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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 10 hours
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Re: Cultivation General Discussion [Re: redbee]
#26944590 - 09/19/20 05:12 PM (3 years, 4 months ago) |
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As long as you can handle them it shouldn't be too hot Never timed it
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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redbee
prole spore threat


Registered: 03/30/20
Posts: 175
Last seen: 1 year, 7 months
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Re: Cultivation General Discussion [Re: tryptkaloids]
#26944694 - 09/19/20 06:33 PM (3 years, 4 months ago) |
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Gotcha, thanks trypt.
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redbee's LAGM 2021
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Dj_karma_magnet


Registered: 04/03/19
Posts: 203
Last seen: 4 months, 6 days
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Re: Cultivation General Discussion [Re: redbee]
#26944911 - 09/19/20 08:48 PM (3 years, 4 months ago) |
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Just made a stupid mistake, I have a small space heater I turn on in the closet sometimes to keep ideal temps. Left it on for about a half hour and it was 103 in their. I turned it off immediately and opened the door and it is now down to 87. Are my cakes fucked? They have already had the first flush
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 10 hours
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Probably not. Cubes are a tropical species. Prolonged high temp can promote bacterial growth during colonization.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: tryptkaloids]
#26945478 - 09/20/20 03:21 AM (3 years, 4 months ago) |
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Quote:
tryptkaloids said: Prolonged high temp can promote bacterial growth during colonization
I sometimes ask myself how bacterial colonies spread inside a porous semi dry-ish BRF substrate considering bacteria is dependent on moving water/liquids for mobility since they can't walk around on their own like mushroom and mold mycelium can. But then again what the hell do I really know about these microbes
-------------------- Cakes inside Water Tub
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Tweeq
Tweeq of Nature


Registered: 06/07/18
Posts: 2,043
Loc: Netherlands
Last seen: 7 hours, 29 minutes
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Re: Cultivation General Discussion [Re: Mateja]
#26945486 - 09/20/20 03:38 AM (3 years, 4 months ago) |
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Happy 9/20 Magic Mushroom Day everybody!
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: Mateja]
#26945487 - 09/20/20 03:41 AM (3 years, 4 months ago) |
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Quote:
Mateah said:
Quote:
tryptkaloids said: Prolonged high temp can promote bacterial growth during colonization
I sometimes ask myself how bacterial colonies spread inside a porous semi dry-ish BRF substrate considering bacteria is dependent on moving water/liquids for mobility since they can't walk around on their own like mushroom and mold mycelium can. But then again what the hell do I really know about these microbes 
FWIW, I've cleaned a bunch of pretty disgusting cultures over the years using the the last tek in my sig which consists of making a substrate of BRF/verm in small containers, as simple as that. I'm talking about agar cultures with the type of bacteria that wouldn't quit no matter what (countless transfers, sandwich tek, hot pour...). Seeing those cultures get back to health in a matter of days was quite the "WTF" moment when I first used this procedure.
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Nobler Hino
a dojo and a forge?!


Registered: 08/29/15
Posts: 1,780
Loc: Deep Ellum
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Re: Cultivation General Discussion [Re: Josex]
#26945507 - 09/20/20 04:38 AM (3 years, 4 months ago) |
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If bacteria is present, a whole generation of bacteria can double in as low as 20 mins. This is under normal circumstances. It's pretty amazing how a biopsy puck can combat that speed.
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   "The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.” – Maria Sabina
Edited by Nobler Hino (09/20/20 06:10 AM)
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 7 hours
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Re: Cultivation General Discussion [Re: Nobler Hino]
#26945515 - 09/20/20 04:51 AM (3 years, 4 months ago) |
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i do bags like this twice a week and never have a problem.....
     
I didnt do this just for you guy by the way it for a 3 monotub per cook thread im doing
Regarding PE#6 has anyone ever had a batch that is as strong as PE?
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rickomalley238
Stranger
Registered: 08/09/20
Posts: 414
Last seen: 6 months, 20 days
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Re: Cultivation General Discussion [Re: Edmunter]
#26945937 - 09/20/20 10:33 AM (3 years, 4 months ago) |
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Here is a picture of the second flush on my cake. One thing I am curious about: I made absolutely sure not to waterlog this cake, I misted it maybe 2-3x a day and only until it was glistening and then stopped. It produced short fat mushrooms, but I notice one of the mushrooms has its breaking and it is a very small mushroom; but again I didn't waterlog it this time. I'm wondering, does anyone know why the veil broke on such a small mushroom? Could it be genetics? Other factors at play?
You can't really see it in the picture, but the smaller mushroom's veil is breaking. Picture:
Edited by rickomalley238 (09/20/20 10:34 AM)
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Celestialexplorer1


Registered: 03/25/20
Posts: 1,803
Loc: Floating in a tin can
Last seen: 2 months, 13 days
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Re: Cultivation General Discussion [Re: Edmunter]
#26945952 - 09/20/20 10:40 AM (3 years, 4 months ago) |
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Quote:
Edmunter said:
Regarding PE#6 has anyone ever had a batch that is as strong as PE?
Just tried the natalensis and I have to say I was expecting an identical to cube experience as far as dosage but I was harvesting and had about three medium sized mushrooms but solid through and through. I didn’t weight just ate them and I don’t believe I’ve ever had cubes do that from just three fruits but it’s far from a worthy description of dosage because I didn’t weight them.
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 To spend just one moment in eternity
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Quote:
rickomalley238 said: Here is a picture of the second flush on my cake. One thing I am curious about: I made absolutely sure not to waterlog this cake, I misted it maybe 2-3x a day and only until it was glistening and then stopped. It produced short fat mushrooms, but I notice one of the mushrooms has its breaking and it is a very small mushroom; but again I didn't waterlog it this time. I'm wondering, does anyone know why the veil broke on such a small mushroom? Could it be genetics? Other factors at play?
You can't really see it in the picture, but the smaller mushroom's veil is breaking. Picture:

Sometimes they also just grow weird, they shrooms
-------------------- Cakes inside Water Tub
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Cultivation General Discussion [Re: Mateja]
#26946006 - 09/20/20 11:14 AM (3 years, 4 months ago) |
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Not all bacteria is static, some bacteria is motile and have different means of movement. Bacillus species have a flagellum and are therefore motile. They don’t move fast but to say they can’t is false.
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rickomalley238
Stranger
Registered: 08/09/20
Posts: 414
Last seen: 6 months, 20 days
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Re: Cultivation General Discussion [Re: Mateja]
#26946007 - 09/20/20 11:15 AM (3 years, 4 months ago) |
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Appreciate it. ^
I just went to harvest these, and I notice a third flush pinset formed on the bottom of the cake with some fuzzy feet. I just dunked the cake to prep for 3rd flush. If you guys were me, when you put the cake back in the SGFC, would you flip it so the pinset isn't on the bottom this time? (there is less vermiculite layer on the bottom, however). Or would you put it back on the bottom?
Thanks
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Cultivation General Discussion [Re: Pastywhyte]
#26946033 - 09/20/20 11:31 AM (3 years, 4 months ago) |
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Quote:
Pastywhyte said: Not all bacteria is static, some bacteria is motile and have different means of movement. Bacillus species have a flagellum and are therefore motile. They don’t move fast but to say they can’t is false.
Yes it could technically be wrong to say they're not mobile, but are they mobile without the presence of liquids/water? As a BRF substrate for example which basically resembles somewhat damp sand and in my head at least I have trouble visualizing how a bacterial colony would be able to colonize such a substrate in any effective way. Not saying it can't be so, like I said I don't know much more except the most basic things about these microbes. Tho overly saturated cakes or fully rehydrate ones seem to on paper at least to provide least obstacles for a bacterial colony.
-------------------- Cakes inside Water Tub
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: Mateja]
#26946324 - 09/20/20 02:41 PM (3 years, 4 months ago) |
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I would be interesting to observe bacteria with a scope on different substrates to see how they move/interact in each environment.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#26946473 - 09/20/20 04:14 PM (3 years, 4 months ago) |
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Are you saying there is no water in brf cakes? Just because they ain’t swimming in liquid doesn’t mean there isn’t enough for it to move and multiply.
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alaskappalachian
Entitiologist

Registered: 10/22/19
Posts: 1,674
Loc: The 49th Dimension
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Re: Cultivation General Discussion [Re: Pastywhyte]
#26946586 - 09/20/20 05:33 PM (3 years, 4 months ago) |
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The flagellar system is incredibly sophisticated and bacteria can do some serious tricks with it to navigate towards nutrient attractants.
-------------------- "First we build the tools, then they build us." THE 49th MYCOJOURNAL: Exotics, Auroras, and Entities
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Dj_karma_magnet


Registered: 04/03/19
Posts: 203
Last seen: 4 months, 6 days
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How long generally until 2nd flush with grain spawn? What should I do with all these little pins that never formed on my cake?
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rickomalley238
Stranger
Registered: 08/09/20
Posts: 414
Last seen: 6 months, 20 days
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I dunked for my third flush today, this time I left pins on the cake because I thought they would be for the third flush. Anyways, I just removed the cake from the dunk and all the pins are deep blue and likely dead.. why did this happen? I thought pins survive a dunk and are necessary for the next flush?
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