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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 3 hours, 21 minutes
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Re: Cultivation General Discussion [Re: c10h12n2o]
#26738861 - 06/12/20 07:13 AM (3 years, 7 months ago) |
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I’ve been learning about growing peppers lately, and there’s an interesting crossover with mushrooms.
The hottest peppers ever made all have wrinkled skin. That also seems to be the case with cubes. I doubt it’s the same mechanism but with peppers it said it’s because wrinkles create more surface area.
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LAGM2020     
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Gastronomicus
3-0-G



Registered: 03/31/05
Posts: 9,727
Last seen: 2 hours, 33 minutes
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Re: Cultivation General Discussion [Re: A.k.a]
#26738879 - 06/12/20 07:24 AM (3 years, 7 months ago) |
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Nice, I'm growing peppers too. Some Rocotos.
-------------------- Make my Funk the P Funk, I wants to get Funked up
LAGM2024
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ManifoldPrime
Per Ardua Ad Astra



Registered: 03/16/17
Posts: 1,313
Loc: South Africa
Last seen: 1 year, 1 month
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I got chilli and pepper seedlings in pots right now sometimes garden mushies poke up through the soil after watering
-------------------- My Magnum Opus:Thai Variety Comparison Please correct me if I say something wrong! | Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |
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Cucumelo
High hopes!


Registered: 01/05/20
Posts: 8
Last seen: 2 years, 1 month
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Re: Cultivation General Discussion [Re: MH5109]
#26738921 - 06/12/20 07:46 AM (3 years, 7 months ago) |
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Hi all, could anyone tell me if they have any comments, thoughts about my substrate? What do you think? Any feedback? It's pretty consolidated and pins are showing. Also, hundreds of primordia. I noticed the FAE was too much, judging by how long it's taking to grow. So, I filled those holes in the tub with cotton (I don't have polyfill). Hopefully, they'll pick up now with more humidity. I had four 32 oz. jars fully colonized (after 3 months). The strain is "Menace," and I used wheat grain as a medium, mixed in with Coco coir and vermiculite (3/1). Everything looks good to me, so far but, I'm a newbie. So, I'm looking for feedback. This is my second project. My first project was BRF cakes, and was a success -though potency was low. Then again, it may have been my weight that kept the psilocybin from fully reaching my seratonin reactors (I've gained a few lbs.). Anyway, the last flush was pretty potent. Thanks to all for posting your experiences here. I've learned so much from you!
P.S. I have a question about culture. I'm planning to get a few prints from the caps, once the fruits come in. However, I see some healthy mycelium in this substrate that I'd like to cut away and keep in a petri dish. Is beer yeast the only way to keep the culture alive in a glass receptacle? Is there another medium that I could use to keep the mycelium stored until I decide to use again? -Thanks!
All my best, Cucumelo






Edited by Cucumelo (06/12/20 12:39 PM)
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ManifoldPrime
Per Ardua Ad Astra



Registered: 03/16/17
Posts: 1,313
Loc: South Africa
Last seen: 1 year, 1 month
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Re: Cultivation General Discussion [Re: Cucumelo]
#26739062 - 06/12/20 09:00 AM (3 years, 7 months ago) |
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@cucumelo Everything looks dandy, although next time maybe level your sub out with a ruler or something when spawning, looks a lil bumpy. Theres beads of moisture on the surface from the looks of it. Dont baby it, it looks lovely as is.
Quote:
I'm planning to get a few prints from the caps, once the fruits come in. However, I see some healthy mycelium in this substrate that I'd like to cut away and keep in a culture. Is beer yeast the only way to keep the culture alive in a glass receptacle? Is there another medium that I could use to keep the mycelium stored until I decide to use again? -Thanks!
Print, and take some tissue samples from pins/fruits just after harvesting, NOT the substrate myc. perform a "biopsy":
https://www.shroomery.org/forums/showflat.php/Number/25915460#25915460
I cant seem to find a tek for this despite searching three times though. Essentially you peel back some layers of mush flesh (usually the stem) then use your scalpel to get some of that meat inside (around the size of a grain) and put that on agar.
-------------------- My Magnum Opus:Thai Variety Comparison Please correct me if I say something wrong! | Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |
Edited by ManifoldPrime (06/12/20 09:01 AM)
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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 14 days, 58 minutes
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Well I can't find the TEK either, had some great pics...

prints! OG SPORE PRINT TEK™
Edited by Inthepit (06/12/20 09:37 AM)
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badsponge

Registered: 12/20/18
Posts: 872
Loc: SoCal
Last seen: 11 months, 9 days
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Re: Cultivation General Discussion [Re: Becker1996]
#26739184 - 06/12/20 10:06 AM (3 years, 7 months ago) |
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Quote:
Becker1996 said: I used about 650 grams of coco coir brick, 2 quarts of vermiculite, 4 litre of water and 1.5kg of oat spawn and it was spawned about 7 days ago
Too much water. I use almost the same recipe but 1.8L water, 1.5L verm.
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Cultivation General Discussion [Re: badsponge]
#26739189 - 06/12/20 10:08 AM (3 years, 7 months ago) |
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1.8L is barely any water for a full sized tub. Thats like super dry.
I use just under 3L water per brick of coir and 1qt verm.
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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 14 days, 58 minutes
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Re: Cultivation General Discussion [Re: badsponge]
#26739234 - 06/12/20 10:28 AM (3 years, 7 months ago) |
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Quote:
badsponge said:
Quote:
Becker1996 said: I used about 650 grams of coco coir brick, 2 quarts of vermiculite, 4 litre of water and 1.5kg of oat spawn and it was spawned about 7 days ago
Too much water. I use almost the same recipe but 1.8L water, 1.5L verm.
I use: coir, 650g x 1.864 mL/g = 1211.6 mL water, for 5 jars coir
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badsponge

Registered: 12/20/18
Posts: 872
Loc: SoCal
Last seen: 11 months, 9 days
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Re: Cultivation General Discussion [Re: natedawgnow]
#26739236 - 06/12/20 10:28 AM (3 years, 7 months ago) |
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Quote:
natedawgnow said: 1.8L is barely any water for a full sized tub. Thats like super dry.
I use just under 3L water per brick of coir and 1qt verm.
I started out with 3.25L. But I kept getting LOTS of condensation and pooling water every day. I kept dialing it back little by little trying to get it under control. I got it all the way down to 1L per brick, and within a few days I was still getting standing water. I've been using Caps' SOS tek with unmodded tubs. I recently decided to try adding holes in my latest tub, and follow Caps' sub recipe which calls for 2qt (1.8L). That tub is 4 days old, reasonable condensation, no pooling water, and it's colonizing quickly. I think I'm finally on track for a set-it-and-forget-it tub.
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Spreadtheknowledge
Worker


Registered: 04/09/19
Posts: 237
Loc: Earth
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Re: Cultivation General Discussion [Re: badsponge]
#26739418 - 06/12/20 11:42 AM (3 years, 7 months ago) |
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What's the humidity in the room your doing this in 1l seems crazy low
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Cucumelo
High hopes!


Registered: 01/05/20
Posts: 8
Last seen: 2 years, 1 month
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@ManifoldPrime
Hi there, So great to get some feedback! I'm happy that you think all is dandy, and I realize I didn't do too good a job at laying the substrate down. Hence the bumpiness. -Okay so, I have "Agar Agar powder" that I bought at an Asian spice store, and I have a petri dish that came with my microscope. But, the Agar resembles white flour, and all the petri dishes with culture -that I've seen on this message board- seem to have a moist substance. So, am I to add water or any other element to the agar, before inserting the flesh from the "biopsy?" What am I missing?
Thanks again, this was really uplifting! Cucumelo
Edited by Cucumelo (06/12/20 12:03 PM)
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roarkell
The Roar Master!



Registered: 08/29/19
Posts: 2,223
Loc: Southern WI
Last seen: 6 months, 22 days
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Re: Cultivation General Discussion [Re: Cucumelo]
#26739433 - 06/12/20 11:48 AM (3 years, 7 months ago) |
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Lately I've been doing 600g coir 400g verm and 4 litres of water... haven't had any issues yet!
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ManifoldPrime
Per Ardua Ad Astra



Registered: 03/16/17
Posts: 1,313
Loc: South Africa
Last seen: 1 year, 1 month
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Re: Cultivation General Discussion [Re: roarkell]
#26739477 - 06/12/20 12:11 PM (3 years, 7 months ago) |
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@cucumelo
https://www.shroomery.org/forums/showflat.php/Number/24330957/page/1/fpart/1
If that dish is a borosilicate or such glass dish, its a reusable one, but I'd suggest getting disposable dishes and following that tek. Read the whole OP! Then ask yourself how you can do it, then read it again!
-------------------- My Magnum Opus:Thai Variety Comparison Please correct me if I say something wrong! | Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: Cultivation General Discussion [Re: Cucumelo]
#26739483 - 06/12/20 12:14 PM (3 years, 7 months ago) |
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Quote:
Cucumelo said: @ManifoldPrime
Hi there, So great to get some feedback! I'm happy that you think all is dandy, and I realize I didn't do too good a job at laying the substrate down. Hence the bumpiness. -Okay so, I have "Agar Agar powder" that I bought at an Asian spice store, and I have a petri dish that came with my microscope. But, the Agar resembles white flour, and all the petri dishes with culture -that I've seen on this message board- seem to have a moist substance. So, am I to add water or any other element to the agar, before inserting the flesh from the "biopsy?" What am I missing?
Thanks again, this was really uplifting! Cucumelo
Your missing out on some reading. This is the most basic of basics. You must read up before attempting agar. Yes you need water as well as a source of nutrients. You will get there but it's gonna take some work.
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Cucumelo
High hopes!


Registered: 01/05/20
Posts: 8
Last seen: 2 years, 1 month
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@ManifoldPrime
Excellent! I see my questions about agar will be answered by that post. I will do as you've suggested: Read, contemplate -then, read again. The petri dish I have is transparent plastic. Not very good quality but, closed-shut pretty well... Maybe I better invest in a professional, glass petri dish (or disposable petri dishes?). The microscope I bought is more of a toy than an scientific instrument, but very affordable. In short: Cheap.
Thanks again! Cucumelo
Edited by Cucumelo (06/12/20 12:34 PM)
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Cucumelo
High hopes!


Registered: 01/05/20
Posts: 8
Last seen: 2 years, 1 month
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Re: Cultivation General Discussion [Re: gizmo1]
#26739513 - 06/12/20 12:32 PM (3 years, 7 months ago) |
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@gizmo1
I will read-up on this as I'm keenly interested in keeping my own supply of spores and culture.
That is: Spores from prints and culture from the inner-stem-flesh biopsy.
Thank you all for the feedback!
Cucumelo
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Nichrome
I'm a torso!



Registered: 12/17/18
Posts: 6,491
Loc: Zone 5
Last seen: 21 hours, 27 minutes
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Quote:
Spreadtheknowledge said: For storing myc in slants does anyone think it would be better to use charcoal in the agar as I've heard it has antibacterial properties anyone have an opinion about this?
As long as you use parafilm properly and you're work is clean you should be fine without additives.
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Cultivation General Discussion [Re: badsponge]
#26739536 - 06/12/20 12:44 PM (3 years, 7 months ago) |
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Quote:
badsponge said:
Quote:
natedawgnow said: 1.8L is barely any water for a full sized tub. Thats like super dry.
I use just under 3L water per brick of coir and 1qt verm.
I started out with 3.25L. But I kept getting LOTS of condensation and pooling water every day. I kept dialing it back little by little trying to get it under control. I got it all the way down to 1L per brick, and within a few days I was still getting standing water. I've been using Caps' SOS tek with unmodded tubs. I recently decided to try adding holes in my latest tub, and follow Caps' sub recipe which calls for 2qt (1.8L). That tub is 4 days old, reasonable condensation, no pooling water, and it's colonizing quickly. I think I'm finally on track for a set-it-and-forget-it tub.
If you're getting mad condensation and pools, it would behoove you to increase FAE rather than decrease your water content.
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badsponge

Registered: 12/20/18
Posts: 872
Loc: SoCal
Last seen: 11 months, 9 days
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Quote:
Spreadtheknowledge said: What's the humidity in the room your doing this in 1l seems crazy low
I know it's crazy low. I'm in SoCal, so humidity is not an issue. Putting holes in my tubs and lids seems to be working much better at managing moisture, and I'm adding 1.8L now, per Caps' SOS tek.
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