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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 21 days
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Re: Cultivation General Discussion [Re: A.k.a]
#26846181 - 07/26/20 01:52 PM (3 years, 6 months ago) |
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Automation is unmodified tubs in a room near a window that gets daylight. Lol cubes will grow in the worst conditions.
Pans and tamps imo do require some maintenance.
Damn I want to get back to growing, got to much shit going on.
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
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Re: Cultivation General Discussion [Re: mushboy]
#26846384 - 07/26/20 03:57 PM (3 years, 6 months ago) |
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Quote:
mushboy said:
Quote:
Edmunter said: Who has experience of growing PE#6 and please do tell.......
Grows like a cube. Required no extra attention.

and are they PE strong?
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: Edmunter]
#26846422 - 07/26/20 04:35 PM (3 years, 6 months ago) |
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Nah
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TedsDead



Registered: 01/03/17
Posts: 4,998
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Re: Cultivation General Discussion [Re: Sockadin] 1
#26846437 - 07/26/20 04:43 PM (3 years, 6 months ago) |
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Quote:
Sockadin said: Automation is unmodified tubs in a room near a window that gets daylight. Lol cubes will grow in the worst conditions.
Pans and tamps imo do require some maintenance.
Damn I want to get back to growing, got to much shit going on.
Theyll grow in the worst conditions but not optimally andyields will suffer. Optimize conditions, optimize yields
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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Srirachi
Mold Hand



Registered: 10/18/05
Posts: 11,411
Loc: Fare Thee Well.
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Re: Cultivation General Discussion [Re: Sockadin]
#26846534 - 07/26/20 06:09 PM (3 years, 6 months ago) |
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Quote:
A.k.a said: I just want to point out automation is pretty much useless when you’re just starting out. Once you’ve got a feel for what the conditions should be then it could be helpful if you’re not able to maintain them on their own.
Excellent point. I didn't notice the newness of the person asking.Quote:
Sockadin said: Automation is unmodified tubs in a room near a window that gets daylight. Lol cubes will grow in the worst conditions.
Yeah, I suppose if I had a monotub I might think that. Also, what Ted said.
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jackrabbitcytoplas
Stranger

Registered: 01/30/20
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Re: Cultivation General Discussion [Re: Srirachi]
#26847131 - 07/27/20 04:28 AM (3 years, 6 months ago) |
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Looking for opinions. If I PC a short food saver container with an open agar plate inside of it, then in a SAB place a mushroom cap gill side up next to the plate, never going over the plate, close the box carefully and wait for the cap to drop spores. Would spores reach the plate? Not sure how forcefully they are ejected. Would it be enough to create an axenic plate?
I'm planning on trying this myself, but looking for opinions of experienced cultivators on what will/could happen if I do it right.
edit reason: I wrote aseptic instead of axenic
Edited by jackrabbitcytoplas (07/27/20 04:37 AM)
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Wall.E
Bacteria's Bitch



Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
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Well if my plates get contaminated from bacteria and other spores while I try to remain as sterile as possible, then I guess your plan could work.
I'm not sure if it's the most efficient method, but worth a shot at least
-------------------- Life’s shit, but I’m loving it
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icetech



Registered: 08/21/17
Posts: 3,450
Loc: FSM's loving noodles.
Last seen: 3 months, 5 days
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Re: Cultivation General Discussion [Re: Edmunter]
#26847288 - 07/27/20 07:42 AM (3 years, 6 months ago) |
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Quote:
Edmunter said: Who has experience of growing PE#6 and please do tell.......
Pe6 is my go to.. the agar is aggressive, it colonized fast and are the easiest cubes i have grown..
My last box.. which was less than 2 weeks (fast for me) spores popped which sucked.. went to work and they were like 1" tall came home to this..
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Edited by icetech (07/27/20 07:42 AM)
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Sherlock Shrooms
Neophyte



Registered: 06/25/19
Posts: 606
Loc: Under the Sun
Last seen: 2 years, 10 months
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Re: Cultivation General Discussion [Re: icetech]
#26847640 - 07/27/20 10:51 AM (3 years, 6 months ago) |
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What do you guys think of this bag of spawn? It's got yellow metabolites all the way around it. It's about 4 quarts of rye berries and I used a half quart of spawn to inoculate. It took about 2 weeks to colonize at 68 to 69 degrees. Someone recommended I use a full quart to inoculate the next round for faster colonization but thought I'd share this anyway just to gauge how bad this bag looks, I'm really not sure what a normal amount of metabolites is for bags or if one even sees them at all when things are super healthy. There's a bit of wet spot at the bottom of the pic too.
 This is the block of spawn broken in half, you can still see metabolites here and there throughout the center
-------------------- "When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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its fine... maybe a tad sick with bacteria or something send it.
maybe not g2g but definitely can spawn that.
Edited by sandman420 (07/27/20 10:54 AM)
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Sherlock Shrooms
Neophyte



Registered: 06/25/19
Posts: 606
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Re: Cultivation General Discussion [Re: sandman420]
#26847667 - 07/27/20 10:59 AM (3 years, 6 months ago) |
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Something ain't right. I made a bunch of these bags and spawned them to tubs and just threw out 3 tubs before they even made pins, and others are pinning nicely, and others are pinning not so nicely. This shit keeps happening to me. I lose about half of everything I do each run. Most of these tubs will contam near the end of the first flush if they behave the way every single run has for the past few years. I keep trying everything to get better results, went back to pasteurization didn't help, made thinner subs didn't help, went under field capacity for the sub didn't help, bombed the grow room to reduce spore load didn't help, and a hundred other things. Have done test plates and jars and they never contam. Now I've got new stuff from spores colonizing and hope that goes better. This shit has been going on for a few years. I use a flow hood and am crackheadedly sterile with everything. I haven't see a visible contam in agar or in the spawn for probably a year aside from a small amount of wet spot occasionally on the second grain expansion. Pretty frustrating..
-------------------- "When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

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CrashTest
Stay Learning


Registered: 06/21/20
Posts: 623
Last seen: 6 months, 30 days
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Re: Cultivation General Discussion [Re: sandman420]
#26847670 - 07/27/20 11:00 AM (3 years, 6 months ago) |
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Just spawned my first shoebox, gonna forget about it for a week

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this account is automated, any posts related to activities or advice thereof are strictly opinions from numerous online sites and are for informational purposes only - CrashTest2020
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Quote:
Sherlock Shrooms said: Something ain't right. I made a bunch of these bags and spawned them to tubs and just threw out 3 tubs before they even made pins, and others are pinning nicely, and others are pinning not so nicely. This shit keeps happening to me. I lose about half of everything I do each run. Most of these tubs will contam near the end of the first flush if they behave the way every single run has for the past few years. I keep trying everything to get better results, went back to pasteurization didn't help, made thinner subs didn't help, went under field capacity for the sub didn't help, bombed the grow room to reduce spore load didn't help, and a hundred other things. Have done test plates and jars and they never contam. Now I've got new stuff from spores colonizing and hope that goes better. This shit has been going on for a few years. I use a flow hood and am crackheadedly sterile with everything. I haven't see a visible contam in agar or in the spawn for probably a year aside from a small amount of wet spot occasionally on the second grain expansion. Pretty frustrating..
you have any pictures of some of the tubs going bad?
Where and what kind of contams?
Also pics of the agar plates that you make your mother cultures from.
Edited by sandman420 (07/27/20 12:08 PM)
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Sherlock Shrooms
Neophyte



Registered: 06/25/19
Posts: 606
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Yea here are pics of the few I just threw out, and a pic of one that is pinning nicely and not showing any contams, there are more than one pinning nicely but I'm having a hard time uploading pics so I'll just include the one for now. Some are totally contaminated before any pins show up, and others put out rocking pinsets. This is what's been going on for a long time, like 2 years. This run I have a bunch of tubs of 2 different cultures and they're completely different varieties, same treatment and process for everything all the way through.. I have started different stuff from spores and never see any contams in the agar or spawn, and yet when the tubs contam it's always the same exact green around the same time. I'd think if my sterile technique was bad I'd see pandora's box of contamination like I used to when I started growing. If it was a hidden contam on my agar I'd think the different varieties I start from spores would have different problems or none at all. In the beginning like 4 years ago I'd see rhizopus, aspergillus, penicillum, cobweb, etc. Now it's always the same exact contam every time. Some make it to the end of the second flush and most crap out somewhere between the middle of the first flush and the end of the second. Stumped 


-------------------- "When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

Edited by Sherlock Shrooms (07/27/20 12:29 PM)
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Camera93
We got dicks like Jesus



Registered: 08/15/18
Posts: 3,224
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how are you inoculating your bags?
and how long and what psi are you sterilizing at?
have you taken your inoculate to agar to test?
-------------------- All I need are some tasty waves, a cool buzz, and I’m fine. Whatever you decide won’t really impact our survival Close your eyes, and do the best that you can
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Sherlock Shrooms
Neophyte



Registered: 06/25/19
Posts: 606
Loc: Under the Sun
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Since the contams are identical in the different cultures I bombed the room thinking it may have been spore load of a particular type but that didn't help. I load the tubs carefully in front of the flow hood with gloves on but that doesn't seem to help. Test plates that I've made black transfers into never contam, nor do grain jars that I do blank transfers into ever contam either. I vent the PC 20 minutes and cook jars for 2.5 hours at 18 psi and bags 3.5 hours at 18 psi. Proper synthetic filter disks for jars and unicorn .2 micron bags. I even tried properly pasteurizing my CVG between 140 and 150 for an hour this time and that didn't help, I know everyone says it's pointless but after you've lost as much as I have you'll try anything.. I have brand new cultures on their 4th transfer from spore going right now and I'm about to drop them into grains but I'm not very hopeful of that doing any better because I've already gone through this with new cultures so many times there's no reason to think this time it will change anything. This is crazy. SOS
-------------------- "When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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One possibility is you have trich/aspergillus/penicillium that has gone myco-parasitic on the mycelium of your master cultures and/or prints. You would need a scanning electron microscope to really see the interaction.
Or your room is heckin' dirty and when you are cooling the coir blocks it is simply sucking in a assload of trich or whatver mold that is since it has become the dominant mold in your little ecosystem. In short your room be dirty and needs to be nuked, ozoned, bleached, carpet removed, etc. But I now see you said you have tried cleaning. Maybe didnt clean the vector. Moldy backyard always wet maybe? Maybe you just didnt properly disinfect as much as just clean.
Edited by sandman420 (07/27/20 12:39 PM)
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A.k.a
Stranger



Registered: 10/27/19
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Re: Cultivation General Discussion [Re: sandman420] 1
#26847848 - 07/27/20 12:40 PM (3 years, 6 months ago) |
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You could try using more spawn.
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LAGM2020     
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Camera93
We got dicks like Jesus



Registered: 08/15/18
Posts: 3,224
Last seen: 8 hours, 28 minutes
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its gotta be in the spawn tho right? even in a huge moldy house, you would expect it lose to mold after a flush. But the myc shouldn't have a problem colonizing the substrate if its clean right?
have you ever saved any of your spawn to take to agar?
@sandman, i think its important for you to know I read your comments as tho its Dwight saying them lol im sure he pry has some magic on schrute farms
-------------------- All I need are some tasty waves, a cool buzz, and I’m fine. Whatever you decide won’t really impact our survival Close your eyes, and do the best that you can
Edited by Camera93 (07/27/20 12:44 PM)
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Sherlock Shrooms
Neophyte



Registered: 06/25/19
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I inoculate everything in my 2 foot x 3 foot home made flow hood. I have tested tons of blank plates and jars in front of it and there are no problems. Whether bags or jars I load them directly into my flow hood from the PC, and whatever plates or grain masters I'm using I wipe down thoroughly with alcohol and do all my work right in front of the filter. My hands never come between the filter and the work. I flame sterilize with a map gas propane torch. Honestly I only recently started using bags and the problem didn't get better or worse. I'm going back to all jars next run until I can figure this out as I know bags will only amplify problems. I know many contams won't show in spawn but at this point and after this epic quantity of work I've done if my technique was bad once in a while I'd see a contam in my spawn and it's been well over a year since I've seen one jar or bag show a visible contam. You can probably imagine after all these losses how careful I am with sterile work. This is my flow hood.
-------------------- "When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

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