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OfflineOvoidhunter
Buttery Crescent
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Registered: 09/17/16
Posts: 2,016
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Re: Cultivation General Discussion [Re: Anastomosis]
    #26905806 - 08/29/20 10:33 AM (3 years, 4 months ago)

Quote:

Anastomosis said:
This guy c10 (who needs to change his name to Dr. Bad cause he's a badass) has an awesome thread on it.

https://www.shroomery.org/forums/showflat.php/Number/23653379





Hey anastomisis, Im finally getting around to do the thing I asked you about.. :wink:

Its gonna be epic.


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InvisibleMunchauzen
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Registered: 06/22/11
Posts: 14,342
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Re: Cultivation General Discussion [Re: starbones]
    #26905861 - 08/29/20 11:20 AM (3 years, 4 months ago)

Quote:

starbones said:
Quote:

jbgtaa said:
Just take everything down, clean everything with a good mold killer, you could even multi fuck it and use fog and decissant, scrub the air if you have a flow hood or place a good air purifier in the room on high for a few weeks, and start from spore. If you pulled 25lb im sure you're using a really good clone but its probably also time to switch it up given the circumstance. Start from spore and just really scrub your whole area, take everything out and give it a real good scrubbing and you should be fine.




Negative on starting from spore. I've got gallons of LC that tests fine on agar, just checked it a week ago when this problem started occurring. I didn't have trouble until I started using spawn bags because 140+ jars was getting old and I guarantee that's where my contamination came in. One tub went shitty and I did not notice until it went green and the fucker was downwind (upwind? in front of the fan) of everything else. I've got 45 monotubs fruiting right now between 1st and 2nd flush out of my original 60+ and out of these monos any that are giving their 1st will be giving their last.

Agreed on the fogger, I've got a 2 gallon sprayer full of Moldex ready to go and I'll be renting a fogger full of concrobium to TRY and deal with the mold in the air, walls, floor.

Air, fresh and drier air is going to be a goal here but also going back to jars. I just trust em well enough.



Sounds like the issue is your technique, not your house. A bunch of chemicals, foggers, sprayers, are all just a bandaids on a broken bone. They won't help at all as mold yeast and bacteria are omnipresent no matter what we do. Since you recently switched to bags, perhaps you're not getting them properly sterilized. How long are you PCing your bags for? Are you inoculating in a SAB or flowhood?

Agreed with starting from scratch. Toss that LC, and don't use LC if you don't like contaminates. LI is way safer, IMHO.


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OfflineEdmunter
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Re: Cultivation General Discussion [Re: Edmunter] * 1
    #26905864 - 08/29/20 11:22 AM (3 years, 4 months ago)

Quote:

Edmunter said:
Wow, the best pinset ever to the extent im not sure there is room for them all.  Same culture in an unmodified box with 5 quarts spawn 1:2 cvg


I decided to give Pastys easy monos and booosh, look at this

Same 5 quarts 1:2
yesterday

Today






Update.  this clone has a habit of exploding over night and being 3 times as big suddenly.


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Offlinerickomalley238
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Registered: 08/09/20
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Re: Cultivation General Discussion [Re: Edmunter]
    #26905880 - 08/29/20 11:35 AM (3 years, 4 months ago)

Howdy folks. My 1/4 pint BRF jars fully colonized yesterday, and I am debating giving them a half week for consolidation time, or waiting for them to pin before dunking. Which do you guys recommend?


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OfflineAlsetAlokin
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Registered: 07/30/20
Posts: 182
Last seen: 3 years, 20 days
Re: Cultivation General Discussion [Re: Edmunter]
    #26905881 - 08/29/20 11:37 AM (3 years, 4 months ago)

Mold or myc?

No verm on top of the brf cakes. Planned on top fruiting.



Starting from syringes and all i can get is this type of growth which is hard to distinguish between bacteria, mold, and myc, so i'm asking for outside opinions.


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InvisibleMunchauzen
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Registered: 06/22/11
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Re: Cultivation General Discussion [Re: AlsetAlokin]
    #26905895 - 08/29/20 11:46 AM (3 years, 4 months ago)

Quote:

AlsetAlokin said:
Mold or myc?

No verm on top of the brf cakes. Planned on top fruiting.



Starting from syringes and all i can get is this type of growth which is hard to distinguish between bacteria, mold, and myc, so i'm asking for outside opinions.



I don't see any mold. the plates are hard to read because no contrast against the agar color, thats why I like food coloring. but honestly I don't see anything bad.


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: Munchauzen]
    #26905937 - 08/29/20 12:15 PM (3 years, 4 months ago)

Quote:








What's with those brown specks?


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OfflineAlsetAlokin
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Registered: 07/30/20
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Re: Cultivation General Discussion [Re: mushboy]
    #26905951 - 08/29/20 12:29 PM (3 years, 4 months ago)

Nute mix from agar, the bottom few plates usually have it.

Haven't found a solution for it yet other than maybe filtering my agar.

Edit: it may also be burnt sugars from boiling agar, these last batches may have been overheated. May try cold mixing next.


Edited by AlsetAlokin (08/29/20 12:32 PM)


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Offlinechromeleon
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Registered: 07/05/20
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Re: Cultivation General Discussion [Re: Munchauzen]
    #26905955 - 08/29/20 12:31 PM (3 years, 4 months ago)

I think I'm going to try doing some agar to agar transfers for the first time today. Do the spots I'm planning to transfer look like good candidates or should I wait for something more definite? I've read up on agar and transfers and stuff, just want to check my understanding and get another eye on my decisions. (I know the lack of contrast makes it tough to see. These are my first plates and I'm going to try a different agar recipe next time.)

Piece I'm trying to clone, targeting growth at 3 & 9:



I swabbed spores directly from a print to these. Both photos are oriented so the portion I want to select is at 3 o clock:





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Offlinetryptkaloids
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Registered: 02/08/15
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Re: Cultivation General Discussion [Re: chromeleon]
    #26905963 - 08/29/20 12:35 PM (3 years, 4 months ago)

Honestly they don't look great. But transfer them and see what they look like from there


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisibleMunchauzen
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Registered: 06/22/11
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Re: Cultivation General Discussion [Re: chromeleon]
    #26905966 - 08/29/20 12:40 PM (3 years, 4 months ago)

I don't like the look of the first plate at all. that wispy, scraggly growth coming from the center isn't normal. there is probably something meshed into that culture. third plate is overgrown. I can see it looks like you swabbed it but its grown out too much and everything has come together it looks like. with spore plates, you can take transfers anytime. I usually transfer from spores just a few days after germination.

transfers you could take from the 2nd plate


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OfflineJbets75
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Re: Cultivation General Discussion [Re: Munchauzen]
    #26905980 - 08/29/20 12:55 PM (3 years, 4 months ago)



How does this look guys. My first shoebox that I got to pin with this many but I feel like they are a bit on the dark side.  Any suggestions.  Thanks


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InvisibleMunchauzen
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Re: Cultivation General Discussion [Re: The Fresh Prints]
    #26905997 - 08/29/20 01:09 PM (3 years, 4 months ago)

Quote:

Genghis Chron said:

Second flush would have been nice too 🤦‍♂️



ya looks like they aborted.

also, don't tape your liners. if you have excess and don't want to cut it, fold it down the sides. the bag needs to shrink with the substrate to be most effective.


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OfflineEdmunter
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Re: Cultivation General Discussion [Re: Munchauzen]
    #26905999 - 08/29/20 01:11 PM (3 years, 4 months ago)

Quote:

Munchauzen said:
Quote:

Genghis Chron said:

Second flush would have been nice too 🤦‍♂️



don't tape your liners. if you have excess and don't want to cut it, fold it down the sides. the bag needs to shrink with the substrate to be effective.




Funny you should say that.  Ive been perfecting the hot knife cut around the edge using your chuck it in a sack method.  5 Minutes and its done......so good... thanks


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InvisibleMunchauzen
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Re: Cultivation General Discussion [Re: Edmunter]
    #26906002 - 08/29/20 01:12 PM (3 years, 4 months ago)

:munchdance:


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OfflineEdmunter
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Re: Cultivation General Discussion [Re: Munchauzen]
    #26906016 - 08/29/20 01:27 PM (3 years, 4 months ago)

:gaydance:


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OfflineInthepit
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Re: Cultivation General Discussion [Re: Edmunter]
    #26906039 - 08/29/20 01:41 PM (3 years, 4 months ago)

:rofl: OMG! You two are a crackup!


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OfflineGan
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Re: Cultivation General Discussion [Re: Inthepit]
    #26906100 - 08/29/20 02:13 PM (3 years, 4 months ago)

Do yall shake your g2g jars? I've got a bunch that are sitting at like 60%+ colonized 5 days out. I'm not sure if a shake would make it faster or slower to 100%.

Been so long since I last did g2g I really cant remember whether I used to shake.


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InvisibleMunchauzen
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Re: Cultivation General Discussion [Re: Gan]
    #26906102 - 08/29/20 02:14 PM (3 years, 4 months ago)

yeah shake at 30% it speeds things up a lot


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Offlinerickomalley238
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Registered: 08/09/20
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Last seen: 6 months, 20 days
Re: Cultivation General Discussion [Re: rickomalley238]
    #26906109 - 08/29/20 02:16 PM (3 years, 4 months ago)

Quote:

rickomalley238 said:
Howdy folks. My 1/4 pint BRF jars fully colonized yesterday, and I am debating giving them a half week for consolidation time, or waiting for them to pin before dunking. Which do you guys recommend?




well, my comment got completely buried, so just incase someone misses this, much appreciated.


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