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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 2 hours, 48 minutes
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Re: Cultivation General Discussion [Re: Yeetusdeetus]
#26970984 - 10/05/20 05:40 PM (3 years, 3 months ago) |
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Somebody posted a grow a while back where he had cloned a clone four times and it grew normally.
There’s for sure tons of strains in a clone. For example right now I’m working on this one which has crazy sectoring still even on t4.
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LAGM2020     
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starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 18 days
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Re: Cultivation General Discussion [Re: A.k.a]
#26971028 - 10/05/20 06:03 PM (3 years, 3 months ago) |
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Right on. I assume though that because I've narrowed the genetics down quite a bit more than say MS that finding something that drops minimal spore or something albino would be exceptionally rare? I mean instead of a million different phenotype possibilities I may have narrowed it down to a few thousand?
I love everything about what I'm working with now and how I lucked out by having something that looks unique given the parent but I've gone back to square one elsewhere seeking something that truly offers what I'm looking for with minimal spore drop, yield, looks great when dry and is a good deal potent. I've started many, many a shoebox and tub strictly for the purpose of clone-hunting from the seemingly endless supply of spore syringes I had accumulated.
I'm also hoping that within the LC of the PE6 I received from a friend that it's 1) Actually PE6 and 2) Has at least one or two fruits that express the PE phenotype. Hard to get a PE/Melmak syringe domestically within Canada as I do not want to deal with customs.
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Dracultivation
Probably Imaginary



Registered: 05/19/20
Posts: 64
Loc: Mountains. High Altitude.
Last seen: 2 years, 11 months
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Re: Cultivation General Discussion [Re: A.k.a]
#26971038 - 10/05/20 06:09 PM (3 years, 3 months ago) |
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If you (the hive mind) had to rank the causes from most likely to least likely, what do you think would be the highest risk for using grain spawn and getting contams?
I'm guessing so long as the grain has been been PC'd for an appropriate amount of time after loading (I PC mine for two hours generally)it would be the inoculation?
Or are improper filters a common problem? (I use the polyfil method in 1/4" holes)I notice my quart jars almost always stall before they are fully covered.
Or is growth most susceptible to contams when mixing with casing in shoeboxes?
Trying to narrow down some different TEKs I should work on perfecting.
-------------------- I live in the middle of nowhere, where nothing grows.
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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if you are using polyfil in a 1/4" hole, it should be packed about as tightly as you can possibly make it. or ya the top tends to dry out. loose packed poly can let too much fae in.
there is no risk for contams when you mix it with substrate to fruit.
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RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,738
Loc: State of Confusion
Last seen: 3 hours, 44 minutes
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Quote:
Dracultivation said: If you (the hive mind) had to rank the causes from most likely to least likely, what do you think would be the highest risk for using grain spawn and getting contams?
I'm guessing so long as the grain has been been PC'd for an appropriate amount of time after loading (I PC mine for two hours generally)it would be the inoculation?
Or are improper filters a common problem? (I use the polyfil method in 1/4" holes)I notice my quart jars almost always stall before they are fully covered.
Or is growth most susceptible to contams when mixing with casing in shoeboxes?
Trying to narrow down some different TEKs I should work on perfecting.
In my experience, almost all of my contams come down to improper grain prep. Once I clean a culture on agar, I rarely have problems. I used to have a hell of a time prepping my grains, usually too wet, and at that time I had a lot of contamination issues. Once I learned to properly hydrate my grain of choice, everything sort of fell into place.
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Dracultivation
Probably Imaginary



Registered: 05/19/20
Posts: 64
Loc: Mountains. High Altitude.
Last seen: 2 years, 11 months
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Quote:
RoscoeReturns said:
Quote:
Dracultivation said: If you (the hive mind) had to rank the causes from most likely to least likely, what do you think would be the highest risk for using grain spawn and getting contams?
I'm guessing so long as the grain has been been PC'd for an appropriate amount of time after loading (I PC mine for two hours generally)it would be the inoculation?
Or are improper filters a common problem? (I use the polyfil method in 1/4" holes)I notice my quart jars almost always stall before they are fully covered.
Or is growth most susceptible to contams when mixing with casing in shoeboxes?
Trying to narrow down some different TEKs I should work on perfecting.
In my experience, almost all of my contams come down to improper grain prep. Once I clean a culture on agar, I rarely have problems. I used to have a hell of a time prepping my grains, usually too wet, and at that time I had a lot of contamination issues. Once I learned to properly hydrate my grain of choice, everything sort of fell into place.
I am currently using raw oat horse feed, boiled for 2 to 2.5 hours. I then let it air dry for a few hours, but after I PC them in the jars there is absolutely still residual moisture. I take it I shouldn't have that then?
-------------------- I live in the middle of nowhere, where nothing grows.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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You boil oats for 2 hours?
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Dracultivation
Probably Imaginary



Registered: 05/19/20
Posts: 64
Loc: Mountains. High Altitude.
Last seen: 2 years, 11 months
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Re: Cultivation General Discussion [Re: mushboy]
#26971130 - 10/05/20 07:08 PM (3 years, 3 months ago) |
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Quote:
mushboy said: You boil oats for 2 hours?
I suppose boil is the wrong word. I simmer them on low heat for this period of time. I never really get my large cooking pot I put them to a boil to avoid popping the grain.
-------------------- I live in the middle of nowhere, where nothing grows.
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Yeetusdeetus


Registered: 11/23/19
Posts: 1,242
Last seen: 4 hours, 8 minutes
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Sometimes there’s still moisture in my rye jars right after pc, but after a shake and letting them sit for a day it clears up. Don’t know if it’s similar for oats
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Doctor Mario
🍄🍄🍄🍄🍄



Registered: 08/07/20
Posts: 1,894
Loc: 🇺🇸
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I've been following Eat's no soak tek. Boil enough water to double the height of the oats, when water comes to a rolling boil remove the pot from heat, dump in oats, cover lid, let soak for 45 minutes and strain the oats.
He lets his oats strain for 10 minutes before putting into jars. I waited an hour just because I got busy and wanted to let them dry more anyway. There was a lot of condensation in the jars after the PC cycle. I just rolled the jar once a day for 2 days until the oats absorbed the rest of the water.
The second time I did oats, I let them dry over night (about 11 hours). There was still condensation in the jars after PC but both ways produced good jars. 2 hours is probably too long.
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Dracultivation
Probably Imaginary



Registered: 05/19/20
Posts: 64
Loc: Mountains. High Altitude.
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Re: Cultivation General Discussion [Re: Doctor Mario]
#26971170 - 10/05/20 07:42 PM (3 years, 3 months ago) |
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I appreciate the insight folks!
When I start in on my next batch of grain (in around a week or two) I'll be sure to try out lower soaking times and update my progress in my WIP.
As it stands I need to get some more agar going and hope I don't run out of spores to study. I don't trust any of my current remaining jars for g2g transfers. I'm getting pretty paranoid about the possibility of hiding contams.
-------------------- I live in the middle of nowhere, where nothing grows.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Quote:
Dracultivation said:
Quote:
mushboy said: You boil oats for 2 hours?
I suppose boil is the wrong word. I simmer them on low heat for this period of time. I never really get my large cooking pot I put them to a boil to avoid popping the grain.
That's still really really long got any pics?
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Dracultivation
Probably Imaginary



Registered: 05/19/20
Posts: 64
Loc: Mountains. High Altitude.
Last seen: 2 years, 11 months
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Re: Cultivation General Discussion [Re: mushboy]
#26971175 - 10/05/20 07:45 PM (3 years, 3 months ago) |
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Quote:
mushboy said:
Quote:
Dracultivation said:
Quote:
mushboy said: You boil oats for 2 hours?
I suppose boil is the wrong word. I simmer them on low heat for this period of time. I never really get my large cooking pot I put them to a boil to avoid popping the grain.
That's still really really long got any pics?
Only of a few recently cooked jars. I'll need to go take/upload some photos.
I don't know if this is applicable information, but my jars almost always stall at around 60 - 80% colonization.
*Off to take photos*
-------------------- I live in the middle of nowhere, where nothing grows.
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Dracultivation
Probably Imaginary



Registered: 05/19/20
Posts: 64
Loc: Mountains. High Altitude.
Last seen: 2 years, 11 months
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The promised photos of two different runs of jars. It's probably the least amount of 'wetness' I've ever had in my grain to be honest.

-------------------- I live in the middle of nowhere, where nothing grows.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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they need to be drier. Like dry dry before you pc.
With 2hr simmer my oats would all burst. Yours look ok.
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smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,862
Last seen: 13 days, 7 hours
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anyone ever had a blow fly infestation? blow or "puke" fly in some countries.
Calliphoridae and Rhiniidae
I see one hanging around my grow and it gives me the creeps
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starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 18 days
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Quote:
smalltalk_canceled said: anyone ever had a blow fly infestation? blow or "puke" fly in some countries.
Calliphoridae and Rhiniidae
I see one hanging around my grow and it gives me the creeps
Those Calliphoridae look like the common housefly here which I guess is because there's 1,200 species of Callis.. I don't get them indoors but I get them outdoors and they're a right pain in the ass because I can't swat them they're too fast. I hate all insects in general though but I especially find flies that puke on their food to eat it especially disgusting. That and when they rub their little hands together like they've got some grand scheme in the works.
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Ovoidhunter
Buttery Crescent



Registered: 09/17/16
Posts: 2,016
Last seen: 2 years, 8 months
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Re: Cultivation General Discussion [Re: starbones]
#26971237 - 10/05/20 08:14 PM (3 years, 3 months ago) |
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Is honey better for making LC's than karo or corn syrup? And if so why ?
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: Ovoidhunter]
#26971239 - 10/05/20 08:15 PM (3 years, 3 months ago) |
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Just use LME, fuck sugar.
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Ovoidhunter
Buttery Crescent



Registered: 09/17/16
Posts: 2,016
Last seen: 2 years, 8 months
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#26971244 - 10/05/20 08:17 PM (3 years, 3 months ago) |
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I've been using malt extract that should work the same also shouldn't it? I probably won't do it on a large scale until I play around with it for a bit.
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