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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: mushboy]
#26948478 - 09/21/20 08:42 PM (3 years, 4 months ago) |
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Quote:
mushboy said: he said strains 
I may be wrong but I think a 'strain' is any dikaryotic myc in which case the poster 'inadvertently' used the term correctly since a bunch of 'strains' will be colonizing and fruiting on those cakes
-------------------- Cakes inside Water Tub
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rickomalley238
Stranger
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Re: Cultivation General Discussion [Re: mushboy]
#26948483 - 09/21/20 08:46 PM (3 years, 4 months ago) |
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Quote:
mushboy said: he said strains 
Is GT not a strain different than PE different than PF classic different than APE? I know a cube is a cube, but isn't that like saying THC is THC in regards to bud? What am I missing here?
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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THC in weed would be equivalent to Psilocybin in mushrooms. White Widow is a strain of weed, Golden Teacher is a variety of Psilocybe Cubensis mushroom species. When two spores mate they create dikaryotic mycelium which is able to produce fruits (or not) and this live culture is called a strain or strains depending on how a colony is growing or how many strains are in a culture. I hope I'm correct on this if not I'm sure someone will chime in to correct me.
-------------------- Cakes inside Water Tub
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: Mateja]
#26948533 - 09/21/20 09:37 PM (3 years, 4 months ago) |
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just thoroughly flame the needle. the heat will kill whatever spores may linger inside the needle. hence us flaming the needle b4 use. common practice.
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Funky Monkey
Human Suppository


Registered: 05/14/19
Posts: 1,099
Loc: In your MOM's poop shoot
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Re: Cultivation General Discussion [Re: Mateja]
#26948534 - 09/21/20 09:39 PM (3 years, 4 months ago) |
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Quote:
Mateah said: THC in weed would be equivalent to Psilocybin in mushrooms. White Widow is a strain of weed, Golden Teacher is a variety of Psilocybe Cubensis mushroom species. When two spores mate they create dikaryotic mycelium which is able to produce fruits (or not) and this live culture is called a strain or strains depending on how a colony is growing or how many strains are in a culture. I hope I'm correct on this if not I'm sure someone will chime in to correct me.
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TedsDead



Registered: 01/03/17
Posts: 4,998
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Re: Cultivation General Discussion [Re: Mateja]
#26948620 - 09/21/20 11:01 PM (3 years, 4 months ago) |
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Quote:
Mateah said:
Quote:
mushboy said: he said strains 
I may be wrong but I think a 'strain' is any dikaryotic myc in which case the poster 'inadvertently' used the term correctly since a bunch of 'strains' will be colonizing and fruiting on those cakes 
Yes, but I dont think thats how they understood it hence gt and redspore being different "strains"
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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LemonTekno
Stranger
Registered: 08/22/20
Posts: 121
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Re: Cultivation General Discussion [Re: Gan]
#26948760 - 09/22/20 03:20 AM (3 years, 4 months ago) |
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24 hours later and they indeed did. Oh well, not the end of the world I suppose, just annoying to harvest.
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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 14 days, 4 hours
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Re: Cultivation General Discussion [Re: Mateja]
#26948795 - 09/22/20 05:03 AM (3 years, 4 months ago) |
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Quote:
mushboy said: he said strains 
Quote:
Rick...Right, I just don't want to use the same needle and inject a different strain than I'm thinking, ya know?
Quote:
Mateah said: I may be wrong but I think a 'strain' is any dikaryotic myc in which case the poster 'inadvertently' used the term correctly since a bunch of 'strains' will be colonizing and fruiting on those cakes 
OOh ooh, I wanna play...
So he can't be injecting "dikaryotic myc", that would be illegal. He must have spores and so it's different varieties he's injecting.
Mushboy yer sooooo funny!
Edited by Inthepit (09/22/20 07:24 AM)
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MLPismyOPSEC
That One Ponyfucker


Registered: 11/13/18
Posts: 884
Loc: Equestria? Mordor? Wester...
Last seen: 11 days, 13 hours
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Quote:
rickomalley238 said: Is GT not a strain different than PE different than PF classic different than APE? I know a cube is a cube, but isn't that like saying THC is THC in regards to bud? What am I missing here?
No, GT is not a different strain. They are different varieties of the same genus (hence cube is a cube), and the easiest way to describe it is that Cubensis is the strain, GT is the variety. It does not work like weed. There's a lot of debate whether different varieties produce different results, and it likely varies person to person.
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LoadedFish
Psilocybin Enthusiast


Registered: 07/12/20
Posts: 107
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Re: Cultivation General Discussion [Re: MLPismyOPSEC]
#26948993 - 09/22/20 09:16 AM (3 years, 4 months ago) |
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Noob question here, will contammed agar plates / jars spread their contams to other plates and jars? All of my plates look like healthy myc but I know my sterile procedure was messy for one of them and it’s making me nervous keeping it around.
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: LoadedFish]
#26949033 - 09/22/20 09:49 AM (3 years, 4 months ago) |
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No! plates won't contaminate one another, if they do then you're not using them correct. Gl
-------------------- Cakes inside Water Tub
Edited by Mateja (09/22/20 12:10 PM)
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Tweeq
Tweeq of Nature


Registered: 06/07/18
Posts: 2,043
Loc: Netherlands
Last seen: 10 hours, 48 minutes
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Re: Cultivation General Discussion [Re: MLPismyOPSEC]
#26949136 - 09/22/20 11:03 AM (3 years, 4 months ago) |
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Quote:
MLPismyOPSEC said:
Quote:
rickomalley238 said: Is GT not a strain different than PE different than PF classic different than APE? I know a cube is a cube, but isn't that like saying THC is THC in regards to bud? What am I missing here?
No, GT is not a different strain. They are different varieties of the same genus (hence cube is a cube), and the easiest way to describe it is that Cubensis is the strain, GT is the variety. It does not work like weed. There's a lot of debate whether different varieties produce different results, and it likely varies person to person.
This is not entirely accurate I'm afraid..
-Psilocybe would be the genus -Cubensis the species -GT or RW the variety -Now, when just two monokaryons (out of a spore) meet, that would be a single strain.
Idk if I have explained this 100pct accurately but this should give you an idea.
So if you grow from spores there are likely thousands of strains on you agar plate.
Transferring tiny bits over and over will reduce the number of strains.
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shroomshine
Captain



Registered: 03/11/20
Posts: 247
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Re: Cultivation General Discussion [Re: mushboy]
#26949281 - 09/22/20 12:40 PM (3 years, 4 months ago) |
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Quote:
mushboy said: he said strains 
after reading so much on shroomery that's exactly how I feel every time I read "strain"
-------------------- bang bang free pour gang Fuck. Oats. Shroomery.org search engine
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Jbets75
Stranger


Registered: 06/21/20
Posts: 97
Loc: Northeast Florida
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Re: Cultivation General Discussion [Re: shroomshine]
#26949370 - 09/22/20 01:35 PM (3 years, 4 months ago) |
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This might be a dumb question but gona ask anyways. I have several sterilized gratin jars that I didn’t have time to knock up. I wanted to know the shelf life. Ones been sitting almost 2 weeks. Rye still looks healthy and no visible signs of any bacterial growth. Others for a week. Kept em at room temp which is 72. Is it better to just toss them or can I perhaps soak em again for a short time and re-sterilize.
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A.k.a
Stranger



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Re: Cultivation General Discussion [Re: Jbets75]
#26949377 - 09/22/20 01:39 PM (3 years, 4 months ago) |
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They should be fine to use like normal.
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LAGM2020     
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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
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Re: Cultivation General Discussion [Re: Tweeq]
#26949410 - 09/22/20 01:59 PM (3 years, 4 months ago) |
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Quote:
Tweeq said:
-Psilocybe would be the genus -Cubensis the species -GT or RW the variety -Now, when just two monokaryons (out of a spore) meet, that would be a single strain. So if you grow from spores there are likely thousands of strains on you agar plate. Transferring tiny bits over and over will reduce the number of strains.
Thanks Tweeq prestigious winner!
And...taking a clone would grow a single strain or are there still many strain possibilities?
Take a tour through the sewer Don't strain your brain, paint a train You'll be singin' in the rain Said don't stop to punk rock...
Edited by Inthepit (09/22/20 02:11 PM)
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tryptkaloids
Learner



Registered: 02/08/15
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Re: Cultivation General Discussion [Re: Inthepit]
#26949468 - 09/22/20 02:26 PM (3 years, 4 months ago) |
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A clone is still multiple strains.
Unless it is an isolate then it is a monoculture
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Tweeq
Tweeq of Nature


Registered: 06/07/18
Posts: 2,043
Loc: Netherlands
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Re: Cultivation General Discussion [Re: tryptkaloids]
#26949472 - 09/22/20 02:29 PM (3 years, 4 months ago) |
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FriedEgg



Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
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Re: Cultivation General Discussion [Re: Tweeq] 3
#26949676 - 09/22/20 04:10 PM (3 years, 4 months ago) |
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did anyone else see biden stopped by the AA factory to make sure shroomerites have enough pressure cookers. i was on the fence but he definitely has my vote now 
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
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Re: Cultivation General Discussion [Re: FriedEgg]
#26949770 - 09/22/20 05:05 PM (3 years, 4 months ago) |
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Thems is shiny
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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