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Celestialexplorer1


Registered: 03/25/20
Posts: 1,803
Loc: Floating in a tin can
Last seen: 2 months, 13 days
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Re: Cultivation General Discussion [Re: VolcanoCybe]
#26955211 - 09/25/20 10:43 PM (3 years, 4 months ago) |
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Your friend has shared a link to a Home Depot product they think you would be interested in seeing.
6 in. Hole Dozer Bi-Metal Hole Saw
https://www.homedepot.com/p/202327766
--------------------
 To spend just one moment in eternity
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TedsDead



Registered: 01/03/17
Posts: 4,998
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If your careful and get one without paint u can return it after u cut the holes thats what I used for my SAB
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 14 days, 1 hour
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Re: Cultivation General Discussion [Re: TedsDead]
#26955655 - 09/26/20 07:00 AM (3 years, 4 months ago) |
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Wow! Wish I had tried the hot coffee can before. The little tomato paste can went thru like butter! Feels good! Uh huh, uh huh!  The flame is 2" away from the side of the 90 Qrt SAB. The box holding the torch is what petri dishes came in. Compass and triangle from a previous life...
Oh the wife said I could reduce the size of the hole with tape, cause now actually going thru the motions the hole feels a bit bigger than needed.
Edited by Inthepit (09/26/20 07:09 AM)
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VolcanoCybe
Stranger



Registered: 07/15/20
Posts: 100
Last seen: 1 year, 8 months
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Quote:
Celestialexplorer1 said: Your friend has shared a link to a Home Depot product they think you would be interested in seeing.
6 in. Hole Dozer Bi-Metal Hole Saw
https://www.homedepot.com/p/202327766
Dude, right on. I definitely did not wanna blow torch my holes. Precision is so much better for the mindset. How else do you modify the tub? Do you need to do anything besides the two holes? Or do you need to fasten down the test tube rack to the lid. Also how does your friend Sterilize prior to using the SAB?
-------------------- VolcanoCybe
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Quote:
Celestialexplorer1 said: Speaking of SABs I made this about a year ago and have been very happy with it. It looks this those holes most people cut would hurt your arms or you would actually have to hold you arms up.
 I went to Home Depot and got toilet flanges and bolted them on and sealed with clear silicone caulk and I can lay my arms in the holes and with my arms in there there is only a small gap where air could get in if it ever had the chance. It makes things much easier. Just a suggestion. I have a flow hood filter sitting in my grow room now I need to make the housing once the fan comes in. Then this is probably going in the trash. Or Iβll use it to store my plates.
counter-intuitive. you want big holes. small holes create air current as your arms go in/out. big holes don't buffet the air. air will be stiller with larger holes than smaller. but probably didn't matter all that much. I've used much shitter SABs with great success.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Cultivation General Discussion [Re: Lenz]
#26955775 - 09/26/20 09:04 AM (3 years, 4 months ago) |
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Quote:
Lenz said: Would you G2G these jars once they're fully colonized?
They were inoculated with LI I made with one of the few cultures I was confident was clean. Only potential problem is that when I was shaking up the LI I used a jar with a plastic lid and a little bit of water would come out. Don't think that really let anything into the LI though.
Also, if I'm inoculating with LI, is it fully colonized when it looks fully colonized on the outside or should I give it another day or two to fully consolidate on the inside?
This was the plate:
 
These are the jars:
 
 

 
To my eye they look pretty damn clean but this will be my first time G2Ging and want to be sure they're okay before I go ahead. There are a few very very tiny wet presumably bacterial spots but they're barely noticeable.
Honestly they look like they could have been finished for days already if you shook them a week ago. They look kind of consolidated in some parts and will like be a bitch to shake. Could also have some bacteria hiding in there , itβs hard to tell for sure but at this point I think itβs worth considering.
I would spawn those but not G2G. You shouldnβt G2G things you think may be bacterial.
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Lenz
Misunderestimated


Registered: 08/17/20
Posts: 692
Loc: Fungistan
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Re: Cultivation General Discussion [Re: Pastywhyte]
#26955812 - 09/26/20 09:43 AM (3 years, 4 months ago) |
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Quote:
Pastywhyte said:
Honestly they look like they could have been finished for days already if you shook them a week ago. They look kind of consolidated in some parts and will like be a bitch to shake. Could also have some bacteria hiding in there , itβs hard to tell for sure but at this point I think itβs worth considering.
I would spawn those but not G2G. You shouldnβt G2G things you think may be bacterial.
Thanks for the reply Pasty. Munchauzen's tek says it isn't necessary to shake LI jars since it should be distributed throughout the grain evenly already so that's why I skipped it but it does look like a shake would've been useful.
I don't have too many plates that I'm super confident in right now and I do have a fuckload of jars to inoculate so I might take the risk and G2G these still unless you think that's an outright bad idea. If they are bacterial, will the bacteria simply multiply exponentially through the new uncolonized grain of the receiving jar? If that's the case it should be easier to spot right?
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: Cultivation General Discussion [Re: Lenz] 1
#26955844 - 09/26/20 10:08 AM (3 years, 4 months ago) |
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Quote:
Lenz said:
Quote:
Pastywhyte said:
Honestly they look like they could have been finished for days already if you shook them a week ago. They look kind of consolidated in some parts and will like be a bitch to shake. Could also have some bacteria hiding in there , itβs hard to tell for sure but at this point I think itβs worth considering.
I would spawn those but not G2G. You shouldnβt G2G things you think may be bacterial.
Thanks for the reply Pasty. Munchauzen's tek says it isn't necessary to shake LI jars since it should be distributed throughout the grain evenly already so that's why I skipped it but it does look like a shake would've been useful.
I don't have too many plates that I'm super confident in right now and I do have a fuckload of jars to inoculate so I might take the risk and G2G these still unless you think that's an outright bad idea. If they are bacterial, will the bacteria simply multiply exponentially through the new uncolonized grain of the receiving jar? If that's the case it should be easier to spot right?
lets read the full quote from my tek
Quote:
Q: do I shake at 30%? A: ideally no, you should have shaken it well enough to evenly coat everything when you poured it, and that will result in even colonization. however, if you do end up with uneven growth, you can indeed shake at 30%.
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Lenz
Misunderestimated


Registered: 08/17/20
Posts: 692
Loc: Fungistan
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Re: Cultivation General Discussion [Re: Munchauzen]
#26955892 - 09/26/20 10:45 AM (3 years, 4 months ago) |
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Somehow completely missed that when I read through the tek lol, thanks for the clarification. Should I try and shake it now or will the myc eventually get there, albeit slowly?
Edit: Guess I'm not too clear on what consolidated means, does that mean the myc has stopped looking for more nutrition and has settled down?
Edited by Lenz (09/26/20 10:48 AM)
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Huskies
Boop More Snoots



Registered: 03/22/16
Posts: 1,048
Last seen: 1 day, 3 hours
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Re: Cultivation General Discussion [Re: Lenz]
#26955918 - 09/26/20 11:08 AM (3 years, 4 months ago) |
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So I had a leftover cake that had been forgotten. I soaked it in water in the jar, but that ended up staying in there for three days.
It does not smell rotten, it was filled with metabolites, the water was golden color. It has a very strong, pungent smell, but its all mushroom. It's in a tiny container, been a few days, no signs of pins yet.
Think I can get something out of it?
My other option, since I was gifted some clones that are about to go to their biggirl pots, I was thinking of crumbling the make and adding it to the bottom of the soil, create some mushroom compost or hopefully a proper mycelial network for my baby roots.
Anyone done something with that? If the cake isnt ideal for the above, I might use the leftover agars after I transfer them, they have been a bit neglected.
Basically, I want to see if its possible for any beneficial symbiosis between the mycelium and weed root system. If anyone has any input on that let me know.
-------------------- I call them Huskies cause you tell them to go "Mush! Mush""
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Gan
Wielder of Narya



Registered: 08/26/19
Posts: 927
Loc: Valinor
Last seen: 5 months, 28 days
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Re: Cultivation General Discussion [Re: Huskies]
#26955924 - 09/26/20 11:12 AM (3 years, 4 months ago) |
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Could you get something out of it? Yeah, probably. I've fruited some fucked spawn (only really bacterial stuff... I dont mess with any mold contamed spawn) and gotten something from it.
Will it be worth it? More than likely, not one bit. Especially if it's just one half pint cake. Idk how bad it is with no pictures, but if it's as bad as I think based off your description, you'll be lucky to get enough for a good dose. So not worth the time imo.
But, if it's for an experiment, go for it.
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Aablmd82
Mushroom mushroom man
Registered: 04/26/20
Posts: 71
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Inocuole]
#26955978 - 09/26/20 12:01 PM (3 years, 4 months ago) |
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What does a long term game plan look like in terms of βstrainβ storage and propagation, say starting from a clone? I understand that agar/slants are good for medium/long term when refrigerated, but what are the preferred ways to keep a master culture going while using that culture in current grows?
Since g2g moves the mushrooms closer to senescence, but not agar to agar, should one just keep a good supply of agar plates with desired strains? Or is LC the preferred way, with still a good supply of plates? Itβs probably all up to user choice, but I just wanted to hear some thoughts.
Edit: thanks guys, that was perfect
-------------------- Clone -> agar -> UnBODified monotub (first attempt, left unattended a week before harvesting)
Thanks BOD π
Edited by Aablmd82 (09/29/20 02:10 PM)
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TedsDead



Registered: 01/03/17
Posts: 4,998
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Re: Cultivation General Discussion [Re: Aablmd82]
#26955989 - 09/26/20 12:07 PM (3 years, 4 months ago) |
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-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 4 hours, 4 minutes
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Re: Cultivation General Discussion [Re: TedsDead]
#26956142 - 09/26/20 01:33 PM (3 years, 4 months ago) |
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Yep thatβs the thread for sure.
All about master plates. So crazy to think about how a couple microscopic spores can be expanded into hundreds of pounds of mushrooms in a few months.
--------------------
LAGM2020     
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MLPismyOPSEC
That One Ponyfucker


Registered: 11/13/18
Posts: 884
Loc: Equestria? Mordor? Wester...
Last seen: 11 days, 10 hours
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Re: Cultivation General Discussion [Re: starbones]
#26956179 - 09/26/20 01:57 PM (3 years, 4 months ago) |
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Quote:
starbones said: Got five tubs of WBS soaking right now and a few questions.
Is it really necessary to rinse them? I never rinsed my oats and they were pretty dirty from being from a grain elevator. The black floating sunflower seeds, they any benefit to the mycellium or just toss the fuckers? There's a couple peanuts in there. Necessary to remove them or will they be fine?
Fuck rinsing, it's a waste of water and time. I used to like rinsing until i realized none of the big guys (Youtubers realistically) don't rinse. They soak, load, and go. Same with floaters, leave em in, it's not hurting anything. If anything, it's hurting your amount of spawn and your wallet. In one 50 lb bag of WBS, i threw out 7 lbs of floaters... That's significant, and i was being stingy on how many floaters i took out.
Quote:
starbones said: Oats were grand but if this works better I'll retool specifically for it for sure.
Extremely fucking annoying, but worth the hassle IMO. Colonizes so much faster
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starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 18 days
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Re: Cultivation General Discussion [Re: MLPismyOPSEC]
#26956211 - 09/26/20 02:27 PM (3 years, 4 months ago) |
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Eh, we'll see.
I'm really in the "Never again" camp with it WBS right now due to the hassle v.s. oats. I need to make enough spawn to keep 40-70 monotubs going at anytime and I'm starting over from scratch here.
The stink after their 24 hour soak though, my fucking god the smell shits up my kitchen something terrible. The WBS actively fermenting when I open the lid to a tub to drain it is wild though.
I've got to find some without these random peanuts in it though. If the mycellium won't colonize a peanut that's fine, while in a jar. For me I'm looking at it as problematic when I spawn it as an uncolonized peace of food must be a mold target.
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MLPismyOPSEC
That One Ponyfucker


Registered: 11/13/18
Posts: 884
Loc: Equestria? Mordor? Wester...
Last seen: 11 days, 10 hours
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Re: Cultivation General Discussion [Re: starbones]
#26956220 - 09/26/20 02:36 PM (3 years, 4 months ago) |
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I was in the same boat when i first did WBS too, i HATED it, but seeing how quick it colonized and switching to a soak prep made it totally different. Made some strainers out of 5 gallon buckets, and i only soak for 12-16 hours. If you have a Wally World near you, they sell Pennington's which has been peanut free, for me, and maybe half a dozen small twigs.
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JHOVA
Post whore


Registered: 02/17/17
Posts: 4,727
Loc:
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Re: Cultivation General Discussion [Re: MLPismyOPSEC]
#26956231 - 09/26/20 02:49 PM (3 years, 4 months ago) |
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A twenty minute hot soak works fine for wbs. No rinse and a 2 hour steam dry until they are dry on the outside.
-------------------- π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
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Yeetusdeetus


Registered: 11/23/19
Posts: 1,242
Last seen: 5 hours, 24 minutes
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Re: Cultivation General Discussion [Re: JHOVA]
#26956539 - 09/26/20 06:34 PM (3 years, 4 months ago) |
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Does anybody know why wbs colonizes so much faster than straight millet, milo, or wheat?
--------------------

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Funky Monkey
Human Suppository


Registered: 05/14/19
Posts: 1,099
Loc: In your MOM's poop shoot
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Re: Cultivation General Discussion [Re: Yeetusdeetus]
#26956599 - 09/26/20 07:29 PM (3 years, 4 months ago) |
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If it's anything it's the varying sizes of grains.
However in the end I think it's fairly negligible. People's individual cultures will play more of a roll in the performance department.
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