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Invisiblecoversall
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Re: Cultivation General Discussion [Re: rickomalley238]
    #26881573 - 08/15/20 12:20 PM (3 years, 5 months ago)

imo that's the plastic getting a little burnt from the needle getting hot. It shouldn't be an issue.

If you're done inoculating and the jars are setup where they are going to stay then there is no trouble in airing the room out. If you've got a dry verm layer than that will filter out nasties.


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Offlinerickomalley238
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Re: Cultivation General Discussion [Re: coversall]
    #26881637 - 08/15/20 12:55 PM (3 years, 5 months ago)

Quote:

coversall said:
imo that's the plastic getting a little burnt from the needle getting hot. It shouldn't be an issue.

If you're done inoculating and the jars are setup where they are going to stay then there is no trouble in airing the room out. If you've got a dry verm layer than that will filter out nasties.




Thanks man.. was worried because I might of had the flame over it for a split second. Worst case scnario, would micro plastic fuck up inoculation or the mushrooms?


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InvisibleCaps McGee
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Re: Cultivation General Discussion [Re: rickomalley238]
    #26881643 - 08/15/20 12:59 PM (3 years, 5 months ago)

So long as it's sterile, should have no effect...

The alcohol will evaporate rather quickly, no need to bust out the blowers or anything


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Offlinestarbones
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Re: Cultivation General Discussion [Re: Caps McGee]
    #26881646 - 08/15/20 01:03 PM (3 years, 5 months ago)

Anyone know what that smell between flushes is? Nearly every monotub/shoebox I have done with oats/cvg gets it. Sharp, nearly ammonia like but quickly goes away once you air out the tub.

I shredded some spent subs today and none of them looked contaminated. Odd though, oats barely look digested either as if they had just come out of the PC.


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InvisibleCaps McGee
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Re: Cultivation General Discussion [Re: starbones]
    #26881649 - 08/15/20 01:08 PM (3 years, 5 months ago)

I'd say its stagnant water and a sign you need better fae/less initial hydration/ less frequent misting perhaps... remember that evaporation must occur for peak performance, and will not take place if the substrate is too wet, or has insufficient air exchange...  also will not take place if you're obsessively maintaining surface conditions... Ebb and flow (within reason)... sounds like stale air IMO, which IME, indicates one of the preceding 3... all resulting in "too wet for too long " conditions


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Offlinerickomalley238
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Re: Cultivation General Discussion [Re: Caps McGee]
    #26881653 - 08/15/20 01:10 PM (3 years, 5 months ago)

Quote:

Caps McGee said:
So long as it's sterile, should have no effect...

The alcohol will evaporate rather quickly, no need to bust out the blowers or anything



The plastic would have no effect, you mean?


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InvisibleCaps McGee
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Re: Cultivation General Discussion [Re: rickomalley238]
    #26881658 - 08/15/20 01:12 PM (3 years, 5 months ago)

Right... its plastic: like a tub

:rollsafe:

so long as there are no contaminant spores or bacterial colonies on the plastic, it will only sit there...

This whole thing is hypothetical, on the very limited (imo 0%) chance a small piece of heated/melted (likely sterilized) plastic making it's way into a sterilized jar


Edited by Caps McGee (08/15/20 01:20 PM)


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InvisibleJosex
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Re: Cultivation General Discussion [Re: Caps McGee]
    #26881666 - 08/15/20 01:23 PM (3 years, 5 months ago)

Is there a doctor in the house? What kind of medical grade tubing can take a PC cycle? I know there is silicone tubing, but what else?


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Re: Cultivation General Discussion [Re: Caps McGee]
    #26881678 - 08/15/20 01:28 PM (3 years, 5 months ago)

little photo bomb of some mean ass genetics ive been working on,started out with live culture.... 3 transfers in on malt agar these plates are on day 5!!! genetics are "Brazilian" super excited for these







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Offlinerickomalley238
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Re: Cultivation General Discussion [Re: Caps McGee]
    #26881741 - 08/15/20 02:01 PM (3 years, 5 months ago)

Quote:

Caps McGee said:
Right... its plastic: like a tub

:rollsafe:

so long as there are no contaminant spores or bacterial colonies on the plastic, it will only sit there...

This whole thing is hypothetical, on the very limited (imo 0%) chance a small piece of heated/melted (likely sterilized) plastic making it's way into a sterilized jar




I was thinking the mushrooms/fruits might have had plastic in them if that was the case. Similar to a water bottle containing plastic particles


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Offlinerickomalley238
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Re: Cultivation General Discussion [Re: Josex]
    #26881742 - 08/15/20 02:02 PM (3 years, 5 months ago)

Quote:

Josex said:
Is there a doctor in the house? What kind of medical grade tubing can take a PC cycle? I know there is silicone tubing, but what else?



pp5 plastic


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OfflineJbets75
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Re: Cultivation General Discussion [Re: swinghigh]
    #26881748 - 08/15/20 02:05 PM (3 years, 5 months ago)

Quick question.  I made MEA agar using a dehydrated Premix and the recipe called for 25grams per 500ml.  I used an MS syringe to agar.  I did one drop and then used an inoculation loop to spread it out.  On all 9 of my plates the liquid spore solution is still running across the top of the agar 3 days later.  Maybe I needed to press it into the agar more?  Or should I use less premix
Per 500 ml.  I had condensation on 2 plates which I thought I did a good job. 

Also I transferred an in vitro pin to one plate.  I had a hard
Time using the exact knife so I ripped it in half and off the brf with my sterile gloves.  And placed it on the agar and this is 6days later.  Any thoughts. 



Bottom of Petri


Pouring the agar in a SAB was so hard for me my first time. I think I’m gona do a no pour next time.  I manage a restaurant and we have pp5 soufflé cups.  I was wondering if I could use these cups in a pressure cooker. 

Thanks


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Re: Cultivation General Discussion [Re: Jbets75]
    #26881776 - 08/15/20 02:23 PM (3 years, 5 months ago)

i know from experience with MS syringes i didnt see any viable growth on my plates untill around day 7 or 8. define a bit more what you mean by "solution is still running across the top of the agar" if you spead out the drop on the agar im confuzed as to what is "running"........also did you allow your loop to fully cool down?

that pin sample on agar looks nice id get ready in a few days to take another sample and transfer to a fresh plate.

as for the pp5 souffle cups some people have used them in pressure cooker but advise you wrap them in a thick aluminum foil. check out this thread they talk about using them.

https://mycotopia.net/topic/86700-pp5-jars-touching-the-sides-of-the-pressure-cooker/


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InvisibleRoger Clemency
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Re: Cultivation General Discussion [Re: swinghigh]
    #26881795 - 08/15/20 02:30 PM (3 years, 5 months ago)

That pin should’ve grown out a lot more in 6 days. When I put a little pinner like that on agar it’s usually lookin like that in a day or two and yuuge (comparatively) by day 6.

It could be from from your agar being too nutritious maybe or it might be a slow colonizing culture or there’s bac I can’t see. Just throwing ideas out. I’m curious how it acts after 1st transfer


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Re: Cultivation General Discussion [Re: Roger Clemency]
    #26881809 - 08/15/20 02:38 PM (3 years, 5 months ago)

When I bought premix the instructions made it too nutritious. I ended up using like 60% of the recommended amount.


Looks like good growth off the pin though.


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OfflineOvoidhunter
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Re: Cultivation General Discussion [Re: A.k.a]
    #26881817 - 08/15/20 02:43 PM (3 years, 5 months ago)

What are some good examples of DIY bulk pasturization unit builds. I'm getting ready to build one.


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Re: Cultivation General Discussion [Re: Ovoidhunter]
    #26881849 - 08/15/20 03:07 PM (3 years, 5 months ago)

I made an inoculation loop out of twist tie from bread and attached it
To an exacto.  It wasn’t as firm as I’d hope. And yes I cooled
The loop on agar before spreading the spores.  I don’t know. Just seems like the liquid is still running on the agar when I tilt the plates.  I feel like the agar is too rigid.  Or I didn’t get it into the agar.


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Re: Cultivation General Discussion [Re: Jbets75]
    #26881903 - 08/15/20 03:44 PM (3 years, 5 months ago)

hmmm ya it shouldnt be runny still that kind of odd.

i dont think it has to do with your agar, ive always used pre mixed agar and malt extract without issue or it being to nutritious. that being said im going to contradic myself here and say some genetics will react differently to different types of agar and potency of the nutrient malt, potato ect.

as for your pin i agree with roger clemance it could be a handful of things not allowing that pin to take off on the agar.. but my recent plates of hulatua genetics that was taken from a stem sample is very slow after 4 transfers. its just its genetics i guess they arent as fast or they dont like the malt agar mix i have. where the Brazilian strain i showed earlier covered an entire plate in 5 days after 3 transfers


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Invisibledfwerydfhg
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Re: Cultivation General Discussion [Re: Josex]
    #26882055 - 08/15/20 05:30 PM (3 years, 5 months ago)

Quote:

Josex said:
Is there a doctor in the house? What kind of medical grade tubing can take a PC cycle? I know there is silicone tubing, but what else?




There's stuff called PharMed BPT or MasterFlex that can do repeated autoclave cycles (not sure both can, check the specs first). Not the cheapest stuff...


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InvisibleCaps McGee
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Re: Cultivation General Discussion [Re: swinghigh]
    #26882069 - 08/15/20 05:36 PM (3 years, 5 months ago)

Quote:

swinghigh said:
little photo bomb of some mean ass genetics ive been working on,started out with live culture.... 3 transfers in on malt agar these plates are on day 5!!! genetics are "Brazilian" super excited for these











I dont mean to potentially rain on your parade, but that thick and aggressive growth CAN indicate bacterial infection with the transfer... I'd be weary: hold it up to the light and make sure theres no translucent halo inside the culture anywhere, or even around the leading edge ... the intensity of the fingers reaching outward, coupled with inconsistencies in shape and form,  I'd take transfers from the very leading edge NOW and do so in the area of the plate center the longest, most consistent curvature/structure


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