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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: PsycDiesel]
    #26879826 - 08/14/20 09:44 AM (3 years, 5 months ago)

Like I said above, you can't sterilize cereal grain spawn jars by boiling them in a pot for two hours. This has nothing to do with the PF tek at all, not sure how to be any more clear.

PF tek (BRF) can be steam sterilized in a pot, follow the tek, I don't do cakes so I don't know what the protocol is off the top of my head.

If you are putting: rye, wheat, oats, or any other grain into a mason/glass jar with the intention of inoculatiing with either spores or culture tissue then you need to sterilize those grains. Boiling those grains (not BRF) for 2 hours is not sterilization. You cannot apply the PF tek to grain spawn production. Teo entirely different things.


Edited by Stipe-n Cap (08/14/20 09:51 AM)


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InvisibleRoger Clemency
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #26879837 - 08/14/20 09:49 AM (3 years, 5 months ago)

Mushrooms grow naturally without any intervention, cube myc fights for a cow pie and wins and random hominids find said mushrooms and eat them to expand their pea brains. But millions of trillions of spores never get to grow because they don’t land in the right spot or something else takes over first.

You would’ve killed some bad stuff in that 2 hour steaming, but not nearly all of it. You got lucky to have cube myc colonize it, just like the occasional spores get lucky in the world. But for consistent success you’ll need to properly sterilize


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: Roger Clemency]
    #26879839 - 08/14/20 09:53 AM (3 years, 5 months ago)

I don't see any colonized grains at all. He is going to have a bacterial bloom with zero mycelium. This is a certainty.


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OfflineFietchen
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #26879840 - 08/14/20 09:53 AM (3 years, 5 months ago)

Quote:

p9hu7 said:
You boiled for 2 hours, this is not sterilization.





2-4 hours


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Re: Cultivation General Discussion [Re: Fietchen]
    #26879842 - 08/14/20 09:55 AM (3 years, 5 months ago)

Holy shit. Carry on:facepalm:


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InvisibleRoger Clemency
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #26879865 - 08/14/20 10:07 AM (3 years, 5 months ago)

Quote:

p9hu7 said:
I don't see any colonized grains at all. He is going to have a bacterial bloom with zero mycelium. This is a certainty.




It’s all become clear to me now. We have two separate posters mentioning using a pot and no PC.

The one who claims some success is who I was thinking of - psych d - but he was using BRF cakes which makes sense.

Fitchen has the grain jars that were steamed for 2-4 hours and were nocc’d with a spore syringe. I’ll send what good vibes I can but even my godlike vibes probably won’t be enough to get you reasonably clean spawn. But once your PC is in you should be g2g


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OfflineTight Lunchbox
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Re: Cultivation General Discussion [Re: Fietchen]
    #26879878 - 08/14/20 10:14 AM (3 years, 5 months ago)

You have to pc grains at 15 psi for at least 90 minutes to be considered sterile. You did not do that, so you are very, very likely to end up with a bunch of nasty jars contaminated with bacteria. You should do some more reading.


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InvisibleStipe-n CapMDiscord
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Re: Cultivation General Discussion [Re: Roger Clemency]
    #26879882 - 08/14/20 10:15 AM (3 years, 5 months ago)

Yeah, they are both crossing the same wires though, I think.

Grain needs to be steam sterilized at the boiling temperature of water which is 100°c or 212°f. This temperature must be maintained for 8 to 10 hours to sterilize cereal grains at atmospheric temperature.  The catch is that it takes a while for the internal temperature of the jars to reach that temperature, so your actual sterilization time is much longer.

So in summary:steaming your jars for 2-4 hours is the exact same as just boiling them for hydration, it does nothing.

BRF or PF tek is different due to the type of organisms present. With BRF your primary concern is living bacteria that are easily killed with a bit of steam and a shortened duration of time. Grains however have bacteria containing endospore which are carried within their cell membranes, once the actual bacteria dies it leaves behind the endospore which are very resilient to heat. These little Fuckers need temperatures either higher than boiling under pressure or a far greater length of exposure.

This is a fact, it isn't a matter of debate. Cook longer, or grow bacteria.


Edited by Stipe-n Cap (08/14/20 10:24 AM)


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OfflinePsycDiesel
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #26879895 - 08/14/20 10:21 AM (3 years, 5 months ago)

So BRF cakes make a difference here?

Like I said I did a few without cooking them at all and none of them developed mycelium properly. Second time I cooked/boilded them for 1-2 hours and it grows just fine. Not contamination at all so far.

If BRF makes the difference, I will stay with that until I buy an pressure cooker.


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Re: Cultivation General Discussion [Re: PsycDiesel]
    #26879899 - 08/14/20 10:22 AM (3 years, 5 months ago)

Yes, it's different.


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Re: Cultivation General Discussion [Re: Stipe-n Cap]
    #26879924 - 08/14/20 10:35 AM (3 years, 5 months ago)

Brf is different for many reasons. But the main one is that the media in a brf jar is saturated with water and there are fewer/smaller air pockets in the mix compared to cereal grains. The heat is able to penetrate that type of media far more efficiently.


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OfflineThe Dalcassian
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Re: Cultivation General Discussion [Re: Smoothcat]
    #26879981 - 08/14/20 11:19 AM (3 years, 5 months ago)

Quote:

Smoothcat said:
Don’t look good mate tbh, my advice would be to try and top fruit that and hope for some kind of flush and grab some clones




Thanks for the input, would you put a layer of coir on top to top fruit? Or will it do so invitro?

I'm not too worried I still have the culture on 2 plates so I can just give it a couple more transfers and try again.
I try to not put all my hopes in one jar.


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Invisiblereplyom

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Re: Cultivation General Discussion [Re: The Dalcassian]
    #26880155 - 08/14/20 01:28 PM (3 years, 5 months ago)



Anyone know what's going on here? Around day 10 post-spawn these "pins" started to appear - at least I thought they were pins. But now it's clear that they are something else - just masses of fruiting body with no head. Y'all seen this before?


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Re: Cultivation General Discussion [Re: replyom]
    #26880179 - 08/14/20 01:42 PM (3 years, 5 months ago)

I’ve had similar things with bacterial tubs.

Usually the knots swell up and turn yellowish but then pins show up also.

It looks a little dry.


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Edited by A.k.a (08/14/20 01:43 PM)


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Invisiblereplyom

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Re: Cultivation General Discussion [Re: A.k.a]
    #26880182 - 08/14/20 01:44 PM (3 years, 5 months ago)

Interesting, thanks. How did you know the tubs were bacterial?


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Offlinelookintolearn
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Re: Cultivation General Discussion [Re: replyom]
    #26880220 - 08/14/20 02:07 PM (3 years, 5 months ago)

I wanted to ask, Does this seems like a decent enough fruiting chamber for this small little guy? It has a total of 6 holes for FAE.



I have other tubs going not worried to much about them but since I spawned this in a half bottle I wasn't to sure. I just wanted some earlier fruits  :psychsplit:


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Looking to start growing? Read through Bod's Introduction to Everything
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InvisibleHobbit GDF
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Re: Cultivation General Discussion [Re: replyom] * 1
    #26880226 - 08/14/20 02:12 PM (3 years, 5 months ago)

Here is photo dump of sorts.
Thank you @ one of us

Here is a cluster. Tailfeathers clone of a pin in a clone agar plate.
So clone of a clone but second was a plate pin.
TAILFEATHERS

More.




Here are

WHITE TAILFEATHERS



Here's some kind of

LIONSMANE


PENIS ENVY


PE6


Also
Playing with some autos


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InvisibleJosex
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Re: Cultivation General Discussion [Re: Hobbit GDF]
    #26880238 - 08/14/20 02:18 PM (3 years, 5 months ago)

That cluster in the first pic is freakin gorgeous.


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Re: Cultivation General Discussion [Re: Josex]
    #26880281 - 08/14/20 02:57 PM (3 years, 5 months ago)

Lol the pic Josex talkin bout looks like there’s a hamster on ur hand poking his head out


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InvisiblePastywhyteMDiscord
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Re: Cultivation General Discussion [Re: replyom]
    #26880308 - 08/14/20 03:12 PM (3 years, 5 months ago)

Quote:

replyom said:


Anyone know what's going on here? Around day 10 post-spawn these "pins" started to appear - at least I thought they were pins. But now it's clear that they are something else - just masses of fruiting body with no head. Y'all seen this before?




That actually looks like the dry bubbles associated with Lecanicillium fungicola. I would keep an eye on that, do not mist it and keep it away from other grows until you’re sure.


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