM (3 years, 3 months ago) |
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Quote:
Mateah said:
Quote:
Iambrownbear said: Bacteria cannot colonize a substrate without nutrients while fungi can
That's kind of a sweeping statement considering the huge variance in growth abilities for different microbes. I'm no expert on this but I think the motility of even the most motile bacteria isn't all that effective on solid agar at all, that's why labs use motility agar of up to 0.3% to cultivate these. Without a sufficient water activity in a media you can't really get any kind of prolific bacterial growth unless you're dealing with some osmophilic and very motile types which are very rare. My hunch about this is that nutrients will play the least of rolls when it comes to making a selective growth medium. Gl
You're right. I shouldn't have spoken in such absolutes. I apologise. I do agree that water being more important than nutruents in the fact that nutrients cannot be absorbed without water. Also depending on the water source could already have some form of nutients in it.
Edit: I understand now. Just looked up motility and how it relates to bacteria. Makes a lot of sense. Thank you.
Edited by BrownBear (10/13/20 10:28 AM)
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Moabfighter
Tam Fighter



Registered: 12/13/15
Posts: 2,710
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Re: Cultivation General Discussion [Re: BrownBear]
#26983292 - 10/13/20 12:17 PM (3 years, 3 months ago) |
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Can you sterilize in an instant pot?
-------------------- KSSS And PE WBS.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Quote:
Becky G. Spot said:
Quote:
Gastronomicus said:
Quote:
Darkshadow said: Shook these pint jars on 10/8, how much longer should I wait to put these to a bulk substrate? Thanks for any recommendations.

They look ready to me but I don't use oats
Middle picture, jar on the right? Looks ready?
Yes. It's not thickly colonized like the other bits but it IS colonized so it can be spawned now.
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 6 hours, 53 minutes
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Re: Cultivation General Discussion [Re: Moabfighter]
#26983298 - 10/13/20 12:20 PM (3 years, 3 months ago) |
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Yes in some of them, some seem to only do 11-12psi though. I had to read through my manual but the chicken setting is 15psi so if you’re not sure that’s what I’d go with. I also usually do 90min to two hours just in case.
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LAGM2020     
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 13 hours
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Re: Cultivation General Discussion [Re: A.k.a]
#26983321 - 10/13/20 12:40 PM (3 years, 3 months ago) |
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Quote:
Ferather said: All these years of posts, and I am yet to be corrected (proven wrong), only ever argued against.
ever consider that its because you beat around the bush talking about phenols and lignin, paper and starch? I always find it hard to understand what you're trying to teach, perhaps if you lined out your processes we would actually be able to understand what you are trying to improve in the hobby.
Quote:
Ferather said: No, the fungi has no carbon source (cannot utilize, decay agar), and probably there is no notable nitrogen, potassium in the agar either. It would be a thing by now otherwise. Due to the above reason, you add soluble nutrients to the agar as the complete food source (carbon, nitrogen, so on).
You are also speaking in absolutes... Brown bear is right, myc can colonize a non nutritious source provided it has the energy to do so. I find it hard to believe that transferring to a non nutritious plate isn't a sound method to outrun bacteria
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Lenz
Misunderestimated


Registered: 08/17/20
Posts: 692
Loc: Fungistan
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Re: Cultivation General Discussion [Re: tryptkaloids]
#26983422 - 10/13/20 01:52 PM (3 years, 3 months ago) |
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Any recommendations on an exotic to try after a couple of cube grows?
I'm gonna try and get my hands on some PE/APE spores and then if that goes well I want to potentially try growing some pans but I'm curious about people's experiences with the different exotics out there, wondering if there's some that are a bit easier than others or whatnot. Cubes have a bit too much of a body load and nausea/general uncomfortability for me and I'm really curious how the exotics differ in that regard as well.
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fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,270
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Re: Cultivation General Discussion [Re: Lenz]
#26983435 - 10/13/20 01:57 PM (3 years, 3 months ago) |
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Search “just as easy as cubes” and check out member Stonesun’s posts
Faht
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footpath
ὕδωρχοίρος

Registered: 07/16/19
Posts: 1,367
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Lenz]
#26983443 - 10/13/20 02:01 PM (3 years, 3 months ago) |
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People have put so much effort into detailing how to grow pans, I would say that they're probably a great way to expand your skillset. You'll learn more about meeting the higher demands of substrate and environment parameters that could easily be translated to the rest of your fungicultural hobby.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: footpath]
#26983450 - 10/13/20 02:06 PM (3 years, 3 months ago) |
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If you want an easier exotic p tamps are really straight foward.
Treated just like cubes
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Doctor Mario
🍄🍄🍄🍄🍄



Registered: 08/07/20
Posts: 1,894
Loc: 🇺🇸
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Re: Cultivation General Discussion [Re: mushboy]
#26983457 - 10/13/20 02:12 PM (3 years, 3 months ago) |
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All right... this is fuckin awesome! D3, you are a bad ass!

And the second coolest looking thing I came home to:
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Moabfighter
Tam Fighter



Registered: 12/13/15
Posts: 2,710
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Re: Cultivation General Discussion [Re: mushboy]
#26983459 - 10/13/20 02:15 PM (3 years, 3 months ago) |
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Quote:
mushboy said:

If you want an easier exotic p tamps are really straight foward.
Treated just like cubes

Really? Neat
-------------------- KSSS And PE WBS.
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 6 hours, 53 minutes
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Re: Cultivation General Discussion [Re: Moabfighter]
#26983466 - 10/13/20 02:22 PM (3 years, 3 months ago) |
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I second Tamps. Literally just like cubes only they like to be wetter plus the trip seems to be way nicer.
Pans are cool too but if it gets even a little too dry they usually abort.
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LAGM2020     
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Becky G. Spot
Good Necky Becky

Registered: 10/12/20
Posts: 111
Loc: The Sunny Side
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Re: Cultivation General Discussion [Re: Inocuole]
#26983469 - 10/13/20 02:23 PM (3 years, 3 months ago) |
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Quote:
Inocuole said:
Quote:
Becky G. Spot said:
Quote:
Gastronomicus said:
Quote:
Darkshadow said: Shook these pint jars on 10/8, how much longer should I wait to put these to a bulk substrate? Thanks for any recommendations.

They look ready to me but I don't use oats
Middle picture, jar on the right? Looks ready?
Yes. It's not thickly colonized like the other bits but it IS colonized so it can be spawned now.
Okie doke, go with that for sure

(I'm the little black kid in that one.) XOXOX
-------------------- - Becky G. Ditch the double standards boys. Girls like to have fun as much as you do.
Sisters if you find yourself hating, read my bio.
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