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poisoned
untitled



Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 1 year, 3 months
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Quote:
Spreadtheknowledge said: is it somehow possible to clone with a dried out mushroom I'm thinking maybe I can take a shortcut buy a bag and take a piece of one to agar
Only if they weren't dried correctly
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TedsDead


Registered: 01/03/17
Posts: 4,998
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Re: Cultivation General Discussion [Re: poisoned]
#26730412 - 06/09/20 02:11 AM (3 years, 7 months ago) |
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-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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MLPismyOPSEC
That One Ponyfucker


Registered: 11/13/18
Posts: 884
Loc: Equestria? Mordor? Wester...
Last seen: 11 days, 14 hours
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Re: Cultivation General Discussion [Re: TedsDead]
#26730701 - 06/09/20 07:49 AM (3 years, 7 months ago) |
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@ Radsquatch; yeah that tub is dry as hell. I'd give it 2-3 light mistings today, til you see all the light brown coir turn a darker brown. Should be a walnut brown.
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Nichrome
I'm a torso!



Registered: 12/17/18
Posts: 6,492
Loc: Zone 5
Last seen: 3 hours, 6 minutes
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Re: Cultivation General Discussion [Re: Yeetusdeetus]
#26730828 - 06/09/20 09:01 AM (3 years, 7 months ago) |
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Quote:
Yeetusdeetus said:
Quote:
Nichrome said: Cloned a Ganoderma Tsugae I found on a log in the woods. Swiped some wild tarragon smelling oyster mushroom spores on agar a few days ago. They are sprouted now and looking good so far. Cubes and stuff too yadda yadda yadda. Life is good.
How do you go about getting fruiting strains from spores? I’ve always heard edibles were easier with live cultures and clones.
Don't worry about the masses of rude gatekeepers...
Basically I just xfer (usually once or twice) till I have a clean plate then I take a wedge and make an LC from the first clean plate to test out how a culture does with a lot of diversity (most are just fine). I then proceed if it still seems interesting to separate out colonies on different plates until I have "non competitive colonies" to work with and make an LC from each of those for trial runs. Once the trials have finished I bank 10 Ml vials of the winner LC in the fridge for future use and the rest get balked. One drop of LC from the bank will make a new plate which can make gallons of new LC within a matter of weeks for production runs.
My LC recipe is 1 TBSP organic rice flour (I prefer white but brown is fine as well as other flours like millet, Rye, etc) and a pinch of peptone per 600 Ml of water in a quart mason jar PC'd for 15 minutes. This works well with most fungi I have encountered.
Each mushroom has it's own preferences. Not much difference between psychoactive and edible/medicinal fungi. IME clones and MS colonies are equal with their pro's and con's and there is no "set in stone" rule about which one works better.
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
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poisoned
untitled



Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 1 year, 3 months
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Re: Cultivation General Discussion [Re: Nichrome]
#26730999 - 06/09/20 10:05 AM (3 years, 7 months ago) |
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My lesson of the month:
Don't use low nutes agar for plates you're planning to transfer to grains. Shit's slow to jump off. I got to do some experiments with various nute levels to find the optimum.
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Gan
Wielder of Narya



Registered: 08/26/19
Posts: 927
Loc: Valinor
Last seen: 5 months, 28 days
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Re: Cultivation General Discussion [Re: poisoned]
#26731038 - 06/09/20 10:24 AM (3 years, 7 months ago) |
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I'm about to start cutting, staining, and finishing my wood for my flow hood. Normally for something like this I'd just stain and finish then cut cause I'm mostly finishing the wood to stop contaminants/mold growing on my hood and finishing one large sheet is a lot easier than 15 smaller pieces. If I stain first, it means the cut edges wont be finished and thus open to mold growth. Does anyone think this would be an issue?
Would it be better to cut or stain first?
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badsponge

Registered: 12/20/18
Posts: 872
Loc: SoCal
Last seen: 11 months, 9 days
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Re: Cultivation General Discussion [Re: Gan]
#26731048 - 06/09/20 10:28 AM (3 years, 7 months ago) |
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I'd finish the whole sheet, cut, then re-finish the edges.
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Sherlock Shrooms
Neophyte



Registered: 06/25/19
Posts: 606
Loc: Under the Sun
Last seen: 2 years, 10 months
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Re: Cultivation General Discussion [Re: badsponge]
#26731133 - 06/09/20 11:07 AM (3 years, 7 months ago) |
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I've found varying responses in my searches so I'll ask here. With tubs that had nasty green in them do you guys think hosing them out, letting them dry, and then vigorously wiping down the inside with 70% iso is sufficient before loading them again? I've heard Kizzle say before that alcohol just smears spores around more than it actually kills them. Not sure how true that is. Is there any benefit to washing them out with soap and water beyond what I'm already doing?
-------------------- "When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

Edited by Sherlock Shrooms (06/09/20 11:08 AM)
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Yeetusdeetus


Registered: 11/23/19
Posts: 1,242
Last seen: 9 hours, 24 minutes
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Re: Cultivation General Discussion [Re: Nichrome]
#26731182 - 06/09/20 11:31 AM (3 years, 7 months ago) |
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Quote:
Nichrome said:
Quote:
Yeetusdeetus said:
Quote:
Nichrome said: Cloned a Ganoderma Tsugae I found on a log in the woods. Swiped some wild tarragon smelling oyster mushroom spores on agar a few days ago. They are sprouted now and looking good so far. Cubes and stuff too yadda yadda yadda. Life is good.
How do you go about getting fruiting strains from spores? I’ve always heard edibles were easier with live cultures and clones.
Don't worry about the masses of rude gatekeepers...
Basically I just xfer (usually once or twice) till I have a clean plate then I take a wedge and make an LC from the first clean plate to test out how a culture does with a lot of diversity (most are just fine). I then proceed if it still seems interesting to separate out colonies on different plates until I have "non competitive colonies" to work with and make an LC from each of those for trial runs. Once the trials have finished I bank 10 Ml vials of the winner LC in the fridge for future use and the rest get balked. One drop of LC from the bank will make a new plate which can make gallons of new LC within a matter of weeks for production runs.
My LC recipe is 1 TBSP organic rice flour (I prefer white but brown is fine as well as other flours like millet, Rye, etc) and a pinch of peptone per 600 Ml of water in a quart mason jar PC'd for 15 minutes. This works well with most fungi I have encountered.
Each mushroom has it's own preferences. Not much difference between psychoactive and edible/medicinal fungi. IME clones and MS colonies are equal with their pro's and con's and there is no "set in stone" rule about which one works better.
Damn that sounds intense compared to what I’m used to. Is there a specific morphology you look for in “non competitive colonies?” I assume you’d be looking for uniform growth but I’ve been wrong plenty times before. Thanks for sharing your experience dude I’m definitely gonna get some spores from these oysters and add em to my agar rotation next week.
--------------------

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Radsquatch
forestdweller


Registered: 04/24/20
Posts: 20
Last seen: 3 years, 2 months
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Re: Cultivation General Discussion [Re: MLPismyOPSEC]
#26731208 - 06/09/20 11:39 AM (3 years, 7 months ago) |
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Quote:
MLPismyOPSEC said: @ Radsquatch; yeah that tub is dry as hell. I'd give it 2-3 light mistings today, til you see all the light brown coir turn a darker brown. Should be a walnut brown.
Hahaha. Thanks man, I'll do exactly that. Looks like I was a little too successful in keeping everything dry.. I guess I was a little paranoid about being too wet, since that seems to be the typical issue.
-------------------- Discovery is just thinking God's thoughts after him.
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poisoned
untitled



Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 1 year, 3 months
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Spores are not as much of an issue, they're everywhere anyways. I still soak my tubs in bleach tho, but the most important thing is to really scrub all the living cultures out of the tub.
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Gan
Wielder of Narya



Registered: 08/26/19
Posts: 927
Loc: Valinor
Last seen: 5 months, 28 days
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Re: Cultivation General Discussion [Re: Radsquatch]
#26731530 - 06/09/20 01:53 PM (3 years, 7 months ago) |
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So I reached out to FP about a HEPA filter I'm getting. It's a 36"x24". I was asking for a graph of resistance at different values, but instead they said "The recommended CFM for the 36x24 HEPA filter is 896. This is the minimum requirement for proper filtration."
Does that mean it doesn't actually achieve the rated 99.99% at 3 microns filtration unless flow out of the hepa is 896 cfm? Or does it mean it has to be at least 896 CFM pushing from behind the filter, which will then exit the filter at a lower CFM due to the resistance?
Edited by Gan (06/09/20 02:03 PM)
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Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house
Last seen: 6 hours, 46 minutes
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Re: Cultivation General Discussion [Re: Gan]
#26731556 - 06/09/20 02:03 PM (3 years, 7 months ago) |
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They like to pair their filters and blowers. I feel like they don't let you know very much other than, those two work together. I bought my pairing from them, 12 x 24. I find out now my blower could have handled a 24x24. I asked them for dimensions they prefer used, just the depth was what I was asking them about, never got answer. If anybody knows though? I went 17"
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Gan
Wielder of Narya



Registered: 08/26/19
Posts: 927
Loc: Valinor
Last seen: 5 months, 28 days
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Re: Cultivation General Discussion [Re: Crackatoa]
#26731601 - 06/09/20 02:23 PM (3 years, 7 months ago) |
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Yeah I don't even need to know the specs cause I know my blower will work. It's a 1TDU2 (which is what they'd recommend except they sell it with no stickers for like $100 more). But still I just wanted to know how much more powerful my blower was gonna be based off the specs of the filter. Only for curiosities sake really. But then they said that for proper filtration and it kind confused me
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Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house
Last seen: 6 hours, 46 minutes
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Re: Cultivation General Discussion [Re: Gan]
#26731625 - 06/09/20 02:31 PM (3 years, 7 months ago) |
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They didn't change anything on mine. It came in a Dayton box with 1TDU2 all over it. Now I just gotta get me 24x24 filter without wife knowing
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MH5109
Stranger


Registered: 09/27/17
Posts: 1,385
Loc: America
Last seen: 2 years, 11 months
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Re: Cultivation General Discussion [Re: Crackatoa]
#26731634 - 06/09/20 02:34 PM (3 years, 7 months ago) |
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Pe test culture 24hrs after the first shake, looks like its recovering ok.
--------------------
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King_DedeDe
AnonEnsemble

Registered: 02/08/20
Posts: 17
Last seen: 3 years, 5 months
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Re: Cultivation General Discussion [Re: Crackatoa]
#26731637 - 06/09/20 02:35 PM (3 years, 7 months ago) |
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Can someone tell me what's going on here? I've taken a picture of two types of mycelium growth, one of which I'm familiar with - the fuzzy type that grows on the outside of your cakes when birthing or re-emerging from dunks but recently with this leucistic strain I have been finding abnormal thick growth of this yellow tanish fungus I can only guess is some type of sclerotia. Now I did let these cakes colonize in their jars for about two months or more because there were certain spots in the jar that we're not yet colonized. Around the second month I was noticing no change in the uncolonized spot but a new growth formation was taking over the jar which was yellow tanish and was secreting lots of piss.
Edited by King_DedeDe (06/09/20 02:38 PM)
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Gan
Wielder of Narya



Registered: 08/26/19
Posts: 927
Loc: Valinor
Last seen: 5 months, 28 days
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Re: Cultivation General Discussion [Re: Crackatoa]
#26731639 - 06/09/20 02:36 PM (3 years, 7 months ago) |
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Only reason I went with FP was cause they could get it here the fastest and I was tired of spending so much time looking for deals on ebay. I found one place that priced a 24x36 for $278 before shipping which wasn't awful for a brand new hepa of the quality they provide. But it had a 4 week lead time so I bit the bullet to get one here faster.
I am curious though about what they meant about needing 896 CFM for proper filtration.
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 8 hours, 4 minutes
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Re: Cultivation General Discussion [Re: Gan]
#26731644 - 06/09/20 02:39 PM (3 years, 7 months ago) |
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That looks like extremely bacterial myc swelling up
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LAGM2020     
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DonkDonk
Noob


Registered: 04/12/20
Posts: 59
Loc: Ireland
Last seen: 2 years, 11 months
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Re: Cultivation General Discussion [Re: A.k.a]
#26731692 - 06/09/20 03:08 PM (3 years, 7 months ago) |
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How can you tell if your mushrooms are grow real slow or if they have aborted?
-------------------- "Your future hasn’t been written yet. No one’s has. Your future is whatever you make it. So make it a good one."   
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