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badsponge

Registered: 12/20/18
Posts: 872
Loc: SoCal
Last seen: 11 months, 9 days
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Re: Cultivation General Discussion [Re: natedawgnow]
#26739564 - 06/12/20 12:58 PM (3 years, 7 months ago) |
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Quote:
natedawgnow said: If you're getting mad condensation and pools, it would behoove you to increase FAE rather than decrease your water content.
Yeah, I started by adding holes to my latest tub I spawned about a week ago. They're making a big difference. I would've been pouring off standing water by now in an unmodded tub. I tried to make unmodded tubs work for a long time, due to what I read in Bod's and Caps' teks. They just don't work for me. I should've tried tub holes long ago.
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Nichrome
I'm a torso!



Registered: 12/17/18
Posts: 6,491
Loc: Zone 5
Last seen: 20 hours, 10 minutes
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Re: Cultivation General Discussion [Re: c10h12n2o]
#26739567 - 06/12/20 01:00 PM (3 years, 7 months ago) |
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Quote:
Psyche delics said:
Quote:
Nichrome said: This plate:

produced these mammajammas:

Why do big fruits produce that wrinkled look?
It's just phenotype expression. These clones always look like this. Have seen big fruits with the typical cube look in other cultures. I like to hunt out unique pheno's. They're just fancy pure bred chickens....
Quote:
c10h12n2o said: What kinda culture is that nichrome?
It's a clone from a Burmese culture. Many of the original fruits were very fat and squat like UFO's. All the fruits are solid all the way through and very strong. At first this clone was worrisome as the pinset is sporadic and slow to get going but the weight in the end was better than average overall, just not as many mushrooms as far as numbers go. Some of them get really big (corner to corner in a 64 qt tub). This culture was banked as a keeper. Very kind folks from The Tribe contributed the spores.
The BCT (Burma Clone Thick) culture is from the one on the right in the photo (original MS specimen):
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
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Spreadtheknowledge
Worker


Registered: 04/09/19
Posts: 237
Loc: Earth
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Re: Cultivation General Discussion [Re: Nichrome]
#26739609 - 06/12/20 01:22 PM (3 years, 7 months ago) |
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If I start with an Ms tub and I clone a nice fruit and then do a tub with that clone then take a clone from the nicest fruit of that tun how many times should I keep doing that until the genetics start getting shitty I've heard about 5 times any opinions?
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poisoned
untitled



Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 1 year, 3 months
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Most people only clone once. I wouldn’t really do it multiple times unless you visibly see different genetics.
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Culliganman360
Stranger



Registered: 06/05/19
Posts: 854
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Re: Cultivation General Discussion [Re: Nichrome]
#26739623 - 06/12/20 01:27 PM (3 years, 7 months ago) |
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Quote:
Nichrome said: This plate:

produced these mammajammas:

Nice fruits playa
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Spreadtheknowledge
Worker


Registered: 04/09/19
Posts: 237
Loc: Earth
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Re: Cultivation General Discussion [Re: poisoned]
#26739982 - 06/12/20 04:32 PM (3 years, 7 months ago) |
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So instead of cloning multiple times if I want to make a strain faster or something like this I should clone once and then do segment transfers on agar than test each transfer?
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Spreadtheknowledge
Worker


Registered: 04/09/19
Posts: 237
Loc: Earth
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Or should I keep doing Ms tubs and test clones from there?
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Roger Clemency
Smile


Registered: 03/23/20
Posts: 2,005
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You clone a mushroom and grow that out on agar, when it's ready you transfer to grains or whatever but also save that agar plate in the fridge. If the clone does well and you want to keep it then you put the culture in a slant to save it. That's the basic premise.
Check out the thread called Master and Slaves about saving cultures and a safe way to keep them alive and limit risk.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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Spreadtheknowledge
Worker


Registered: 04/09/19
Posts: 237
Loc: Earth
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Ok but basically what im asking is you cant make a strain much better by cloning a clone can you?
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FriedEgg



Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
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what kind of lysol do you guys use to disinfect your work table or work space? with alcohol being hard to find now, i'd like to start using lysol to clean my table before working in front of my flow hood but there are so many kinds of lysol. from what i understand, lysol is a brand that makes many products.
and do you mix it with water? or just use it undiluted?
it's not this one, is it? this one looks like something you're supposed to spray up in the air, not on surfaces
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FriedEgg



Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
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Quote:
Spreadtheknowledge said: Ok but basically what im asking is you cant make a strain much better by cloning a clone can you?
no, don't clone a clone. that'll only make it worse, not better. look up "senescence"
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 2 hours, 3 minutes
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Re: Cultivation General Discussion [Re: FriedEgg]
#26740532 - 06/12/20 08:26 PM (3 years, 7 months ago) |
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There’s really nothing you can do to make a culture better. You just gotta keep narrowing genetics down some and hoping you get something worth keeping. If not you dump it and start over.
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LAGM2020     
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Spreadtheknowledge
Worker


Registered: 04/09/19
Posts: 237
Loc: Earth
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Re: Cultivation General Discussion [Re: A.k.a]
#26740589 - 06/12/20 08:52 PM (3 years, 7 months ago) |
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Ok this makes more sense now but one thing I dont see answered anywhere is say I clone a fruit from an ms tub and it makes really nice flushes but that clone on the agar is most likely not an isolated strain so when I save it to a master plate or slant when I want to use it again to make another slave plate how do I go about it if different parts of the plate/slant have different strains on them it wont produce the same as the first clone tub did that gave good flushes or if i leave the plate or slant for long enough will they sort of mesh in with eachother?
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 2 hours, 3 minutes
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No you’re right it’ll change when you transfer from it.
I was messing around with this a little recently and just decided that once you take a clone and transfer a few times it’s narrowed down enough that hopefully it won’t change too drastically.
Clones seem to be pretty consistent after a few transfers the few times i kept one going.
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LAGM2020     
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Spreadtheknowledge
Worker


Registered: 04/09/19
Posts: 237
Loc: Earth
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Re: Cultivation General Discussion [Re: A.k.a]
#26740606 - 06/12/20 09:00 PM (3 years, 7 months ago) |
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Ok interesting so let's say I take a piece of fruit and put it to agar and then knock up a grain jar to test in a shoebox from the plate that has the piece of fruit on it how many more agar transfers do you recommend before making a master slant to save permanently?
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 2 hours, 3 minutes
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I have no idea. Four I guess is where it was pretty consistent.
There’s only been one clone I ran with jars from the tissue plate and then t3-4s later on and there was a huge difference between them. The transferred ones were way more uniform. Just a tub full of identical shrooms. The tissue plate tub was more varied like ms.
I really don’t have enough experience working with clones for long periods though. I usually run a couple then move to a new variety right now cuz I have a ton of new ones.
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LAGM2020     
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Spreadtheknowledge
Worker


Registered: 04/09/19
Posts: 237
Loc: Earth
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Re: Cultivation General Discussion [Re: A.k.a]
#26740657 - 06/12/20 09:35 PM (3 years, 7 months ago) |
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Well thanks alot for the info man that's really helpful I'm making a book right now not for the public lol just personal referance I'm going to have to redo my save and master part to include a 4 transfers before storing master plates and slants permanently
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 2 hours, 3 minutes
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lol that’s just from the little bit of stuff I’ve done man don’t lock it in.
There’s tons of people with way more experience with this stuff.
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LAGM2020     
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Spreadtheknowledge
Worker


Registered: 04/09/19
Posts: 237
Loc: Earth
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Re: Cultivation General Discussion [Re: A.k.a]
#26740695 - 06/12/20 09:53 PM (3 years, 7 months ago) |
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Do u know anyone I could pm with that same question?
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: A.k.a]
#26740696 - 06/12/20 09:54 PM (3 years, 7 months ago) |
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dont lock anyone in on anything just take notes that you can reference later
besides everyone knows you do 5 transfers for super mega dank potent dealer grade slants.
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